TY  - CONF
AU  - Bufan, B.
AU  - Arsenović-Ranin, N.
AU  - Dimitrijević, M.
AU  - Nacka-Aleksić, M.
AU  - Kosec, Duško
AU  - Pilipović, Ivan
AU  - Stojanović, M.
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/850
AB  - Introduction: Considering sex bias in rheumatoid arthritis prevalence, influence of biological sex on the disease development in Dark Agouti rat collagen II (CII)−induced arthritis (CIA) model of the human disease was examined. Methods: Sex bias in CD4+ T cell responses in inguinal (draining the site of immunization in preclinical CIA) and popliteal (draining inflamed joints at the peak of CIA) lymph nodes (LNs) and mechanisms controlling their development were examined using flow cytometry and/or ELISA/qRT−PCR. Results: In both inguinal and popliteal LNs greater number of CD4+CD25+Foxp3− cells, presumably activated effector T cells, was found in females compared with males, and they exhibited greater CII−specific proliferation. Consistently, more IL−17+, IFN−γ+ and IL−17+IFN−γ+ T cells were retrieved from both inguinal and popliteal female rat LNs. Moreover, more GM−CSF+ and IL−17+IFN−γ+GM−CSF+ T cells were retrieved from female compared with male rat popliteal LNs. On the other hand, lower frequency of PD−1+ cells among CD4+CD25+Foxp3+ regulatory T cells (Tregs) from female popliteal and inguinal LNs suggested lower suppressive capacity of their Tregs. Additionally, from female rat popliteal LNs fewer Tregs were recovered. Furthermore, the number of regulatory LN B10 cells was lower in females. Moreover, compared with males, in females was shifted LN INF−γ+/IL−4+ T−cell ratio towards the former, and accordingly serum CII−specific IgG2a/IgG1 antibody ratio was shifted towards pathogenic IgG2a antibodies. Conclusion: The study suggests that a less efficient control of (auto)immune Th1/Th17 cell responses during CIA development contributes to sex bias in the susceptibility to CIA.
C3  - 5th European Congress of Immunology, Septembar 2-5, Abstract Book
T1  - Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis
SP  - P.C1.01.02
UR  - https://hdl.handle.net/21.15107/rcub_intor_850
ER  - 

@conference{
author = "Bufan, B. and Arsenović-Ranin, N. and Dimitrijević, M. and Nacka-Aleksić, M. and Kosec, Duško and Pilipović, Ivan and Stojanović, M. and Leposavić, Gordana",
year = "2018",
abstract = "Introduction: Considering sex bias in rheumatoid arthritis prevalence, influence of biological sex on the disease development in Dark Agouti rat collagen II (CII)−induced arthritis (CIA) model of the human disease was examined. Methods: Sex bias in CD4+ T cell responses in inguinal (draining the site of immunization in preclinical CIA) and popliteal (draining inflamed joints at the peak of CIA) lymph nodes (LNs) and mechanisms controlling their development were examined using flow cytometry and/or ELISA/qRT−PCR. Results: In both inguinal and popliteal LNs greater number of CD4+CD25+Foxp3− cells, presumably activated effector T cells, was found in females compared with males, and they exhibited greater CII−specific proliferation. Consistently, more IL−17+, IFN−γ+ and IL−17+IFN−γ+ T cells were retrieved from both inguinal and popliteal female rat LNs. Moreover, more GM−CSF+ and IL−17+IFN−γ+GM−CSF+ T cells were retrieved from female compared with male rat popliteal LNs. On the other hand, lower frequency of PD−1+ cells among CD4+CD25+Foxp3+ regulatory T cells (Tregs) from female popliteal and inguinal LNs suggested lower suppressive capacity of their Tregs. Additionally, from female rat popliteal LNs fewer Tregs were recovered. Furthermore, the number of regulatory LN B10 cells was lower in females. Moreover, compared with males, in females was shifted LN INF−γ+/IL−4+ T−cell ratio towards the former, and accordingly serum CII−specific IgG2a/IgG1 antibody ratio was shifted towards pathogenic IgG2a antibodies. Conclusion: The study suggests that a less efficient control of (auto)immune Th1/Th17 cell responses during CIA development contributes to sex bias in the susceptibility to CIA.",
journal = "5th European Congress of Immunology, Septembar 2-5, Abstract Book",
title = "Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis",
pages = "P.C1.01.02",
url = "https://hdl.handle.net/21.15107/rcub_intor_850"
}

Bufan, B., Arsenović-Ranin, N., Dimitrijević, M., Nacka-Aleksić, M., Kosec, D., Pilipović, I., Stojanović, M.,& Leposavić, G.. (2018). Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis. in 5th European Congress of Immunology, Septembar 2-5, Abstract Book, P.C1.01.02.
https://hdl.handle.net/21.15107/rcub_intor_850

Bufan B, Arsenović-Ranin N, Dimitrijević M, Nacka-Aleksić M, Kosec D, Pilipović I, Stojanović M, Leposavić G. Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis. in 5th European Congress of Immunology, Septembar 2-5, Abstract Book. 2018;:P.C1.01.02.
https://hdl.handle.net/21.15107/rcub_intor_850 .

Bufan, B., Arsenović-Ranin, N., Dimitrijević, M., Nacka-Aleksić, M., Kosec, Duško, Pilipović, Ivan, Stojanović, M., Leposavić, Gordana, "Sexual dimorphism in mechanisms controlling development of CD4+ T cell response in collagen-induced arthritis" in 5th European Congress of Immunology, Septembar 2-5, Abstract Book (2018):P.C1.01.02,
https://hdl.handle.net/21.15107/rcub_intor_850 .