Živančević-Simonović, Snežana

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  • Živančević-Simonović, Snežana (10)
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The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction

Mihaljević, Olgica; Živančević-Simonović, Snežana; Lučić-Tomić, Aleksandra; Živković, Irena; Minić, Rajna; Mijatović-Teodorović, Ljiljana; Jovanović, Zorica; Anđelković, Marija; Stanojević-Pirković, Marijana

(Društvo medicinskih biohemičara Srbije, Beograd i Versita, 2020)

TY  - JOUR
AU  - Mihaljević, Olgica
AU  - Živančević-Simonović, Snežana
AU  - Lučić-Tomić, Aleksandra
AU  - Živković, Irena
AU  - Minić, Rajna
AU  - Mijatović-Teodorović, Ljiljana
AU  - Jovanović, Zorica
AU  - Anđelković, Marija
AU  - Stanojević-Pirković, Marijana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/558
AB  - Background: The aim of this study was to compare serum sclerostin concentrations in patients with thyroid dysfunction with euthyroid control subjects and to assess the relationship between sclerostin and markers of bone metabolism (osteocalcin and beta-cross-laps). Methods: The study included 30 patients with thyroid dysfunction (hypothyroidism, hyperthyroidism and subclinical hyperthyroidism) and ten euthyroid controls. Free thyroxine (FT4) was measured by radioimmunoassay, while thyroid stimulating hormone (TSH) concentration was determined immunoradiometrically. We used an ELISA kit to determine the sclerostin level. The electrochemiluminescence method was applied for measuring the bone markers. Results: Sclerostin levels were significantly lower in hypothyroid patients (p=0.009) and significantly elevated in hyperthyroid patients (p=0.008) compared to control values. Hyperthyroid patients also had higher sclerostin than patients with subclinical hyperthyroidism (p=0.013). Sclerostin concentrations were negatively correlated with TSH levels (r=-0.746, p lt 0.001), but positively with FT4 (r=0.696, p  lt  0.001). Moreover, sclerostin was positively associated with osteocalcin (r=0.605, p=0.005) and beta-cross-laps levels (r=0.573, p=0.008) in all thyroid patients. Conclusions: Serum sclerostin is significantly affected in subjects with thyroid dysfunction. Both sclerostin and thyroid status affect bone homeostasis, which is reflected through the significant correlations with osteocalcin and beta-cross-laps.
AB  - Uvod: Cilj ove studije bio je da uporedimo serumske koncentracije sklerostina kod pacijenata sa disfunkcijom štitaste žlezde u odnosu na eutiroidne kontrolne ispitanike, i da procenimo odnos između sklerostina i markera koštanog metabolizma (osteokalcina i beta-cross-lapsa) u istoj populaciji. Metode: Studijom je obuhvaćeno 30 pacijenata sa disfunkcijom štitaste žlezde (hipotireozom, hipertireozom i supkliničkom hipertireozom) i 10 eutiroidnih kontrola. Slobodni tiroksin (FT4) meren je radioimunološkom metodom, dok je koncentracija tireostimulišućeg hormona (TSH) određivana imunoradiometrijski. Za merenje nivoa sklerostina koristili smo ELISA-test. Metoda elektrohemiluminiscencije primenjena je za merenje koncentracije koštanih markera. Rezultati: Nivo sklerostina bio je značajno niži kod bolesnika sa hipotireozom (p = 0,009) odnosno značajno viši kod bolesnika sa hipertireozom (p = 0,008) u poređenju sa vrednostima kod eutiroidnih kontrolnih ispitanika. Pacijenti sa hipertireozom su takođe imali statistički značajno viši nivo sklerostina u odnosu na bolesnike sa supkliničkom hipertireozom (p = 0,013). Pokazana je negativna korelacija koncentracije sklerostina i TSH (r = -0,746, p  lt  0,001), odnosno pozitivna korelacija sa FT4 (r = 0,696, p  lt  0,001) kod pacijenata sa tireoidnom disfunkcijom. Štaviše, sklerostin pozitivno korelira sa koncentracijom osteokalcina (r = 0,605, p = 0,005) i beta-cross-lapsa (r = 0,573, p = 0,008) kod ovih pacijenata. Zaključak: Serumski nivoi sklerostina izmenjeni su kod osoba sa disfunkcijom štitaste žlezde. Sklerostin zajedno sa tireoidnim statusom utiče na koštani metabolizam, što se ogleda u njegovoj značajnoj korelaciji sa osteokalcinom i beta-cross-lapsom.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction
T1  - Veza cirkulišućeg nivoa sklerostina sa markerima metabolizma kostiju kod pacijenata sa poremećajem rada štitaste žlezde
EP  - 443
IS  - 4
SP  - 436
VL  - 39
DO  - 10.5937/jomb0-24943
UR  - conv_72
ER  - 
@article{
author = "Mihaljević, Olgica and Živančević-Simonović, Snežana and Lučić-Tomić, Aleksandra and Živković, Irena and Minić, Rajna and Mijatović-Teodorović, Ljiljana and Jovanović, Zorica and Anđelković, Marija and Stanojević-Pirković, Marijana",
year = "2020",
abstract = "Background: The aim of this study was to compare serum sclerostin concentrations in patients with thyroid dysfunction with euthyroid control subjects and to assess the relationship between sclerostin and markers of bone metabolism (osteocalcin and beta-cross-laps). Methods: The study included 30 patients with thyroid dysfunction (hypothyroidism, hyperthyroidism and subclinical hyperthyroidism) and ten euthyroid controls. Free thyroxine (FT4) was measured by radioimmunoassay, while thyroid stimulating hormone (TSH) concentration was determined immunoradiometrically. We used an ELISA kit to determine the sclerostin level. The electrochemiluminescence method was applied for measuring the bone markers. Results: Sclerostin levels were significantly lower in hypothyroid patients (p=0.009) and significantly elevated in hyperthyroid patients (p=0.008) compared to control values. Hyperthyroid patients also had higher sclerostin than patients with subclinical hyperthyroidism (p=0.013). Sclerostin concentrations were negatively correlated with TSH levels (r=-0.746, p lt 0.001), but positively with FT4 (r=0.696, p  lt  0.001). Moreover, sclerostin was positively associated with osteocalcin (r=0.605, p=0.005) and beta-cross-laps levels (r=0.573, p=0.008) in all thyroid patients. Conclusions: Serum sclerostin is significantly affected in subjects with thyroid dysfunction. Both sclerostin and thyroid status affect bone homeostasis, which is reflected through the significant correlations with osteocalcin and beta-cross-laps., Uvod: Cilj ove studije bio je da uporedimo serumske koncentracije sklerostina kod pacijenata sa disfunkcijom štitaste žlezde u odnosu na eutiroidne kontrolne ispitanike, i da procenimo odnos između sklerostina i markera koštanog metabolizma (osteokalcina i beta-cross-lapsa) u istoj populaciji. Metode: Studijom je obuhvaćeno 30 pacijenata sa disfunkcijom štitaste žlezde (hipotireozom, hipertireozom i supkliničkom hipertireozom) i 10 eutiroidnih kontrola. Slobodni tiroksin (FT4) meren je radioimunološkom metodom, dok je koncentracija tireostimulišućeg hormona (TSH) određivana imunoradiometrijski. Za merenje nivoa sklerostina koristili smo ELISA-test. Metoda elektrohemiluminiscencije primenjena je za merenje koncentracije koštanih markera. Rezultati: Nivo sklerostina bio je značajno niži kod bolesnika sa hipotireozom (p = 0,009) odnosno značajno viši kod bolesnika sa hipertireozom (p = 0,008) u poređenju sa vrednostima kod eutiroidnih kontrolnih ispitanika. Pacijenti sa hipertireozom su takođe imali statistički značajno viši nivo sklerostina u odnosu na bolesnike sa supkliničkom hipertireozom (p = 0,013). Pokazana je negativna korelacija koncentracije sklerostina i TSH (r = -0,746, p  lt  0,001), odnosno pozitivna korelacija sa FT4 (r = 0,696, p  lt  0,001) kod pacijenata sa tireoidnom disfunkcijom. Štaviše, sklerostin pozitivno korelira sa koncentracijom osteokalcina (r = 0,605, p = 0,005) i beta-cross-lapsa (r = 0,573, p = 0,008) kod ovih pacijenata. Zaključak: Serumski nivoi sklerostina izmenjeni su kod osoba sa disfunkcijom štitaste žlezde. Sklerostin zajedno sa tireoidnim statusom utiče na koštani metabolizam, što se ogleda u njegovoj značajnoj korelaciji sa osteokalcinom i beta-cross-lapsom.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction, Veza cirkulišućeg nivoa sklerostina sa markerima metabolizma kostiju kod pacijenata sa poremećajem rada štitaste žlezde",
pages = "443-436",
number = "4",
volume = "39",
doi = "10.5937/jomb0-24943",
url = "conv_72"
}
Mihaljević, O., Živančević-Simonović, S., Lučić-Tomić, A., Živković, I., Minić, R., Mijatović-Teodorović, L., Jovanović, Z., Anđelković, M.,& Stanojević-Pirković, M.. (2020). The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 39(4), 436-443.
https://doi.org/10.5937/jomb0-24943
conv_72
Mihaljević O, Živančević-Simonović S, Lučić-Tomić A, Živković I, Minić R, Mijatović-Teodorović L, Jovanović Z, Anđelković M, Stanojević-Pirković M. The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction. in Journal of Medical Biochemistry. 2020;39(4):436-443.
doi:10.5937/jomb0-24943
conv_72 .
Mihaljević, Olgica, Živančević-Simonović, Snežana, Lučić-Tomić, Aleksandra, Živković, Irena, Minić, Rajna, Mijatović-Teodorović, Ljiljana, Jovanović, Zorica, Anđelković, Marija, Stanojević-Pirković, Marijana, "The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction" in Journal of Medical Biochemistry, 39, no. 4 (2020):436-443,
https://doi.org/10.5937/jomb0-24943 .,
conv_72 .
1
2

Correlation of sera concentra tions of thyroperoxidase a utoantibodies measured by two radioimmunoassays

Vrndić, Olgica; Živančević-Simonović, Snežana; Dimitrijević, Ljiljana; Đukić, Aleksandar; Arsenijević, Nebojša

(Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad, 2010)

TY  - JOUR
AU  - Vrndić, Olgica
AU  - Živančević-Simonović, Snežana
AU  - Dimitrijević, Ljiljana
AU  - Đukić, Aleksandar
AU  - Arsenijević, Nebojša
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/307
AB  - Introduction. Thyroid peroxidase-specific autoantibodies (TPO Abs) are mostly measured in patients with autoimmune thyroid diseases. The aim of this study was to compare TPO Ab concentrations measured by two radioimmunoassays. Material and methods. Our investigation included 38 patients. Sera concentrations of TPO Abs were measured by using Cis biointernational (France) and Immunotech (Czech Republic) assays. Results. Concentrations obtained by two assays were extensively different. The values measured by Cis biointernational assay were higher than ones obtained by Immunotech assay. The statistical arrangement of results showed the direct correlation between the two assays, with the coefficient of agreement R=0.6239 (p lt 0.001). The analysis of relative values (ratio of measured and upper limit values given by the manufacturer) demonstrated the statistically significant difference (p=0.003) between values measured by Cis biointernational (18.94±37.22) and by Immunotech assay (4.22±8.22) concerning the distinction between normal and raised concentrations of TPO Abs. The agreement of results (enhanced or normal TPO Ab concentrations in both tests) was shown in 30 sera samples (78.95%), but in residual 8 sera (21.05%) normal TPO Ab concentrations were obtained by Immunotech, and enchanced by Cis biointernational assay. There is no difference in capability of distinction between normal and pathological results between the two tests (χ2=3.484, p gt 0.05). The highest concentration of TPO Ab measured by Cis biointernational assay was not the highest one in Immunotech assay, which might be a reflection of different specificity of antibodies used in two diagnostic tests. Conclusion. TPO Ab concentrations obtained by Cis biointernational and Immunotech assays are very different. In several sera samples, normal concentrations of TPO autoantibodies were obtained by Immunotech assay and enhanced by Cis biointernational assay. The highest value obtained by one is not the highest value measured by another assay we used.
AB  - Autontitela specifična za tiroidnu peroksidazu autoantitela specifičnih za tiroidnu peroksidazu prevashodno se određuju radi dijagnoze autoimunih bolesti štitaste žlezde. Cilj rada bio je da se uporede koncentracije autoantitela specifičnih za tiroidnu peroksidazu dobijene korišćenjem dva testa: Cis bionternational (Francuska) i Immunotech (Češka Republika). Ispitivanjem je obuhvaćeno 38 ispitanika. Iako su se koncentracije autoantitela specifičnih za tiroidnu peroksidazu u ispitivanim serumima znatno razlikovale i u apsolutnim i u relativnim vrednostima, statističkom obradom rezultata pokazana je direktna korelacija rezultata merenja ova dva testa, sa koeficijentom R=0,6239 (p lt 0,001). Na osnovu analize relativnih vrednosti. pokazana je statistički značajna razlika (p=0,003) između srednjih vrednosti rezultata izmerenih testovima Cis (18,94±37,22) i Immunotech (4,22±8,22). Slaganje rezultata pokazano je u 78,95% seruma, dok je u 21,05% seruma testom Immunotech dobijena normalna, a testom Cis biointernational granična ili povećana koncentracija antitela. Statistički podaci su pokazali da se testovi ne razlikuju po razdvajanju patoloških od normalnih vrednosti (χ2=3,484, p gt 0,05). Iako koncentracije autoantitela specifičnih za tiroidnu peroksidazu izmerene pomoću testova Cis biointernational i testom Immunotech pokazuju značajan stepen korelacije, njihove i apsolutne i relativne vrednosti znatno se razlikuju.
PB  - Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad
T2  - Medicinski pregled
T1  - Correlation of sera concentra tions of thyroperoxidase a utoantibodies measured by two radioimmunoassays
T1  - Korelacija koncentracija autoantitela specifičnih za tiroidnu peroksidazu određenih korišćenjem dva radioimunološka testa
EP  - 108
IS  - 1-2
SP  - 104
VL  - 63
DO  - 10.2298/MPNS1002104V
UR  - conv_5
ER  - 
@article{
author = "Vrndić, Olgica and Živančević-Simonović, Snežana and Dimitrijević, Ljiljana and Đukić, Aleksandar and Arsenijević, Nebojša",
year = "2010",
abstract = "Introduction. Thyroid peroxidase-specific autoantibodies (TPO Abs) are mostly measured in patients with autoimmune thyroid diseases. The aim of this study was to compare TPO Ab concentrations measured by two radioimmunoassays. Material and methods. Our investigation included 38 patients. Sera concentrations of TPO Abs were measured by using Cis biointernational (France) and Immunotech (Czech Republic) assays. Results. Concentrations obtained by two assays were extensively different. The values measured by Cis biointernational assay were higher than ones obtained by Immunotech assay. The statistical arrangement of results showed the direct correlation between the two assays, with the coefficient of agreement R=0.6239 (p lt 0.001). The analysis of relative values (ratio of measured and upper limit values given by the manufacturer) demonstrated the statistically significant difference (p=0.003) between values measured by Cis biointernational (18.94±37.22) and by Immunotech assay (4.22±8.22) concerning the distinction between normal and raised concentrations of TPO Abs. The agreement of results (enhanced or normal TPO Ab concentrations in both tests) was shown in 30 sera samples (78.95%), but in residual 8 sera (21.05%) normal TPO Ab concentrations were obtained by Immunotech, and enchanced by Cis biointernational assay. There is no difference in capability of distinction between normal and pathological results between the two tests (χ2=3.484, p gt 0.05). The highest concentration of TPO Ab measured by Cis biointernational assay was not the highest one in Immunotech assay, which might be a reflection of different specificity of antibodies used in two diagnostic tests. Conclusion. TPO Ab concentrations obtained by Cis biointernational and Immunotech assays are very different. In several sera samples, normal concentrations of TPO autoantibodies were obtained by Immunotech assay and enhanced by Cis biointernational assay. The highest value obtained by one is not the highest value measured by another assay we used., Autontitela specifična za tiroidnu peroksidazu autoantitela specifičnih za tiroidnu peroksidazu prevashodno se određuju radi dijagnoze autoimunih bolesti štitaste žlezde. Cilj rada bio je da se uporede koncentracije autoantitela specifičnih za tiroidnu peroksidazu dobijene korišćenjem dva testa: Cis bionternational (Francuska) i Immunotech (Češka Republika). Ispitivanjem je obuhvaćeno 38 ispitanika. Iako su se koncentracije autoantitela specifičnih za tiroidnu peroksidazu u ispitivanim serumima znatno razlikovale i u apsolutnim i u relativnim vrednostima, statističkom obradom rezultata pokazana je direktna korelacija rezultata merenja ova dva testa, sa koeficijentom R=0,6239 (p lt 0,001). Na osnovu analize relativnih vrednosti. pokazana je statistički značajna razlika (p=0,003) između srednjih vrednosti rezultata izmerenih testovima Cis (18,94±37,22) i Immunotech (4,22±8,22). Slaganje rezultata pokazano je u 78,95% seruma, dok je u 21,05% seruma testom Immunotech dobijena normalna, a testom Cis biointernational granična ili povećana koncentracija antitela. Statistički podaci su pokazali da se testovi ne razlikuju po razdvajanju patoloških od normalnih vrednosti (χ2=3,484, p gt 0,05). Iako koncentracije autoantitela specifičnih za tiroidnu peroksidazu izmerene pomoću testova Cis biointernational i testom Immunotech pokazuju značajan stepen korelacije, njihove i apsolutne i relativne vrednosti znatno se razlikuju.",
publisher = "Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad",
journal = "Medicinski pregled",
title = "Correlation of sera concentra tions of thyroperoxidase a utoantibodies measured by two radioimmunoassays, Korelacija koncentracija autoantitela specifičnih za tiroidnu peroksidazu određenih korišćenjem dva radioimunološka testa",
pages = "108-104",
number = "1-2",
volume = "63",
doi = "10.2298/MPNS1002104V",
url = "conv_5"
}
Vrndić, O., Živančević-Simonović, S., Dimitrijević, L., Đukić, A.,& Arsenijević, N.. (2010). Correlation of sera concentra tions of thyroperoxidase a utoantibodies measured by two radioimmunoassays. in Medicinski pregled
Društvo lekara Vojvodine Srpskog lekarskog društva, Novi Sad., 63(1-2), 104-108.
https://doi.org/10.2298/MPNS1002104V
conv_5
Vrndić O, Živančević-Simonović S, Dimitrijević L, Đukić A, Arsenijević N. Correlation of sera concentra tions of thyroperoxidase a utoantibodies measured by two radioimmunoassays. in Medicinski pregled. 2010;63(1-2):104-108.
doi:10.2298/MPNS1002104V
conv_5 .
Vrndić, Olgica, Živančević-Simonović, Snežana, Dimitrijević, Ljiljana, Đukić, Aleksandar, Arsenijević, Nebojša, "Correlation of sera concentra tions of thyroperoxidase a utoantibodies measured by two radioimmunoassays" in Medicinski pregled, 63, no. 1-2 (2010):104-108,
https://doi.org/10.2298/MPNS1002104V .,
conv_5 .

Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases

Kostić, Irena; Živančević-Simonović, Snežana; Bukilica, Mirjana; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2006)

TY  - JOUR
AU  - Kostić, Irena
AU  - Živančević-Simonović, Snežana
AU  - Bukilica, Mirjana
AU  - Dimitrijević, Ljiljana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/221
AB  - Autoimmune thyroid disease (ATD) has been described in patients with connective tissue diseases (CTD). The aim of this study was to estimate and compare the prevalence of ATD in a group of 91 CTD patients, and in their subgroups: 53 systemic lupus erythematosus (SLE), 24 rheumatoid arthritis (RA), 7 primary Sjogren’s syndrome (SSy) and 7 progressive systemic sclerosis (SSc) patients. A control group of 34 healthy blood volunteers was used for comparison. Serum levels of free thyroxine (FT4), thyroid stimulating hormone (TSH), as well as thyroid autoantibodies (Abs) specific of thyroperoxidase (TPO) and thyroglobulin (TG) were examined. CTD patients, in general, as well as SLE and RA subgroups, had significantly higher number of thyroid dysfunction than the control group (p lt 0.05). The most prominent thyroid dysfunction was subclinical hypothyroidism, with a higher prevalence in all subgroups of patients when compared to the control. Anti-TPO Abs were detected in a significant number of CTD patients, especially in SLE subgroup when compared to the control group. It was also found that a higher number of CTD patients, SLE and RA subgroups, had positive anti-Tg Abs, when compared to the control subjects. In conclusion, the prevalence of ATD in CTD patients was more frequent than in the control group. The patients with anti-TPO Abs and anti-Tg Abs at the time when they were analyzed, had hyperthyroidism, hypothyroidism or were clinically and biochemically euthyroid. The prevalence of hypothyroidism was greater than the prevalence of hyperthyroidism in all subgroups of patients.
AB  - Autoimunske bolesti štitaste žlezde (AITD) opisane su kod bolesnika sa sistemskim bolestima vezivnog tkiva (SBVT). Cilj ovog rada bio je da se ispita prevalencija AITD u grupi od 91 bolesnika sa SBVT, koja je uključila 53 bolesnika sa sistemskim eritemskim lupusom (SLE), 24 obolelih sa reumatoidnim artritisom (RA) i po 7 obolelih od primarnog Sjogrenovog sindroma (SSy) i progresivne sistemske skleroze (SSc). Kontrolnu grupu ispitanika činila su 34 dobrovoljna davaoca krvi. Kod svih učesnika u studiji merene su serumske koncentracije slobodnog tiroksina (FT4), tireostimulišućeg hormona (TSH), kao i autoantitela specifičnih za tireoperoksidazu (anti-TPO At) i tireoglobulin (anti-Tg At). U grupi bolesnika sa SBVT, kao i u podgrupama obolelih od SLE i RA, nađena je statistički značajno veća učestalost poremećaja funkcije štitaste žlezde u odnosu na kontrolnu grupu (p lt 0.05), a od svih poremećaja funkcije štitaste žlezde najčečće je detektovana subklinička hipotireoza. Anti-TPO At nađena su kod značajno većeg broja bolesnika sa SBVT i SLE, u odnosu na kontrolnu grupu. Isto tako, i anti-Tg At su detektovana kod većeg broja ispitanika u grupi SBVT, i podgrupama SLE i RA, nego kod zdravih osoba. U zaključku, prevalencija AITD kod obolelih od SBVT veća je nego u kontrolnoj grupi. Bolesnici sa anti- TPO At i anti-Tg At u vreme kada su analizirani, imali su subkliničku ili klinički manifestnu hipotireozu ili hipertireozu, ili su još bili u stadijumu bolesti u kome se ne može detektovati poremećaj funkcije štitaste žlezde. Prevalencija hipotireoze bila je veća od prevalencije hipertireoze u svim podgrupama bolesnika sa SBVT.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases
T1  - Funkcija štitaste žlezde i prisustvo antitireoidnih autoantitela kod bolesnika sa sistemskim bolestima vezivnog tkiva
EP  - 64
IS  - 2
SP  - 61
VL  - 7
UR  - conv_70
ER  - 
@article{
author = "Kostić, Irena and Živančević-Simonović, Snežana and Bukilica, Mirjana and Dimitrijević, Ljiljana",
year = "2006",
abstract = "Autoimmune thyroid disease (ATD) has been described in patients with connective tissue diseases (CTD). The aim of this study was to estimate and compare the prevalence of ATD in a group of 91 CTD patients, and in their subgroups: 53 systemic lupus erythematosus (SLE), 24 rheumatoid arthritis (RA), 7 primary Sjogren’s syndrome (SSy) and 7 progressive systemic sclerosis (SSc) patients. A control group of 34 healthy blood volunteers was used for comparison. Serum levels of free thyroxine (FT4), thyroid stimulating hormone (TSH), as well as thyroid autoantibodies (Abs) specific of thyroperoxidase (TPO) and thyroglobulin (TG) were examined. CTD patients, in general, as well as SLE and RA subgroups, had significantly higher number of thyroid dysfunction than the control group (p lt 0.05). The most prominent thyroid dysfunction was subclinical hypothyroidism, with a higher prevalence in all subgroups of patients when compared to the control. Anti-TPO Abs were detected in a significant number of CTD patients, especially in SLE subgroup when compared to the control group. It was also found that a higher number of CTD patients, SLE and RA subgroups, had positive anti-Tg Abs, when compared to the control subjects. In conclusion, the prevalence of ATD in CTD patients was more frequent than in the control group. The patients with anti-TPO Abs and anti-Tg Abs at the time when they were analyzed, had hyperthyroidism, hypothyroidism or were clinically and biochemically euthyroid. The prevalence of hypothyroidism was greater than the prevalence of hyperthyroidism in all subgroups of patients., Autoimunske bolesti štitaste žlezde (AITD) opisane su kod bolesnika sa sistemskim bolestima vezivnog tkiva (SBVT). Cilj ovog rada bio je da se ispita prevalencija AITD u grupi od 91 bolesnika sa SBVT, koja je uključila 53 bolesnika sa sistemskim eritemskim lupusom (SLE), 24 obolelih sa reumatoidnim artritisom (RA) i po 7 obolelih od primarnog Sjogrenovog sindroma (SSy) i progresivne sistemske skleroze (SSc). Kontrolnu grupu ispitanika činila su 34 dobrovoljna davaoca krvi. Kod svih učesnika u studiji merene su serumske koncentracije slobodnog tiroksina (FT4), tireostimulišućeg hormona (TSH), kao i autoantitela specifičnih za tireoperoksidazu (anti-TPO At) i tireoglobulin (anti-Tg At). U grupi bolesnika sa SBVT, kao i u podgrupama obolelih od SLE i RA, nađena je statistički značajno veća učestalost poremećaja funkcije štitaste žlezde u odnosu na kontrolnu grupu (p lt 0.05), a od svih poremećaja funkcije štitaste žlezde najčečće je detektovana subklinička hipotireoza. Anti-TPO At nađena su kod značajno većeg broja bolesnika sa SBVT i SLE, u odnosu na kontrolnu grupu. Isto tako, i anti-Tg At su detektovana kod većeg broja ispitanika u grupi SBVT, i podgrupama SLE i RA, nego kod zdravih osoba. U zaključku, prevalencija AITD kod obolelih od SBVT veća je nego u kontrolnoj grupi. Bolesnici sa anti- TPO At i anti-Tg At u vreme kada su analizirani, imali su subkliničku ili klinički manifestnu hipotireozu ili hipertireozu, ili su još bili u stadijumu bolesti u kome se ne može detektovati poremećaj funkcije štitaste žlezde. Prevalencija hipotireoze bila je veća od prevalencije hipertireoze u svim podgrupama bolesnika sa SBVT.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases, Funkcija štitaste žlezde i prisustvo antitireoidnih autoantitela kod bolesnika sa sistemskim bolestima vezivnog tkiva",
pages = "64-61",
number = "2",
volume = "7",
url = "conv_70"
}
Kostić, I., Živančević-Simonović, S., Bukilica, M.,& Dimitrijević, L.. (2006). Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 7(2), 61-64.
conv_70
Kostić I, Živančević-Simonović S, Bukilica M, Dimitrijević L. Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases. in Medicus. 2006;7(2):61-64.
conv_70 .
Kostić, Irena, Živančević-Simonović, Snežana, Bukilica, Mirjana, Dimitrijević, Ljiljana, "Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases" in Medicus, 7, no. 2 (2006):61-64,
conv_70 .
3

Properties of tissue specific macrophages before and after in vitro activation

Živančević-Simonović, Snežana; Inić-Kanada, Aleksandra; Stojanović, Marijana; Dimitrijević, Ljiljana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2004)

TY  - JOUR
AU  - Živančević-Simonović, Snežana
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Dimitrijević, Ljiljana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/189
AB  - Macrophages derived from different tissues: bone marrow, spleen, peritoneal cavity and alveolus, were examined from the aspects of their morphology and functional characteristics expression of Fc receptors (FcR), phagocytic activity towards yeast particles and nonspecific esterase (NSE) content¹ before and after in vitro activation. Twenty four-hour-adherent cells were isolated with the aim of analyzing the characteristics of resident tissue macrophages. Following cultivation in vitro 8-day-adherent cells were used to investigate the influence of macrophage activation on their morphology and function. Morphological analysis of cell smears, performed in respect to cell size, showed significant enlargement, especially in the population of alveolar cells cultured for 8 days and activated with colony-stimulating factors (CSFs) and lymphokines. It was also demonstrated that 24-hour- and 8-dayadherent macrophages derived from different tissues exhibited similar properties. All these cells were more than 90% FcR-positive (FcR+) NSE-positive (NSE+) and had phagocytic properties. However, within the population of alveolar macrophages there were some NSE+ cells lacking FcR and phagocytic activity, even after in vitro activation. These results confirmed that the properties of alveolar macrophages differing from those of macrophages from other tissues were dependent on their microenvironment.
AB  - Morfološke i funkcionalne karakteristike makrofaga izolovanih iz različitih organa: kostne srži, slezine, peritonealne šupljine i alveola ispitivane su pre i posle aktivacije u in vitro uslovima. Da bi se ispitale karakteristike makrofaga koji se nalaze u ispitivanim tkivima analizirane su adherentne ćelije dobijene nakon inkubacije od 24 časa. Uticaj aktivacije makrofaga na njihovu morfologiju i funkciju (ekspresiju Fc receptora, sposobnost fagocitoze čestica kvasca i sadržaj enzima nespecifične esteraze) ispitivan je nakon kultivisanja adherentnih ćelija u toku 8 dana. Morfološkom analizom utvrđeno je značajno povećanje veličine alveolarnih makrofaga kultivisanih tokom 8 dana u prisustvu faktora koji stimulišu rast kolonija i limfokina. Pokazano je da adherentne ćelije iz različitih tkiva izolovane nakon 24 sata i 8 dana imaju slične funkcionalne karakteristike. Više od 90% tih ćelija je eksprimiralo Fc receptore, imalo sposobnost fagocitoze i sadržavalo nespecifičnu esterazu. Međutim, u populaciji alveolarnih makrofaga, pre kao i nakon in vitro aktivacije, utvđeno je prisustvo ćelija koje su sadržavale nespecifičnu esterazu u citoplazmi, ali nisu eksprimirale Fc receptore, niti su imale sposobnost fagocitoze. Ovi rezultati potvrđuju da su karakteristike alveolarnih makrofaga u odnosu na makrofage iz drugih tkiva zavisne od njihovog mikrookruženja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Properties of tissue specific macrophages before and after in vitro activation
T1  - Karakteristike tkivno specifičnih makrofaga pre i posle aktivacije in vitro
EP  - 31
IS  - 1
SP  - 21
VL  - 54
DO  - 10.2298/AVB0401021Z
UR  - conv_48
ER  - 
@article{
author = "Živančević-Simonović, Snežana and Inić-Kanada, Aleksandra and Stojanović, Marijana and Dimitrijević, Ljiljana",
year = "2004",
abstract = "Macrophages derived from different tissues: bone marrow, spleen, peritoneal cavity and alveolus, were examined from the aspects of their morphology and functional characteristics expression of Fc receptors (FcR), phagocytic activity towards yeast particles and nonspecific esterase (NSE) content¹ before and after in vitro activation. Twenty four-hour-adherent cells were isolated with the aim of analyzing the characteristics of resident tissue macrophages. Following cultivation in vitro 8-day-adherent cells were used to investigate the influence of macrophage activation on their morphology and function. Morphological analysis of cell smears, performed in respect to cell size, showed significant enlargement, especially in the population of alveolar cells cultured for 8 days and activated with colony-stimulating factors (CSFs) and lymphokines. It was also demonstrated that 24-hour- and 8-dayadherent macrophages derived from different tissues exhibited similar properties. All these cells were more than 90% FcR-positive (FcR+) NSE-positive (NSE+) and had phagocytic properties. However, within the population of alveolar macrophages there were some NSE+ cells lacking FcR and phagocytic activity, even after in vitro activation. These results confirmed that the properties of alveolar macrophages differing from those of macrophages from other tissues were dependent on their microenvironment., Morfološke i funkcionalne karakteristike makrofaga izolovanih iz različitih organa: kostne srži, slezine, peritonealne šupljine i alveola ispitivane su pre i posle aktivacije u in vitro uslovima. Da bi se ispitale karakteristike makrofaga koji se nalaze u ispitivanim tkivima analizirane su adherentne ćelije dobijene nakon inkubacije od 24 časa. Uticaj aktivacije makrofaga na njihovu morfologiju i funkciju (ekspresiju Fc receptora, sposobnost fagocitoze čestica kvasca i sadržaj enzima nespecifične esteraze) ispitivan je nakon kultivisanja adherentnih ćelija u toku 8 dana. Morfološkom analizom utvrđeno je značajno povećanje veličine alveolarnih makrofaga kultivisanih tokom 8 dana u prisustvu faktora koji stimulišu rast kolonija i limfokina. Pokazano je da adherentne ćelije iz različitih tkiva izolovane nakon 24 sata i 8 dana imaju slične funkcionalne karakteristike. Više od 90% tih ćelija je eksprimiralo Fc receptore, imalo sposobnost fagocitoze i sadržavalo nespecifičnu esterazu. Međutim, u populaciji alveolarnih makrofaga, pre kao i nakon in vitro aktivacije, utvđeno je prisustvo ćelija koje su sadržavale nespecifičnu esterazu u citoplazmi, ali nisu eksprimirale Fc receptore, niti su imale sposobnost fagocitoze. Ovi rezultati potvrđuju da su karakteristike alveolarnih makrofaga u odnosu na makrofage iz drugih tkiva zavisne od njihovog mikrookruženja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Properties of tissue specific macrophages before and after in vitro activation, Karakteristike tkivno specifičnih makrofaga pre i posle aktivacije in vitro",
pages = "31-21",
number = "1",
volume = "54",
doi = "10.2298/AVB0401021Z",
url = "conv_48"
}
Živančević-Simonović, S., Inić-Kanada, A., Stojanović, M.,& Dimitrijević, L.. (2004). Properties of tissue specific macrophages before and after in vitro activation. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(1), 21-31.
https://doi.org/10.2298/AVB0401021Z
conv_48
Živančević-Simonović S, Inić-Kanada A, Stojanović M, Dimitrijević L. Properties of tissue specific macrophages before and after in vitro activation. in Acta veterinaria - Beograd. 2004;54(1):21-31.
doi:10.2298/AVB0401021Z
conv_48 .
Živančević-Simonović, Snežana, Inić-Kanada, Aleksandra, Stojanović, Marijana, Dimitrijević, Ljiljana, "Properties of tissue specific macrophages before and after in vitro activation" in Acta veterinaria - Beograd, 54, no. 1 (2004):21-31,
https://doi.org/10.2298/AVB0401021Z .,
conv_48 .
1
2
1

Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages

Živančević-Simonović, Snežana; Inić-Kanada, Aleksandra; Stojanović, Marijana; Dimitrijević, Ljiljana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2004)

TY  - JOUR
AU  - Živančević-Simonović, Snežana
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Dimitrijević, Ljiljana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/187
AB  - In this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number of macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation.
AB  - U ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovelo je do smanjenja T limfocitne proliferacije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages
T1  - Proliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofaga
EP  - 335
IS  - 5-6
SP  - 327
VL  - 54
DO  - 10.2298/AVB0406327Z
UR  - conv_50
ER  - 
@article{
author = "Živančević-Simonović, Snežana and Inić-Kanada, Aleksandra and Stojanović, Marijana and Dimitrijević, Ljiljana",
year = "2004",
abstract = "In this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number of macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation., U ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovelo je do smanjenja T limfocitne proliferacije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages, Proliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofaga",
pages = "335-327",
number = "5-6",
volume = "54",
doi = "10.2298/AVB0406327Z",
url = "conv_50"
}
Živančević-Simonović, S., Inić-Kanada, A., Stojanović, M.,& Dimitrijević, L.. (2004). Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(5-6), 327-335.
https://doi.org/10.2298/AVB0406327Z
conv_50
Živančević-Simonović S, Inić-Kanada A, Stojanović M, Dimitrijević L. Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages. in Acta veterinaria - Beograd. 2004;54(5-6):327-335.
doi:10.2298/AVB0406327Z
conv_50 .
Živančević-Simonović, Snežana, Inić-Kanada, Aleksandra, Stojanović, Marijana, Dimitrijević, Ljiljana, "Proliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophages" in Acta veterinaria - Beograd, 54, no. 5-6 (2004):327-335,
https://doi.org/10.2298/AVB0406327Z .,
conv_50 .

Role of the oxidative stress in pathophysiology of immune system

Živančević-Simonović, Snežana; Đukić, Aleksandar; Inić-Kanada, Aleksandra; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2004)

TY  - JOUR
AU  - Živančević-Simonović, Snežana
AU  - Đukić, Aleksandar
AU  - Inić-Kanada, Aleksandra
AU  - Dimitrijević, Ljiljana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/182
AB  - Free radicals are generated as the byproducts of many physiological cellular reactions or accidentally. Biologically, the most relevant free radicals are highly reactive oxygen and nitric molecules. These molecules can establish chain reactions causing damage of cell membranes, proteins and nucleic acids. Numerous antioxidative mechanisms exist in order to control their effects and maintain redox homeostasis. The situation in which the cellular redox homoeostasis is altered, i.e. the balance between pro-oxidants and antioxidants, is known as the "oxidative stress". An oxidative stress maybe induced by the activation of endogenous generating systems or by conditions generated by environmental factors. The response to increased levels of ROS is known as "oxidative stress response". In cases of persistently high ROS levels the loss of homeostasis might be developed which could result m pathological conditions. In this study, we review the main mechanisms that generate free radicals and lead to oxidative stress conditions included in aging and pathogenesis of many different disorders. Because of the involvement of the immune system m many of these diseases, a particular attention was focused on oxidative stress influence on both natural and acquired immunity, with the special emphasis on free radical influence on T cell activation and survival.
AB  - Slobodni radikali se stvaraju kao uzgredni produkti mnogih fizioloških procesa u ćelijama ili zadesno. Najveći biološki značaj imaju reaktivni oblici kiseonika i azota. Ti molekuli mogu izazvati lančane reakcije koje prouzrokuju oštećenje ćelijskih membrana, proteina i nukleinskih kiselina. Da bi se kontrolisali njihovi efekti i održala redox homeostaza, postoje brojni zaštitni antioksidativni mehanizmi. "Oksidativni stres" predstavlja stanje u kome je narušena redoks homeostaza, odnosno balans između pro-oksidativnih i antioksidativnih supstanci. Oksidativni stres može biti prouzrokovan aktivacijom endogenih mehanizama stvaranja slobodnih radikala ili dejstvom egzogenih faktora. Ćelijske reakcije na povećanje koncentracije reaktivnih oblika kiseonika predstavljaju "odgovor na Oksidativni stres". Ako se povećana koncentracija reaktivnih obuka kiseonika održava uprkos aktivaciji antioksidativnih mehanizama, narušava se redox homeostaza i može doći do pojave bolesti. U ovom radu su prikazani glavni mehanizmi stvaranja slobodnih radikala koji vode nastanku oksidativnog stresa uključenog u proces starenja i nastanak mnogobrojnih i raznovrsnih bolesti. S obzirom na ulogu imunskog sistema u njihovoj patogenezi, posebno je prikazan uticaj oksidativnog stresa na urođene i stečene imunske mehanizme, kao i uticaj slobodnih radikala na aktivaciju i preživljavanje T linfocita.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - Role of the oxidative stress in pathophysiology of immune system
T1  - Uloga oksidativnog stresa u patofiziologiji imunskog sistema
EP  - 16
IS  - 2
SP  - 11
VL  - 5
UR  - conv_69
ER  - 
@article{
author = "Živančević-Simonović, Snežana and Đukić, Aleksandar and Inić-Kanada, Aleksandra and Dimitrijević, Ljiljana",
year = "2004",
abstract = "Free radicals are generated as the byproducts of many physiological cellular reactions or accidentally. Biologically, the most relevant free radicals are highly reactive oxygen and nitric molecules. These molecules can establish chain reactions causing damage of cell membranes, proteins and nucleic acids. Numerous antioxidative mechanisms exist in order to control their effects and maintain redox homeostasis. The situation in which the cellular redox homoeostasis is altered, i.e. the balance between pro-oxidants and antioxidants, is known as the "oxidative stress". An oxidative stress maybe induced by the activation of endogenous generating systems or by conditions generated by environmental factors. The response to increased levels of ROS is known as "oxidative stress response". In cases of persistently high ROS levels the loss of homeostasis might be developed which could result m pathological conditions. In this study, we review the main mechanisms that generate free radicals and lead to oxidative stress conditions included in aging and pathogenesis of many different disorders. Because of the involvement of the immune system m many of these diseases, a particular attention was focused on oxidative stress influence on both natural and acquired immunity, with the special emphasis on free radical influence on T cell activation and survival., Slobodni radikali se stvaraju kao uzgredni produkti mnogih fizioloških procesa u ćelijama ili zadesno. Najveći biološki značaj imaju reaktivni oblici kiseonika i azota. Ti molekuli mogu izazvati lančane reakcije koje prouzrokuju oštećenje ćelijskih membrana, proteina i nukleinskih kiselina. Da bi se kontrolisali njihovi efekti i održala redox homeostaza, postoje brojni zaštitni antioksidativni mehanizmi. "Oksidativni stres" predstavlja stanje u kome je narušena redoks homeostaza, odnosno balans između pro-oksidativnih i antioksidativnih supstanci. Oksidativni stres može biti prouzrokovan aktivacijom endogenih mehanizama stvaranja slobodnih radikala ili dejstvom egzogenih faktora. Ćelijske reakcije na povećanje koncentracije reaktivnih oblika kiseonika predstavljaju "odgovor na Oksidativni stres". Ako se povećana koncentracija reaktivnih obuka kiseonika održava uprkos aktivaciji antioksidativnih mehanizama, narušava se redox homeostaza i može doći do pojave bolesti. U ovom radu su prikazani glavni mehanizmi stvaranja slobodnih radikala koji vode nastanku oksidativnog stresa uključenog u proces starenja i nastanak mnogobrojnih i raznovrsnih bolesti. S obzirom na ulogu imunskog sistema u njihovoj patogenezi, posebno je prikazan uticaj oksidativnog stresa na urođene i stečene imunske mehanizme, kao i uticaj slobodnih radikala na aktivaciju i preživljavanje T linfocita.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "Role of the oxidative stress in pathophysiology of immune system, Uloga oksidativnog stresa u patofiziologiji imunskog sistema",
pages = "16-11",
number = "2",
volume = "5",
url = "conv_69"
}
Živančević-Simonović, S., Đukić, A., Inić-Kanada, A.,& Dimitrijević, L.. (2004). Role of the oxidative stress in pathophysiology of immune system. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 5(2), 11-16.
conv_69
Živančević-Simonović S, Đukić A, Inić-Kanada A, Dimitrijević L. Role of the oxidative stress in pathophysiology of immune system. in Medicus. 2004;5(2):11-16.
conv_69 .
Živančević-Simonović, Snežana, Đukić, Aleksandar, Inić-Kanada, Aleksandra, Dimitrijević, Ljiljana, "Role of the oxidative stress in pathophysiology of immune system" in Medicus, 5, no. 2 (2004):11-16,
conv_69 .

Autoimmune thyroid diseases: In vivo diagnostics

Živančević-Simonović, Snežana; Đukić, Aleksandar; Matović, Milovan D.; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2004)

TY  - JOUR
AU  - Živančević-Simonović, Snežana
AU  - Đukić, Aleksandar
AU  - Matović, Milovan D.
AU  - Dimitrijević, Ljiljana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/185
AB  - Autoimmune thyroid disease, Graves disease and Hashimoto thyroiditis, cause changing in morphology and function of the thyroid tissue. Graves disease is characterized by an increased synthesis and release of thyroid hormones (hyperthyrosis). Hashimoto fhyroiditis is characterized by the destruction and regeneration of thyroid follicles, which are clinically expressed by symptoms of hypothyrosis. Less frequently, Hashimoto fhyroiditis is expressed by transient thyrotoxicosis (if the destruction of the thyroid gland tissue is extremely emphasized). In order to examine thyroid morphology and function, there are numerous in iga diagnostic methods. Ultrasono graphy enables the examination of the fine structure and vasculanzation of the thyroid gland, and nuclear medicine methods reflect the function of the thyroid tissue. Radioactive iodine uptake is useful in assessment of the ability of thyrocytes to uptake iodide, and scmtigraphy gives the morpho-functional picture of the thyroid gland. Scintigraphy of orbital tissue gives the insight of orbital accumulation of the activated leucocytes. Although modem imaging methods (positron emission tomography, computed tomography and magnetic resonance imaging) are very useful in assessment of nodular changes in the thyroid gland and retrostemal goiter, these techniques are not widely applied in diagnostic procedures of autoimmune thyroid diseases. In this study we reviewed in vivo diagnostic methods used in the examination of Graves disease and Hashimoto thyroiditis, we considered their significance in the investigation of pathological process in the thyroid gland and we noticed the factors which could influence the results of morphological and functional investigations of the thyroid gland.
AB  - Autoimunske bolesti štitaste žlezde, Gravesova bolest i Hashimoto tireoiditis, prouzrokuju promenu strukture i funkcije tireoidnog tkiva. Gravesovu bolest karakteriše povećana sinteza i oslobađanje tireoidnih hormona (hipemreoza), a Hashimoto tireoiditis destrukcija i regeneracija tireoidnih folikula, koje se klinički ispoljavaju simptomima hipotireoze, a ako je destrukcija tkiva štitaste žlezde veoma izražena, privremeno mogu da se jave i simptomi tireotoksikoze. Da bi se ispitala struktura i funkcija tireoidnog tkiva primenjuju se brojne in vivo dijagnostičke metode. Ultrasonografija daje uvid u finu strukturu i vaskularizaciju štitaste žlezde, a metode nuklearne medicine odslikavaju funkciju tireoidnog tkiva. Testom fiksacije radioaktivnog joda procenjuje se sposobnost tireocita da preuzmu jodid, a scintigrafijom se dobija morfofunkcijska slika štitaste žlezde. Scintigrafijom orbite kod obolelih od Gravesove bolesti ispituje se stepen infiltracije orbitalnog tkiva aktivisanim leukocitima. Iako su savremene imaging metode (pozitronska emisiona tomografija, kompjuterizovana tomografija i magnetna rezonanca) veoma korisne u ispitivanju nodoznih promena u štitastoj žlezdi i substemame strume, te metode nisu značajnije zastupljene u dijagnostici autoimunskih tireoidnih bolesti. U ovom radu su prikazane in vivo dijagnostičke metode koje se koriste u dijagnostici Gravesove bolesti i Hashimoto tireoiditisa, njihov značaj u ispitivanju patološkog procesa u štitastoj žlezdi i faktori koji svojim dejstvom mogu da utiču na rezultate ispitivanja.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - Autoimmune thyroid diseases: In vivo diagnostics
T1  - Autoimunske bolesti štitaste žlezde - in vivo dijagnostika
EP  - 33
IS  - 1
SP  - 27
VL  - 5
UR  - conv_68
ER  - 
@article{
author = "Živančević-Simonović, Snežana and Đukić, Aleksandar and Matović, Milovan D. and Dimitrijević, Ljiljana",
year = "2004",
abstract = "Autoimmune thyroid disease, Graves disease and Hashimoto thyroiditis, cause changing in morphology and function of the thyroid tissue. Graves disease is characterized by an increased synthesis and release of thyroid hormones (hyperthyrosis). Hashimoto fhyroiditis is characterized by the destruction and regeneration of thyroid follicles, which are clinically expressed by symptoms of hypothyrosis. Less frequently, Hashimoto fhyroiditis is expressed by transient thyrotoxicosis (if the destruction of the thyroid gland tissue is extremely emphasized). In order to examine thyroid morphology and function, there are numerous in iga diagnostic methods. Ultrasono graphy enables the examination of the fine structure and vasculanzation of the thyroid gland, and nuclear medicine methods reflect the function of the thyroid tissue. Radioactive iodine uptake is useful in assessment of the ability of thyrocytes to uptake iodide, and scmtigraphy gives the morpho-functional picture of the thyroid gland. Scintigraphy of orbital tissue gives the insight of orbital accumulation of the activated leucocytes. Although modem imaging methods (positron emission tomography, computed tomography and magnetic resonance imaging) are very useful in assessment of nodular changes in the thyroid gland and retrostemal goiter, these techniques are not widely applied in diagnostic procedures of autoimmune thyroid diseases. In this study we reviewed in vivo diagnostic methods used in the examination of Graves disease and Hashimoto thyroiditis, we considered their significance in the investigation of pathological process in the thyroid gland and we noticed the factors which could influence the results of morphological and functional investigations of the thyroid gland., Autoimunske bolesti štitaste žlezde, Gravesova bolest i Hashimoto tireoiditis, prouzrokuju promenu strukture i funkcije tireoidnog tkiva. Gravesovu bolest karakteriše povećana sinteza i oslobađanje tireoidnih hormona (hipemreoza), a Hashimoto tireoiditis destrukcija i regeneracija tireoidnih folikula, koje se klinički ispoljavaju simptomima hipotireoze, a ako je destrukcija tkiva štitaste žlezde veoma izražena, privremeno mogu da se jave i simptomi tireotoksikoze. Da bi se ispitala struktura i funkcija tireoidnog tkiva primenjuju se brojne in vivo dijagnostičke metode. Ultrasonografija daje uvid u finu strukturu i vaskularizaciju štitaste žlezde, a metode nuklearne medicine odslikavaju funkciju tireoidnog tkiva. Testom fiksacije radioaktivnog joda procenjuje se sposobnost tireocita da preuzmu jodid, a scintigrafijom se dobija morfofunkcijska slika štitaste žlezde. Scintigrafijom orbite kod obolelih od Gravesove bolesti ispituje se stepen infiltracije orbitalnog tkiva aktivisanim leukocitima. Iako su savremene imaging metode (pozitronska emisiona tomografija, kompjuterizovana tomografija i magnetna rezonanca) veoma korisne u ispitivanju nodoznih promena u štitastoj žlezdi i substemame strume, te metode nisu značajnije zastupljene u dijagnostici autoimunskih tireoidnih bolesti. U ovom radu su prikazane in vivo dijagnostičke metode koje se koriste u dijagnostici Gravesove bolesti i Hashimoto tireoiditisa, njihov značaj u ispitivanju patološkog procesa u štitastoj žlezdi i faktori koji svojim dejstvom mogu da utiču na rezultate ispitivanja.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "Autoimmune thyroid diseases: In vivo diagnostics, Autoimunske bolesti štitaste žlezde - in vivo dijagnostika",
pages = "33-27",
number = "1",
volume = "5",
url = "conv_68"
}
Živančević-Simonović, S., Đukić, A., Matović, M. D.,& Dimitrijević, L.. (2004). Autoimmune thyroid diseases: In vivo diagnostics. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 5(1), 27-33.
conv_68
Živančević-Simonović S, Đukić A, Matović MD, Dimitrijević L. Autoimmune thyroid diseases: In vivo diagnostics. in Medicus. 2004;5(1):27-33.
conv_68 .
Živančević-Simonović, Snežana, Đukić, Aleksandar, Matović, Milovan D., Dimitrijević, Ljiljana, "Autoimmune thyroid diseases: In vivo diagnostics" in Medicus, 5, no. 1 (2004):27-33,
conv_68 .

Auto immune thyroid disease: The pathogenesis of Graves disease and Hashimoto thyroiditis

Živančević-Simonović, Snežana; Đukić, Aleksandar; Arsenijević, Nebojša; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2003)

TY  - JOUR
AU  - Živančević-Simonović, Snežana
AU  - Đukić, Aleksandar
AU  - Arsenijević, Nebojša
AU  - Dimitrijević, Ljiljana
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/164
AB  - It is generally accepted that autoimmune thyroid disorders, Graves disease and Hashimoto thyroiditis, differ in pathogenesis and clinical implications. In Graves disease the basic pathogenetic mechanism is B lymphocyte activation which produce auto antibodies specific for TSH receptor (TSHR) which binding to thyrocyte membrane causes their long-termed stimulation which gives as a result the occurrence of hyperthyrosis. On the other hand in Hashimoto thyroiditis lymphocyte accumulation occurs and they cause gradual thyrocyte damage and hypothyrosis development. However, it was found that the reisa certain genetic predisposition for both autoimmune thyroid diseases and that they can appear among several members of the same family. Besides in the serum of the patients with Graves disease and Hashimoto thyroiditis the presence of autoantibodi esspecific for dominant thyroid autoantigenes: TSHR, thyroperoxidase (TPO) and thyroglobulin (Tg) can be found as indicators of the auto immune process in thyroid gland. For these reasons both autoimmune diseases of thyroid gland sometimes are marked with the common name: autoimmune thyroid disease. As cell and molecule mechanisms included in initiation of the autoimmune process in thyroid gland that define the type and natural course of disease have not completely been explained, in this review the data from the literature considering pathogenesis of autoimmune diseases of thyroid gland have been shown. After introductory considerations, dominant autoantigenes and autoantibodies (as indicators of autoimmune process in thyroid gland) are shown in details, as well as mechanisms included in effector phase of autoimmune process which cau se the thyroid cell damage. The role of disturbance in regulation of the apoptosis process is especially analyzed as they could effect the development of autoimmune diseases of thyroid gland.
AB  - Prihvaćeno je shvatanje da se autoimunske bolesti štitaste žlezde Graves-ova bolest i Hashimoto tireoiditis razlikuju po patogenezi i kliničkim posledicama. U Graves-ovoj bolesti je osnovni patogenetski mehanizam aktivacija Blimfocita koji produkuju auto antitela specifična za TSH receptor (TSHR), čije vezivanje za membranu tireocita uzrokuje njihovu dugotrajnu stimulaciju, sa posledičnim nastankom hipertireoze. S druge strane, u Hashimoto tireoiditi su nastaje akumulacija limfocita koji prouzrokuju postepeno oštećenje tireocita i nastanak hipotireoze. Međutim utvrđeno je da za obe autoimunske tireoidne bolesti postoji određena genetska predispozicija i da se one mogu javiti kod više članova u istoj porodici. Osim toga, u serumu obolelih od Graves-ove bolesti i Hashimoto tireoiditi sa se može pokazati prisustvo autoantitela specifičnih za dominantne tireoidne autoantigene (receptor za TSH, tireoperoksidazu i tireoglobulin) koji predstavljaju pokazatelje autoimunskog procesa u štitastoj žlezdi. Iz tih razloga se obe autoimunske bolesti štitaste žlezde nekad označavaju zajedničkim nazivom: autoimunska bolest štitaste žlezde. Budući da ćelijski i molekulski mehanizmi koji su uključeni u inicijaciju autoimunskog procesa u štita stoj žlezdi, i opredeljuju vrstu i prirodni tok bolesti, nisu potpuno rasvetljeni, u ovom radu su prikazani podaci iz literature koji se odnose na patogenezu autoimunskih bolesti štitaste žlezde. Nakon uvodnih razmatranja, detaljno su prikazani dominantni autoantigeni i autoantitela (kao pokazatelji autoimunskog procesa u štitastoj žlezdi), kao i mehanizmi uključeni u efektorsku fazu autoimunskog procesa koji uzrokuju oštećenje tireocita. Posebno je analizirana uloga poremećaja u regulaciji procesa apoptoze u nastanku autoimunskih bolesti štitaste žlezde.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - Auto immune thyroid disease: The pathogenesis of Graves disease and Hashimoto thyroiditis
T1  - Auto imunska bolest štitaste žlezde - patogeneza Graves-ove bolesti i Hashimoto tireoiditisa
EP  - 26
IS  - 1
SP  - 21
VL  - 4
UR  - conv_66
ER  - 
@article{
author = "Živančević-Simonović, Snežana and Đukić, Aleksandar and Arsenijević, Nebojša and Dimitrijević, Ljiljana",
year = "2003",
abstract = "It is generally accepted that autoimmune thyroid disorders, Graves disease and Hashimoto thyroiditis, differ in pathogenesis and clinical implications. In Graves disease the basic pathogenetic mechanism is B lymphocyte activation which produce auto antibodies specific for TSH receptor (TSHR) which binding to thyrocyte membrane causes their long-termed stimulation which gives as a result the occurrence of hyperthyrosis. On the other hand in Hashimoto thyroiditis lymphocyte accumulation occurs and they cause gradual thyrocyte damage and hypothyrosis development. However, it was found that the reisa certain genetic predisposition for both autoimmune thyroid diseases and that they can appear among several members of the same family. Besides in the serum of the patients with Graves disease and Hashimoto thyroiditis the presence of autoantibodi esspecific for dominant thyroid autoantigenes: TSHR, thyroperoxidase (TPO) and thyroglobulin (Tg) can be found as indicators of the auto immune process in thyroid gland. For these reasons both autoimmune diseases of thyroid gland sometimes are marked with the common name: autoimmune thyroid disease. As cell and molecule mechanisms included in initiation of the autoimmune process in thyroid gland that define the type and natural course of disease have not completely been explained, in this review the data from the literature considering pathogenesis of autoimmune diseases of thyroid gland have been shown. After introductory considerations, dominant autoantigenes and autoantibodies (as indicators of autoimmune process in thyroid gland) are shown in details, as well as mechanisms included in effector phase of autoimmune process which cau se the thyroid cell damage. The role of disturbance in regulation of the apoptosis process is especially analyzed as they could effect the development of autoimmune diseases of thyroid gland., Prihvaćeno je shvatanje da se autoimunske bolesti štitaste žlezde Graves-ova bolest i Hashimoto tireoiditis razlikuju po patogenezi i kliničkim posledicama. U Graves-ovoj bolesti je osnovni patogenetski mehanizam aktivacija Blimfocita koji produkuju auto antitela specifična za TSH receptor (TSHR), čije vezivanje za membranu tireocita uzrokuje njihovu dugotrajnu stimulaciju, sa posledičnim nastankom hipertireoze. S druge strane, u Hashimoto tireoiditi su nastaje akumulacija limfocita koji prouzrokuju postepeno oštećenje tireocita i nastanak hipotireoze. Međutim utvrđeno je da za obe autoimunske tireoidne bolesti postoji određena genetska predispozicija i da se one mogu javiti kod više članova u istoj porodici. Osim toga, u serumu obolelih od Graves-ove bolesti i Hashimoto tireoiditi sa se može pokazati prisustvo autoantitela specifičnih za dominantne tireoidne autoantigene (receptor za TSH, tireoperoksidazu i tireoglobulin) koji predstavljaju pokazatelje autoimunskog procesa u štitastoj žlezdi. Iz tih razloga se obe autoimunske bolesti štitaste žlezde nekad označavaju zajedničkim nazivom: autoimunska bolest štitaste žlezde. Budući da ćelijski i molekulski mehanizmi koji su uključeni u inicijaciju autoimunskog procesa u štita stoj žlezdi, i opredeljuju vrstu i prirodni tok bolesti, nisu potpuno rasvetljeni, u ovom radu su prikazani podaci iz literature koji se odnose na patogenezu autoimunskih bolesti štitaste žlezde. Nakon uvodnih razmatranja, detaljno su prikazani dominantni autoantigeni i autoantitela (kao pokazatelji autoimunskog procesa u štitastoj žlezdi), kao i mehanizmi uključeni u efektorsku fazu autoimunskog procesa koji uzrokuju oštećenje tireocita. Posebno je analizirana uloga poremećaja u regulaciji procesa apoptoze u nastanku autoimunskih bolesti štitaste žlezde.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "Auto immune thyroid disease: The pathogenesis of Graves disease and Hashimoto thyroiditis, Auto imunska bolest štitaste žlezde - patogeneza Graves-ove bolesti i Hashimoto tireoiditisa",
pages = "26-21",
number = "1",
volume = "4",
url = "conv_66"
}
Živančević-Simonović, S., Đukić, A., Arsenijević, N.,& Dimitrijević, L.. (2003). Auto immune thyroid disease: The pathogenesis of Graves disease and Hashimoto thyroiditis. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 4(1), 21-26.
conv_66
Živančević-Simonović S, Đukić A, Arsenijević N, Dimitrijević L. Auto immune thyroid disease: The pathogenesis of Graves disease and Hashimoto thyroiditis. in Medicus. 2003;4(1):21-26.
conv_66 .
Živančević-Simonović, Snežana, Đukić, Aleksandar, Arsenijević, Nebojša, Dimitrijević, Ljiljana, "Auto immune thyroid disease: The pathogenesis of Graves disease and Hashimoto thyroiditis" in Medicus, 4, no. 1 (2003):21-26,
conv_66 .

Autoimmune thyroid diseases: In vitro diagnostics

Živančević-Simonović, Snežana; Đukić, Aleksandar; Matović, Milovan D.; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2003)

TY  - JOUR
AU  - Živančević-Simonović, Snežana
AU  - Đukić, Aleksandar
AU  - Matović, Milovan D.
AU  - Dimitrijević, Ljiljana
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/158
AB  - In diagnostics of autoimmune thyroid diseases a number of in vitro methods is used to evaluate thyroid function or examine the presence of an tithyroid antibodies which may be included in pathogenesis of the disease or only represent "silent witnesses" of autoimmune processes. During the evaluation of thyroid function serum concentrations of thyroid-stimulating hormone (TSH) and thyroid hormones are determined and due to these concentrations the diagnosis of manifested or subclinical disturbances of thyroid gland is set. Detection of antibodies specific in dominant thyroid auto antigenes represents confirmation of autoimmune pathogenesis of the disease. The concentration of TSH and thyroid hormones in blood may be influenced by some diseases and drugs, so abnormal values can be detected in absence of diseases of thyroid gland. In the serum of a tested person heterophilic antibodies, rheumatoid factors or other antibodies may occur which reduce the number of specific interactions in the assay, causing increased or decreased concentrations of hormones or autoantibodies. A great number of methods used in in vitro diagnostics differs very much by sensitivity which should be considered during the interpretation of laboratory results. This study presents the most important methods used in diagnostics of autoimmune thyroid diseases.
AB  - U dijagnostici autoimunskih bolesti štitaste žlezde koriste se brojne in vitro metode kojima se procenjuje tireoidna funkcija ili ispituje prisustvo antitireoidnih autoantitela koja mogu biti uključena u patogenezu bolesti ili samo predstavljati "neme svedoke" autoimunskog procesa. Pri proceni funkcije štitaste žlezde određuju se serumske koncentracije tireostimulišućeg (TSH) i tireoidnih hormona i na osnovu njihove koncentracije postavlja dijagnoza ispoljenih ili subkliničkih poremećajafunkcije štitaste žlezde (hipertireoze ili hipotireoze). Potvrdu autoimunske patogeneze bolesti predstavlja detekcija antitela specifičnih za dominantne tireoidne autoantigene (receptor za TSH, tireoidnu peroksida zuitireoglobulin). Na koncentraciju TSH i tireoidnih hormona u krvi mogu uticati neka fiziološka stanja, bolesti i lekovi, tako da se abnormalne vrednosti mogu detektovati i u odsustvu bolesti štitaste žlezde. U serumu ispitanika mogu biti prisutna heterofilna anti tela, reumatoidni faktori ili druga auto anti tela koja smanjuju broj specifičnih interakcija u testu prouzrokujući povećanu ili smanjenu koncentraciju hormona ili autoantitela. Veliki broj metoda koje se koriste u in vitro dijagnostici veoma se razlikuju prema osetljivosti, što treba imati u vidu prilikom interpretacije laboratorijskih rezultata. U ovom radu su prikazane najvažnije metode koje se koriste u dijagnostici autoimunskih bolesti štitaste žlezde: Graves-ove bolesti i Hashimoto tireoiditisa.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - Autoimmune thyroid diseases: In vitro diagnostics
T1  - Autoimunske bolesti štitaste žlezde - in vitro dijagnostika
EP  - 30
IS  - 2
SP  - 23
VL  - 4
UR  - conv_67
ER  - 
@article{
author = "Živančević-Simonović, Snežana and Đukić, Aleksandar and Matović, Milovan D. and Dimitrijević, Ljiljana",
year = "2003",
abstract = "In diagnostics of autoimmune thyroid diseases a number of in vitro methods is used to evaluate thyroid function or examine the presence of an tithyroid antibodies which may be included in pathogenesis of the disease or only represent "silent witnesses" of autoimmune processes. During the evaluation of thyroid function serum concentrations of thyroid-stimulating hormone (TSH) and thyroid hormones are determined and due to these concentrations the diagnosis of manifested or subclinical disturbances of thyroid gland is set. Detection of antibodies specific in dominant thyroid auto antigenes represents confirmation of autoimmune pathogenesis of the disease. The concentration of TSH and thyroid hormones in blood may be influenced by some diseases and drugs, so abnormal values can be detected in absence of diseases of thyroid gland. In the serum of a tested person heterophilic antibodies, rheumatoid factors or other antibodies may occur which reduce the number of specific interactions in the assay, causing increased or decreased concentrations of hormones or autoantibodies. A great number of methods used in in vitro diagnostics differs very much by sensitivity which should be considered during the interpretation of laboratory results. This study presents the most important methods used in diagnostics of autoimmune thyroid diseases., U dijagnostici autoimunskih bolesti štitaste žlezde koriste se brojne in vitro metode kojima se procenjuje tireoidna funkcija ili ispituje prisustvo antitireoidnih autoantitela koja mogu biti uključena u patogenezu bolesti ili samo predstavljati "neme svedoke" autoimunskog procesa. Pri proceni funkcije štitaste žlezde određuju se serumske koncentracije tireostimulišućeg (TSH) i tireoidnih hormona i na osnovu njihove koncentracije postavlja dijagnoza ispoljenih ili subkliničkih poremećajafunkcije štitaste žlezde (hipertireoze ili hipotireoze). Potvrdu autoimunske patogeneze bolesti predstavlja detekcija antitela specifičnih za dominantne tireoidne autoantigene (receptor za TSH, tireoidnu peroksida zuitireoglobulin). Na koncentraciju TSH i tireoidnih hormona u krvi mogu uticati neka fiziološka stanja, bolesti i lekovi, tako da se abnormalne vrednosti mogu detektovati i u odsustvu bolesti štitaste žlezde. U serumu ispitanika mogu biti prisutna heterofilna anti tela, reumatoidni faktori ili druga auto anti tela koja smanjuju broj specifičnih interakcija u testu prouzrokujući povećanu ili smanjenu koncentraciju hormona ili autoantitela. Veliki broj metoda koje se koriste u in vitro dijagnostici veoma se razlikuju prema osetljivosti, što treba imati u vidu prilikom interpretacije laboratorijskih rezultata. U ovom radu su prikazane najvažnije metode koje se koriste u dijagnostici autoimunskih bolesti štitaste žlezde: Graves-ove bolesti i Hashimoto tireoiditisa.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "Autoimmune thyroid diseases: In vitro diagnostics, Autoimunske bolesti štitaste žlezde - in vitro dijagnostika",
pages = "30-23",
number = "2",
volume = "4",
url = "conv_67"
}
Živančević-Simonović, S., Đukić, A., Matović, M. D.,& Dimitrijević, L.. (2003). Autoimmune thyroid diseases: In vitro diagnostics. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 4(2), 23-30.
conv_67
Živančević-Simonović S, Đukić A, Matović MD, Dimitrijević L. Autoimmune thyroid diseases: In vitro diagnostics. in Medicus. 2003;4(2):23-30.
conv_67 .
Živančević-Simonović, Snežana, Đukić, Aleksandar, Matović, Milovan D., Dimitrijević, Ljiljana, "Autoimmune thyroid diseases: In vitro diagnostics" in Medicus, 4, no. 2 (2003):23-30,
conv_67 .

The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities

Inić-Kanada, Aleksandra; Stojanović, Marijana; Šeatović, Saša S.; Živković, Irena; Jankov, Ratko; Živančević-Simonović, Snežana; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2002)

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Šeatović, Saša S.
AU  - Živković, Irena
AU  - Jankov, Ratko
AU  - Živančević-Simonović, Snežana
AU  - Dimitrijević, Ljiljana
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/154
AB  - Monoclonal antibodies (MoAb) are usually produced as the secretion products of cloned B lymphoblastoid cell lines. Advantages of MoAbs are that they could be selected to have desired specificity and produced in relatively large quantities of consistent quality and char acteristics. Interest in the production of re agents specific for human kappa (K) chain can be explained by the fact that many pathological conditions are accompanied by frequency of immunoglobulins with K light chain. The aim of this study was production of stable MoAb-se creating hybridoma with ability to secrete imunoglobulin specific for human K chain, immunochemical characterization of this MoAb and its possible application. Murine monoclonal antibody, as signed as MoAt 44, produced by hybridoma technology is specific for human kappa chain and fully immunochemically characterized. In this paper, we reported that MoAb 44 demonstrated excellent properties in most immunochemical techniques (immunoblot, dot-blot, immunofluorescence, double and radial immunodiffusion, immunoelectrophoresis...), which highly recommend this MoAb for the application as a tool for re search and immunodiagnostics.
AB  - Monoklonska antitela (MoAt) su sekretorni proizvodi kloniranih B ćelijskih limfoblastoidnih linija. Od poliklonskih antitela ih razlikuje: definisana specifičnost vezivanja, homogenost i mogućnost proizvodnje u velikoj količini. Budući da su brojna patološka stanja povezana sa sekrecijom imunoglobulina u čijem sastavu se nalazi kapa (k) tip lakog lanca, proizvodnja specifičnih monoklonskih antitela našla bi svoje mesto u njihovoj dijagnostici. Stoga je cilj našeg rada bio dobijanje stabilnog mišjeg hibridomskog klona koji bi sekretovao MoAt specifično za humani k lanac, imunohemijska karakterizacija tog MoAt i ispitivanje mogućnosti njegove primene. Mišje monoklonsko antitelo, označeno kao MoAt 44, proizvedeno hibridomskom tehnologijom, specifično je za k lanac humanih imunoglobulina i u potpunosti imunohemijski okarakterisano. MoAt 44 može da se koristi u većini imunohemijskih tehnika (imunoblot-u, dot-blot-u, imunofluorescenci, dvostrukoj i radijalnoj imunodifuziji, imunoelektroforezi...), što omogućava njegovu primenu u naučno-istraživačkom radu i imunodijagnostici.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities
T1  - Mišje monoklonsko antitelo specifično za kapa lanac humanih imunoglobulina i mogućnosti njegove primene
EP  - 25
IS  - 1
SP  - 21
VL  - 3
UR  - conv_65
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Šeatović, Saša S. and Živković, Irena and Jankov, Ratko and Živančević-Simonović, Snežana and Dimitrijević, Ljiljana",
year = "2002",
abstract = "Monoclonal antibodies (MoAb) are usually produced as the secretion products of cloned B lymphoblastoid cell lines. Advantages of MoAbs are that they could be selected to have desired specificity and produced in relatively large quantities of consistent quality and char acteristics. Interest in the production of re agents specific for human kappa (K) chain can be explained by the fact that many pathological conditions are accompanied by frequency of immunoglobulins with K light chain. The aim of this study was production of stable MoAb-se creating hybridoma with ability to secrete imunoglobulin specific for human K chain, immunochemical characterization of this MoAb and its possible application. Murine monoclonal antibody, as signed as MoAt 44, produced by hybridoma technology is specific for human kappa chain and fully immunochemically characterized. In this paper, we reported that MoAb 44 demonstrated excellent properties in most immunochemical techniques (immunoblot, dot-blot, immunofluorescence, double and radial immunodiffusion, immunoelectrophoresis...), which highly recommend this MoAb for the application as a tool for re search and immunodiagnostics., Monoklonska antitela (MoAt) su sekretorni proizvodi kloniranih B ćelijskih limfoblastoidnih linija. Od poliklonskih antitela ih razlikuje: definisana specifičnost vezivanja, homogenost i mogućnost proizvodnje u velikoj količini. Budući da su brojna patološka stanja povezana sa sekrecijom imunoglobulina u čijem sastavu se nalazi kapa (k) tip lakog lanca, proizvodnja specifičnih monoklonskih antitela našla bi svoje mesto u njihovoj dijagnostici. Stoga je cilj našeg rada bio dobijanje stabilnog mišjeg hibridomskog klona koji bi sekretovao MoAt specifično za humani k lanac, imunohemijska karakterizacija tog MoAt i ispitivanje mogućnosti njegove primene. Mišje monoklonsko antitelo, označeno kao MoAt 44, proizvedeno hibridomskom tehnologijom, specifično je za k lanac humanih imunoglobulina i u potpunosti imunohemijski okarakterisano. MoAt 44 može da se koristi u većini imunohemijskih tehnika (imunoblot-u, dot-blot-u, imunofluorescenci, dvostrukoj i radijalnoj imunodifuziji, imunoelektroforezi...), što omogućava njegovu primenu u naučno-istraživačkom radu i imunodijagnostici.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities, Mišje monoklonsko antitelo specifično za kapa lanac humanih imunoglobulina i mogućnosti njegove primene",
pages = "25-21",
number = "1",
volume = "3",
url = "conv_65"
}
Inić-Kanada, A., Stojanović, M., Šeatović, S. S., Živković, I., Jankov, R., Živančević-Simonović, S.,& Dimitrijević, L.. (2002). The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 3(1), 21-25.
conv_65
Inić-Kanada A, Stojanović M, Šeatović SS, Živković I, Jankov R, Živančević-Simonović S, Dimitrijević L. The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities. in Medicus. 2002;3(1):21-25.
conv_65 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Šeatović, Saša S., Živković, Irena, Jankov, Ratko, Živančević-Simonović, Snežana, Dimitrijević, Ljiljana, "The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities" in Medicus, 3, no. 1 (2002):21-25,
conv_65 .