Bukilica, Mirjana

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Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases

Kostić, Irena; Živančević-Simonović, Snežana; Bukilica, Mirjana; Dimitrijević, Ljiljana

(Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac, 2006)

TY  - JOUR
AU  - Kostić, Irena
AU  - Živančević-Simonović, Snežana
AU  - Bukilica, Mirjana
AU  - Dimitrijević, Ljiljana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/221
AB  - Autoimmune thyroid disease (ATD) has been described in patients with connective tissue diseases (CTD). The aim of this study was to estimate and compare the prevalence of ATD in a group of 91 CTD patients, and in their subgroups: 53 systemic lupus erythematosus (SLE), 24 rheumatoid arthritis (RA), 7 primary Sjogren’s syndrome (SSy) and 7 progressive systemic sclerosis (SSc) patients. A control group of 34 healthy blood volunteers was used for comparison. Serum levels of free thyroxine (FT4), thyroid stimulating hormone (TSH), as well as thyroid autoantibodies (Abs) specific of thyroperoxidase (TPO) and thyroglobulin (TG) were examined. CTD patients, in general, as well as SLE and RA subgroups, had significantly higher number of thyroid dysfunction than the control group (p lt 0.05). The most prominent thyroid dysfunction was subclinical hypothyroidism, with a higher prevalence in all subgroups of patients when compared to the control. Anti-TPO Abs were detected in a significant number of CTD patients, especially in SLE subgroup when compared to the control group. It was also found that a higher number of CTD patients, SLE and RA subgroups, had positive anti-Tg Abs, when compared to the control subjects. In conclusion, the prevalence of ATD in CTD patients was more frequent than in the control group. The patients with anti-TPO Abs and anti-Tg Abs at the time when they were analyzed, had hyperthyroidism, hypothyroidism or were clinically and biochemically euthyroid. The prevalence of hypothyroidism was greater than the prevalence of hyperthyroidism in all subgroups of patients.
AB  - Autoimunske bolesti štitaste žlezde (AITD) opisane su kod bolesnika sa sistemskim bolestima vezivnog tkiva (SBVT). Cilj ovog rada bio je da se ispita prevalencija AITD u grupi od 91 bolesnika sa SBVT, koja je uključila 53 bolesnika sa sistemskim eritemskim lupusom (SLE), 24 obolelih sa reumatoidnim artritisom (RA) i po 7 obolelih od primarnog Sjogrenovog sindroma (SSy) i progresivne sistemske skleroze (SSc). Kontrolnu grupu ispitanika činila su 34 dobrovoljna davaoca krvi. Kod svih učesnika u studiji merene su serumske koncentracije slobodnog tiroksina (FT4), tireostimulišućeg hormona (TSH), kao i autoantitela specifičnih za tireoperoksidazu (anti-TPO At) i tireoglobulin (anti-Tg At). U grupi bolesnika sa SBVT, kao i u podgrupama obolelih od SLE i RA, nađena je statistički značajno veća učestalost poremećaja funkcije štitaste žlezde u odnosu na kontrolnu grupu (p lt 0.05), a od svih poremećaja funkcije štitaste žlezde najčečće je detektovana subklinička hipotireoza. Anti-TPO At nađena su kod značajno većeg broja bolesnika sa SBVT i SLE, u odnosu na kontrolnu grupu. Isto tako, i anti-Tg At su detektovana kod većeg broja ispitanika u grupi SBVT, i podgrupama SLE i RA, nego kod zdravih osoba. U zaključku, prevalencija AITD kod obolelih od SBVT veća je nego u kontrolnoj grupi. Bolesnici sa anti- TPO At i anti-Tg At u vreme kada su analizirani, imali su subkliničku ili klinički manifestnu hipotireozu ili hipertireozu, ili su još bili u stadijumu bolesti u kome se ne može detektovati poremećaj funkcije štitaste žlezde. Prevalencija hipotireoze bila je veća od prevalencije hipertireoze u svim podgrupama bolesnika sa SBVT.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases
T1  - Funkcija štitaste žlezde i prisustvo antitireoidnih autoantitela kod bolesnika sa sistemskim bolestima vezivnog tkiva
EP  - 64
IS  - 2
SP  - 61
VL  - 7
UR  - conv_70
ER  - 
@article{
author = "Kostić, Irena and Živančević-Simonović, Snežana and Bukilica, Mirjana and Dimitrijević, Ljiljana",
year = "2006",
abstract = "Autoimmune thyroid disease (ATD) has been described in patients with connective tissue diseases (CTD). The aim of this study was to estimate and compare the prevalence of ATD in a group of 91 CTD patients, and in their subgroups: 53 systemic lupus erythematosus (SLE), 24 rheumatoid arthritis (RA), 7 primary Sjogren’s syndrome (SSy) and 7 progressive systemic sclerosis (SSc) patients. A control group of 34 healthy blood volunteers was used for comparison. Serum levels of free thyroxine (FT4), thyroid stimulating hormone (TSH), as well as thyroid autoantibodies (Abs) specific of thyroperoxidase (TPO) and thyroglobulin (TG) were examined. CTD patients, in general, as well as SLE and RA subgroups, had significantly higher number of thyroid dysfunction than the control group (p lt 0.05). The most prominent thyroid dysfunction was subclinical hypothyroidism, with a higher prevalence in all subgroups of patients when compared to the control. Anti-TPO Abs were detected in a significant number of CTD patients, especially in SLE subgroup when compared to the control group. It was also found that a higher number of CTD patients, SLE and RA subgroups, had positive anti-Tg Abs, when compared to the control subjects. In conclusion, the prevalence of ATD in CTD patients was more frequent than in the control group. The patients with anti-TPO Abs and anti-Tg Abs at the time when they were analyzed, had hyperthyroidism, hypothyroidism or were clinically and biochemically euthyroid. The prevalence of hypothyroidism was greater than the prevalence of hyperthyroidism in all subgroups of patients., Autoimunske bolesti štitaste žlezde (AITD) opisane su kod bolesnika sa sistemskim bolestima vezivnog tkiva (SBVT). Cilj ovog rada bio je da se ispita prevalencija AITD u grupi od 91 bolesnika sa SBVT, koja je uključila 53 bolesnika sa sistemskim eritemskim lupusom (SLE), 24 obolelih sa reumatoidnim artritisom (RA) i po 7 obolelih od primarnog Sjogrenovog sindroma (SSy) i progresivne sistemske skleroze (SSc). Kontrolnu grupu ispitanika činila su 34 dobrovoljna davaoca krvi. Kod svih učesnika u studiji merene su serumske koncentracije slobodnog tiroksina (FT4), tireostimulišućeg hormona (TSH), kao i autoantitela specifičnih za tireoperoksidazu (anti-TPO At) i tireoglobulin (anti-Tg At). U grupi bolesnika sa SBVT, kao i u podgrupama obolelih od SLE i RA, nađena je statistički značajno veća učestalost poremećaja funkcije štitaste žlezde u odnosu na kontrolnu grupu (p lt 0.05), a od svih poremećaja funkcije štitaste žlezde najčečće je detektovana subklinička hipotireoza. Anti-TPO At nađena su kod značajno većeg broja bolesnika sa SBVT i SLE, u odnosu na kontrolnu grupu. Isto tako, i anti-Tg At su detektovana kod većeg broja ispitanika u grupi SBVT, i podgrupama SLE i RA, nego kod zdravih osoba. U zaključku, prevalencija AITD kod obolelih od SBVT veća je nego u kontrolnoj grupi. Bolesnici sa anti- TPO At i anti-Tg At u vreme kada su analizirani, imali su subkliničku ili klinički manifestnu hipotireozu ili hipertireozu, ili su još bili u stadijumu bolesti u kome se ne može detektovati poremećaj funkcije štitaste žlezde. Prevalencija hipotireoze bila je veća od prevalencije hipertireoze u svim podgrupama bolesnika sa SBVT.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases, Funkcija štitaste žlezde i prisustvo antitireoidnih autoantitela kod bolesnika sa sistemskim bolestima vezivnog tkiva",
pages = "64-61",
number = "2",
volume = "7",
url = "conv_70"
}
Kostić, I., Živančević-Simonović, S., Bukilica, M.,& Dimitrijević, L.. (2006). Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 7(2), 61-64.
conv_70
Kostić I, Živančević-Simonović S, Bukilica M, Dimitrijević L. Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases. in Medicus. 2006;7(2):61-64.
conv_70 .
Kostić, Irena, Živančević-Simonović, Snežana, Bukilica, Mirjana, Dimitrijević, Ljiljana, "Thyroid function and antithyroid autoantibodies in patients with connective tissue diseases" in Medicus, 7, no. 2 (2006):61-64,
conv_70 .
3

Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract

Ćirković-Veličković, Tanja; Gavrović-Jankulović, Marija; Bukilica, Mirjana; Mandić, Ljuba M.; Petrović, Spomenka; Jankov, Ratko

(Srpsko hemijsko društvo, Beograd, 2002)

TY  - JOUR
AU  - Ćirković-Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Bukilica, Mirjana
AU  - Mandić, Ljuba M.
AU  - Petrović, Spomenka
AU  - Jankov, Ratko
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/140
AB  - An acid phosphatase from an extract of mugwort (Artemisia vulgaris) pollen was purified by a factor of 48 by a combination of ion exchange and gel-chromatography. The molecular weights of the enzyme were 76 kDa and 73 kDa, determined by gel filtration on a Sephadex G-100 sf column and by SDS PAGE(under reducing and non-reducing conditions), respectively. In analytical isoelectrofocusing, the enzyme appears as two very close bands pI at about 4.2. The optimum pH for the enzyme is 5.4. The apparent Km for p-nitrophenyl phosphate was estimated to be 0.16mM. The purified enzyme has broad specificity, and hydrolyses p-nitrophenyl phosphate and α-naphthyl phosphate. Pyrophosphate and O-phospho-L-tyrosine were estimated to be the best substrates for this enzyme as potential in vivo substrates. The enzyme is inhibited competitively by phosphate (Ki = 1.25 mM), molybdate (Ki = 0.055 mM) and pyrophosphate (Ki = 6.7 mM) and non-competitively by fluoride (Ki = 9.8 mM). Metal ions such as Hg2+, Cu2+ and Zn2+ express an inhibitory effect on the enzyme, while the enzyme is slightly activated by non-ionic detergents, Tween 20 and Triton X-100. There is no change in the enzyme activity in the presence of tartrate, citrate, EDTA, 1,10-phenanthroline and sulfhydryl-group modifiers such as p-chloromercuribenzoate and N-ethylmaleimide.
AB  - Kisela fosfataza ekstrakta polena visokog korova (Artemisia vulgaris) je prečišćena 48 puta kombinacijom jonoizmenjivačke i gel-hromatografije. Molekulska težina enzima je 76 kDa i 73 kDa, određena gel-filtracijom na matriksu Sephadex G-100 sf i SDS PAG elektroforezom (pri redukujućim i neredukujućim uslovima), respektivno. Pri izoelektrofokusiranju, enzim se sastoji iz dve vrlo bliske trake pI vrednosti oko 4,2. Optimalno pH za aktivnost enzima je 5,4. PrividnoKmza hidrolizu p-nitrofenil-fosfata je procenjeno da je 0,16 mM. Prečišćeni enzim ima široku specifičnost hidrolizuje p-nitrofenil-fosfat i α-naftil-fosfat. Pirofosfat i O-fosfo-L-tirozin su procenjeni kao najbolji od potencijalnih in vivo supstrata ovog enzima. Enzim je inhibiran kompetitivno fosfatom (Ki=1,25 mM), molibdatom (Ki=0,055 mM) i pirofosfatom (Ki=6,7 mM) a nekompetitivno fluoridom (Ki= 9,8mM). Joni metala, kao što su Hg2+, Cu2+ i Zn2+ iskazuju inhibitorni efekat na enzim, dok je efekat ne-jonskih detergenata, kao što su Tween 20 i Triton X-100 blago aktivirajuć i. Nema promene u aktivnosti enzima u prisustvu tartarata, citrata, EDTA 1,10-fenantrolina i modifikatora sulfhidrilnih grupa kao što su p-hloromerkuribenzoat i N-etilmaleimid.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract
T1  - Izolovanje i delimična karakterizacija kisele fosfataze ekstrakta polena Artemisia vulgaris
EP  - 572
IS  - 8-9
SP  - 567
VL  - 67
UR  - conv_17
ER  - 
@article{
author = "Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija and Bukilica, Mirjana and Mandić, Ljuba M. and Petrović, Spomenka and Jankov, Ratko",
year = "2002",
abstract = "An acid phosphatase from an extract of mugwort (Artemisia vulgaris) pollen was purified by a factor of 48 by a combination of ion exchange and gel-chromatography. The molecular weights of the enzyme were 76 kDa and 73 kDa, determined by gel filtration on a Sephadex G-100 sf column and by SDS PAGE(under reducing and non-reducing conditions), respectively. In analytical isoelectrofocusing, the enzyme appears as two very close bands pI at about 4.2. The optimum pH for the enzyme is 5.4. The apparent Km for p-nitrophenyl phosphate was estimated to be 0.16mM. The purified enzyme has broad specificity, and hydrolyses p-nitrophenyl phosphate and α-naphthyl phosphate. Pyrophosphate and O-phospho-L-tyrosine were estimated to be the best substrates for this enzyme as potential in vivo substrates. The enzyme is inhibited competitively by phosphate (Ki = 1.25 mM), molybdate (Ki = 0.055 mM) and pyrophosphate (Ki = 6.7 mM) and non-competitively by fluoride (Ki = 9.8 mM). Metal ions such as Hg2+, Cu2+ and Zn2+ express an inhibitory effect on the enzyme, while the enzyme is slightly activated by non-ionic detergents, Tween 20 and Triton X-100. There is no change in the enzyme activity in the presence of tartrate, citrate, EDTA, 1,10-phenanthroline and sulfhydryl-group modifiers such as p-chloromercuribenzoate and N-ethylmaleimide., Kisela fosfataza ekstrakta polena visokog korova (Artemisia vulgaris) je prečišćena 48 puta kombinacijom jonoizmenjivačke i gel-hromatografije. Molekulska težina enzima je 76 kDa i 73 kDa, određena gel-filtracijom na matriksu Sephadex G-100 sf i SDS PAG elektroforezom (pri redukujućim i neredukujućim uslovima), respektivno. Pri izoelektrofokusiranju, enzim se sastoji iz dve vrlo bliske trake pI vrednosti oko 4,2. Optimalno pH za aktivnost enzima je 5,4. PrividnoKmza hidrolizu p-nitrofenil-fosfata je procenjeno da je 0,16 mM. Prečišćeni enzim ima široku specifičnost hidrolizuje p-nitrofenil-fosfat i α-naftil-fosfat. Pirofosfat i O-fosfo-L-tirozin su procenjeni kao najbolji od potencijalnih in vivo supstrata ovog enzima. Enzim je inhibiran kompetitivno fosfatom (Ki=1,25 mM), molibdatom (Ki=0,055 mM) i pirofosfatom (Ki=6,7 mM) a nekompetitivno fluoridom (Ki= 9,8mM). Joni metala, kao što su Hg2+, Cu2+ i Zn2+ iskazuju inhibitorni efekat na enzim, dok je efekat ne-jonskih detergenata, kao što su Tween 20 i Triton X-100 blago aktivirajuć i. Nema promene u aktivnosti enzima u prisustvu tartarata, citrata, EDTA 1,10-fenantrolina i modifikatora sulfhidrilnih grupa kao što su p-hloromerkuribenzoat i N-etilmaleimid.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract, Izolovanje i delimična karakterizacija kisele fosfataze ekstrakta polena Artemisia vulgaris",
pages = "572-567",
number = "8-9",
volume = "67",
url = "conv_17"
}
Ćirković-Veličković, T., Gavrović-Jankulović, M., Bukilica, M., Mandić, L. M., Petrović, S.,& Jankov, R.. (2002). Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 67(8-9), 567-572.
conv_17
Ćirković-Veličković T, Gavrović-Jankulović M, Bukilica M, Mandić LM, Petrović S, Jankov R. Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract. in Journal of the Serbian Chemical Society. 2002;67(8-9):567-572.
conv_17 .
Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, Bukilica, Mirjana, Mandić, Ljuba M., Petrović, Spomenka, Jankov, Ratko, "Isolation and partial characterization of an acid phosphatase from Artemisia vulgaris pollen extract" in Journal of the Serbian Chemical Society, 67, no. 8-9 (2002):567-572,
conv_17 .