TY  - CONF
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Petrović, Raisa
AU  - Vujnović, I.
AU  - Dimitrijević, Mirjana
AU  - Vujić, V.
AU  - Stanojević, Stanislava
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/671
AB  - The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of either LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected.
C3  - 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book
T1  - Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4
EP  - 341
SP  - 341
SP  - P.C.12.06
UR  - https://hdl.handle.net/21.15107/rcub_intor_671
ER  - 

@conference{
author = "Blagojević, Veljko and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, I. and Dimitrijević, Mirjana and Vujić, V. and Stanojević, Stanislava and Leposavić, Gordana",
year = "2015",
abstract = "The influence of aging on phagocytic, antigen presenting and secretory capacity of resident Albino Oxford rat peritoneal macrophages cultured for 24 hours in medium alone and in the presence of either LPS or IL-4 was examined. The frequencies of CD68+, CD169+ and CCR7+ cells were comparable among fresh peritoneal cells from young (3-months-old) and aged (i8-months-old) rats, whereas those of CD163+, MHCII+ and CD86+ cells were lower within the same cell population in aged relative to young rats. Congruent with the latter findings, aged rat macrophages cultured in medium alone exhibited impaired allostimulatory properties. Additionally, their zymosan phagocytizing ability was reduced. Differently from young rat macrophages, aged rat macrophages changed neither allostimulatory nor zymosan phagocytizing ability upon in vitro treatment with either LPS or IL-4. In the presence of LPS, NO production comparably increased in macrophages from young and aged rats. However, in the presence of either LPS or IL-4 urea production increased only in macrophages from aged rats. Differently from LPS, which increased the prototypic proinflamrnatory cytokine production in macrophages from rats of both ages, the diminishing effect of IL-4 on their production was evident only in macrophages from young rats. Collectively, our results suggest that aging, besides diminishing influence on macrophage allostimulatory and zymosan phagocytizing ability, may impair their responsiveness to IL-4 in vitro. Consequently, an altered efficacy of inflammatory/immune responses in aged rats may be expected.",
journal = "4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book",
title = "Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4",
pages = "341-341-P.C.12.06",
url = "https://hdl.handle.net/21.15107/rcub_intor_671"
}

Blagojević, V., Ćuruvija, I., Petrović, R., Vujnović, I., Dimitrijević, M., Vujić, V., Stanojević, S.,& Leposavić, G.. (2015). Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4. in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book, 341-341.
https://hdl.handle.net/21.15107/rcub_intor_671

Blagojević V, Ćuruvija I, Petrović R, Vujnović I, Dimitrijević M, Vujić V, Stanojević S, Leposavić G. Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4. in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book. 2015;:341-341.
https://hdl.handle.net/21.15107/rcub_intor_671 .

Blagojević, Veljko, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, I., Dimitrijević, Mirjana, Vujić, V., Stanojević, Stanislava, Leposavić, Gordana, "Aged rat macrophages exhibit ipaired response to in vitro stimulation with IL-4" in 4th European Congress of Immunology, Vienna 2015, September 6-9, abstract book (2015):341-341,
https://hdl.handle.net/21.15107/rcub_intor_671 .

TY  - JOUR
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Lazarević-Macanović, Miriana
AU  - Dimitrijević, Mirjana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/229
AB  - There is extensive evidence for the critical role of reactive oxygen species (ROS) and nitric oxide (NO) produced by phagocytes in development of inflammatory processes and pathogenesis of numerous diseases, including rheumatoid arthritis (RA). Apart from their function as mediators of inflammation and tissue damage, recent research supports their role as signaling and regulatory molecules. In the present study we have investigated the production of ROS and NO over the course of adjuvant arthritis (AA) and oil-induced arthritis (OIA), by resident peritoneal macrophages of two rat strains: Dark Agouti (DA), susceptible, and Albino Oxford (AO), resistant to induction of AA and OIA. We have compared levels of ROS and NO produced by susceptible vs. resistant rat strain, and investigated their relevancy for arthritis development and severity. In addition, we have stimulated macrophages in vitro with Mycobacterium bovis BCG, and two heat shock proteins (HSP): endogenous HSP47 and mycobacterial HSP71 (rnHSP71). Our results suggest a possible contribution of increased ROS production to arthritis resistance of AO rats. The ROS production in AO rats is potentiated by endogenous HSP47, but not with mycobacterial cell and mHSP71, suggesting HSP47 participates in AA control. We have found no fundamental relationship between the magnitude of NO production and AA and OIA susceptibility and severity, suggesting that NO has no effector role in AA and OIA. Our results advocate a regulatory type action of NO molecule aught be more significant in arthritis development. (c) 2006 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Munich
T2  - Immunobiology
T1  - Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats
EP  - 105
IS  - 2
SP  - 95
VL  - 212
DO  - 10.1016/j.imbio.2006.11.012
ER  - 

@article{
author = "Miletić, Tatjana and Kovačević-Jovanović, Vesna and Vujić, Vesna and Stanojević, Stanislava and Mitić, Katarina and Lazarević-Macanović, Miriana and Dimitrijević, Mirjana",
year = "2007",
abstract = "There is extensive evidence for the critical role of reactive oxygen species (ROS) and nitric oxide (NO) produced by phagocytes in development of inflammatory processes and pathogenesis of numerous diseases, including rheumatoid arthritis (RA). Apart from their function as mediators of inflammation and tissue damage, recent research supports their role as signaling and regulatory molecules. In the present study we have investigated the production of ROS and NO over the course of adjuvant arthritis (AA) and oil-induced arthritis (OIA), by resident peritoneal macrophages of two rat strains: Dark Agouti (DA), susceptible, and Albino Oxford (AO), resistant to induction of AA and OIA. We have compared levels of ROS and NO produced by susceptible vs. resistant rat strain, and investigated their relevancy for arthritis development and severity. In addition, we have stimulated macrophages in vitro with Mycobacterium bovis BCG, and two heat shock proteins (HSP): endogenous HSP47 and mycobacterial HSP71 (rnHSP71). Our results suggest a possible contribution of increased ROS production to arthritis resistance of AO rats. The ROS production in AO rats is potentiated by endogenous HSP47, but not with mycobacterial cell and mHSP71, suggesting HSP47 participates in AA control. We have found no fundamental relationship between the magnitude of NO production and AA and OIA susceptibility and severity, suggesting that NO has no effector role in AA and OIA. Our results advocate a regulatory type action of NO molecule aught be more significant in arthritis development. (c) 2006 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Munich",
journal = "Immunobiology",
title = "Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats",
pages = "105-95",
number = "2",
volume = "212",
doi = "10.1016/j.imbio.2006.11.012"
}

Miletić, T., Kovačević-Jovanović, V., Vujić, V., Stanojević, S., Mitić, K., Lazarević-Macanović, M.,& Dimitrijević, M.. (2007). Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats. in Immunobiology
Elsevier Gmbh, Munich., 212(2), 95-105.
https://doi.org/10.1016/j.imbio.2006.11.012

Miletić T, Kovačević-Jovanović V, Vujić V, Stanojević S, Mitić K, Lazarević-Macanović M, Dimitrijević M. Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats. in Immunobiology. 2007;212(2):95-105.
doi:10.1016/j.imbio.2006.11.012 .

Miletić, Tatjana, Kovačević-Jovanović, Vesna, Vujić, Vesna, Stanojević, Stanislava, Mitić, Katarina, Lazarević-Macanović, Miriana, Dimitrijević, Mirjana, "Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats" in Immunobiology, 212, no. 2 (2007):95-105,
https://doi.org/10.1016/j.imbio.2006.11.012 . .

TY  - JOUR
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Dimitrijević, Mirjana
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/137
AB  - It is well documented that endogenous opioid peptides modulate the activity of immune and inflammatory cells. Opioid peptides exert biological effects through at least three opioid receptor types, known as µ, δ and κ. In the present study we have investigated which types of opioid receptors are involved in the opioid peptide-induced modulation of superoxide an/on release from macrophages. Rat peritoneal macrophages were stimulated with zymosan, and treated in vitro with opioid peptides and/or antagonists specific for different opioid receptor types. The results showed that the µ opioid receptor agonist, beta-endorphin, and the k opioid receptor agonist dynorphin A, decreased superoxide anion production. Conversely methionine-enkephalin (Met-Enk) and leucine-enkephalin, that have a preference for δ opioid receptors, did not affect superoxide anion release. Furthermore, we have tested the effect of Met-Enk on superoxide anion release from macrophages under blockade of all receptors with specific antagonists except for one receptor subtype. Accordingly, it was found that the Met-Enk induced increase and decrease of superoxide anion production were mediated through δ1,2 and µκ opioid receptors, respectively. It can be concluded that opposing activity of µ, and κ receptors vs. δ receptors together with the affinity for a ceflain receptor-type determines the effect of a particular endogenous opioid peptide on superoxide anion release from rat peritoneal macrophages.
AB  - Poznato je da endogeni opioidni peptidi modulišu aktivnost imunskih i inflamatornih ćelija. Opioidni peptidi ostvaruju biološke efekte preko tri tipa receptora, µ, δ i κ. U ovom radu ispitivali smo koji tipovi opioidnih receptora učestvuju u opioidnoj modulaciji oslobadanjasuperoksid anjona iz peritonealnih makrofaga. Peritonealne makrofage pacova su stimulisane zimozanom, i tretirane in vitro sa opioidnim peptidima i/ili specifičnim antagonistima različitih tipova opioidnih receptora. Rezultati su pokazali da beta-endorphin, agonist µ, opiodnih receptora, i dynorphin A, agonist κ opiodnih receptora, smanjuju produkciju superoksid anjona. S druge strane metionin-enkefalin (Met-Enk) i leucin-enkefalin, koji se prevashodno vezuju za δ opioidne receptore, nisu uticali na oslobadanje superoksid anjona. Pored toga, ispitivali smo uticaj Met-Enk na oslobađanje superoksid anjona iz makrofaga u uslovima blokade svih receptora sa specifičnim antagonistima izuzev jednog tipa/podtipa opioidnog receptora. Shodno tome pokazali smo da Met-Enk povećava oslobadanje superoksid anjona preko δ1,2 a smanjuje oslobadanje superoksid anjona preko µk opioidnih receptora. Može se zaključiti da je uticaj endogenih opioidnih peptida na oslobadanje superoksid anjona iz peritonealnih makrofaga pacova određen suprotnom aktivnošću (µ i k receptora u odnosu na δ receptore, kao i različitim afinitetom peptida za pojedine tipove opioidnih receptora.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors
T1  - Uticaj opioidnih peptida na produkciju superoksid anjona u peritonealnim makrofagama pacova stimulisanim zimozanom - uloga µ, δ i κ opioidnih receptora
EP  - 78
IS  - 2-3
SP  - 69
VL  - 52
DO  - 10.2298/AVB0203069V
ER  - 

@article{
author = "Vujić, Vesna and Stanojević, Stanislava and Dimitrijević, Mirjana",
year = "2002",
abstract = "It is well documented that endogenous opioid peptides modulate the activity of immune and inflammatory cells. Opioid peptides exert biological effects through at least three opioid receptor types, known as µ, δ and κ. In the present study we have investigated which types of opioid receptors are involved in the opioid peptide-induced modulation of superoxide an/on release from macrophages. Rat peritoneal macrophages were stimulated with zymosan, and treated in vitro with opioid peptides and/or antagonists specific for different opioid receptor types. The results showed that the µ opioid receptor agonist, beta-endorphin, and the k opioid receptor agonist dynorphin A, decreased superoxide anion production. Conversely methionine-enkephalin (Met-Enk) and leucine-enkephalin, that have a preference for δ opioid receptors, did not affect superoxide anion release. Furthermore, we have tested the effect of Met-Enk on superoxide anion release from macrophages under blockade of all receptors with specific antagonists except for one receptor subtype. Accordingly, it was found that the Met-Enk induced increase and decrease of superoxide anion production were mediated through δ1,2 and µκ opioid receptors, respectively. It can be concluded that opposing activity of µ, and κ receptors vs. δ receptors together with the affinity for a ceflain receptor-type determines the effect of a particular endogenous opioid peptide on superoxide anion release from rat peritoneal macrophages., Poznato je da endogeni opioidni peptidi modulišu aktivnost imunskih i inflamatornih ćelija. Opioidni peptidi ostvaruju biološke efekte preko tri tipa receptora, µ, δ i κ. U ovom radu ispitivali smo koji tipovi opioidnih receptora učestvuju u opioidnoj modulaciji oslobadanjasuperoksid anjona iz peritonealnih makrofaga. Peritonealne makrofage pacova su stimulisane zimozanom, i tretirane in vitro sa opioidnim peptidima i/ili specifičnim antagonistima različitih tipova opioidnih receptora. Rezultati su pokazali da beta-endorphin, agonist µ, opiodnih receptora, i dynorphin A, agonist κ opiodnih receptora, smanjuju produkciju superoksid anjona. S druge strane metionin-enkefalin (Met-Enk) i leucin-enkefalin, koji se prevashodno vezuju za δ opioidne receptore, nisu uticali na oslobadanje superoksid anjona. Pored toga, ispitivali smo uticaj Met-Enk na oslobađanje superoksid anjona iz makrofaga u uslovima blokade svih receptora sa specifičnim antagonistima izuzev jednog tipa/podtipa opioidnog receptora. Shodno tome pokazali smo da Met-Enk povećava oslobadanje superoksid anjona preko δ1,2 a smanjuje oslobadanje superoksid anjona preko µk opioidnih receptora. Može se zaključiti da je uticaj endogenih opioidnih peptida na oslobadanje superoksid anjona iz peritonealnih makrofaga pacova određen suprotnom aktivnošću (µ i k receptora u odnosu na δ receptore, kao i različitim afinitetom peptida za pojedine tipove opioidnih receptora.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors, Uticaj opioidnih peptida na produkciju superoksid anjona u peritonealnim makrofagama pacova stimulisanim zimozanom - uloga µ, δ i κ opioidnih receptora",
pages = "78-69",
number = "2-3",
volume = "52",
doi = "10.2298/AVB0203069V"
}

Vujić, V., Stanojević, S.,& Dimitrijević, M.. (2002). The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 52(2-3), 69-78.
https://doi.org/10.2298/AVB0203069V

Vujić V, Stanojević S, Dimitrijević M. The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors. in Acta veterinaria - Beograd. 2002;52(2-3):69-78.
doi:10.2298/AVB0203069V .

Vujić, Vesna, Stanojević, Stanislava, Dimitrijević, Mirjana, "The effect of opioid peptides on superoxide, anion production in rat peritoneal macrophages stimulated with zymosan: involvement of µ, δ and κ opioid receptors" in Acta veterinaria - Beograd, 52, no. 2-3 (2002):69-78,
https://doi.org/10.2298/AVB0203069V . .