TY  - JOUR
AU  - Arsić, Bogdan
AU  - Gligić, Ana
AU  - Ristanović, Elizabeta
AU  - Lako, Branislav
AU  - Potkonjak, Aleksandar
AU  - Peruničić, Milan
AU  - Pavlović, Momčilo
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/403
AB  - Introduction Ehrlichiosis is a bacterial zoonosis transmitted by hematophagous arthropods - ticks. In humans, it occurs as monocytic, granulocytic, and ewingii ehrlichiosis. Pathological process is based on parasitic presence of Ehrlichia organisms within peripheral blood cells - monocytes and granulocytes. Case Outline Fifty-two year old patient was admitted to hospital due to high fever of over 40°C that lasted two days, accompanied with chills, muscle aches, malaise, loss of appetite, headache, confusion, breathing difficulties, and mild dry cough. The history suggested tick bite that occurred seven days before the onset of disease. Doxycycline was introduced and administered for 14 days, causing the disease to subside. Indirect immunofluorescence assay was used to analyze three serum samples obtained from this patient for Ehrlichia chaffeensis antibodies, and peripheral blood smear was evaluated for the presence of Ehrlichia and Ehrlichia aggregation into morulae. Conclusion Ehrlichiosis should be considered in each case where there is a history of tick bite together with the clinical picture (high fever, chills, muscle aches, headache, generalized weakness and malaise, and possible maculopapular rash). The presence of Ehrlichia chaffeensis antibodies was confirmed in a patient with the history of tick bite, appropriate clinical picture and indirect immunofluorescence assay. This confirmed the presence of human monocytotropic ehrlichiosis, a disease that is uncommonly identified in our country.
AB  - Uvod Erlihioza je bakterijska zoonoza koja se prenosi hematofagnim artropodama - krpeljima. Kod ljudi se javlja kao monocitna, granulocitna i ewingii erlihioza. Patološki proces je posledica unutar ćelijskog parazitiranja erlihije u monocitima i granulocitima periferne krvi. Prikaz bolesnika Bolesnik star 52 godine primljen je na odeljenje zbog visoke dvodnevne febrilnosti (preko 40°C) koja je praćena drhtavicom, bolovima u mišićima, malaksalošću, gubitkom apetita, glavoboljom, konfuznošću, otežanim disanjem i oskudnim suvim kašljem. U anamnezi je dobijen podatak o ujedu krpelja sedam dana pre prijema. Laboratorijski nalazi su ukazali na trombocitopeniju, leukopeniju, anemiju i povećanje aktivnosti transaminaza u serumu. Bolesnik je lečen doksiciklinom 14 dana, nakon čega su se tegobe povukle. Metodom indirektne imunofluorescencije analizirana su tri uzorka seruma ovog bolesnika na prisustvo antitela na bakteriju Ehrlichia chaffeensis i pregledan je uzorak razmaza periferne krvi na prisustvo erlihija i konglomerata erlihije u morule, koje predstavljaju citoplazmatske vakuole. Zaključak Kod bolesnika s podatkom o ubodu krpelja, odgovarajućom kliničkom slikom i serološkim testom indirektne imunofluorescencije dokazana su antitela za bakteriju Ehrlichia chaffeensis, što ukazuje na humanu monocitotropnu erlihiozu, bolest koja se kod nas retko dokazuje. Na erlihiozu treba misliti kada uz kliničku sliku (visoka febrilnost, groznica, bolovi u mišićima, glavobolja, opšta slabost i malaksalost, eventualno makulopapulozna ospa) postoji podatak o ubodu krpelja.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - A case of human monocytic ehrlichiosis in Serbia
T1  - Slučaj humane monocitne erlihioze u Srbiji
EP  - 82
IS  - 1-2
SP  - 79
VL  - 142
DO  - 10.2298/SARH1402079A
ER  - 

@article{
author = "Arsić, Bogdan and Gligić, Ana and Ristanović, Elizabeta and Lako, Branislav and Potkonjak, Aleksandar and Peruničić, Milan and Pavlović, Momčilo",
year = "2014",
abstract = "Introduction Ehrlichiosis is a bacterial zoonosis transmitted by hematophagous arthropods - ticks. In humans, it occurs as monocytic, granulocytic, and ewingii ehrlichiosis. Pathological process is based on parasitic presence of Ehrlichia organisms within peripheral blood cells - monocytes and granulocytes. Case Outline Fifty-two year old patient was admitted to hospital due to high fever of over 40°C that lasted two days, accompanied with chills, muscle aches, malaise, loss of appetite, headache, confusion, breathing difficulties, and mild dry cough. The history suggested tick bite that occurred seven days before the onset of disease. Doxycycline was introduced and administered for 14 days, causing the disease to subside. Indirect immunofluorescence assay was used to analyze three serum samples obtained from this patient for Ehrlichia chaffeensis antibodies, and peripheral blood smear was evaluated for the presence of Ehrlichia and Ehrlichia aggregation into morulae. Conclusion Ehrlichiosis should be considered in each case where there is a history of tick bite together with the clinical picture (high fever, chills, muscle aches, headache, generalized weakness and malaise, and possible maculopapular rash). The presence of Ehrlichia chaffeensis antibodies was confirmed in a patient with the history of tick bite, appropriate clinical picture and indirect immunofluorescence assay. This confirmed the presence of human monocytotropic ehrlichiosis, a disease that is uncommonly identified in our country., Uvod Erlihioza je bakterijska zoonoza koja se prenosi hematofagnim artropodama - krpeljima. Kod ljudi se javlja kao monocitna, granulocitna i ewingii erlihioza. Patološki proces je posledica unutar ćelijskog parazitiranja erlihije u monocitima i granulocitima periferne krvi. Prikaz bolesnika Bolesnik star 52 godine primljen je na odeljenje zbog visoke dvodnevne febrilnosti (preko 40°C) koja je praćena drhtavicom, bolovima u mišićima, malaksalošću, gubitkom apetita, glavoboljom, konfuznošću, otežanim disanjem i oskudnim suvim kašljem. U anamnezi je dobijen podatak o ujedu krpelja sedam dana pre prijema. Laboratorijski nalazi su ukazali na trombocitopeniju, leukopeniju, anemiju i povećanje aktivnosti transaminaza u serumu. Bolesnik je lečen doksiciklinom 14 dana, nakon čega su se tegobe povukle. Metodom indirektne imunofluorescencije analizirana su tri uzorka seruma ovog bolesnika na prisustvo antitela na bakteriju Ehrlichia chaffeensis i pregledan je uzorak razmaza periferne krvi na prisustvo erlihija i konglomerata erlihije u morule, koje predstavljaju citoplazmatske vakuole. Zaključak Kod bolesnika s podatkom o ubodu krpelja, odgovarajućom kliničkom slikom i serološkim testom indirektne imunofluorescencije dokazana su antitela za bakteriju Ehrlichia chaffeensis, što ukazuje na humanu monocitotropnu erlihiozu, bolest koja se kod nas retko dokazuje. Na erlihiozu treba misliti kada uz kliničku sliku (visoka febrilnost, groznica, bolovi u mišićima, glavobolja, opšta slabost i malaksalost, eventualno makulopapulozna ospa) postoji podatak o ubodu krpelja.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "A case of human monocytic ehrlichiosis in Serbia, Slučaj humane monocitne erlihioze u Srbiji",
pages = "82-79",
number = "1-2",
volume = "142",
doi = "10.2298/SARH1402079A"
}

Arsić, B., Gligić, A., Ristanović, E., Lako, B., Potkonjak, A., Peruničić, M.,& Pavlović, M.. (2014). A case of human monocytic ehrlichiosis in Serbia. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(1-2), 79-82.
https://doi.org/10.2298/SARH1402079A

Arsić B, Gligić A, Ristanović E, Lako B, Potkonjak A, Peruničić M, Pavlović M. A case of human monocytic ehrlichiosis in Serbia. in Srpski arhiv za celokupno lekarstvo. 2014;142(1-2):79-82.
doi:10.2298/SARH1402079A .

Arsić, Bogdan, Gligić, Ana, Ristanović, Elizabeta, Lako, Branislav, Potkonjak, Aleksandar, Peruničić, Milan, Pavlović, Momčilo, "A case of human monocytic ehrlichiosis in Serbia" in Srpski arhiv za celokupno lekarstvo, 142, no. 1-2 (2014):79-82,
https://doi.org/10.2298/SARH1402079A . .

TY  - JOUR
AU  - Samardžić, Svetomir
AU  - Marinković, Tatjana
AU  - Marinković, Dragan
AU  - Đuricić, Bosiljka
AU  - Ristanović, Elizabeta
AU  - Simović, Tatjana
AU  - Lako, Branislav
AU  - Vukov, Biljana
AU  - Božović, Bojana
AU  - Gligić, Ana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/258
AB  - We assayed the presence of antibodies specific for Rickettsia typhi, R. akari, and R. conorii in sera of persons from several localities in Serbia with different geographic, climatic, and lifestyle characteristics. Sera from 140 patients with unclear clinical symptoms and 273 healthy persons were tested for the presence of rickettsiae-specific antibodies by indirect immunofluorescence assay. In this study, for the first time we detected the presence of rickettsiae from the spotted fever group in Serbia. We detected the presence of antibodies against R. conorii in the samples from all tested localities. The proportion of positive cases was low in the plain agricultural areas but reached up to 23% in the mountain areas. We also observed a significant number of cases positive for antibodies against R. akari. Antibodies specific for the antigens of R. typhi were detected in only 2 samples from the municipality of Pec (Kosovo region). These findings contribute to the prevalence of Rickettsia species in Southeast Europe. Our study also revealed a dramatic lack of awareness of rickettsioses among medical personnel and pointed to the need for urgent measures that would help improve the current situation in the region.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Vector-Borne and Zoonotic Diseases
T1  - Prevalence of antibodies to Rickettsiae in different regions of Serbia
EP  - 224
IS  - 2
SP  - 219
VL  - 8
DO  - 10.1089/vbz.2007.0122
ER  - 

@article{
author = "Samardžić, Svetomir and Marinković, Tatjana and Marinković, Dragan and Đuricić, Bosiljka and Ristanović, Elizabeta and Simović, Tatjana and Lako, Branislav and Vukov, Biljana and Božović, Bojana and Gligić, Ana",
year = "2008",
abstract = "We assayed the presence of antibodies specific for Rickettsia typhi, R. akari, and R. conorii in sera of persons from several localities in Serbia with different geographic, climatic, and lifestyle characteristics. Sera from 140 patients with unclear clinical symptoms and 273 healthy persons were tested for the presence of rickettsiae-specific antibodies by indirect immunofluorescence assay. In this study, for the first time we detected the presence of rickettsiae from the spotted fever group in Serbia. We detected the presence of antibodies against R. conorii in the samples from all tested localities. The proportion of positive cases was low in the plain agricultural areas but reached up to 23% in the mountain areas. We also observed a significant number of cases positive for antibodies against R. akari. Antibodies specific for the antigens of R. typhi were detected in only 2 samples from the municipality of Pec (Kosovo region). These findings contribute to the prevalence of Rickettsia species in Southeast Europe. Our study also revealed a dramatic lack of awareness of rickettsioses among medical personnel and pointed to the need for urgent measures that would help improve the current situation in the region.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Vector-Borne and Zoonotic Diseases",
title = "Prevalence of antibodies to Rickettsiae in different regions of Serbia",
pages = "224-219",
number = "2",
volume = "8",
doi = "10.1089/vbz.2007.0122"
}

Samardžić, S., Marinković, T., Marinković, D., Đuricić, B., Ristanović, E., Simović, T., Lako, B., Vukov, B., Božović, B.,& Gligić, A.. (2008). Prevalence of antibodies to Rickettsiae in different regions of Serbia. in Vector-Borne and Zoonotic Diseases
Mary Ann Liebert, Inc, New Rochelle., 8(2), 219-224.
https://doi.org/10.1089/vbz.2007.0122

Samardžić S, Marinković T, Marinković D, Đuricić B, Ristanović E, Simović T, Lako B, Vukov B, Božović B, Gligić A. Prevalence of antibodies to Rickettsiae in different regions of Serbia. in Vector-Borne and Zoonotic Diseases. 2008;8(2):219-224.
doi:10.1089/vbz.2007.0122 .

Samardžić, Svetomir, Marinković, Tatjana, Marinković, Dragan, Đuricić, Bosiljka, Ristanović, Elizabeta, Simović, Tatjana, Lako, Branislav, Vukov, Biljana, Božović, Bojana, Gligić, Ana, "Prevalence of antibodies to Rickettsiae in different regions of Serbia" in Vector-Borne and Zoonotic Diseases, 8, no. 2 (2008):219-224,
https://doi.org/10.1089/vbz.2007.0122 . .

TY  - JOUR
AU  - Uzelac-Keserović, B.
AU  - Spasić, P.
AU  - Bojanić, N.
AU  - Dimitrijević, J.
AU  - Lako, Branislav
AU  - Lepšanović, Z.
AU  - Kuljić-Kapulica, N.
AU  - Vasić, D.
AU  - Apostolov, K.
PY  - 1999
UR  - http://intor.torlakinstitut.com/handle/123456789/109
AB  - Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33°C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis.
PB  - S. Karger AG
T2  - Nephron
T1  - Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients
EP  - 145
IS  - 2
SP  - 141
VL  - 81
DO  - 10.1159/000045269
ER  - 

@article{
author = "Uzelac-Keserović, B. and Spasić, P. and Bojanić, N. and Dimitrijević, J. and Lako, Branislav and Lepšanović, Z. and Kuljić-Kapulica, N. and Vasić, D. and Apostolov, K.",
year = "1999",
abstract = "Endemic Balkan nephropathy (EBN) is a kidney disease of unknown etiology limited to Bulgaria, Rumania and former Yugoslavia. Primary kidney tissue cultures were established as explants from tissue obtained at operations from 5 EBN patients with urinary tract tumors. Four out of the five biopsy specimens on extended culture incubation at 33°C yielded a coronavirus virus (EBNV) which was cytopathogenic for human fibroblast and Vero cells. In cells inoculated with EBNV, cytoplasmic immunofluorescence was found using antisera for human coronaviruses OC43 and 229E as well as the porcine transmissible gastroenteric virus and avian (chicken) bronchitis virus. In neutralization tests, EBNV failed to react with antisera to these viruses. Using hyperimmune serum raised with EBNV, positive cytoplasmic immunofluorescence was seen with cells infected with OC43, 229E, TGV and significantly with the kidney tissue of the biopsy specimens from the EBN patients. A screen for neutralizing antibody using the EBN virus revealed that 87.2% of EBN patients on dialysis were positive, also 74% of people from an endemic area were also positive, while only 13.5% from outside were positive. It is suggested that a coronavirus is involved in the etiology of the disease and that humans are an incidental host of a coronavirus zoonosis.",
publisher = "S. Karger AG",
journal = "Nephron",
title = "Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients",
pages = "145-141",
number = "2",
volume = "81",
doi = "10.1159/000045269"
}

Uzelac-Keserović, B., Spasić, P., Bojanić, N., Dimitrijević, J., Lako, B., Lepšanović, Z., Kuljić-Kapulica, N., Vasić, D.,& Apostolov, K.. (1999). Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients. in Nephron
S. Karger AG., 81(2), 141-145.
https://doi.org/10.1159/000045269

Uzelac-Keserović B, Spasić P, Bojanić N, Dimitrijević J, Lako B, Lepšanović Z, Kuljić-Kapulica N, Vasić D, Apostolov K. Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients. in Nephron. 1999;81(2):141-145.
doi:10.1159/000045269 .

Uzelac-Keserović, B., Spasić, P., Bojanić, N., Dimitrijević, J., Lako, Branislav, Lepšanović, Z., Kuljić-Kapulica, N., Vasić, D., Apostolov, K., "Isolation of a coronavirus from kidney biopsies of endemic Balkan nephropathy patients" in Nephron, 81, no. 2 (1999):141-145,
https://doi.org/10.1159/000045269 . .