Đergović-Petrović, Danica

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  • Đergović-Petrović, Danica (3)
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Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract

Ćirković-Veličković, Tanja; Polović, Natalija; Gavrović-Jankulović, Marija; Burazer, Lidija; Đergović-Petrović, Danica; Vučković, Olga; Drobnjak, Olika; Šporčić, Zorica; Atanasković-Marković, Marina; Jankov, Ratko

(Srpsko hemijsko društvo, Beograd, 2006)

TY  - JOUR
AU  - Ćirković-Veličković, Tanja
AU  - Polović, Natalija
AU  - Gavrović-Jankulović, Marija
AU  - Burazer, Lidija
AU  - Đergović-Petrović, Danica
AU  - Vučković, Olga
AU  - Drobnjak, Olika
AU  - Šporčić, Zorica
AU  - Atanasković-Marković, Marina
AU  - Jankov, Ratko
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/220
AB  - Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones.
AB  - U ovom radu su prikazani rezultati ispitivanja stabilnosti tri tipa alergoida polena pelina u simuliranom želudačnom soku. Koristeći kalijum-cijanat anhidrid ćilibarne i anhidrid maleinske kiseline, napravljeni su alergoidi polena pelina (Artemisia vulgaris). Saliva i želudačni sok su simulirani na osnovu evropske farmakopeje. Biohemijske i imunohemijske osobine derivata posle izlaganja različitim uslovima, praćene su: određivanjem broja slobodnih amino grupa u reakciji sa TNBS, SDS PAG elektroforezom, imunoblotom i određivanjem pelin-specifičnog imunoglobulina E (IgE). Izlaganje salivi u trajanju od 2 minuta ne utiče na biohemijske i imunohemijske osobine derivata. U kiseloj sredini želudačnog soka ne dolazi do značajnog demaleilovawa i desukcinilovanja. Čak i posle četvoročasovnog izlaganja, taj procenat je u opsegu 10-30 %. Alergoidi pelina se trenutno digestuju pepsinom, sa izuzetkom visoko rezistentne proteinske trake molekulske mase 28-35 kD, koja odgovara važnom IgE-vezujućem proteinu polena pelina. Imunoblotom i CAP-inhibicijom je pokazano da, u okviru fizioloških uslova, ne dolazi do stvaranja novih IgE-vezujućih epitopa. Hemijska stabilnost modifikovanih derivata u simuliranim uslovima želudačnog soka omogućuje da se tokom imunoterapije mogu primenjivati veće doze alergoida nego nemodifikovanog ekstrakta polena pelina.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract
T1  - Digestibilnost alergoida polena pelina u simuliranim uslovima gastrointestinalnog trakta
EP  - 888
IS  - 8-9
SP  - 879
VL  - 71
DO  - 10.2298/JSC0609879C
UR  - conv_19
ER  - 
@article{
author = "Ćirković-Veličković, Tanja and Polović, Natalija and Gavrović-Jankulović, Marija and Burazer, Lidija and Đergović-Petrović, Danica and Vučković, Olga and Drobnjak, Olika and Šporčić, Zorica and Atanasković-Marković, Marina and Jankov, Ratko",
year = "2006",
abstract = "Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones., U ovom radu su prikazani rezultati ispitivanja stabilnosti tri tipa alergoida polena pelina u simuliranom želudačnom soku. Koristeći kalijum-cijanat anhidrid ćilibarne i anhidrid maleinske kiseline, napravljeni su alergoidi polena pelina (Artemisia vulgaris). Saliva i želudačni sok su simulirani na osnovu evropske farmakopeje. Biohemijske i imunohemijske osobine derivata posle izlaganja različitim uslovima, praćene su: određivanjem broja slobodnih amino grupa u reakciji sa TNBS, SDS PAG elektroforezom, imunoblotom i određivanjem pelin-specifičnog imunoglobulina E (IgE). Izlaganje salivi u trajanju od 2 minuta ne utiče na biohemijske i imunohemijske osobine derivata. U kiseloj sredini želudačnog soka ne dolazi do značajnog demaleilovawa i desukcinilovanja. Čak i posle četvoročasovnog izlaganja, taj procenat je u opsegu 10-30 %. Alergoidi pelina se trenutno digestuju pepsinom, sa izuzetkom visoko rezistentne proteinske trake molekulske mase 28-35 kD, koja odgovara važnom IgE-vezujućem proteinu polena pelina. Imunoblotom i CAP-inhibicijom je pokazano da, u okviru fizioloških uslova, ne dolazi do stvaranja novih IgE-vezujućih epitopa. Hemijska stabilnost modifikovanih derivata u simuliranim uslovima želudačnog soka omogućuje da se tokom imunoterapije mogu primenjivati veće doze alergoida nego nemodifikovanog ekstrakta polena pelina.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract, Digestibilnost alergoida polena pelina u simuliranim uslovima gastrointestinalnog trakta",
pages = "888-879",
number = "8-9",
volume = "71",
doi = "10.2298/JSC0609879C",
url = "conv_19"
}
Ćirković-Veličković, T., Polović, N., Gavrović-Jankulović, M., Burazer, L., Đergović-Petrović, D., Vučković, O., Drobnjak, O., Šporčić, Z., Atanasković-Marković, M.,& Jankov, R.. (2006). Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 71(8-9), 879-888.
https://doi.org/10.2298/JSC0609879C
conv_19
Ćirković-Veličković T, Polović N, Gavrović-Jankulović M, Burazer L, Đergović-Petrović D, Vučković O, Drobnjak O, Šporčić Z, Atanasković-Marković M, Jankov R. Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract. in Journal of the Serbian Chemical Society. 2006;71(8-9):879-888.
doi:10.2298/JSC0609879C
conv_19 .
Ćirković-Veličković, Tanja, Polović, Natalija, Gavrović-Jankulović, Marija, Burazer, Lidija, Đergović-Petrović, Danica, Vučković, Olga, Drobnjak, Olika, Šporčić, Zorica, Atanasković-Marković, Marina, Jankov, Ratko, "Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract" in Journal of the Serbian Chemical Society, 71, no. 8-9 (2006):879-888,
https://doi.org/10.2298/JSC0609879C .,
conv_19 .
1

IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest

Polović, Natalija; Ćirković-Veličković, Tanja; Gavrović-Jankulović, Marija; Burazer, Lidija; Đergović-Petrović, Danica; Vučković, Olga; Jankov, Ratko

(Srpsko hemijsko društvo, Beograd, 2004)

TY  - JOUR
AU  - Polović, Natalija
AU  - Ćirković-Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Burazer, Lidija
AU  - Đergović-Petrović, Danica
AU  - Vučković, Olga
AU  - Jankov, Ratko
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/184
AB  - This study considers the influence of exposure to simulated gastrointestinal conditions (saliva, gut, intestine and acidic conditions of the gut) on IgG binding of unmodified allergens and three types of LMW allergoids of Artemisia vulgaris pollen extract obtained by means of potassium cyanate succinic and maleic anhydride. It also concerns the optimization of a self-developed ELISA assay for comparison of the specific IgG binding of mugwort pollen extract and modified mugwort pollen derivatives. The ELISA was conducted with a mugwort pollen extract coupled to the plate, using the sera from 12 mugwort- pollen allergic patients. The exposure to saliva fluid for 2 min did not influence the IgG binding properties of allergens and allergoids. Exposure of mugwort pollen allergens and LMW allergoids to the acidic conditions of the gut did not dramatically change their IgG binding properties. By exposing mugwort pollen extract and LMW derivatives to the SGF conditions for 1 h, the percent of IgG binding epitopes was reduced to a half of its starting value in the extract and to about 30%in all the allergoid samples. After prolonged exposure only the carbamyl derivative showed reduced IgG binding. Changes of the IgG binding potential of all four samples after exposure in SIF followed a similar pattern.
AB  - Predmet ovog rada je ispitivanje promena IgG vezivanja nemodifikovanih alergena polena A. vulgaris i tri tipa alergoida malih molekulskih masa dobijenih tretmanom sa kalijum-cijanatom, i anhidridima ćilibarne i meleinske posle izlaganja uslovima gastrointestinalnog trakta (saliva, želudac, tanko crevo i kisela sredina želudačnog soka) i optimizacija domaćeg ELISA testa za određivanje IgG vezivanja alergena i alergoida polena pelina.UELISA testu je za pločicu kuplovan ekstrakt polena pelina i korišćeni su serumi 12 pacijenata alergičnih na pelin. Izlaganje salivi u trajanju od 2 minuta ne utiče na IgG vezujuće osobine alergena i alergoida. Izlaganje kiseloj sredini želudačnog soka značajno ne utiče na IgG vezujuće osobine alergena i alergoida polena pelina. Posle izlaganja simuliranom želudačnom soku alergena i derivata u trajanju od 1 sata, procenat IgG vezujućih epitopa u nemodifikovanom uzorku se smanjuje na polovinu početne vrednosti i na oko 30 % kod sva tri derivata. Jedino se kod karbamil-derivata % IgG vezivanja dodatno smanjuje sa produžavanjem izlaganja SGF-u.Promene u IgG vezujućem potencijalu sva 4 uzorka posle izlaganja simuliranim uslovima tankog creva prate sličan obrazac.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest
T1  - Igg vezivanje alergena i alergoida polena pelina prethodno izloženih simuliranim uslovima gastrointestinalnog trakta mereno domaćim elisa testom
EP  - 540
IS  - 7
SP  - 533
VL  - 69
DO  - 10.2298/JSC0407533P
UR  - conv_18
ER  - 
@article{
author = "Polović, Natalija and Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija and Burazer, Lidija and Đergović-Petrović, Danica and Vučković, Olga and Jankov, Ratko",
year = "2004",
abstract = "This study considers the influence of exposure to simulated gastrointestinal conditions (saliva, gut, intestine and acidic conditions of the gut) on IgG binding of unmodified allergens and three types of LMW allergoids of Artemisia vulgaris pollen extract obtained by means of potassium cyanate succinic and maleic anhydride. It also concerns the optimization of a self-developed ELISA assay for comparison of the specific IgG binding of mugwort pollen extract and modified mugwort pollen derivatives. The ELISA was conducted with a mugwort pollen extract coupled to the plate, using the sera from 12 mugwort- pollen allergic patients. The exposure to saliva fluid for 2 min did not influence the IgG binding properties of allergens and allergoids. Exposure of mugwort pollen allergens and LMW allergoids to the acidic conditions of the gut did not dramatically change their IgG binding properties. By exposing mugwort pollen extract and LMW derivatives to the SGF conditions for 1 h, the percent of IgG binding epitopes was reduced to a half of its starting value in the extract and to about 30%in all the allergoid samples. After prolonged exposure only the carbamyl derivative showed reduced IgG binding. Changes of the IgG binding potential of all four samples after exposure in SIF followed a similar pattern., Predmet ovog rada je ispitivanje promena IgG vezivanja nemodifikovanih alergena polena A. vulgaris i tri tipa alergoida malih molekulskih masa dobijenih tretmanom sa kalijum-cijanatom, i anhidridima ćilibarne i meleinske posle izlaganja uslovima gastrointestinalnog trakta (saliva, želudac, tanko crevo i kisela sredina želudačnog soka) i optimizacija domaćeg ELISA testa za određivanje IgG vezivanja alergena i alergoida polena pelina.UELISA testu je za pločicu kuplovan ekstrakt polena pelina i korišćeni su serumi 12 pacijenata alergičnih na pelin. Izlaganje salivi u trajanju od 2 minuta ne utiče na IgG vezujuće osobine alergena i alergoida. Izlaganje kiseloj sredini želudačnog soka značajno ne utiče na IgG vezujuće osobine alergena i alergoida polena pelina. Posle izlaganja simuliranom želudačnom soku alergena i derivata u trajanju od 1 sata, procenat IgG vezujućih epitopa u nemodifikovanom uzorku se smanjuje na polovinu početne vrednosti i na oko 30 % kod sva tri derivata. Jedino se kod karbamil-derivata % IgG vezivanja dodatno smanjuje sa produžavanjem izlaganja SGF-u.Promene u IgG vezujućem potencijalu sva 4 uzorka posle izlaganja simuliranim uslovima tankog creva prate sličan obrazac.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest, Igg vezivanje alergena i alergoida polena pelina prethodno izloženih simuliranim uslovima gastrointestinalnog trakta mereno domaćim elisa testom",
pages = "540-533",
number = "7",
volume = "69",
doi = "10.2298/JSC0407533P",
url = "conv_18"
}
Polović, N., Ćirković-Veličković, T., Gavrović-Jankulović, M., Burazer, L., Đergović-Petrović, D., Vučković, O.,& Jankov, R.. (2004). IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 69(7), 533-540.
https://doi.org/10.2298/JSC0407533P
conv_18
Polović N, Ćirković-Veličković T, Gavrović-Jankulović M, Burazer L, Đergović-Petrović D, Vučković O, Jankov R. IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest. in Journal of the Serbian Chemical Society. 2004;69(7):533-540.
doi:10.2298/JSC0407533P
conv_18 .
Polović, Natalija, Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, Burazer, Lidija, Đergović-Petrović, Danica, Vučković, Olga, Jankov, Ratko, "IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest" in Journal of the Serbian Chemical Society, 69, no. 7 (2004):533-540,
https://doi.org/10.2298/JSC0407533P .,
conv_18 .
4
4
5

Somatostatin-14 alters relation between the thymocyte subpopulations in adult rats

Micić, M.; Đergović-Petrović, Danica; Rakin, Ana; Živković, Irena; Kosec, Duško

(Elsevier, Amsterdam, 2004)

TY  - CONF
AU  - Micić, M.
AU  - Đergović-Petrović, Danica
AU  - Rakin, Ana
AU  - Živković, Irena
AU  - Kosec, Duško
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/169
PB  - Elsevier, Amsterdam
C3  - Journal of Neuroimmunology
T1  - Somatostatin-14 alters relation between the thymocyte subpopulations in adult rats
EP  - 133
IS  - 1-2
SP  - 133
VL  - 154
UR  - conv_156
ER  - 
@conference{
author = "Micić, M. and Đergović-Petrović, Danica and Rakin, Ana and Živković, Irena and Kosec, Duško",
year = "2004",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Somatostatin-14 alters relation between the thymocyte subpopulations in adult rats",
pages = "133-133",
number = "1-2",
volume = "154",
url = "conv_156"
}
Micić, M., Đergović-Petrović, D., Rakin, A., Živković, I.,& Kosec, D.. (2004). Somatostatin-14 alters relation between the thymocyte subpopulations in adult rats. in Journal of Neuroimmunology
Elsevier, Amsterdam., 154(1-2), 133-133.
conv_156
Micić M, Đergović-Petrović D, Rakin A, Živković I, Kosec D. Somatostatin-14 alters relation between the thymocyte subpopulations in adult rats. in Journal of Neuroimmunology. 2004;154(1-2):133-133.
conv_156 .
Micić, M., Đergović-Petrović, Danica, Rakin, Ana, Živković, Irena, Kosec, Duško, "Somatostatin-14 alters relation between the thymocyte subpopulations in adult rats" in Journal of Neuroimmunology, 154, no. 1-2 (2004):133-133,
conv_156 .