TY  - CONF
AU  - Vujinović, I.
AU  - Pilipović, Ivan
AU  - Petrović, R.
AU  - Stojić−Vukanić, Z.
AU  - Arsenović−Ranin, N.
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/852
AB  - Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.
C3  - Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands
T1  - Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes
SP  - P.A5.06.07
UR  - https://hdl.handle.net/21.15107/rcub_intor_852
ER  - 

@conference{
author = "Vujinović, I. and Pilipović, Ivan and Petrović, R. and Stojić−Vukanić, Z. and Arsenović−Ranin, N. and Leposavić, Gordana",
year = "2018",
abstract = "Introduction: Catecholamines are implicated in development of multiple sclerosis and EAE in Dark Agouti (DA) rats. To enlighten their β−adrenoceptor−mediated immunomodulatory action, DA rats of both sexes immunized for EAE were subjected to seven−day−long treatment with propranolol (β−adrenoceptor blocker) starting at the day of immunization.
Methods: The migration of antigen presenting cells (APCs) into the draining lymph nodes (dLNs) was examined using CFSE−based assay. Frequency of activated CD4+ T cells, their proliferation and frequency of IL−17−producing CD4+ T (Th17) cells in dLNs, and their infiltration into spinal cord, were analyzed using flow cytometry. Propranolol effects on CD4+ cell proliferation and Th17 cell polarization, and IL−2 and Th17 polarizing cytokine and chemokine expression in dLNs/ dLN cell cultures were examined using flow cytometry and ELISA/qRT−PCR. Results: Irrespective of sex, propranolol reduced the incidence and postponed clinical EAE onset by impairing migration of antigen−carrying APCs into the dLNs (due to diminished dLN CCL19/21 expression). Consequently, propranolol diminished CD4+ T−cell activation/proliferation, and Th17 cell number in dLNs and spinal cord. To corroborate these findings, propranolol exerted stimulatory effects on CD4+ cell proliferation (through stimulation of IL−2 secretion) and Th17 cell differentiation (reflecting enhanced Th17 polarizing cytokine production) in dLN cell cultures. Conclusions: Irrespective of sex, the stimulatory effects of propranolol on dLN CD4+ T lymphocyte proliferation and activated/ matured APC Th17 polarizing capacity were insufficient to overcome its inhibitory influence on APC migration, so propranolol impaired Th17 generation in dLNs of EAE rats, and postponed EAE development.",
journal = "Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands",
title = "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes",
pages = "P.A5.06.07",
url = "https://hdl.handle.net/21.15107/rcub_intor_852"
}

Vujinović, I., Pilipović, I., Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N.,& Leposavić, G.. (2018). Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands, P.A5.06.07.
https://hdl.handle.net/21.15107/rcub_intor_852

Vujinović I, Pilipović I, Petrović R, Stojić−Vukanić Z, Arsenović−Ranin N, Leposavić G. Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes. in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands. 2018;:P.A5.06.07.
https://hdl.handle.net/21.15107/rcub_intor_852 .

Vujinović, I., Pilipović, Ivan, Petrović, R., Stojić−Vukanić, Z., Arsenović−Ranin, N., Leposavić, Gordana, "Propranolol influences EAE development by impairing antigen presenting cell migration into the draining lymph nodes" in Abstracts of the 5th European Congress of Immunology - ECI 2018 - Amsterdam, The Netherlands (2018):P.A5.06.07,
https://hdl.handle.net/21.15107/rcub_intor_852 .

TY  - CONF
AU  - Petrović, R.
AU  - Milovanović, R.
PY  - 1990
UR  - http://intor.torlakinstitut.com/handle/123456789/42
C3  - Giornale di Malattie Infettive e Parassitarie
T1  - Epidemic aspects of the immunization against whooping cough in Serbia
EP  - 533
IS  - 7
SP  - 531
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_intor_42
ER  - 

@conference{
author = "Petrović, R. and Milovanović, R.",
year = "1990",
journal = "Giornale di Malattie Infettive e Parassitarie",
title = "Epidemic aspects of the immunization against whooping cough in Serbia",
pages = "533-531",
number = "7",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_intor_42"
}

Petrović, R.,& Milovanović, R.. (1990). Epidemic aspects of the immunization against whooping cough in Serbia. in Giornale di Malattie Infettive e Parassitarie, 42(7), 531-533.
https://hdl.handle.net/21.15107/rcub_intor_42

Petrović R, Milovanović R. Epidemic aspects of the immunization against whooping cough in Serbia. in Giornale di Malattie Infettive e Parassitarie. 1990;42(7):531-533.
https://hdl.handle.net/21.15107/rcub_intor_42 .

Petrović, R., Milovanović, R., "Epidemic aspects of the immunization against whooping cough in Serbia" in Giornale di Malattie Infettive e Parassitarie, 42, no. 7 (1990):531-533,
https://hdl.handle.net/21.15107/rcub_intor_42 .

TY  - JOUR
AU  - Petrović, R.
AU  - Gligić, Ana
AU  - Petrović, N.
PY  - 1974
UR  - http://intor.torlakinstitut.com/handle/123456789/12
PB  - Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad
T2  - Medicinski pregled
T1  - Late local changes after smallpox vaccination (Serbocroatian)
EP  - 33
IS  - 1-2
SP  - 29
VL  - 27
UR  - https://hdl.handle.net/21.15107/rcub_intor_12
ER  - 

@article{
author = "Petrović, R. and Gligić, Ana and Petrović, N.",
year = "1974",
publisher = "Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad",
journal = "Medicinski pregled",
title = "Late local changes after smallpox vaccination (Serbocroatian)",
pages = "33-29",
number = "1-2",
volume = "27",
url = "https://hdl.handle.net/21.15107/rcub_intor_12"
}

Petrović, R., Gligić, A.,& Petrović, N.. (1974). Late local changes after smallpox vaccination (Serbocroatian). in Medicinski pregled
Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad., 27(1-2), 29-33.
https://hdl.handle.net/21.15107/rcub_intor_12

Petrović R, Gligić A, Petrović N. Late local changes after smallpox vaccination (Serbocroatian). in Medicinski pregled. 1974;27(1-2):29-33.
https://hdl.handle.net/21.15107/rcub_intor_12 .

Petrović, R., Gligić, Ana, Petrović, N., "Late local changes after smallpox vaccination (Serbocroatian)" in Medicinski pregled, 27, no. 1-2 (1974):29-33,
https://hdl.handle.net/21.15107/rcub_intor_12 .