TY  - JOUR
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Fira, Đorđe
AU  - Miladinov, Nataša
AU  - Strahinić, Ivana
AU  - Gajić, Olivera
AU  - Arsenijević, Slavica
AU  - Spasojević, Irena
AU  - Vukasinović, Maja
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/793
AB  - Autohtoni sojevi bakterija mlečne kiseline (BMK) su izolovane iz fermentisanih mlečnih proizvoda rađenih u domaćoj radinosti. Fermentisani mlečni proizvodi su sakupljani sa specifičnih ekoloških lokaliteta kao što su visoke planine (iznad 1200 m nadmorske visine), planinske visoravni, rečne doline, ostrva, obala Jadranskog mora, itd. Analiza kolekcije prirodnih izolata BMK je pokazala da izolati proizvode proteinaze, bakteriocine i egzopolisaharide. Pokazano je, takođe, da neki izolati laktokoka i laktobacila proizvode istovremeno dva različita bakteriocina. Pored toga određeni broj izolata proizvodi i proteinaze i bakteriocine. Nađeno je da prirodni izolati sintetišu specifične proteinaze. Analiza organizacije prt gena u prirodnim izolatima BMK je pokazala da četiri laktobacila poseduju organizaciju prt gena koja se razlikuje od do sada opisanih. Izučavanje regulacije gena koji kodiraju proteinaze, bakteriocine ili egzopolisaharide može olakšati konstrukciju specifičnih starter kultura za proizvodnju autohtonih fermentisanih mlečnih proizvoda, tj. fermentisanih proizvoda sa geografskim poreklom.
AB  - Autochthonous strains of lactic acid bacteria (LAB) were isolated from homemade fermented milk products. These products were collected from specific ecological localities such as high mountains (above 1200 m above sea level) mountain plateaus, river valleys, islands, Adriatic coast, etc. Analysis of LAB from the collection of natural isolates revealed that they produce proteinases, bacteriocins and exopolysaccharides. It was also shown that some isolates of lactococci and lactobacilli produce two bacteriocins simultaneously. According to their antimicrobial and biochemical properties most of the analyzed bacteriocins in natural isolates of lactococci were class 11 bacteriocins. In addition some isolates produced both proteinase and bacteriocin. Biosynthesis of specific proteinases was detected in natural isolates. Analysis of the prt gene organization among natural isolates of LAB showed that four isolates of lactobacilli exhibited prt genes organization different from those described so far. Elucidation of the regulation of the genes encoding proteinases, bacteriocins and exopolysaccharides could facilitate the construction of specific starter cultures for production of autochthonous fermented milk products, i.e. fermented products with a geographical origin.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Molekularna genetika autohtonih bakterija mlečne kiseline
T1  - Molecular genetics of autochthonous lactic acid bacteria
EP  - 127
IS  - 2
SP  - 115
VL  - 32
UR  - https://hdl.handle.net/21.15107/rcub_intor_793
ER  - 

@article{
author = "Topisirović, Ljubiša and Kojić, Milan and Fira, Đorđe and Miladinov, Nataša and Strahinić, Ivana and Gajić, Olivera and Arsenijević, Slavica and Spasojević, Irena and Vukasinović, Maja",
year = "2000",
abstract = "Autohtoni sojevi bakterija mlečne kiseline (BMK) su izolovane iz fermentisanih mlečnih proizvoda rađenih u domaćoj radinosti. Fermentisani mlečni proizvodi su sakupljani sa specifičnih ekoloških lokaliteta kao što su visoke planine (iznad 1200 m nadmorske visine), planinske visoravni, rečne doline, ostrva, obala Jadranskog mora, itd. Analiza kolekcije prirodnih izolata BMK je pokazala da izolati proizvode proteinaze, bakteriocine i egzopolisaharide. Pokazano je, takođe, da neki izolati laktokoka i laktobacila proizvode istovremeno dva različita bakteriocina. Pored toga određeni broj izolata proizvodi i proteinaze i bakteriocine. Nađeno je da prirodni izolati sintetišu specifične proteinaze. Analiza organizacije prt gena u prirodnim izolatima BMK je pokazala da četiri laktobacila poseduju organizaciju prt gena koja se razlikuje od do sada opisanih. Izučavanje regulacije gena koji kodiraju proteinaze, bakteriocine ili egzopolisaharide može olakšati konstrukciju specifičnih starter kultura za proizvodnju autohtonih fermentisanih mlečnih proizvoda, tj. fermentisanih proizvoda sa geografskim poreklom., Autochthonous strains of lactic acid bacteria (LAB) were isolated from homemade fermented milk products. These products were collected from specific ecological localities such as high mountains (above 1200 m above sea level) mountain plateaus, river valleys, islands, Adriatic coast, etc. Analysis of LAB from the collection of natural isolates revealed that they produce proteinases, bacteriocins and exopolysaccharides. It was also shown that some isolates of lactococci and lactobacilli produce two bacteriocins simultaneously. According to their antimicrobial and biochemical properties most of the analyzed bacteriocins in natural isolates of lactococci were class 11 bacteriocins. In addition some isolates produced both proteinase and bacteriocin. Biosynthesis of specific proteinases was detected in natural isolates. Analysis of the prt gene organization among natural isolates of LAB showed that four isolates of lactobacilli exhibited prt genes organization different from those described so far. Elucidation of the regulation of the genes encoding proteinases, bacteriocins and exopolysaccharides could facilitate the construction of specific starter cultures for production of autochthonous fermented milk products, i.e. fermented products with a geographical origin.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Molekularna genetika autohtonih bakterija mlečne kiseline, Molecular genetics of autochthonous lactic acid bacteria",
pages = "127-115",
number = "2",
volume = "32",
url = "https://hdl.handle.net/21.15107/rcub_intor_793"
}

Topisirović, L., Kojić, M., Fira, Đ., Miladinov, N., Strahinić, I., Gajić, O., Arsenijević, S., Spasojević, I.,& Vukasinović, M.. (2000). Molekularna genetika autohtonih bakterija mlečne kiseline. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 32(2), 115-127.
https://hdl.handle.net/21.15107/rcub_intor_793

Topisirović L, Kojić M, Fira Đ, Miladinov N, Strahinić I, Gajić O, Arsenijević S, Spasojević I, Vukasinović M. Molekularna genetika autohtonih bakterija mlečne kiseline. in Genetika-Belgrade. 2000;32(2):115-127.
https://hdl.handle.net/21.15107/rcub_intor_793 .

Topisirović, Ljubiša, Kojić, Milan, Fira, Đorđe, Miladinov, Nataša, Strahinić, Ivana, Gajić, Olivera, Arsenijević, Slavica, Spasojević, Irena, Vukasinović, Maja, "Molekularna genetika autohtonih bakterija mlečne kiseline" in Genetika-Belgrade, 32, no. 2 (2000):115-127,
https://hdl.handle.net/21.15107/rcub_intor_793 .