TY  - CONF
AU  - Pešić, V.
AU  - Stojić-Vukanić, Z.
AU  - Perišić, M.
AU  - Pilipović, Ivan
AU  - Radojević, K.
AU  - Kosec, Duško
AU  - Arsenović-Ranin, N.
AU  - Leposavić, Gordana
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/847
AB  - We examined influence of aging on: i) a1- AR expression on thymocytes and ii)
a1-AR mediated modulation of thymopoiesis. Using immunocytochemical and
flow cytometric analysis (FCA) of a1-AR expression on thymocytes from young
(2 m.) and old (18 m.) Wistar rats, an increase in a1-AR density on thymocytes
with advancing age was demonstrated. Age-associated plasticity of a1-AR
mediated tuning of T-cell development was assessed by FCA of thymocyte phenotypic profile following 14-day-long treatment with a1-AR blocker urapidil in
young and old rats. Urapidil augmented thymocyte number in rats of both
ages, but this increase reached statistical significance only in young rats.
Affecting generally the same thymocyte differentiational points (transition from
CD4+CD8+TCRab- to CD4+CD8+TCRablow stage, thymocyte selection and
lineage commitment), but with distinct efficiency, irrespective of age, urapidil
led to divergent changes in relative number of the most mature single positive
cells. Urapidil increased the percentage of CD4+CD8-TCRabhigh, but
decreased that of CD4-CD8+TCRabhigh cells. In old rats, differently from
young rats (showing increase in Thy-1 surface density), there were no changes
in Thy-1 expression (most likely due to a diminished central sympatholitic action
of urapidil), and presumably related efficiency of thymocyte selection processes,
suggesting an increased effectiveness of a1-AR mechanisms in old
rat thymus. To further support this hypothesis were findings demonstrating a
more pronounced urapidil-induced increase in relative number of thymocytes
with regulatory phenotype (CD4+CD25+RT6.1- and CD161+TCRab+) with
advancing age. In conclusion, changes in efficiency of modulatory thymic
a1-AR-mediated mechanisms may contribute to age-associated remodelling
of thymopoiesis.
PB  - The Japanese Society for Immunology
PB  - The Japan Society for Clinical Immunology
PB  - Science Council of Japan
PB  - International Union of Immunological Societies
C3  - 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan
T1  - Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats
EP  - iv 152
SP  - iv 151
SP  - PP-105-30
UR  - https://hdl.handle.net/21.15107/rcub_intor_847
ER  - 

@conference{
author = "Pešić, V. and Stojić-Vukanić, Z. and Perišić, M. and Pilipović, Ivan and Radojević, K. and Kosec, Duško and Arsenović-Ranin, N. and Leposavić, Gordana",
year = "2010",
abstract = "We examined influence of aging on: i) a1- AR expression on thymocytes and ii)
a1-AR mediated modulation of thymopoiesis. Using immunocytochemical and
flow cytometric analysis (FCA) of a1-AR expression on thymocytes from young
(2 m.) and old (18 m.) Wistar rats, an increase in a1-AR density on thymocytes
with advancing age was demonstrated. Age-associated plasticity of a1-AR
mediated tuning of T-cell development was assessed by FCA of thymocyte phenotypic profile following 14-day-long treatment with a1-AR blocker urapidil in
young and old rats. Urapidil augmented thymocyte number in rats of both
ages, but this increase reached statistical significance only in young rats.
Affecting generally the same thymocyte differentiational points (transition from
CD4+CD8+TCRab- to CD4+CD8+TCRablow stage, thymocyte selection and
lineage commitment), but with distinct efficiency, irrespective of age, urapidil
led to divergent changes in relative number of the most mature single positive
cells. Urapidil increased the percentage of CD4+CD8-TCRabhigh, but
decreased that of CD4-CD8+TCRabhigh cells. In old rats, differently from
young rats (showing increase in Thy-1 surface density), there were no changes
in Thy-1 expression (most likely due to a diminished central sympatholitic action
of urapidil), and presumably related efficiency of thymocyte selection processes,
suggesting an increased effectiveness of a1-AR mechanisms in old
rat thymus. To further support this hypothesis were findings demonstrating a
more pronounced urapidil-induced increase in relative number of thymocytes
with regulatory phenotype (CD4+CD25+RT6.1- and CD161+TCRab+) with
advancing age. In conclusion, changes in efficiency of modulatory thymic
a1-AR-mediated mechanisms may contribute to age-associated remodelling
of thymopoiesis.",
publisher = "The Japanese Society for Immunology, The Japan Society for Clinical Immunology, Science Council of Japan, International Union of Immunological Societies",
journal = "14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan",
title = "Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats",
pages = "iv 152-iv 151-PP-105-30",
url = "https://hdl.handle.net/21.15107/rcub_intor_847"
}

Pešić, V., Stojić-Vukanić, Z., Perišić, M., Pilipović, I., Radojević, K., Kosec, D., Arsenović-Ranin, N.,& Leposavić, G.. (2010). Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats. in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan
The Japanese Society for Immunology., iv 151-iv 152.
https://hdl.handle.net/21.15107/rcub_intor_847

Pešić V, Stojić-Vukanić Z, Perišić M, Pilipović I, Radojević K, Kosec D, Arsenović-Ranin N, Leposavić G. Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats. in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan. 2010;:iv 151-iv 152.
https://hdl.handle.net/21.15107/rcub_intor_847 .

Pešić, V., Stojić-Vukanić, Z., Perišić, M., Pilipović, Ivan, Radojević, K., Kosec, Duško, Arsenović-Ranin, N., Leposavić, Gordana, "Increased efficency of a1- adrenoceptor mediated thymopoesis in old rats" in 14th International Congress of Immunology, ICI 2010 Wrap-up Report, Immunology in the 21st Century: Defeating Infection, Autoimmunity, Allergy and Cancer, August 22-27, 2010 Kobe, Japan (2010):iv 151-iv 152,
https://hdl.handle.net/21.15107/rcub_intor_847 .