Petrović, Spomenka

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Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract

Ćirković-Veličković, Tanja; Gavrović-Jankulović, Marija; Bukilica, Mirjana; Mandić, Ljuba M.; Petrović, Spomenka; Jankov, Ratko

(Srpsko hemijsko društvo, Beograd, 2002)

TY  - JOUR
AU  - Ćirković-Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Bukilica, Mirjana
AU  - Mandić, Ljuba M.
AU  - Petrović, Spomenka
AU  - Jankov, Ratko
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/140
AB  - An acid phosphatase from an extract of mugwort (Artemisia vulgaris) pollen was purified by a factor of 48 by a combination of ion exchange and gel-chromatography. The molecular weights of the enzyme were 76 kDa and 73 kDa, determined by gel filtration on a Sephadex G-100 sf column and by SDS PAGE(under reducing and non-reducing conditions), respectively. In analytical isoelectrofocusing, the enzyme appears as two very close bands pI at about 4.2. The optimum pH for the enzyme is 5.4. The apparent Km for p-nitrophenyl phosphate was estimated to be 0.16mM. The purified enzyme has broad specificity, and hydrolyses p-nitrophenyl phosphate and α-naphthyl phosphate. Pyrophosphate and O-phospho-L-tyrosine were estimated to be the best substrates for this enzyme as potential in vivo substrates. The enzyme is inhibited competitively by phosphate (Ki = 1.25 mM), molybdate (Ki = 0.055 mM) and pyrophosphate (Ki = 6.7 mM) and non-competitively by fluoride (Ki = 9.8 mM). Metal ions such as Hg2+, Cu2+ and Zn2+ express an inhibitory effect on the enzyme, while the enzyme is slightly activated by non-ionic detergents, Tween 20 and Triton X-100. There is no change in the enzyme activity in the presence of tartrate, citrate, EDTA, 1,10-phenanthroline and sulfhydryl-group modifiers such as p-chloromercuribenzoate and N-ethylmaleimide.
AB  - Kisela fosfataza ekstrakta polena visokog korova (Artemisia vulgaris) je prečišćena 48 puta kombinacijom jonoizmenjivačke i gel-hromatografije. Molekulska težina enzima je 76 kDa i 73 kDa, određena gel-filtracijom na matriksu Sephadex G-100 sf i SDS PAG elektroforezom (pri redukujućim i neredukujućim uslovima), respektivno. Pri izoelektrofokusiranju, enzim se sastoji iz dve vrlo bliske trake pI vrednosti oko 4,2. Optimalno pH za aktivnost enzima je 5,4. PrividnoKmza hidrolizu p-nitrofenil-fosfata je procenjeno da je 0,16 mM. Prečišćeni enzim ima široku specifičnost hidrolizuje p-nitrofenil-fosfat i α-naftil-fosfat. Pirofosfat i O-fosfo-L-tirozin su procenjeni kao najbolji od potencijalnih in vivo supstrata ovog enzima. Enzim je inhibiran kompetitivno fosfatom (Ki=1,25 mM), molibdatom (Ki=0,055 mM) i pirofosfatom (Ki=6,7 mM) a nekompetitivno fluoridom (Ki= 9,8mM). Joni metala, kao što su Hg2+, Cu2+ i Zn2+ iskazuju inhibitorni efekat na enzim, dok je efekat ne-jonskih detergenata, kao što su Tween 20 i Triton X-100 blago aktivirajuć i. Nema promene u aktivnosti enzima u prisustvu tartarata, citrata, EDTA 1,10-fenantrolina i modifikatora sulfhidrilnih grupa kao što su p-hloromerkuribenzoat i N-etilmaleimid.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract
T1  - Izolovanje i delimična karakterizacija kisele fosfataze ekstrakta polena Artemisia vulgaris
EP  - 572
IS  - 8-9
SP  - 567
VL  - 67
UR  - conv_17
ER  - 
@article{
author = "Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija and Bukilica, Mirjana and Mandić, Ljuba M. and Petrović, Spomenka and Jankov, Ratko",
year = "2002",
abstract = "An acid phosphatase from an extract of mugwort (Artemisia vulgaris) pollen was purified by a factor of 48 by a combination of ion exchange and gel-chromatography. The molecular weights of the enzyme were 76 kDa and 73 kDa, determined by gel filtration on a Sephadex G-100 sf column and by SDS PAGE(under reducing and non-reducing conditions), respectively. In analytical isoelectrofocusing, the enzyme appears as two very close bands pI at about 4.2. The optimum pH for the enzyme is 5.4. The apparent Km for p-nitrophenyl phosphate was estimated to be 0.16mM. The purified enzyme has broad specificity, and hydrolyses p-nitrophenyl phosphate and α-naphthyl phosphate. Pyrophosphate and O-phospho-L-tyrosine were estimated to be the best substrates for this enzyme as potential in vivo substrates. The enzyme is inhibited competitively by phosphate (Ki = 1.25 mM), molybdate (Ki = 0.055 mM) and pyrophosphate (Ki = 6.7 mM) and non-competitively by fluoride (Ki = 9.8 mM). Metal ions such as Hg2+, Cu2+ and Zn2+ express an inhibitory effect on the enzyme, while the enzyme is slightly activated by non-ionic detergents, Tween 20 and Triton X-100. There is no change in the enzyme activity in the presence of tartrate, citrate, EDTA, 1,10-phenanthroline and sulfhydryl-group modifiers such as p-chloromercuribenzoate and N-ethylmaleimide., Kisela fosfataza ekstrakta polena visokog korova (Artemisia vulgaris) je prečišćena 48 puta kombinacijom jonoizmenjivačke i gel-hromatografije. Molekulska težina enzima je 76 kDa i 73 kDa, određena gel-filtracijom na matriksu Sephadex G-100 sf i SDS PAG elektroforezom (pri redukujućim i neredukujućim uslovima), respektivno. Pri izoelektrofokusiranju, enzim se sastoji iz dve vrlo bliske trake pI vrednosti oko 4,2. Optimalno pH za aktivnost enzima je 5,4. PrividnoKmza hidrolizu p-nitrofenil-fosfata je procenjeno da je 0,16 mM. Prečišćeni enzim ima široku specifičnost hidrolizuje p-nitrofenil-fosfat i α-naftil-fosfat. Pirofosfat i O-fosfo-L-tirozin su procenjeni kao najbolji od potencijalnih in vivo supstrata ovog enzima. Enzim je inhibiran kompetitivno fosfatom (Ki=1,25 mM), molibdatom (Ki=0,055 mM) i pirofosfatom (Ki=6,7 mM) a nekompetitivno fluoridom (Ki= 9,8mM). Joni metala, kao što su Hg2+, Cu2+ i Zn2+ iskazuju inhibitorni efekat na enzim, dok je efekat ne-jonskih detergenata, kao što su Tween 20 i Triton X-100 blago aktivirajuć i. Nema promene u aktivnosti enzima u prisustvu tartarata, citrata, EDTA 1,10-fenantrolina i modifikatora sulfhidrilnih grupa kao što su p-hloromerkuribenzoat i N-etilmaleimid.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract, Izolovanje i delimična karakterizacija kisele fosfataze ekstrakta polena Artemisia vulgaris",
pages = "572-567",
number = "8-9",
volume = "67",
url = "conv_17"
}
Ćirković-Veličković, T., Gavrović-Jankulović, M., Bukilica, M., Mandić, L. M., Petrović, S.,& Jankov, R.. (2002). Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 67(8-9), 567-572.
conv_17
Ćirković-Veličković T, Gavrović-Jankulović M, Bukilica M, Mandić LM, Petrović S, Jankov R. Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract. in Journal of the Serbian Chemical Society. 2002;67(8-9):567-572.
conv_17 .
Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, Bukilica, Mirjana, Mandić, Ljuba M., Petrović, Spomenka, Jankov, Ratko, "Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract" in Journal of the Serbian Chemical Society, 67, no. 8-9 (2002):567-572,
conv_17 .

Skin reactivity and HEP system in pollen susceptible adults

Paranos, Svetlana; Petrović, Spomenka; Bojović, Ivanka

(Srpsko lekarsko društvo, Beograd, 2000)

TY  - JOUR
AU  - Paranos, Svetlana
AU  - Petrović, Spomenka
AU  - Bojović, Ivanka
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/120
AB  - INTRODUCTION Skin prick-test has been accepted as one of diagnostic criteria for atopyc diseases. In accordance with the recommendations of the EAACI (European Academy of Allergy and Clinical Immunology ), the Subcommittee for skin testing, allergen specific skin prick-test should be estimated in relation to positive control (histamine hydrochloride in the HEP system /histamine equivalent prick/) [1 -4]. The purpose of this study was to establish a recording system of skin sensitivity to allergen by using HEP method in our patients. MATERIAL AND METHODS The study was performed in 75 persons (49 females, 26 males, mean age 34.6 years) suffering from atopyc diseases (hay fever, allergic asthma or rhinoconjunctivitis) and susceptible to one of pollen allergens. Skin prick tests were performed and recorded as said before [2-6]. We used a house standardized (Torlak Institute) allergen solution of 5000 AU/mL (pollen of Dactylis glomerata, Lolium perennae, Phleum pratense), 7500 AU/mL (Phleum pratense) and 10 000 AU/mL (Phleum pratense) and histamine-hydrochloride solution (1 mg/mL and 10 mg/mL). Total and allergen specific serum IgE was made before the study by ELISA immunoassay (Pharmacia, Uppsala, EIA RAST Phadesim) in all selected persons and results were recorded in Phadebas RAST unit given by the manufacturer. According to the serum concentration of specific IgE, patients were classified into groups (low susceptibility, intermediate sensitivity and highly susceptible persons). Skin reactions were recorded according to histamine skin sensitivity (as reaction equal or larger in diameter than histamine papule). Additionally, measured allergenic and histamine wheal were sorted and frequency of positive tests was calculated. Skin testing was performed with the approval of the Ethic Committee of our Centre and with the written patients' consent. For statistical analysis we used x2 test, regression analysis and Kolmogar-Smirnov nonparametric test. Results for the confidence of 95% (p  lt  0.05), were considered significant. RESULTS In a group of 30 persons, susceptible to Phleum pratense pollen (Table 1, Graph 1 and 2), it was found that the number of positive tests was equally distributed among groups; it was dependent on the allergen concentrations, and was higher when using H1 (p  lt  0.01). When H10 was used as reference solution, only allergens of the highest concentration provided a significant number of positive tests (p  lt  0.01). There was no difference among groups of patients depending on the concentration of used histamine solution. In all 75 persons, we estimated skin-prick test performed by allergen solution of 5000 AU/mL and compared it with HI reaction. It was calculated that the most frequent histamine papule diameter was 3 mm and this value was farther used as the end-diameter in HEP system. Patients were classified in groups on the basis of serum IgE concentrations (RAST). The estimation of criterion positivity revealed the highest significance by using criterion Ap  gt  Hp (p  lt  0.001). At the same time, if the criterion Ap  gt  2Hp was used we were sure that 99% of our patients were highly sensitive to allergen we tested. DISCUSSION In the study it was established that allergen concentration of 5000 AU/mL and histamine solution of 1 mg/mL are sufficient to estimate skin-prick test in the HEP system. We found that the end diameter of histamine-papula was 3 mm. This finding suggests the good utility of HEP system. In addition, it has been proven that absolute value of papula diameter is not a critical parameter for the estimation of positivity or discrimation among patients regarding their susceptibility to allergen. By this system we found that criterion Ap  gt  Hp, provides a significantly higher number of positive tests (p  lt  0.001). The recommended criterion Ap  gt  Hp gives no discrimination among tested groups of patients, while using the criterion Ap  gt  2Hp we diagnosed highly susceptible persons (p  lt  0.01). The obtained results are in accordance with the recommendations of the EAACI Subcommittee, giving us the possibility of using a highly valide HEP system in our adult patients.
AB  - Kod osoba s atopijskom kostitucijom, testiranje kože ubodom ('prik-test') i rastvorom alergena, može se ocenjivati primenom sistema HEP (Histamine Equivalent Prick), što znači reaktivnost kože na test s alergenom jednaka reaktivnosti kože na test s histaminom. Prethodno, međutim, treba utvrditi najmanje koncentracije rastvora histamina i alergena koje izazivaju reakciju kože pri testu ubodom kod najvećeg broja preosetljivih osoba. Zatim, utvrditi koja je najčešća veličina reaktivnosti kože na histamin kod preosetljivih osoba u odnosu na koju se ocenjuje test kože s rastvorom alergena. Kod 75 osoba s jednom od atopijskih bolesti i dokazanim antitelima IgE, specifičnim za polene u serumu (RAST, Pharmacia), testirali smo kožu (ubodom) alergenima polena trava. Rezultate testa smo ocenjivali prema reaktivnosti kože na test s histamiom. Od testiranih 75 osoba, kod 30 je izvršen test s tri različite koncentracije alergena (polena trave Phleum pratense) i dve različčite koncentracije rastvora histamina (od 1 mg/mL i 10 mg/mL). Utvrdili smo da je broj pozitivno ocenjenih rezultata testiranja kože srazmeran koncentraciji primenjenog rastvora alergena, a obrnuto srazmeran koncentraciji rastvora histamina. Najčešća veličina prečnika histaminske papule je 3 mm (rastvor histamina koncentracije 1 mg/mL) i ne zavisi od preosetljivosti bolesnika na alergen. Utvrđeno je da veličina reakcije kože (papula) izazvana alergenom (koncentracija 5000 AJ/mL), koja je veća od histaminske papule, ukazuje sa sigurnošću od 99,9 posto (r  lt  0,001) da je ispitivana osoba preosetljiva. Ako je alergenska papula dvostruko veća od histaminske papule ili još veća, reč je o visokopreosetljivoj osobi (r  lt  0,01). U graničnim slučajevima treba primeniti rastvor alergena od 7500 AJ/mL.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Skin reactivity and HEP system in pollen susceptible adults
T1  - Reaktivnost kože i sistem 'prik-test jednak histaminskom' kod osoba preosetljivih na polene trava
EP  - 199
IS  - 5-6
SP  - 194
VL  - 128
UR  - conv_37
ER  - 
@article{
author = "Paranos, Svetlana and Petrović, Spomenka and Bojović, Ivanka",
year = "2000",
abstract = "INTRODUCTION Skin prick-test has been accepted as one of diagnostic criteria for atopyc diseases. In accordance with the recommendations of the EAACI (European Academy of Allergy and Clinical Immunology ), the Subcommittee for skin testing, allergen specific skin prick-test should be estimated in relation to positive control (histamine hydrochloride in the HEP system /histamine equivalent prick/) [1 -4]. The purpose of this study was to establish a recording system of skin sensitivity to allergen by using HEP method in our patients. MATERIAL AND METHODS The study was performed in 75 persons (49 females, 26 males, mean age 34.6 years) suffering from atopyc diseases (hay fever, allergic asthma or rhinoconjunctivitis) and susceptible to one of pollen allergens. Skin prick tests were performed and recorded as said before [2-6]. We used a house standardized (Torlak Institute) allergen solution of 5000 AU/mL (pollen of Dactylis glomerata, Lolium perennae, Phleum pratense), 7500 AU/mL (Phleum pratense) and 10 000 AU/mL (Phleum pratense) and histamine-hydrochloride solution (1 mg/mL and 10 mg/mL). Total and allergen specific serum IgE was made before the study by ELISA immunoassay (Pharmacia, Uppsala, EIA RAST Phadesim) in all selected persons and results were recorded in Phadebas RAST unit given by the manufacturer. According to the serum concentration of specific IgE, patients were classified into groups (low susceptibility, intermediate sensitivity and highly susceptible persons). Skin reactions were recorded according to histamine skin sensitivity (as reaction equal or larger in diameter than histamine papule). Additionally, measured allergenic and histamine wheal were sorted and frequency of positive tests was calculated. Skin testing was performed with the approval of the Ethic Committee of our Centre and with the written patients' consent. For statistical analysis we used x2 test, regression analysis and Kolmogar-Smirnov nonparametric test. Results for the confidence of 95% (p  lt  0.05), were considered significant. RESULTS In a group of 30 persons, susceptible to Phleum pratense pollen (Table 1, Graph 1 and 2), it was found that the number of positive tests was equally distributed among groups; it was dependent on the allergen concentrations, and was higher when using H1 (p  lt  0.01). When H10 was used as reference solution, only allergens of the highest concentration provided a significant number of positive tests (p  lt  0.01). There was no difference among groups of patients depending on the concentration of used histamine solution. In all 75 persons, we estimated skin-prick test performed by allergen solution of 5000 AU/mL and compared it with HI reaction. It was calculated that the most frequent histamine papule diameter was 3 mm and this value was farther used as the end-diameter in HEP system. Patients were classified in groups on the basis of serum IgE concentrations (RAST). The estimation of criterion positivity revealed the highest significance by using criterion Ap  gt  Hp (p  lt  0.001). At the same time, if the criterion Ap  gt  2Hp was used we were sure that 99% of our patients were highly sensitive to allergen we tested. DISCUSSION In the study it was established that allergen concentration of 5000 AU/mL and histamine solution of 1 mg/mL are sufficient to estimate skin-prick test in the HEP system. We found that the end diameter of histamine-papula was 3 mm. This finding suggests the good utility of HEP system. In addition, it has been proven that absolute value of papula diameter is not a critical parameter for the estimation of positivity or discrimation among patients regarding their susceptibility to allergen. By this system we found that criterion Ap  gt  Hp, provides a significantly higher number of positive tests (p  lt  0.001). The recommended criterion Ap  gt  Hp gives no discrimination among tested groups of patients, while using the criterion Ap  gt  2Hp we diagnosed highly susceptible persons (p  lt  0.01). The obtained results are in accordance with the recommendations of the EAACI Subcommittee, giving us the possibility of using a highly valide HEP system in our adult patients., Kod osoba s atopijskom kostitucijom, testiranje kože ubodom ('prik-test') i rastvorom alergena, može se ocenjivati primenom sistema HEP (Histamine Equivalent Prick), što znači reaktivnost kože na test s alergenom jednaka reaktivnosti kože na test s histaminom. Prethodno, međutim, treba utvrditi najmanje koncentracije rastvora histamina i alergena koje izazivaju reakciju kože pri testu ubodom kod najvećeg broja preosetljivih osoba. Zatim, utvrditi koja je najčešća veličina reaktivnosti kože na histamin kod preosetljivih osoba u odnosu na koju se ocenjuje test kože s rastvorom alergena. Kod 75 osoba s jednom od atopijskih bolesti i dokazanim antitelima IgE, specifičnim za polene u serumu (RAST, Pharmacia), testirali smo kožu (ubodom) alergenima polena trava. Rezultate testa smo ocenjivali prema reaktivnosti kože na test s histamiom. Od testiranih 75 osoba, kod 30 je izvršen test s tri različite koncentracije alergena (polena trave Phleum pratense) i dve različčite koncentracije rastvora histamina (od 1 mg/mL i 10 mg/mL). Utvrdili smo da je broj pozitivno ocenjenih rezultata testiranja kože srazmeran koncentraciji primenjenog rastvora alergena, a obrnuto srazmeran koncentraciji rastvora histamina. Najčešća veličina prečnika histaminske papule je 3 mm (rastvor histamina koncentracije 1 mg/mL) i ne zavisi od preosetljivosti bolesnika na alergen. Utvrđeno je da veličina reakcije kože (papula) izazvana alergenom (koncentracija 5000 AJ/mL), koja je veća od histaminske papule, ukazuje sa sigurnošću od 99,9 posto (r  lt  0,001) da je ispitivana osoba preosetljiva. Ako je alergenska papula dvostruko veća od histaminske papule ili još veća, reč je o visokopreosetljivoj osobi (r  lt  0,01). U graničnim slučajevima treba primeniti rastvor alergena od 7500 AJ/mL.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Skin reactivity and HEP system in pollen susceptible adults, Reaktivnost kože i sistem 'prik-test jednak histaminskom' kod osoba preosetljivih na polene trava",
pages = "199-194",
number = "5-6",
volume = "128",
url = "conv_37"
}
Paranos, S., Petrović, S.,& Bojović, I.. (2000). Skin reactivity and HEP system in pollen susceptible adults. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 128(5-6), 194-199.
conv_37
Paranos S, Petrović S, Bojović I. Skin reactivity and HEP system in pollen susceptible adults. in Srpski arhiv za celokupno lekarstvo. 2000;128(5-6):194-199.
conv_37 .
Paranos, Svetlana, Petrović, Spomenka, Bojović, Ivanka, "Skin reactivity and HEP system in pollen susceptible adults" in Srpski arhiv za celokupno lekarstvo, 128, no. 5-6 (2000):194-199,
conv_37 .

Isolation and partial characterization of Fes p 4 allergen

Gavrović-Jankulović, Marija; Ćirković, Tanja; Bukilica, Mirjana; Fahlbusch, B.; Petrović, Spomenka; Jankov, Ratko

(2000)

TY  - JOUR
AU  - Gavrović-Jankulović, Marija
AU  - Ćirković, Tanja
AU  - Bukilica, Mirjana
AU  - Fahlbusch, B.
AU  - Petrović, Spomenka
AU  - Jankov, Ratko
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/116
AB  - More than 75% of grass pollen-allergic patients produce specific IgE antibodies against group-4 allergens. Purification and characterization of different grass group-4 allergens should help to further understand their allergenicity. In this study, an attempt was made to isolate and characterize Fes p 4 allergen by several biochemical and immunochemical methods. Fes p 4 was purified by a combination of chromatographic techniques (gel permeation and ion exchange chromatography). Isolated protein revealed four main spots at a molecular weight of 60 kDa and a pl ranging from 8.7 to 9.1. Eight sera were selected from patients with positive result of skin prick test to the mixture of grass pollen extracts. ELISA inhibition technique was used to study Fes p 4-specific IgE in the patients' sera. ELISA to Festuca pratensis was inhibited up to 80% by F. pratensis pollen extract and up to 48% by Fes p 4. 2D-PAGE-immunoblot was used to identify allergenic and antigenic components of Fes p 4 with patients' IgE and monoclonal antibodies (MABs). Three components of purified protein expressed IgE binding ability. Two MABs which recognized unrelated regions on Phl p 4, bound three components of Fes p 4. The role of the carbohydrate moiety in allergenicity was examined with individual patient sera by using periodate-treated Fes p 4. Six out of eight patients reduced IgE binding to periodate-treated allergen. Isolated Fes p 4 glycoprotein consisted of four components, three of which were allergenic, and share common epitopes specific for grass group-4 homologs. The results of periodate oxidation of Fes p 4 suggest that the carbohydrate moiety is involved in IgE binding.
T2  - Journal of Investigational Allergology and Clinical Immunology
T1  - Isolation and partial characterization of Fes p 4 allergen
EP  - 367
IS  - 6
SP  - 361
VL  - 10
UR  - conv_526
ER  - 
@article{
author = "Gavrović-Jankulović, Marija and Ćirković, Tanja and Bukilica, Mirjana and Fahlbusch, B. and Petrović, Spomenka and Jankov, Ratko",
year = "2000",
abstract = "More than 75% of grass pollen-allergic patients produce specific IgE antibodies against group-4 allergens. Purification and characterization of different grass group-4 allergens should help to further understand their allergenicity. In this study, an attempt was made to isolate and characterize Fes p 4 allergen by several biochemical and immunochemical methods. Fes p 4 was purified by a combination of chromatographic techniques (gel permeation and ion exchange chromatography). Isolated protein revealed four main spots at a molecular weight of 60 kDa and a pl ranging from 8.7 to 9.1. Eight sera were selected from patients with positive result of skin prick test to the mixture of grass pollen extracts. ELISA inhibition technique was used to study Fes p 4-specific IgE in the patients' sera. ELISA to Festuca pratensis was inhibited up to 80% by F. pratensis pollen extract and up to 48% by Fes p 4. 2D-PAGE-immunoblot was used to identify allergenic and antigenic components of Fes p 4 with patients' IgE and monoclonal antibodies (MABs). Three components of purified protein expressed IgE binding ability. Two MABs which recognized unrelated regions on Phl p 4, bound three components of Fes p 4. The role of the carbohydrate moiety in allergenicity was examined with individual patient sera by using periodate-treated Fes p 4. Six out of eight patients reduced IgE binding to periodate-treated allergen. Isolated Fes p 4 glycoprotein consisted of four components, three of which were allergenic, and share common epitopes specific for grass group-4 homologs. The results of periodate oxidation of Fes p 4 suggest that the carbohydrate moiety is involved in IgE binding.",
journal = "Journal of Investigational Allergology and Clinical Immunology",
title = "Isolation and partial characterization of Fes p 4 allergen",
pages = "367-361",
number = "6",
volume = "10",
url = "conv_526"
}
Gavrović-Jankulović, M., Ćirković, T., Bukilica, M., Fahlbusch, B., Petrović, S.,& Jankov, R.. (2000). Isolation and partial characterization of Fes p 4 allergen. in Journal of Investigational Allergology and Clinical Immunology, 10(6), 361-367.
conv_526
Gavrović-Jankulović M, Ćirković T, Bukilica M, Fahlbusch B, Petrović S, Jankov R. Isolation and partial characterization of Fes p 4 allergen. in Journal of Investigational Allergology and Clinical Immunology. 2000;10(6):361-367.
conv_526 .
Gavrović-Jankulović, Marija, Ćirković, Tanja, Bukilica, Mirjana, Fahlbusch, B., Petrović, Spomenka, Jankov, Ratko, "Isolation and partial characterization of Fes p 4 allergen" in Journal of Investigational Allergology and Clinical Immunology, 10, no. 6 (2000):361-367,
conv_526 .
15

Early effects of rush immunotherapy with Dermatophagoides pteronyssinus in asthmatics

Paranos, Svetlana; Petrović, Spomenka

(1997)

TY  - JOUR
AU  - Paranos, Svetlana
AU  - Petrović, Spomenka
PY  - 1997
UR  - http://intor.torlakinstitut.com/handle/123456789/73
AB  - In this study we assessed the effects of Dermatophagoides pteronyssinus (Dpt) rush immunotherapy in comparison with placebo treatment in our asthmatic patients. Fourteen highly Dpt-susceptible adults were randomized in two groups (immunotherapy and placebo) and treated in single-blind manner. Patients were selected according to the recommendation of the immunotherapy position paper (1993). To minimize side effects we modified the protocol by adjusting allergen doses for each patient separately. Immunologic (total and Dpt-specific serum IgE and IgG antibodies/EIA, Pharmacia) and clinical parameters (spirometry, medication score and skin testing) were recorded before treatment, after 2 weeks, at the second month and after 4 months of immunotherapy onset. None of the patients had life-threatening side effects in the course of the treatment. The results obtained demonstrated significant influence of immunotherapy on Dpt-specific serum IgG synthesis (Kruskal Wallis test, p < 0.05) and on the late phase skin reaction with Dpt (U-test, p < 0.05) at the end of the second month of immunotherapy onset. In the immunotherapy group, we also registered a negative correlation between concentrations of Dpt-specific serum IgE and IgG antibodies (p = -0.83; p < 0.05), at the end of the second month. In addition, diversities among patients, expressed by immunologic parameters, were related to the amount of delivered allergen. There were no significant differences between groups concerning medication score from opposite to better FEV1, PEFR and dPEFR results (Kruskal Wallis, p < 0.05) in the placebo group. In conclusion, Dpt-specific serum IgG concentration, immunologic score and late phase skin reactivity to allergen appeared to be the valid parameters of rush-immunotherapy achievement, while delivered allergen dose also seemed to be an influencing factor.
T2  - Journal of Investigational Allergology and Clinical Immunology
T1  - Early effects of rush immunotherapy with Dermatophagoides pteronyssinus in asthmatics
EP  - 595
IS  - 6
SP  - 588
VL  - 7
UR  - conv_539
ER  - 
@article{
author = "Paranos, Svetlana and Petrović, Spomenka",
year = "1997",
abstract = "In this study we assessed the effects of Dermatophagoides pteronyssinus (Dpt) rush immunotherapy in comparison with placebo treatment in our asthmatic patients. Fourteen highly Dpt-susceptible adults were randomized in two groups (immunotherapy and placebo) and treated in single-blind manner. Patients were selected according to the recommendation of the immunotherapy position paper (1993). To minimize side effects we modified the protocol by adjusting allergen doses for each patient separately. Immunologic (total and Dpt-specific serum IgE and IgG antibodies/EIA, Pharmacia) and clinical parameters (spirometry, medication score and skin testing) were recorded before treatment, after 2 weeks, at the second month and after 4 months of immunotherapy onset. None of the patients had life-threatening side effects in the course of the treatment. The results obtained demonstrated significant influence of immunotherapy on Dpt-specific serum IgG synthesis (Kruskal Wallis test, p < 0.05) and on the late phase skin reaction with Dpt (U-test, p < 0.05) at the end of the second month of immunotherapy onset. In the immunotherapy group, we also registered a negative correlation between concentrations of Dpt-specific serum IgE and IgG antibodies (p = -0.83; p < 0.05), at the end of the second month. In addition, diversities among patients, expressed by immunologic parameters, were related to the amount of delivered allergen. There were no significant differences between groups concerning medication score from opposite to better FEV1, PEFR and dPEFR results (Kruskal Wallis, p < 0.05) in the placebo group. In conclusion, Dpt-specific serum IgG concentration, immunologic score and late phase skin reactivity to allergen appeared to be the valid parameters of rush-immunotherapy achievement, while delivered allergen dose also seemed to be an influencing factor.",
journal = "Journal of Investigational Allergology and Clinical Immunology",
title = "Early effects of rush immunotherapy with Dermatophagoides pteronyssinus in asthmatics",
pages = "595-588",
number = "6",
volume = "7",
url = "conv_539"
}
Paranos, S.,& Petrović, S.. (1997). Early effects of rush immunotherapy with Dermatophagoides pteronyssinus in asthmatics. in Journal of Investigational Allergology and Clinical Immunology, 7(6), 588-595.
conv_539
Paranos S, Petrović S. Early effects of rush immunotherapy with Dermatophagoides pteronyssinus in asthmatics. in Journal of Investigational Allergology and Clinical Immunology. 1997;7(6):588-595.
conv_539 .
Paranos, Svetlana, Petrović, Spomenka, "Early effects of rush immunotherapy with Dermatophagoides pteronyssinus in asthmatics" in Journal of Investigational Allergology and Clinical Immunology, 7, no. 6 (1997):588-595,
conv_539 .
8

Mixed antiglobulin reaction as a screening test for the diagnosis of male immunological infertility

Vučković, Olga; Dakić, R.; Micić, S.; Petrović, Spomenka

(Hrvatsko prirodoslovno društvo, Zagreb, 1990)

TY  - CONF
AU  - Vučković, Olga
AU  - Dakić, R.
AU  - Micić, S.
AU  - Petrović, Spomenka
PY  - 1990
UR  - http://intor.torlakinstitut.com/handle/123456789/47
PB  - Hrvatsko prirodoslovno društvo, Zagreb
C3  - Periodicum Biologorum
T1  - Mixed antiglobulin reaction as a screening test for the diagnosis of male immunological infertility
IS  - 1
SP  - 47
VL  - 92
UR  - conv_608
ER  - 
@conference{
author = "Vučković, Olga and Dakić, R. and Micić, S. and Petrović, Spomenka",
year = "1990",
publisher = "Hrvatsko prirodoslovno društvo, Zagreb",
journal = "Periodicum Biologorum",
title = "Mixed antiglobulin reaction as a screening test for the diagnosis of male immunological infertility",
number = "1",
pages = "47",
volume = "92",
url = "conv_608"
}
Vučković, O., Dakić, R., Micić, S.,& Petrović, S.. (1990). Mixed antiglobulin reaction as a screening test for the diagnosis of male immunological infertility. in Periodicum Biologorum
Hrvatsko prirodoslovno društvo, Zagreb., 92(1), 47.
conv_608
Vučković O, Dakić R, Micić S, Petrović S. Mixed antiglobulin reaction as a screening test for the diagnosis of male immunological infertility. in Periodicum Biologorum. 1990;92(1):47.
conv_608 .
Vučković, Olga, Dakić, R., Micić, S., Petrović, Spomenka, "Mixed antiglobulin reaction as a screening test for the diagnosis of male immunological infertility" in Periodicum Biologorum, 92, no. 1 (1990):47,
conv_608 .