TY  - CONF
AU  - Lukić, Ivana
AU  - Popović, Mina
AU  - Injac, Ninoslava
AU  - Kovačević, Ana
AU  - Miljković, Radmila
AU  - Stojanović, Marijana
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/868
AB  - Inflammatory bowel diseases (IBDs) are associated with reduced diversity and decreased
contribution of probiotic bacteria within the comensal microbiota. A range of probiotic
bacteria, including the genera Lactobacillus, could be protective in IBDs due to their
capacity to trigger anti-inflammatory mechanisms 1. The aim of the study was to explore
immunomodulatory impact of probiotic bacteria. Lactobacillus reuteri (LR) and its
contribution to the alleviation of severity of a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-
induced colitis in mice. Colitis was induced in outbred Intor Swiss:Albino mice by a single
administration of TNBS dissolved in 50% ethanol (day 0). LR in phosphate buffer solution
(5x106 CFU/mL, p.o.) was given daily, 7 days prior and/or 7 days after day 0 (n=10 per
treatment). Mice subjected to the induction of colitis without LR treatment were referent.
A significant reduction in disease severity was noticed only in group treated by LR prior
and upon colitis induction. The alleviation of disease severity correlated with lessening of
local infiltration of leukocytes, a decrease in local inflammatory response
(myeloperoxidase activity, production of superoxide ions, IL-6, a nd T NFα), a nd a n
increase in local production of IL-10. Presented results show that LR triggers a regulatory
mechanism which alleviates severity of TNBS-induced colitis in mice, implying that it is
worth further evaluation in prevention and treatment of IBDs.
PB  - Belgrade : Serbian Biochemical Society
PB  - Belgrade : Faculty of Chemistry
C3  - Serbian Biochemical Society Tenth Conference with international participation 24.09.2021. Kragujevac, Serbia “Biochemical Insights into Molecular Mechanisms”
T1  - Lactobacillus reuteri alleviate the severity of chemicallyinduced colitis in mice
EP  - 81
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_intor_868
ER  - 

@conference{
author = "Lukić, Ivana and Popović, Mina and Injac, Ninoslava and Kovačević, Ana and Miljković, Radmila and Stojanović, Marijana",
year = "2021",
abstract = "Inflammatory bowel diseases (IBDs) are associated with reduced diversity and decreased
contribution of probiotic bacteria within the comensal microbiota. A range of probiotic
bacteria, including the genera Lactobacillus, could be protective in IBDs due to their
capacity to trigger anti-inflammatory mechanisms 1. The aim of the study was to explore
immunomodulatory impact of probiotic bacteria. Lactobacillus reuteri (LR) and its
contribution to the alleviation of severity of a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-
induced colitis in mice. Colitis was induced in outbred Intor Swiss:Albino mice by a single
administration of TNBS dissolved in 50% ethanol (day 0). LR in phosphate buffer solution
(5x106 CFU/mL, p.o.) was given daily, 7 days prior and/or 7 days after day 0 (n=10 per
treatment). Mice subjected to the induction of colitis without LR treatment were referent.
A significant reduction in disease severity was noticed only in group treated by LR prior
and upon colitis induction. The alleviation of disease severity correlated with lessening of
local infiltration of leukocytes, a decrease in local inflammatory response
(myeloperoxidase activity, production of superoxide ions, IL-6, a nd T NFα), a nd a n
increase in local production of IL-10. Presented results show that LR triggers a regulatory
mechanism which alleviates severity of TNBS-induced colitis in mice, implying that it is
worth further evaluation in prevention and treatment of IBDs.",
publisher = "Belgrade : Serbian Biochemical Society, Belgrade : Faculty of Chemistry",
journal = "Serbian Biochemical Society Tenth Conference with international participation 24.09.2021. Kragujevac, Serbia “Biochemical Insights into Molecular Mechanisms”",
title = "Lactobacillus reuteri alleviate the severity of chemicallyinduced colitis in mice",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_intor_868"
}

Lukić, I., Popović, M., Injac, N., Kovačević, A., Miljković, R.,& Stojanović, M.. (2021). Lactobacillus reuteri alleviate the severity of chemicallyinduced colitis in mice. in Serbian Biochemical Society Tenth Conference with international participation 24.09.2021. Kragujevac, Serbia “Biochemical Insights into Molecular Mechanisms”
Belgrade : Serbian Biochemical Society., 81-81.
https://hdl.handle.net/21.15107/rcub_intor_868

Lukić I, Popović M, Injac N, Kovačević A, Miljković R, Stojanović M. Lactobacillus reuteri alleviate the severity of chemicallyinduced colitis in mice. in Serbian Biochemical Society Tenth Conference with international participation 24.09.2021. Kragujevac, Serbia “Biochemical Insights into Molecular Mechanisms”. 2021;:81-81.
https://hdl.handle.net/21.15107/rcub_intor_868 .

Lukić, Ivana, Popović, Mina, Injac, Ninoslava, Kovačević, Ana, Miljković, Radmila, Stojanović, Marijana, "Lactobacillus reuteri alleviate the severity of chemicallyinduced colitis in mice" in Serbian Biochemical Society Tenth Conference with international participation 24.09.2021. Kragujevac, Serbia “Biochemical Insights into Molecular Mechanisms” (2021):81-81,
https://hdl.handle.net/21.15107/rcub_intor_868 .