TY  - JOUR
AU  - Knežević, Sanja
AU  - Kosanović, Dejana
AU  - Dragačević, Luka
AU  - Živković, Irena
AU  - Ilić, Vesna
AU  - Hajduković, Ljiljana
AU  - Savić, Olivera
AU  - Minić, Rajna
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/651
AB  - S. pneumoniae is an important human pathogen which has a polysaccharide capsule with virulent properties. This work aims to estimate the titres of S. pneumoniae specific IgG and IgA isotypes, with respect to age and sex. An in-house whole bacterial cell ELISA was used for the determination of relative levels and endpoint titres of IgG subclasses and IgA1 subclass specific for S. pneumoniae serogroup 1, and to quantify specific IgG1 and IgG2 levels. Significantly lower anti-pneumococcus IgG1 titres were found in older individuals, which was more pronounced in men. Lower IgG2 titres were detected in men over 50 years of age, in comparison to women under 50 years of age. The levels of IgG3 and IgG4 did not differ between different sex and age groups. Lower IgA1 levels were detected in male individuals in both age groups in comparison to females under 50 years of age. The levels of IgG1 showed a moderate correlation with IgG4 in younger individuals of both sexes (r = 0.61 in men and 0.63 in women) which was not noted in the older age group. We highlight the deficiency in humoral immunity in older people, especially male and suggest immunization of this population with pneumococcal vaccines.
PB  - Elsevier
T2  - Comparative Immunology, Microbiology and Infectious Diseases
T1  - Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1
SP  - 101834
VL  - 87
DO  - 10.1016/j.cimid.2022.101834
ER  - 

@article{
author = "Knežević, Sanja and Kosanović, Dejana and Dragačević, Luka and Živković, Irena and Ilić, Vesna and Hajduković, Ljiljana and Savić, Olivera and Minić, Rajna",
year = "2022",
abstract = "S. pneumoniae is an important human pathogen which has a polysaccharide capsule with virulent properties. This work aims to estimate the titres of S. pneumoniae specific IgG and IgA isotypes, with respect to age and sex. An in-house whole bacterial cell ELISA was used for the determination of relative levels and endpoint titres of IgG subclasses and IgA1 subclass specific for S. pneumoniae serogroup 1, and to quantify specific IgG1 and IgG2 levels. Significantly lower anti-pneumococcus IgG1 titres were found in older individuals, which was more pronounced in men. Lower IgG2 titres were detected in men over 50 years of age, in comparison to women under 50 years of age. The levels of IgG3 and IgG4 did not differ between different sex and age groups. Lower IgA1 levels were detected in male individuals in both age groups in comparison to females under 50 years of age. The levels of IgG1 showed a moderate correlation with IgG4 in younger individuals of both sexes (r = 0.61 in men and 0.63 in women) which was not noted in the older age group. We highlight the deficiency in humoral immunity in older people, especially male and suggest immunization of this population with pneumococcal vaccines.",
publisher = "Elsevier",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
title = "Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1",
pages = "101834",
volume = "87",
doi = "10.1016/j.cimid.2022.101834"
}

Knežević, S., Kosanović, D., Dragačević, L., Živković, I., Ilić, V., Hajduković, L., Savić, O.,& Minić, R.. (2022). Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1. in Comparative Immunology, Microbiology and Infectious Diseases
Elsevier., 87, 101834.
https://doi.org/10.1016/j.cimid.2022.101834

Knežević S, Kosanović D, Dragačević L, Živković I, Ilić V, Hajduković L, Savić O, Minić R. Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1. in Comparative Immunology, Microbiology and Infectious Diseases. 2022;87:101834.
doi:10.1016/j.cimid.2022.101834 .

Knežević, Sanja, Kosanović, Dejana, Dragačević, Luka, Živković, Irena, Ilić, Vesna, Hajduković, Ljiljana, Savić, Olivera, Minić, Rajna, "Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1" in Comparative Immunology, Microbiology and Infectious Diseases, 87 (2022):101834,
https://doi.org/10.1016/j.cimid.2022.101834 . .

TY  - JOUR
AU  - Knežević, Sanja
AU  - Kosanović, Dejana
AU  - Dragačević, Luka
AU  - Živković, Irena
AU  - Ilić, Vesna
AU  - Hajduković, Ljiljana
AU  - Savić, Olivera
AU  - Minić, Rajna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1255
UR  - http://intor.torlakinstitut.com/handle/123456789/650
AB  - S. pneumoniae is an important human pathogen which has a polysaccharide capsule with virulent properties. This work aims to estimate the titres of S. pneumoniae specific IgG and IgA isotypes, with respect to age and sex. An in-house whole bacterial cell ELISA was used for the determination of relative levels and endpoint titres of IgG subclasses and IgA1 subclass specific for S. pneumoniae serogroup 1, and to quantify specific IgG1 and IgG2 levels. Significantly lower anti-pneumococcus IgG1 titres were found in older individuals, which was more pronounced in men. Lower IgG2 titres were detected in men over 50 years of age, in comparison to women under 50 years of age. The levels of IgG3 and IgG4 did not differ between different sex and age groups. Lower IgA1 levels were detected in male individuals in both age groups in comparison to females under 50 years of age. The levels of IgG1 showed a moderate correlation with IgG4 in younger individuals of both sexes (r = 0.61 in men and 0.63 in women) which was not noted in the older age group. We highlight the deficiency in humoral immunity in older people, especially male and suggest immunization of this population with pneumococcal vaccines.
PB  - Elsevier
T2  - Comparative Immunology, Microbiology and Infectious Diseases
T1  - Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1
SP  - 101834
VL  - 87
DO  - 10.1016/j.cimid.2022.101834
ER  - 

@article{
author = "Knežević, Sanja and Kosanović, Dejana and Dragačević, Luka and Živković, Irena and Ilić, Vesna and Hajduković, Ljiljana and Savić, Olivera and Minić, Rajna",
year = "2022",
abstract = "S. pneumoniae is an important human pathogen which has a polysaccharide capsule with virulent properties. This work aims to estimate the titres of S. pneumoniae specific IgG and IgA isotypes, with respect to age and sex. An in-house whole bacterial cell ELISA was used for the determination of relative levels and endpoint titres of IgG subclasses and IgA1 subclass specific for S. pneumoniae serogroup 1, and to quantify specific IgG1 and IgG2 levels. Significantly lower anti-pneumococcus IgG1 titres were found in older individuals, which was more pronounced in men. Lower IgG2 titres were detected in men over 50 years of age, in comparison to women under 50 years of age. The levels of IgG3 and IgG4 did not differ between different sex and age groups. Lower IgA1 levels were detected in male individuals in both age groups in comparison to females under 50 years of age. The levels of IgG1 showed a moderate correlation with IgG4 in younger individuals of both sexes (r = 0.61 in men and 0.63 in women) which was not noted in the older age group. We highlight the deficiency in humoral immunity in older people, especially male and suggest immunization of this population with pneumococcal vaccines.",
publisher = "Elsevier",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
title = "Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1",
pages = "101834",
volume = "87",
doi = "10.1016/j.cimid.2022.101834"
}

Knežević, S., Kosanović, D., Dragačević, L., Živković, I., Ilić, V., Hajduković, L., Savić, O.,& Minić, R.. (2022). Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1. in Comparative Immunology, Microbiology and Infectious Diseases
Elsevier., 87, 101834.
https://doi.org/10.1016/j.cimid.2022.101834

Knežević S, Kosanović D, Dragačević L, Živković I, Ilić V, Hajduković L, Savić O, Minić R. Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1. in Comparative Immunology, Microbiology and Infectious Diseases. 2022;87:101834.
doi:10.1016/j.cimid.2022.101834 .

Knežević, Sanja, Kosanović, Dejana, Dragačević, Luka, Živković, Irena, Ilić, Vesna, Hajduković, Ljiljana, Savić, Olivera, Minić, Rajna, "Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1" in Comparative Immunology, Microbiology and Infectious Diseases, 87 (2022):101834,
https://doi.org/10.1016/j.cimid.2022.101834 . .

TY  - JOUR
AU  - Micić, Milutin
AU  - Antonijević, Đorđe
AU  - Milutinović-Smiljanić, Sanja
AU  - Trišić, Dijana
AU  - Čolović, Božana M.
AU  - Kosanović, Dejana
AU  - Prokić, Bogomir Bolka
AU  - Vasić, Jugoslav
AU  - Živković, Slavoljub
AU  - Milašin, Jelena
AU  - Danilović, Vesna
AU  - Đurić, Marija P.
AU  - Jokanović, Vukoman R.
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/660
AB  - Article Corrigendum to: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit’s ulna reconstruction was published on June 28, 2022 in the journal Biomedical Engineering / Biomedizinische Technik
T2  - Biomedical Engineering / Biomedizinische Technik
T1  - Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218)
DO  - 10.1515/bmt-2022-0188
ER  - 

@article{
author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trišić, Dijana and Čolović, Božana M. and Kosanović, Dejana and Prokić, Bogomir Bolka and Vasić, Jugoslav and Živković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija P. and Jokanović, Vukoman R.",
year = "2022",
abstract = "Article Corrigendum to: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit’s ulna reconstruction was published on June 28, 2022 in the journal Biomedical Engineering / Biomedizinische Technik",
journal = "Biomedical Engineering / Biomedizinische Technik",
title = "Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218)",
doi = "10.1515/bmt-2022-0188"
}

Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trišić, D., Čolović, B. M., Kosanović, D., Prokić, B. B., Vasić, J., Živković, S., Milašin, J., Danilović, V., Đurić, M. P.,& Jokanović, V. R.. (2022). Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218). in Biomedical Engineering / Biomedizinische Technik.
https://doi.org/10.1515/bmt-2022-0188

Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trišić D, Čolović BM, Kosanović D, Prokić BB, Vasić J, Živković S, Milašin J, Danilović V, Đurić MP, Jokanović VR. Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218). in Biomedical Engineering / Biomedizinische Technik. 2022;.
doi:10.1515/bmt-2022-0188 .

Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trišić, Dijana, Čolović, Božana M., Kosanović, Dejana, Prokić, Bogomir Bolka, Vasić, Jugoslav, Živković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija P., Jokanović, Vukoman R., "Corrigendum: Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: Physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction (Biomedical Engineering / Biomedizinische Technik 65:4 (491-505) DOI: 10.1515/bmt-2019-0218)" in Biomedical Engineering / Biomedizinische Technik (2022),
https://doi.org/10.1515/bmt-2022-0188 . .

TY  - DATA
AU  - Kosanović, Dejana
AU  - Dyas, Maria
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/638
AB  - Table S1. Bacterial species used in this study with amplicon deposition number obtained from NCBI GeneBank; Figure S1. 16S rRNA gene analysis; Figure S2. Specific protein analysis- gyrB gene analysis; Figure S3a. Zone of inhibition test on NA plates104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 30°C; Figure S3b. Zone of inhibition test on YMEA and NA plates 104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 25°C; Figure S4. The effect of 25%v/v bacterial supernatants on T. aggressivum growth was assessed. B. subtilis R8.3 supernatant was found to inhibit growth of T. aggressivum by 37% and that was the maximum effect compared to other bacterial supernatants; Figure S5. PCA analysis. □ - Control group and ∆ - T. aggresivum + B. velezensis SN group; Figure S6. Protein abundance similarities of two sample group (T. aggressivum control and T. aggressivum treated with B. velezensis SN) based on hierarchical clustering; Figure S7; Figure S8. PCA analysis. □ - Control group and ∆ - A. bisporus + B. velezensis SN group.
PB  - Springer
T2  - European Journal of Plant Pathology
T1  - Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.
IS  - 2
VL  - 160
VL  - 397
UR  - https://hdl.handle.net/21.15107/rcub_intor_638
ER  - 

@misc{
author = "Kosanović, Dejana and Dyas, Maria and Grogan, Helen and Kavanagh, Kevin",
year = "2021",
abstract = "Table S1. Bacterial species used in this study with amplicon deposition number obtained from NCBI GeneBank; Figure S1. 16S rRNA gene analysis; Figure S2. Specific protein analysis- gyrB gene analysis; Figure S3a. Zone of inhibition test on NA plates104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 30°C; Figure S3b. Zone of inhibition test on YMEA and NA plates 104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 25°C; Figure S4. The effect of 25%v/v bacterial supernatants on T. aggressivum growth was assessed. B. subtilis R8.3 supernatant was found to inhibit growth of T. aggressivum by 37% and that was the maximum effect compared to other bacterial supernatants; Figure S5. PCA analysis. □ - Control group and ∆ - T. aggresivum + B. velezensis SN group; Figure S6. Protein abundance similarities of two sample group (T. aggressivum control and T. aggressivum treated with B. velezensis SN) based on hierarchical clustering; Figure S7; Figure S8. PCA analysis. □ - Control group and ∆ - A. bisporus + B. velezensis SN group.",
publisher = "Springer",
journal = "European Journal of Plant Pathology",
title = "Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.",
number = "2",
volume = "160, 397",
url = "https://hdl.handle.net/21.15107/rcub_intor_638"
}

Kosanović, D., Dyas, M., Grogan, H.,& Kavanagh, K.. (2021). Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.. in European Journal of Plant Pathology
Springer., 160(2).
https://hdl.handle.net/21.15107/rcub_intor_638

Kosanović D, Dyas M, Grogan H, Kavanagh K. Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.. in European Journal of Plant Pathology. 2021;160(2).
https://hdl.handle.net/21.15107/rcub_intor_638 .

Kosanović, Dejana, Dyas, Maria, Grogan, Helen, Kavanagh, Kevin, "Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5." in European Journal of Plant Pathology, 160, no. 2 (2021),
https://hdl.handle.net/21.15107/rcub_intor_638 .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kosanović, Dejana
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Ljubobratović, Uroš
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/569
AB  - This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.
PB  - Elsevier, Amsterdam
T2  - Animal Feed Science and Technology
T1  - Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner
VL  - 259
DO  - 10.1016/j.anifeedsci.2019.114356
ER  - 

@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Kosanović, Dejana and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Jeney, Galina and Ljubobratović, Uroš",
year = "2020",
abstract = "This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.",
publisher = "Elsevier, Amsterdam",
journal = "Animal Feed Science and Technology",
title = "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner",
volume = "259",
doi = "10.1016/j.anifeedsci.2019.114356"
}

Lukić, J., Stanisavljević, N., Vukotić, G., Kosanović, D., Terzić-Vidojević, A., Begović, J., Golić, N., Jeney, G.,& Ljubobratović, U.. (2020). Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology
Elsevier, Amsterdam., 259.
https://doi.org/10.1016/j.anifeedsci.2019.114356

Lukić J, Stanisavljević N, Vukotić G, Kosanović D, Terzić-Vidojević A, Begović J, Golić N, Jeney G, Ljubobratović U. Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology. 2020;259.
doi:10.1016/j.anifeedsci.2019.114356 .

Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Kosanović, Dejana, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Jeney, Galina, Ljubobratović, Uroš, "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner" in Animal Feed Science and Technology, 259 (2020),
https://doi.org/10.1016/j.anifeedsci.2019.114356 . .

TY  - JOUR
AU  - Ljubobratović, Uroš
AU  - Kosanović, Dejana
AU  - Demeny, Ferenc Zoltan
AU  - Krajcsovics, Adrienn
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Lukić, Jovanka
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/562
AB  - Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae
VL  - 516
DO  - 10.1016/j.aquaculture.2019.734608
ER  - 

@article{
author = "Ljubobratović, Uroš and Kosanović, Dejana and Demeny, Ferenc Zoltan and Krajcsovics, Adrienn and Vukotić, Goran and Stanisavljević, Nemanja and Golić, Nataša and Jeney, Galina and Lukić, Jovanka",
year = "2020",
abstract = "Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae",
volume = "516",
doi = "10.1016/j.aquaculture.2019.734608"
}

Ljubobratović, U., Kosanović, D., Demeny, F. Z., Krajcsovics, A., Vukotić, G., Stanisavljević, N., Golić, N., Jeney, G.,& Lukić, J.. (2020). The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture
Elsevier, Amsterdam., 516.
https://doi.org/10.1016/j.aquaculture.2019.734608

Ljubobratović U, Kosanović D, Demeny FZ, Krajcsovics A, Vukotić G, Stanisavljević N, Golić N, Jeney G, Lukić J. The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture. 2020;516.
doi:10.1016/j.aquaculture.2019.734608 .

Ljubobratović, Uroš, Kosanović, Dejana, Demeny, Ferenc Zoltan, Krajcsovics, Adrienn, Vukotić, Goran, Stanisavljević, Nemanja, Golić, Nataša, Jeney, Galina, Lukić, Jovanka, "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae" in Aquaculture, 516 (2020),
https://doi.org/10.1016/j.aquaculture.2019.734608 . .

TY  - DATA
AU  - Micić, Milutin
AU  - Antonijević, Đorđe
AU  - Milutinović-Smiljanić, Sanja
AU  - Trisić, Dijana
AU  - Colović, Božana
AU  - Kosanović, Dejana
AU  - Prokić, Bogomir
AU  - Vasić, Jugoslav
AU  - Zivković, Slavoljub
AU  - Milašin, Jelena
AU  - Danilović, Vesna
AU  - Đurić, Marija
AU  - Jokanović, Vukoman
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/661
AB  - Figure 1S. Change of the Ca concentration in the solution containing investigated nHAP granules with time.  Figure 2S. Change of the Ca concentration with time in biological apatite (BioOss).  Figure 3S. The correlation of the ALBO-OS compressive strenght and the time of the material’s soaking in Hank’s solution. Table 1S. Reference point indentation outcomes of the ALBOOS. MH – microhardness, ID 1st - 1st Cycle Indentation Distance; US 1st - 1st Cycle Unloading Slope; CID 1st -1st Cycle Creep Indentation Distance; TID - Total Indentation Distance; IDI - Indentation Distance Increase; Avg CID - Avg Creep Indentation Distance; Avg US - Average Unloading Slope; Avg US - Average Unloading Slope; Avg LS - Average Loading Slope; Avg ED - Average Energy Dissipated.  Table 2S. Ph of nHAP after soaking in simulated body fluid. Figure 4S. Microacrhitectural and structural characteristics of nHAP in vitro and in contact with bone tissue in vivo. Adequate microstructure and surface nanotopography provide good environment for cells infiltration in vivo. Note the presence of porous paterns within material structure in vivo as well as the lamellar structure of ALBO-OS.  Figure 5S. Schematic representation of the extruder design in custom made laboratory 3D printer: from technical reasons, 3D printer was modified to include two extruders: 1 – syringe extruder, 1.1 – glass syringe, 1.2 – external frame of the extruder 1, 1.3 – electrical heater coil, 1.4 –metal piston of the syringe, 1.5 – syringe nozzle (0,8mm in diameter), 1.6 – gears of the piston drive, 1.7 – piston drive motor with reduction, 1.8 – HAP PLA mixture, 2 – PLA extruder, 2.1 – heater block of the PLA extruder, 2.2 – extruder cooler, 2.3 – PLA extruder motor drive with reduction, 2.4 – PLA filament 1.75mm in diameter, 2.5 – PLA extruder nozzle 0,4mm in diameter.  Figure 6S. Paste extruder attached to the head of the printer, with removed electrical heater coil, so the syringe with the metal piston can be visible.  A) The engineered construct used for the experiments and B) its structure after magnification (X200). C-E) Wettability of the construct. Administration of the reference liquid droplet on the construct surface. F) Histogram showing the time required for reference liquid to completely wet the construct surface. Note that the droplet is absorbed within the part of the second.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biomedical Engineering-Biomedizinische Technik
T1  - Supplementary information for the article: Micić, M.; Antonijević, Đ.; Milutinović-Smiljanić, S.; Trisić, D.;  Colović, B.; Kosanović, D.; Prokić, B.; Vasić, J.; Zivković, S.; Milašin, J.; Danilović, V.; Đurić, M.; Jokanović, V. Developing  a Novel Resorptive Hydroxyapatite-Based Bone Substitute for over-Critical Size Defect Reconstruction: Physicochemical and  Biological Characterization and Proof of Concept in Segmental Rabbit’s Ulna Reconstruction. Biomedical  Engineering-Biomedizinische Technik 2020, 65 (4), 491–505. https://doi.org/10.1515/bmt-2019-0218.
UR  - https://hdl.handle.net/21.15107/rcub_intor_661
ER  - 

@misc{
author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trisić, Dijana and Colović, Božana and Kosanović, Dejana and Prokić, Bogomir and Vasić, Jugoslav and Zivković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija and Jokanović, Vukoman",
year = "2020",
abstract = "Figure 1S. Change of the Ca concentration in the solution containing investigated nHAP granules with time.  Figure 2S. Change of the Ca concentration with time in biological apatite (BioOss).  Figure 3S. The correlation of the ALBO-OS compressive strenght and the time of the material’s soaking in Hank’s solution. Table 1S. Reference point indentation outcomes of the ALBOOS. MH – microhardness, ID 1st - 1st Cycle Indentation Distance; US 1st - 1st Cycle Unloading Slope; CID 1st -1st Cycle Creep Indentation Distance; TID - Total Indentation Distance; IDI - Indentation Distance Increase; Avg CID - Avg Creep Indentation Distance; Avg US - Average Unloading Slope; Avg US - Average Unloading Slope; Avg LS - Average Loading Slope; Avg ED - Average Energy Dissipated.  Table 2S. Ph of nHAP after soaking in simulated body fluid. Figure 4S. Microacrhitectural and structural characteristics of nHAP in vitro and in contact with bone tissue in vivo. Adequate microstructure and surface nanotopography provide good environment for cells infiltration in vivo. Note the presence of porous paterns within material structure in vivo as well as the lamellar structure of ALBO-OS.  Figure 5S. Schematic representation of the extruder design in custom made laboratory 3D printer: from technical reasons, 3D printer was modified to include two extruders: 1 – syringe extruder, 1.1 – glass syringe, 1.2 – external frame of the extruder 1, 1.3 – electrical heater coil, 1.4 –metal piston of the syringe, 1.5 – syringe nozzle (0,8mm in diameter), 1.6 – gears of the piston drive, 1.7 – piston drive motor with reduction, 1.8 – HAP PLA mixture, 2 – PLA extruder, 2.1 – heater block of the PLA extruder, 2.2 – extruder cooler, 2.3 – PLA extruder motor drive with reduction, 2.4 – PLA filament 1.75mm in diameter, 2.5 – PLA extruder nozzle 0,4mm in diameter.  Figure 6S. Paste extruder attached to the head of the printer, with removed electrical heater coil, so the syringe with the metal piston can be visible.  A) The engineered construct used for the experiments and B) its structure after magnification (X200). C-E) Wettability of the construct. Administration of the reference liquid droplet on the construct surface. F) Histogram showing the time required for reference liquid to completely wet the construct surface. Note that the droplet is absorbed within the part of the second.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biomedical Engineering-Biomedizinische Technik",
title = "Supplementary information for the article: Micić, M.; Antonijević, Đ.; Milutinović-Smiljanić, S.; Trisić, D.;  Colović, B.; Kosanović, D.; Prokić, B.; Vasić, J.; Zivković, S.; Milašin, J.; Danilović, V.; Đurić, M.; Jokanović, V. Developing  a Novel Resorptive Hydroxyapatite-Based Bone Substitute for over-Critical Size Defect Reconstruction: Physicochemical and  Biological Characterization and Proof of Concept in Segmental Rabbit’s Ulna Reconstruction. Biomedical  Engineering-Biomedizinische Technik 2020, 65 (4), 491–505. https://doi.org/10.1515/bmt-2019-0218.",
url = "https://hdl.handle.net/21.15107/rcub_intor_661"
}

Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trisić, D., Colović, B., Kosanović, D., Prokić, B., Vasić, J., Zivković, S., Milašin, J., Danilović, V., Đurić, M.,& Jokanović, V.. (2020). Supplementary information for the article: Micić, M.; Antonijević, Đ.; Milutinović-Smiljanić, S.; Trisić, D.;  Colović, B.; Kosanović, D.; Prokić, B.; Vasić, J.; Zivković, S.; Milašin, J.; Danilović, V.; Đurić, M.; Jokanović, V. Developing  a Novel Resorptive Hydroxyapatite-Based Bone Substitute for over-Critical Size Defect Reconstruction: Physicochemical and  Biological Characterization and Proof of Concept in Segmental Rabbit’s Ulna Reconstruction. Biomedical  Engineering-Biomedizinische Technik 2020, 65 (4), 491–505. https://doi.org/10.1515/bmt-2019-0218.. in Biomedical Engineering-Biomedizinische Technik
Walter De Gruyter Gmbh, Berlin..
https://hdl.handle.net/21.15107/rcub_intor_661

Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trisić D, Colović B, Kosanović D, Prokić B, Vasić J, Zivković S, Milašin J, Danilović V, Đurić M, Jokanović V. Supplementary information for the article: Micić, M.; Antonijević, Đ.; Milutinović-Smiljanić, S.; Trisić, D.;  Colović, B.; Kosanović, D.; Prokić, B.; Vasić, J.; Zivković, S.; Milašin, J.; Danilović, V.; Đurić, M.; Jokanović, V. Developing  a Novel Resorptive Hydroxyapatite-Based Bone Substitute for over-Critical Size Defect Reconstruction: Physicochemical and  Biological Characterization and Proof of Concept in Segmental Rabbit’s Ulna Reconstruction. Biomedical  Engineering-Biomedizinische Technik 2020, 65 (4), 491–505. https://doi.org/10.1515/bmt-2019-0218.. in Biomedical Engineering-Biomedizinische Technik. 2020;.
https://hdl.handle.net/21.15107/rcub_intor_661 .

Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trisić, Dijana, Colović, Božana, Kosanović, Dejana, Prokić, Bogomir, Vasić, Jugoslav, Zivković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija, Jokanović, Vukoman, "Supplementary information for the article: Micić, M.; Antonijević, Đ.; Milutinović-Smiljanić, S.; Trisić, D.;  Colović, B.; Kosanović, D.; Prokić, B.; Vasić, J.; Zivković, S.; Milašin, J.; Danilović, V.; Đurić, M.; Jokanović, V. Developing  a Novel Resorptive Hydroxyapatite-Based Bone Substitute for over-Critical Size Defect Reconstruction: Physicochemical and  Biological Characterization and Proof of Concept in Segmental Rabbit’s Ulna Reconstruction. Biomedical  Engineering-Biomedizinische Technik 2020, 65 (4), 491–505. https://doi.org/10.1515/bmt-2019-0218." in Biomedical Engineering-Biomedizinische Technik (2020),
https://hdl.handle.net/21.15107/rcub_intor_661 .

TY  - JOUR
AU  - Micić, Milutin
AU  - Antonijević, Đorđe
AU  - Milutinović-Smiljanić, Sanja
AU  - Trisić, Dijana
AU  - Colović, Božana
AU  - Kosanović, Dejana
AU  - Prokić, Bogomir
AU  - Vasić, Jugoslav
AU  - Zivković, Slavoljub
AU  - Milašin, Jelena
AU  - Danilović, Vesna
AU  - Đurić, Marija
AU  - Jokanović, Vukoman
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/542
AB  - The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 mu m. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators - bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biomedical Engineering-Biomedizinische Technik
T1  - Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction
EP  - 505
IS  - 4
SP  - 491
VL  - 65
DO  - 10.1515/bmt-2019-0218
ER  - 

@article{
author = "Micić, Milutin and Antonijević, Đorđe and Milutinović-Smiljanić, Sanja and Trisić, Dijana and Colović, Božana and Kosanović, Dejana and Prokić, Bogomir and Vasić, Jugoslav and Zivković, Slavoljub and Milašin, Jelena and Danilović, Vesna and Đurić, Marija and Jokanović, Vukoman",
year = "2020",
abstract = "The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 mu m. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators - bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biomedical Engineering-Biomedizinische Technik",
title = "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction",
pages = "505-491",
number = "4",
volume = "65",
doi = "10.1515/bmt-2019-0218"
}

Micić, M., Antonijević, Đ., Milutinović-Smiljanić, S., Trisić, D., Colović, B., Kosanović, D., Prokić, B., Vasić, J., Zivković, S., Milašin, J., Danilović, V., Đurić, M.,& Jokanović, V.. (2020). Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedical Engineering-Biomedizinische Technik
Walter De Gruyter Gmbh, Berlin., 65(4), 491-505.
https://doi.org/10.1515/bmt-2019-0218

Micić M, Antonijević Đ, Milutinović-Smiljanić S, Trisić D, Colović B, Kosanović D, Prokić B, Vasić J, Zivković S, Milašin J, Danilović V, Đurić M, Jokanović V. Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction. in Biomedical Engineering-Biomedizinische Technik. 2020;65(4):491-505.
doi:10.1515/bmt-2019-0218 .

Micić, Milutin, Antonijević, Đorđe, Milutinović-Smiljanić, Sanja, Trisić, Dijana, Colović, Božana, Kosanović, Dejana, Prokić, Bogomir, Vasić, Jugoslav, Zivković, Slavoljub, Milašin, Jelena, Danilović, Vesna, Đurić, Marija, Jokanović, Vukoman, "Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction" in Biomedical Engineering-Biomedizinische Technik, 65, no. 4 (2020):491-505,
https://doi.org/10.1515/bmt-2019-0218 . .

TY  - JOUR
AU  - Kosanović, Dejana
AU  - Sheehan, Gerard
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/559
AB  - Green mould disease is caused by Trichoderma aggressivum which colonizes mushroom compost and reduces yield. Two Pseudomonas species are associated with mushroom compost: Pseudomonas putida, which stimulates mushroom pinning, and Pseudomonas tolaasii which has a negative effect on crop production. The aim of this work was to characterize T. aggressivum – Pseudomonas interactions as these may be important factors in the development of green mould disease. P. tolaasii supernatant inhibited growth by 57% but P. putida stimulated growth of T.aggressivum by 44%. Tolaasin production was identified in P. tolaasii cultures with a peak at 96 h. Fluorescent microscopy examination of T. aggressivum hyphae revealed that exposure to P. tolaasii supernatant decreased mycelial formation while increasing the abundance of conidia. Label free proteomic analysis of changes in the abundance of T. aggressivum proteins indicated that exposure to P. tolaasii supernatant lead to an oxidative stress response and catabolic enzyme activation (mitochondrial import inner membrane translocase complex (5.7-fold), oxidoreductase (5.2-fold), glucoamylase (5.1-fold)). Exposure of T. aggressivum to P. putida supernatant lead to an increase in the abundance of proteins associated with growth and development (structural constituents of ribosome (20-fold), H/ACA ribonucleoprotein complex subunit (18-fold), DNA binding and nucleosome assembly protein (5.3-fold), and prefoldin (5-fold)). These results indicate that exposure to P. putida can stimulate the growth of T. aggressivum and this interaction may be an important factor in increasing green mould disease in mushroom crops and so reducing yield.
T2  - European Journal of Plant Pathology
T1  - Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum
EP  - 121
IS  - 1
SP  - 111
VL  - 156
DO  - 10.1007/s10658-019-01867-z
UR  - https://hdl.handle.net/21.15107/rcub_intor_559
ER  - 

@article{
author = "Kosanović, Dejana and Sheehan, Gerard and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Green mould disease is caused by Trichoderma aggressivum which colonizes mushroom compost and reduces yield. Two Pseudomonas species are associated with mushroom compost: Pseudomonas putida, which stimulates mushroom pinning, and Pseudomonas tolaasii which has a negative effect on crop production. The aim of this work was to characterize T. aggressivum – Pseudomonas interactions as these may be important factors in the development of green mould disease. P. tolaasii supernatant inhibited growth by 57% but P. putida stimulated growth of T.aggressivum by 44%. Tolaasin production was identified in P. tolaasii cultures with a peak at 96 h. Fluorescent microscopy examination of T. aggressivum hyphae revealed that exposure to P. tolaasii supernatant decreased mycelial formation while increasing the abundance of conidia. Label free proteomic analysis of changes in the abundance of T. aggressivum proteins indicated that exposure to P. tolaasii supernatant lead to an oxidative stress response and catabolic enzyme activation (mitochondrial import inner membrane translocase complex (5.7-fold), oxidoreductase (5.2-fold), glucoamylase (5.1-fold)). Exposure of T. aggressivum to P. putida supernatant lead to an increase in the abundance of proteins associated with growth and development (structural constituents of ribosome (20-fold), H/ACA ribonucleoprotein complex subunit (18-fold), DNA binding and nucleosome assembly protein (5.3-fold), and prefoldin (5-fold)). These results indicate that exposure to P. putida can stimulate the growth of T. aggressivum and this interaction may be an important factor in increasing green mould disease in mushroom crops and so reducing yield.",
journal = "European Journal of Plant Pathology",
title = "Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum",
pages = "121-111",
number = "1",
volume = "156",
doi = "10.1007/s10658-019-01867-z",
url = "https://hdl.handle.net/21.15107/rcub_intor_559"
}

Kosanović, D., Sheehan, G., Grogan, H.,& Kavanagh, K.. (2020). Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum. in European Journal of Plant Pathology, 156(1), 111-121.
https://doi.org/10.1007/s10658-019-01867-z
https://hdl.handle.net/21.15107/rcub_intor_559

Kosanović D, Sheehan G, Grogan H, Kavanagh K. Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum. in European Journal of Plant Pathology. 2020;156(1):111-121.
doi:10.1007/s10658-019-01867-z
https://hdl.handle.net/21.15107/rcub_intor_559 .

Kosanović, Dejana, Sheehan, Gerard, Grogan, Helen, Kavanagh, Kevin, "Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum" in European Journal of Plant Pathology, 156, no. 1 (2020):111-121,
https://doi.org/10.1007/s10658-019-01867-z .,
https://hdl.handle.net/21.15107/rcub_intor_559 .

TY  - DATA
AU  - Kosanović, Dejana
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/640
AB  - Figure S1. Produce Phase III substrate which was heavily colonised by T. aggressivum and used as inoculum for subsequent experiment; Figure S2. Most probable number (MPN) analysis results, a) -2 dilution level, b) -5 dilution level; Figure S3. PCR verification of T. aggressivum f. europaeum. Wells 2,3,4 are negativeve control (culture FM5 T. harzianum). Wells 5,6,7 are T. aggressivum FM10. Wells 6,7 are very faint in the image unfortunately, but they were on the gel. We can still slightly see them. Sample CBS 100526 is in wells 8,9,10 and they are all clearly positive. Well 8 is Trichoderma universal (ITS1/4 primers), well 9 is for both Th2/Th4 biotypes (TH1 INT/ITS4 primers). Th2 biotype is T. aggressivum f. europaeum, Th4 biotype is T. aggressivum f. aggressivum, and in well 10 is Th2 biotype specific (i.e. T. aggressivum f. europium, 18S/TH1 INT REV primers); Figure S4. Principal component analysis (PCA statistical procedure). ▫ - T. aggressivum group and ▫ - Control group; Figure S5 a. Increase/Decrease in biological processes in A. bisporus after 4 days treatment with T. aggressivum 48h supernatant; Figure S5 b. Increase/Decrease in molecular function in A. bisporus after 4 days treatment with T. aggressivum 48h supernatant; Figure S5 c. Increased/decreased cellular components in A. bisporus after 4 day treatment with T. aggressivum 48h supernatant; Figure S5 d. Increase/decrease in enzyme activity of A. bisporus after 4 day of treatment with 48h T. aggressivum supernatant; Figure S6. Green mold was observed on the mushroom casing of inoculated plots (c:10-3 and b:10-4), but not on the control plots (a). Starting from day 14th dense white mycelia was observed, after few days the color changed into green after extensive sporulation (b, c); Figure S7. Symptoms of green mould (deformities of sporocarp and brown spots) on fruiting body on day 17th after casing the compost; Table S1. Colorimetry assay on mushroom pilei in infected or controlled plots; Figure S8. Proteomic responses of A. bisporus following 25-day incubation with 10-4 inoculum of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 1.5. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S9. Proteomic responses of A. bisporus following 25-day incubation with -3 inoculum of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 1.5. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S10 a. Increased molecular function 25 days after A. bisporus inoculation with T. aggressivum; Figure S10 b. Increased cellular component day 24 after A. bisporus inoculation with T. aggressivum; Figure S10 c. Increased enzymes day 25 after inoculation of A. bisporus with T. aggressivum; Figure S11a. Proteomic responses of A. bisporus following 2 day incubation with 25% v/v 48h supernatant of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 2. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S11b. Proteomic responses of A. bisporus following 8 day incubation with 25% v/v 48h supernatant of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 2. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed
T2  - Fungal Biology
T1  - Supplementary information for the article: Kosanović, D.; Grogan, H.; Kavanagh, K. Exposure of Agaricus Bisporus to Trichoderma Aggressivum f. Europaeum Leads to Growth Inhibition and Induction of an Oxidative Stress Response. Fungal Biology 2020, 124 (9), 814–820. https://doi.org/10.1016/j.funbio.2020.07.003.
EP  - 820
IS  - 9
SP  - 814
VL  - 124
UR  - https://hdl.handle.net/21.15107/rcub_intor_640
ER  - 

@misc{
author = "Kosanović, Dejana and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Figure S1. Produce Phase III substrate which was heavily colonised by T. aggressivum and used as inoculum for subsequent experiment; Figure S2. Most probable number (MPN) analysis results, a) -2 dilution level, b) -5 dilution level; Figure S3. PCR verification of T. aggressivum f. europaeum. Wells 2,3,4 are negativeve control (culture FM5 T. harzianum). Wells 5,6,7 are T. aggressivum FM10. Wells 6,7 are very faint in the image unfortunately, but they were on the gel. We can still slightly see them. Sample CBS 100526 is in wells 8,9,10 and they are all clearly positive. Well 8 is Trichoderma universal (ITS1/4 primers), well 9 is for both Th2/Th4 biotypes (TH1 INT/ITS4 primers). Th2 biotype is T. aggressivum f. europaeum, Th4 biotype is T. aggressivum f. aggressivum, and in well 10 is Th2 biotype specific (i.e. T. aggressivum f. europium, 18S/TH1 INT REV primers); Figure S4. Principal component analysis (PCA statistical procedure). ▫ - T. aggressivum group and ▫ - Control group; Figure S5 a. Increase/Decrease in biological processes in A. bisporus after 4 days treatment with T. aggressivum 48h supernatant; Figure S5 b. Increase/Decrease in molecular function in A. bisporus after 4 days treatment with T. aggressivum 48h supernatant; Figure S5 c. Increased/decreased cellular components in A. bisporus after 4 day treatment with T. aggressivum 48h supernatant; Figure S5 d. Increase/decrease in enzyme activity of A. bisporus after 4 day of treatment with 48h T. aggressivum supernatant; Figure S6. Green mold was observed on the mushroom casing of inoculated plots (c:10-3 and b:10-4), but not on the control plots (a). Starting from day 14th dense white mycelia was observed, after few days the color changed into green after extensive sporulation (b, c); Figure S7. Symptoms of green mould (deformities of sporocarp and brown spots) on fruiting body on day 17th after casing the compost; Table S1. Colorimetry assay on mushroom pilei in infected or controlled plots; Figure S8. Proteomic responses of A. bisporus following 25-day incubation with 10-4 inoculum of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 1.5. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S9. Proteomic responses of A. bisporus following 25-day incubation with -3 inoculum of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 1.5. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S10 a. Increased molecular function 25 days after A. bisporus inoculation with T. aggressivum; Figure S10 b. Increased cellular component day 24 after A. bisporus inoculation with T. aggressivum; Figure S10 c. Increased enzymes day 25 after inoculation of A. bisporus with T. aggressivum; Figure S11a. Proteomic responses of A. bisporus following 2 day incubation with 25% v/v 48h supernatant of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 2. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed data; Figure S11b. Proteomic responses of A. bisporus following 8 day incubation with 25% v/v 48h supernatant of T. aggressivum. Volcano plot represent protein intensity difference (− log2 mean intensity difference) and significance in differences (− log P-value) based on a two-sided t-test. Proteins above the line are considered statistically significant (p value < 0.05) and those to the right and left of the vertical lines indicate relative fold changes > 2. Annotations are given for the most differentially abundant proteins identified. These plots are based upon post imputed",
journal = "Fungal Biology",
title = "Supplementary information for the article: Kosanović, D.; Grogan, H.; Kavanagh, K. Exposure of Agaricus Bisporus to Trichoderma Aggressivum f. Europaeum Leads to Growth Inhibition and Induction of an Oxidative Stress Response. Fungal Biology 2020, 124 (9), 814–820. https://doi.org/10.1016/j.funbio.2020.07.003.",
pages = "820-814",
number = "9",
volume = "124",
url = "https://hdl.handle.net/21.15107/rcub_intor_640"
}

Kosanović, D., Grogan, H.,& Kavanagh, K.. (2020). Supplementary information for the article: Kosanović, D.; Grogan, H.; Kavanagh, K. Exposure of Agaricus Bisporus to Trichoderma Aggressivum f. Europaeum Leads to Growth Inhibition and Induction of an Oxidative Stress Response. Fungal Biology 2020, 124 (9), 814–820. https://doi.org/10.1016/j.funbio.2020.07.003.. in Fungal Biology, 124(9), 814-820.
https://hdl.handle.net/21.15107/rcub_intor_640

Kosanović D, Grogan H, Kavanagh K. Supplementary information for the article: Kosanović, D.; Grogan, H.; Kavanagh, K. Exposure of Agaricus Bisporus to Trichoderma Aggressivum f. Europaeum Leads to Growth Inhibition and Induction of an Oxidative Stress Response. Fungal Biology 2020, 124 (9), 814–820. https://doi.org/10.1016/j.funbio.2020.07.003.. in Fungal Biology. 2020;124(9):814-820.
https://hdl.handle.net/21.15107/rcub_intor_640 .

Kosanović, Dejana, Grogan, Helen, Kavanagh, Kevin, "Supplementary information for the article: Kosanović, D.; Grogan, H.; Kavanagh, K. Exposure of Agaricus Bisporus to Trichoderma Aggressivum f. Europaeum Leads to Growth Inhibition and Induction of an Oxidative Stress Response. Fungal Biology 2020, 124 (9), 814–820. https://doi.org/10.1016/j.funbio.2020.07.003." in Fungal Biology, 124, no. 9 (2020):814-820,
https://hdl.handle.net/21.15107/rcub_intor_640 .

TY  - JOUR
AU  - Kosanović, Dejana
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/557
AB  - Green mould disease of mushroom, Agaricus bisporus,is caused by Trichodermaspecies and can result in substantial crop losses.Label free proteomic analysis of changes in the abundance of A. bisporusproteins following exposure to T. aggressivumsupernatantin vitroindicated increased abundance of proteins associated with an oxidative stress response (zinc ion binding (+6.6 fold); peroxidase activity (5.3-fold); carboxylic ester hydrolase (+2.4 fold); dipeptidase (+3.2 fold); [2Fe-2S] cluster assembly (+3.3 fold)). Proteins that decreased in relative abundance were associated with growth: structural constituent of ribosome, translation (-12 fold), deadenylation-dependent decapping of nuclear-transcribed mRNA (-3.4 fold), and small GTPase mediated signal transduction (-2.6 fold). In vivoanalysis revealed that 10-4 T. aggressivuminoculum decreased the mushroom yield by 29% to 56% and 10-3 T. aggressivuminoculum decreased the mushroom yield by 68% to 100%. Proteins that increased in abundance in A. bisporusin vivofollowing exposure to T. aggressivumindicated an oxidative stress response and included proteins with pyruvate kinase activity (+2.6 fold) and hydrolase activity (+2.1 fold)). The results indicate that exposure of A. bisporusmycelium to T. aggressivum in vitroand in vivoresulted in an oxidative stress response and reduction in growth.
T2  - Fungal Biology
T1  - Exposure of Agaricus bisporus to Trichoderma aggressivum f. europaeum leads to growth inhibition and induction of an oxidative stress response
EP  - 820
IS  - 9
SP  - 814
VL  - 124
DO  - 10.1016/j.funbio.2020.07.003
UR  - https://hdl.handle.net/21.15107/rcub_intor_557
ER  - 

@article{
author = "Kosanović, Dejana and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Green mould disease of mushroom, Agaricus bisporus,is caused by Trichodermaspecies and can result in substantial crop losses.Label free proteomic analysis of changes in the abundance of A. bisporusproteins following exposure to T. aggressivumsupernatantin vitroindicated increased abundance of proteins associated with an oxidative stress response (zinc ion binding (+6.6 fold); peroxidase activity (5.3-fold); carboxylic ester hydrolase (+2.4 fold); dipeptidase (+3.2 fold); [2Fe-2S] cluster assembly (+3.3 fold)). Proteins that decreased in relative abundance were associated with growth: structural constituent of ribosome, translation (-12 fold), deadenylation-dependent decapping of nuclear-transcribed mRNA (-3.4 fold), and small GTPase mediated signal transduction (-2.6 fold). In vivoanalysis revealed that 10-4 T. aggressivuminoculum decreased the mushroom yield by 29% to 56% and 10-3 T. aggressivuminoculum decreased the mushroom yield by 68% to 100%. Proteins that increased in abundance in A. bisporusin vivofollowing exposure to T. aggressivumindicated an oxidative stress response and included proteins with pyruvate kinase activity (+2.6 fold) and hydrolase activity (+2.1 fold)). The results indicate that exposure of A. bisporusmycelium to T. aggressivum in vitroand in vivoresulted in an oxidative stress response and reduction in growth.",
journal = "Fungal Biology",
title = "Exposure of Agaricus bisporus to Trichoderma aggressivum f. europaeum leads to growth inhibition and induction of an oxidative stress response",
pages = "820-814",
number = "9",
volume = "124",
doi = "10.1016/j.funbio.2020.07.003",
url = "https://hdl.handle.net/21.15107/rcub_intor_557"
}

Kosanović, D., Grogan, H.,& Kavanagh, K.. (2020). Exposure of Agaricus bisporus to Trichoderma aggressivum f. europaeum leads to growth inhibition and induction of an oxidative stress response. in Fungal Biology, 124(9), 814-820.
https://doi.org/10.1016/j.funbio.2020.07.003
https://hdl.handle.net/21.15107/rcub_intor_557

Kosanović D, Grogan H, Kavanagh K. Exposure of Agaricus bisporus to Trichoderma aggressivum f. europaeum leads to growth inhibition and induction of an oxidative stress response. in Fungal Biology. 2020;124(9):814-820.
doi:10.1016/j.funbio.2020.07.003
https://hdl.handle.net/21.15107/rcub_intor_557 .

Kosanović, Dejana, Grogan, Helen, Kavanagh, Kevin, "Exposure of Agaricus bisporus to Trichoderma aggressivum f. europaeum leads to growth inhibition and induction of an oxidative stress response" in Fungal Biology, 124, no. 9 (2020):814-820,
https://doi.org/10.1016/j.funbio.2020.07.003 .,
https://hdl.handle.net/21.15107/rcub_intor_557 .

TY  - DATA
AU  - Kosanović, Dejana
AU  - Sheehan, Gerard
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/641
AB  - Figure S1. Pathogenicity assay on mushroom caps. Top of the pilei were inoculated with 20 μl of Pseudomonas tolaasii (1×1013 CFU ml−1). Sterile water was used as a negative control. Brown blotch on mushroom caps, 48h after inoculation (b), no blotch on a control (a); Figure S2. Zones of Inhibition due to P. putida (a, b) and P. tolaasii (c, d) supernatants on PDA (b, d) and ME (a, c) plates inoculated with with 104 T. aggressivum conidia and wells filled with 50 µl of 96h SN, after 48h, 30 °C; Figure S3. Comparison of superimposed P. putida and P. tolaasii supernatant chromatographs. Tolaasin Rt = 17.418 min. detected only in 96- and 120-hours P. tolaasii supernatants; Figure S4. PCA analysis. ◦ - P. putida group, ∆ - P. tolaasii group and ▫- Control group; Figure S5. Gene Ontology analysis by Blast2GO software tool. Biological process, molecular function, and cellular components significantly enriched within the proteome of P. putida/tolaasii treated T. aggressivum.
T2  - European Journal of Plant Pathology
T1  - Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.
EP  - 121
IS  - 1
SP  - 111
VL  - 156
UR  - https://hdl.handle.net/21.15107/rcub_intor_641
ER  - 

@misc{
author = "Kosanović, Dejana and Sheehan, Gerard and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Figure S1. Pathogenicity assay on mushroom caps. Top of the pilei were inoculated with 20 μl of Pseudomonas tolaasii (1×1013 CFU ml−1). Sterile water was used as a negative control. Brown blotch on mushroom caps, 48h after inoculation (b), no blotch on a control (a); Figure S2. Zones of Inhibition due to P. putida (a, b) and P. tolaasii (c, d) supernatants on PDA (b, d) and ME (a, c) plates inoculated with with 104 T. aggressivum conidia and wells filled with 50 µl of 96h SN, after 48h, 30 °C; Figure S3. Comparison of superimposed P. putida and P. tolaasii supernatant chromatographs. Tolaasin Rt = 17.418 min. detected only in 96- and 120-hours P. tolaasii supernatants; Figure S4. PCA analysis. ◦ - P. putida group, ∆ - P. tolaasii group and ▫- Control group; Figure S5. Gene Ontology analysis by Blast2GO software tool. Biological process, molecular function, and cellular components significantly enriched within the proteome of P. putida/tolaasii treated T. aggressivum.",
journal = "European Journal of Plant Pathology",
title = "Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.",
pages = "121-111",
number = "1",
volume = "156",
url = "https://hdl.handle.net/21.15107/rcub_intor_641"
}

Kosanović, D., Sheehan, G., Grogan, H.,& Kavanagh, K.. (2020). Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.. in European Journal of Plant Pathology, 156(1), 111-121.
https://hdl.handle.net/21.15107/rcub_intor_641

Kosanović D, Sheehan G, Grogan H, Kavanagh K. Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.. in European Journal of Plant Pathology. 2020;156(1):111-121.
https://hdl.handle.net/21.15107/rcub_intor_641 .

Kosanović, Dejana, Sheehan, Gerard, Grogan, Helen, Kavanagh, Kevin, "Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z." in European Journal of Plant Pathology, 156, no. 1 (2020):111-121,
https://hdl.handle.net/21.15107/rcub_intor_641 .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Kosanović, Dejana
AU  - Molnar, Zsuzsanna
AU  - Begović, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Jeney, Galina
AU  - Ljubobratović, Uroš
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/529
AB  - The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracaseisubsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed
IS  - 7
VL  - 14
DO  - 10.1371/journal.pone.0219558
ER  - 

@article{
author = "Lukić, Jovanka and Vukotić, Goran and Stanisavljević, Nemanja and Kosanović, Dejana and Molnar, Zsuzsanna and Begović, Jelena and Terzić-Vidojević, Amarela and Jeney, Galina and Ljubobratović, Uroš",
year = "2019",
abstract = "The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracaseisubsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed",
number = "7",
volume = "14",
doi = "10.1371/journal.pone.0219558"
}

Lukić, J., Vukotić, G., Stanisavljević, N., Kosanović, D., Molnar, Z., Begović, J., Terzić-Vidojević, A., Jeney, G.,& Ljubobratović, U.. (2019). Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed. in PLoS One
Public Library Science, San Francisco., 14(7).
https://doi.org/10.1371/journal.pone.0219558

Lukić J, Vukotić G, Stanisavljević N, Kosanović D, Molnar Z, Begović J, Terzić-Vidojević A, Jeney G, Ljubobratović U. Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed. in PLoS One. 2019;14(7).
doi:10.1371/journal.pone.0219558 .

Lukić, Jovanka, Vukotić, Goran, Stanisavljević, Nemanja, Kosanović, Dejana, Molnar, Zsuzsanna, Begović, Jelena, Terzić-Vidojević, Amarela, Jeney, Galina, Ljubobratović, Uroš, "Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed" in PLoS One, 14, no. 7 (2019),
https://doi.org/10.1371/journal.pone.0219558 . .

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stein, Elisabeth
AU  - Stojanović, Marijana
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Filipović, Ana
AU  - Marinković, Emilija
AU  - Kosanović, Dejana
AU  - Barisani-Asenbauer, Talin
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/502
AB  - Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.
PB  - Springer, Dordrecht
T2  - Journal of Applied Phycology
T1  - Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo
EP  - 2610
IS  - 4
SP  - 2601
VL  - 30
DO  - 10.1007/s10811-018-1435-0
ER  - 

@article{
author = "Inić-Kanada, Aleksandra and Stein, Elisabeth and Stojanović, Marijana and Schuerer, Nadine and Ghasemian, Ehsan and Filipović, Ana and Marinković, Emilija and Kosanović, Dejana and Barisani-Asenbauer, Talin",
year = "2018",
abstract = "Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.",
publisher = "Springer, Dordrecht",
journal = "Journal of Applied Phycology",
title = "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo",
pages = "2610-2601",
number = "4",
volume = "30",
doi = "10.1007/s10811-018-1435-0"
}

Inić-Kanada, A., Stein, E., Stojanović, M., Schuerer, N., Ghasemian, E., Filipović, A., Marinković, E., Kosanović, D.,& Barisani-Asenbauer, T.. (2018). Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology
Springer, Dordrecht., 30(4), 2601-2610.
https://doi.org/10.1007/s10811-018-1435-0

Inić-Kanada A, Stein E, Stojanović M, Schuerer N, Ghasemian E, Filipović A, Marinković E, Kosanović D, Barisani-Asenbauer T. Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology. 2018;30(4):2601-2610.
doi:10.1007/s10811-018-1435-0 .

Inić-Kanada, Aleksandra, Stein, Elisabeth, Stojanović, Marijana, Schuerer, Nadine, Ghasemian, Ehsan, Filipović, Ana, Marinković, Emilija, Kosanović, Dejana, Barisani-Asenbauer, Talin, "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo" in Journal of Applied Phycology, 30, no. 4 (2018):2601-2610,
https://doi.org/10.1007/s10811-018-1435-0 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Stojković, Aleksandra
AU  - Kosanović, Dejana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Jovanova-Nešić, Katica
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/509
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis
T1  - Vakcinacija i autoimunost - vakcina protiv gripa i povezanost sa multiplom sklerozom
EP  - 729
IS  - 7
SP  - 721
VL  - 75
DO  - 10.2298/VSP160620002M
ER  - 

@article{
author = "Maslovarić, Irina and Stojković, Aleksandra and Kosanović, Dejana and Marković, Dragana and Ilić, Vesna and Jovanova-Nešić, Katica",
year = "2018",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis, Vakcinacija i autoimunost - vakcina protiv gripa i povezanost sa multiplom sklerozom",
pages = "729-721",
number = "7",
volume = "75",
doi = "10.2298/VSP160620002M"
}

Maslovarić, I., Stojković, A., Kosanović, D., Marković, D., Ilić, V.,& Jovanova-Nešić, K.. (2018). Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 75(7), 721-729.
https://doi.org/10.2298/VSP160620002M

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis. in Vojnosanitetski pregled. 2018;75(7):721-729.
doi:10.2298/VSP160620002M .

Maslovarić, Irina, Stojković, Aleksandra, Kosanović, Dejana, Marković, Dragana, Ilić, Vesna, Jovanova-Nešić, Katica, "Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis" in Vojnosanitetski pregled, 75, no. 7 (2018):721-729,
https://doi.org/10.2298/VSP160620002M . .

TY  - JOUR
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Lukić, Ivana
AU  - Filipović, Ana
AU  - Inić-Kanada, Aleksandra
AU  - Kosanović, Dejana
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/493
AB  - We demonstrated that a recombinant banana lectin (rBanLec), which structural characteristics and physiological impacts highly resemble those reported for its natural counterparts, binds murine peritoneal macrophages and specifically modulates their functional characteristics. By using rBanLec in concentrations ranging from 1 mu g to 10 mu g to stimulate resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages from BALB/c and C57BL/6 mice, we have shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages and their genetic background. rBanLec, in a positive dose-dependent manner, promotes the proliferation of TGMs from both BALB/c and C57BL/6 mice, while its mitogenic influence on RMs is significantly lower (BALB/c mice) or not detectable (C57BL/6 mice). In all peritoneal macrophages, irrespective of their type and genetic background, rBanLec, in a positive dose dependent manner, enhances the secretion of IL-10. rBanLec stimulation of RMs from both BALB/c and C57BL/6 resulted in a positive dose-dependent promotion of proinflammatory phenotype (enhancement of NO production and IL-12 and TNF alpha secretion, reduction of arginase activity). Positive dose-dependent skewing toward proinflammatory phenotype was also observed in TGMs from C57BL/6 mice. However, the enhancement of rBanLec stimulation promotes skewing of TGMs from BALB/c mice towards anti-inflammatory profile (reduction of NO production and IL-12 secretion, enhancement of arginase activity and TGF alpha and IL-4 secretion). Moreover, we established that rBanLec binds oligosaccharide structures of TLR2 and CD14 and that blocking of signaling via these receptors significantly impairs the production of TNFa and NO in BALB/c macrophages. Since the outcome of rBanLec stimulation depends on rBanLec concentration as well as on the functional characteristics of its target cells and their genetic background, further studies are needed to investigate its effects under physiological and specific pathological conditions.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin
IS  - 2
VL  - 12
DO  - 10.1371/journal.pone.0172469
ER  - 

@article{
author = "Marinković, Emilija and Đokić, Radmila and Lukić, Ivana and Filipović, Ana and Inić-Kanada, Aleksandra and Kosanović, Dejana and Gavrović-Jankulović, Marija and Stojanović, Marijana",
year = "2017",
abstract = "We demonstrated that a recombinant banana lectin (rBanLec), which structural characteristics and physiological impacts highly resemble those reported for its natural counterparts, binds murine peritoneal macrophages and specifically modulates their functional characteristics. By using rBanLec in concentrations ranging from 1 mu g to 10 mu g to stimulate resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages from BALB/c and C57BL/6 mice, we have shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages and their genetic background. rBanLec, in a positive dose-dependent manner, promotes the proliferation of TGMs from both BALB/c and C57BL/6 mice, while its mitogenic influence on RMs is significantly lower (BALB/c mice) or not detectable (C57BL/6 mice). In all peritoneal macrophages, irrespective of their type and genetic background, rBanLec, in a positive dose dependent manner, enhances the secretion of IL-10. rBanLec stimulation of RMs from both BALB/c and C57BL/6 resulted in a positive dose-dependent promotion of proinflammatory phenotype (enhancement of NO production and IL-12 and TNF alpha secretion, reduction of arginase activity). Positive dose-dependent skewing toward proinflammatory phenotype was also observed in TGMs from C57BL/6 mice. However, the enhancement of rBanLec stimulation promotes skewing of TGMs from BALB/c mice towards anti-inflammatory profile (reduction of NO production and IL-12 secretion, enhancement of arginase activity and TGF alpha and IL-4 secretion). Moreover, we established that rBanLec binds oligosaccharide structures of TLR2 and CD14 and that blocking of signaling via these receptors significantly impairs the production of TNFa and NO in BALB/c macrophages. Since the outcome of rBanLec stimulation depends on rBanLec concentration as well as on the functional characteristics of its target cells and their genetic background, further studies are needed to investigate its effects under physiological and specific pathological conditions.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin",
number = "2",
volume = "12",
doi = "10.1371/journal.pone.0172469"
}

Marinković, E., Đokić, R., Lukić, I., Filipović, A., Inić-Kanada, A., Kosanović, D., Gavrović-Jankulović, M.,& Stojanović, M.. (2017). Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin. in PLoS One
Public Library Science, San Francisco., 12(2).
https://doi.org/10.1371/journal.pone.0172469

Marinković E, Đokić R, Lukić I, Filipović A, Inić-Kanada A, Kosanović D, Gavrović-Jankulović M, Stojanović M. Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin. in PLoS One. 2017;12(2).
doi:10.1371/journal.pone.0172469 .

Marinković, Emilija, Đokić, Radmila, Lukić, Ivana, Filipović, Ana, Inić-Kanada, Aleksandra, Kosanović, Dejana, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin" in PLoS One, 12, no. 2 (2017),
https://doi.org/10.1371/journal.pone.0172469 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Stojanović, Marijana
AU  - Stein, Elisabeth
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Đokić, Radmila
AU  - Kosanović, Dejana
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/486
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.
IS  - 8
VL  - 58
UR  - https://hdl.handle.net/21.15107/rcub_intor_486
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Lukić, Ivana and Stojanović, Marijana and Stein, Elisabeth and Marinković, Emilija and Filipović, Ana and Đokić, Radmila and Kosanović, Dejana and Schuerer, Nadine and Ghasemian, Ehsan and Barisani-Asenbauer, Talin",
year = "2017",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.",
number = "8",
volume = "58",
url = "https://hdl.handle.net/21.15107/rcub_intor_486"
}

Inić-Kanada, A., Lukić, I., Stojanović, M., Stein, E., Marinković, E., Filipović, A., Đokić, R., Kosanović, D., Schuerer, N., Ghasemian, E.,& Barisani-Asenbauer, T.. (2017). Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 58(8).
https://hdl.handle.net/21.15107/rcub_intor_486

Inić-Kanada A, Lukić I, Stojanović M, Stein E, Marinković E, Filipović A, Đokić R, Kosanović D, Schuerer N, Ghasemian E, Barisani-Asenbauer T. Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.. in Investigative Ophthalmology & Visual Science. 2017;58(8).
https://hdl.handle.net/21.15107/rcub_intor_486 .

Inić-Kanada, Aleksandra, Lukić, Ivana, Stojanović, Marijana, Stein, Elisabeth, Marinković, Emilija, Filipović, Ana, Đokić, Radmila, Kosanović, Dejana, Schuerer, Nadine, Ghasemian, Ehsan, Barisani-Asenbauer, Talin, "Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection." in Investigative Ophthalmology & Visual Science, 58, no. 8 (2017),
https://hdl.handle.net/21.15107/rcub_intor_486 .

TY  - CONF
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Filipović, Ana
AU  - Lukić, Ivana
AU  - Kosanović, Dejana
AU  - Inić-Kanada, Aleksandra
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/478
PB  - Wiley, Hoboken
C3  - FEBS Journal
T1  - Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice
EP  - 125
SP  - 124
VL  - 284
UR  - https://hdl.handle.net/21.15107/rcub_intor_478
ER  - 

@conference{
author = "Marinković, Emilija and Đokić, Radmila and Filipović, Ana and Lukić, Ivana and Kosanović, Dejana and Inić-Kanada, Aleksandra and Gavrović-Jankulović, Marija and Stojanović, Marijana",
year = "2017",
publisher = "Wiley, Hoboken",
journal = "FEBS Journal",
title = "Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice",
pages = "125-124",
volume = "284",
url = "https://hdl.handle.net/21.15107/rcub_intor_478"
}

Marinković, E., Đokić, R., Filipović, A., Lukić, I., Kosanović, D., Inić-Kanada, A., Gavrović-Jankulović, M.,& Stojanović, M.. (2017). Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice. in FEBS Journal
Wiley, Hoboken., 284, 124-125.
https://hdl.handle.net/21.15107/rcub_intor_478

Marinković E, Đokić R, Filipović A, Lukić I, Kosanović D, Inić-Kanada A, Gavrović-Jankulović M, Stojanović M. Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice. in FEBS Journal. 2017;284:124-125.
https://hdl.handle.net/21.15107/rcub_intor_478 .

Marinković, Emilija, Đokić, Radmila, Filipović, Ana, Lukić, Ivana, Kosanović, Dejana, Inić-Kanada, Aleksandra, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice" in FEBS Journal, 284 (2017):124-125,
https://hdl.handle.net/21.15107/rcub_intor_478 .

TY  - JOUR
AU  - Filipović, Ana
AU  - Ghasemian, Ehsan
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Stein, Elisabeth
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Kosanović, Dejana
AU  - Schuerer, Nadine
AU  - Chalabi, Hadeel
AU  - Belij-Rammerstorfer, Sandra
AU  - Stojanović, Marijana
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/477
AB  - Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection
IS  - 7
VL  - 12
DO  - 10.1371/journal.pone.0180551
ER  - 

@article{
author = "Filipović, Ana and Ghasemian, Ehsan and Inić-Kanada, Aleksandra and Lukić, Ivana and Stein, Elisabeth and Marinković, Emilija and Đokić, Radmila and Kosanović, Dejana and Schuerer, Nadine and Chalabi, Hadeel and Belij-Rammerstorfer, Sandra and Stojanović, Marijana and Barisani-Asenbauer, Talin",
year = "2017",
abstract = "Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection",
number = "7",
volume = "12",
doi = "10.1371/journal.pone.0180551"
}

Filipović, A., Ghasemian, E., Inić-Kanada, A., Lukić, I., Stein, E., Marinković, E., Đokić, R., Kosanović, D., Schuerer, N., Chalabi, H., Belij-Rammerstorfer, S., Stojanović, M.,& Barisani-Asenbauer, T.. (2017). The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection. in PLoS One
Public Library Science, San Francisco., 12(7).
https://doi.org/10.1371/journal.pone.0180551

Filipović A, Ghasemian E, Inić-Kanada A, Lukić I, Stein E, Marinković E, Đokić R, Kosanović D, Schuerer N, Chalabi H, Belij-Rammerstorfer S, Stojanović M, Barisani-Asenbauer T. The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection. in PLoS One. 2017;12(7).
doi:10.1371/journal.pone.0180551 .

Filipović, Ana, Ghasemian, Ehsan, Inić-Kanada, Aleksandra, Lukić, Ivana, Stein, Elisabeth, Marinković, Emilija, Đokić, Radmila, Kosanović, Dejana, Schuerer, Nadine, Chalabi, Hadeel, Belij-Rammerstorfer, Sandra, Stojanović, Marijana, Barisani-Asenbauer, Talin, "The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection" in PLoS One, 12, no. 7 (2017),
https://doi.org/10.1371/journal.pone.0180551 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Stojković, A.
AU  - Kosanović, Dejana
AU  - Marković, D.
AU  - Ilić, Vesna
AU  - Jovanova-Nešić, Katica
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/499
AB  - The names of the authors were indicated incorrectly (wrong using of diacritical marks). The names of the authors should be written and read as follows: I. Maslovarić, A. Stojković, D. Kosanović, D. Marković, V. Ilić, and K. Jovanova-Nešić.
PB  - Springer New York LLC
T2  - Neurophysiology
T1  - Correction to: Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (Neurophysiology, (2017), 49, 4, (276-282), 10.1007/s11062-017-9682-2)
IS  - 5
SP  - 393
VL  - 49
DO  - 10.1007/s11062-018-9699-1
ER  - 

@article{
author = "Maslovarić, Irina and Stojković, A. and Kosanović, Dejana and Marković, D. and Ilić, Vesna and Jovanova-Nešić, Katica",
year = "2017",
abstract = "The names of the authors were indicated incorrectly (wrong using of diacritical marks). The names of the authors should be written and read as follows: I. Maslovarić, A. Stojković, D. Kosanović, D. Marković, V. Ilić, and K. Jovanova-Nešić.",
publisher = "Springer New York LLC",
journal = "Neurophysiology",
title = "Correction to: Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (Neurophysiology, (2017), 49, 4, (276-282), 10.1007/s11062-017-9682-2)",
number = "5",
pages = "393",
volume = "49",
doi = "10.1007/s11062-018-9699-1"
}

Maslovarić, I., Stojković, A., Kosanović, D., Marković, D., Ilić, V.,& Jovanova-Nešić, K.. (2017). Correction to: Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (Neurophysiology, (2017), 49, 4, (276-282), 10.1007/s11062-017-9682-2). in Neurophysiology
Springer New York LLC., 49(5), 393.
https://doi.org/10.1007/s11062-018-9699-1

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. Correction to: Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (Neurophysiology, (2017), 49, 4, (276-282), 10.1007/s11062-017-9682-2). in Neurophysiology. 2017;49(5):393.
doi:10.1007/s11062-018-9699-1 .

Maslovarić, Irina, Stojković, A., Kosanović, Dejana, Marković, D., Ilić, Vesna, Jovanova-Nešić, Katica, "Correction to: Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (Neurophysiology, (2017), 49, 4, (276-282), 10.1007/s11062-017-9682-2)" in Neurophysiology, 49, no. 5 (2017):393,
https://doi.org/10.1007/s11062-018-9699-1 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Stojković, A.
AU  - Kosanović, Dejana
AU  - Marković, D.
AU  - Ilić, Vesna
AU  - Jovanova-Nešić, Katica
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/488
AB  - We examined the accessibility of influenza virus and diphtheria-tetanus toxin (DiTe) antigens to the choroid plexus (CP) within the postvaccination period and the expression of CD11b molecules (by immunohistochemistry). Eighteen Dark Agouti (DA) rats were divided into three groups: (i) animals administered with influenza vaccine (Flu), (ii) animals administered with DiTe vaccine (DiTe), and (iii) nontreated (Contr) animals. The serum antibody titers following influenza and diphtheria-tetanus vaccination were detected by the ELISA test. Immunohistochemical staining revealed a great number of viral antigen-positive and CD11b-positive brain cells in Flu rats compared to a very small number of the respective cells in DiTe animals and no staining in the Contr group. DiTe- and Flu-rats showed significant increases in the serum anti-tetanus toxoid and anti-influenza virus antibody levels compared to those in the Contr group. The results obtained attract attention towards the dynamic role of the CP in the immunosurveillance of the CNS. Based on the viral antigen deposits accumulated in the CP, it has been proposed that the latter can play an active role in modulation of the immune response after influenza vaccine immunization.
PB  - Springer, New York
T2  - Neurophysiology
T1  - Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus
EP  - 282
IS  - 4
SP  - 276
VL  - 49
DO  - 10.1007/s11062-017-9682-2
ER  - 

@article{
author = "Maslovarić, Irina and Stojković, A. and Kosanović, Dejana and Marković, D. and Ilić, Vesna and Jovanova-Nešić, Katica",
year = "2017",
abstract = "We examined the accessibility of influenza virus and diphtheria-tetanus toxin (DiTe) antigens to the choroid plexus (CP) within the postvaccination period and the expression of CD11b molecules (by immunohistochemistry). Eighteen Dark Agouti (DA) rats were divided into three groups: (i) animals administered with influenza vaccine (Flu), (ii) animals administered with DiTe vaccine (DiTe), and (iii) nontreated (Contr) animals. The serum antibody titers following influenza and diphtheria-tetanus vaccination were detected by the ELISA test. Immunohistochemical staining revealed a great number of viral antigen-positive and CD11b-positive brain cells in Flu rats compared to a very small number of the respective cells in DiTe animals and no staining in the Contr group. DiTe- and Flu-rats showed significant increases in the serum anti-tetanus toxoid and anti-influenza virus antibody levels compared to those in the Contr group. The results obtained attract attention towards the dynamic role of the CP in the immunosurveillance of the CNS. Based on the viral antigen deposits accumulated in the CP, it has been proposed that the latter can play an active role in modulation of the immune response after influenza vaccine immunization.",
publisher = "Springer, New York",
journal = "Neurophysiology",
title = "Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus",
pages = "282-276",
number = "4",
volume = "49",
doi = "10.1007/s11062-017-9682-2"
}

Maslovarić, I., Stojković, A., Kosanović, D., Marković, D., Ilić, V.,& Jovanova-Nešić, K.. (2017). Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus. in Neurophysiology
Springer, New York., 49(4), 276-282.
https://doi.org/10.1007/s11062-017-9682-2

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus. in Neurophysiology. 2017;49(4):276-282.
doi:10.1007/s11062-017-9682-2 .

Maslovarić, Irina, Stojković, A., Kosanović, Dejana, Marković, D., Ilić, Vesna, Jovanova-Nešić, Katica, "Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus" in Neurophysiology, 49, no. 4 (2017):276-282,
https://doi.org/10.1007/s11062-017-9682-2 . .

TY  - JOUR
AU  - Ljubobratović, Uroš
AU  - Kosanović, Dejana
AU  - Vukotić, Goran
AU  - Molnar, Zsuzsanna
AU  - Stanisavljević, Nemanja
AU  - Ristović, Tijana
AU  - Peter, Geza
AU  - Lukić, Jovanka
AU  - Jeney, Galina
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/483
AB  - This research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed OTOHIME fish diet, or through Artemia nauplii, on fish growth, microbiota balance and skeletal development. On the 12th Day Post Hatching (DPH) fish were divided into following treatment groups: two groups received the combination of OTOHIME and nauplii enriched either with Lactobacillus paracasei BGHN14 + Lactobacillus rhamnosus BGTH or with Lactobacillus reuteri BGGO6-55 + Lactobacillus salivarius BGHO1, and one group received OTOHIME hydrolyzed by BGHN14 + BGT10 and non-enriched nauplii. Control group received non-enriched nauplii and non-hydrolyzed OTOHIME. The treatment lasted 14 days and fish were sacrificed on the 26th DPH for the assessment of digestive enzyme activity and microbiota composition. Individual total lengths and individual body weights were recorded at the end of the treatments, on the 26th DPH, and also on the 45th DPH, in parallel with the evaluation of skeletal deformities and fish survival. Our results indicated positive effect of Anemia enriched with BGGO6-55 + BGHO1 on fish growth, skeletal development and trypsin to chymotrypsin activity ratio (T/C), as an indicator of protein digestibility. Hydrolysis of OTOHIME was also associated with better skeletal development, higher T/C values and lower levels of Aeromonas and Mycobacterium spp., which are important fish pathogens. Though additional testing in larger cohort studies is needed, these observations are promising in terms of usage of probiotics for improved environmentally friendly production of pike-perch in Recirculating Aquaculture System (RAS).
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study
EP  - 462
SP  - 451
VL  - 115
DO  - 10.1016/j.rvsc.2017.07.018
ER  - 

@article{
author = "Ljubobratović, Uroš and Kosanović, Dejana and Vukotić, Goran and Molnar, Zsuzsanna and Stanisavljević, Nemanja and Ristović, Tijana and Peter, Geza and Lukić, Jovanka and Jeney, Galina",
year = "2017",
abstract = "This research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed OTOHIME fish diet, or through Artemia nauplii, on fish growth, microbiota balance and skeletal development. On the 12th Day Post Hatching (DPH) fish were divided into following treatment groups: two groups received the combination of OTOHIME and nauplii enriched either with Lactobacillus paracasei BGHN14 + Lactobacillus rhamnosus BGTH or with Lactobacillus reuteri BGGO6-55 + Lactobacillus salivarius BGHO1, and one group received OTOHIME hydrolyzed by BGHN14 + BGT10 and non-enriched nauplii. Control group received non-enriched nauplii and non-hydrolyzed OTOHIME. The treatment lasted 14 days and fish were sacrificed on the 26th DPH for the assessment of digestive enzyme activity and microbiota composition. Individual total lengths and individual body weights were recorded at the end of the treatments, on the 26th DPH, and also on the 45th DPH, in parallel with the evaluation of skeletal deformities and fish survival. Our results indicated positive effect of Anemia enriched with BGGO6-55 + BGHO1 on fish growth, skeletal development and trypsin to chymotrypsin activity ratio (T/C), as an indicator of protein digestibility. Hydrolysis of OTOHIME was also associated with better skeletal development, higher T/C values and lower levels of Aeromonas and Mycobacterium spp., which are important fish pathogens. Though additional testing in larger cohort studies is needed, these observations are promising in terms of usage of probiotics for improved environmentally friendly production of pike-perch in Recirculating Aquaculture System (RAS).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study",
pages = "462-451",
volume = "115",
doi = "10.1016/j.rvsc.2017.07.018"
}

Ljubobratović, U., Kosanović, D., Vukotić, G., Molnar, Z., Stanisavljević, N., Ristović, T., Peter, G., Lukić, J.,& Jeney, G.. (2017). Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 115, 451-462.
https://doi.org/10.1016/j.rvsc.2017.07.018

Ljubobratović U, Kosanović D, Vukotić G, Molnar Z, Stanisavljević N, Ristović T, Peter G, Lukić J, Jeney G. Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study. in Research in Veterinary Science. 2017;115:451-462.
doi:10.1016/j.rvsc.2017.07.018 .

Ljubobratović, Uroš, Kosanović, Dejana, Vukotić, Goran, Molnar, Zsuzsanna, Stanisavljević, Nemanja, Ristović, Tijana, Peter, Geza, Lukić, Jovanka, Jeney, Galina, "Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study" in Research in Veterinary Science, 115 (2017):451-462,
https://doi.org/10.1016/j.rvsc.2017.07.018 . .

TY  - JOUR
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Kosanović, Dejana
AU  - Inić-Kanada, Aleksandra
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/471
AB  - Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon
EP  - 78
SP  - 68
VL  - 20
DO  - 10.1016/j.jff.2015.10.019
ER  - 

@article{
author = "Marinković, Emilija and Lukić, Ivana and Kosanović, Dejana and Inić-Kanada, Aleksandra and Gavrović-Jankulović, Marija and Stojanović, Marijana",
year = "2016",
abstract = "Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon",
pages = "78-68",
volume = "20",
doi = "10.1016/j.jff.2015.10.019"
}

Marinković, E., Lukić, I., Kosanović, D., Inić-Kanada, A., Gavrović-Jankulović, M.,& Stojanović, M.. (2016). Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon. in Journal of Functional Foods
Elsevier, Amsterdam., 20, 68-78.
https://doi.org/10.1016/j.jff.2015.10.019

Marinković E, Lukić I, Kosanović D, Inić-Kanada A, Gavrović-Jankulović M, Stojanović M. Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon. in Journal of Functional Foods. 2016;20:68-78.
doi:10.1016/j.jff.2015.10.019 .

Marinković, Emilija, Lukić, Ivana, Kosanović, Dejana, Inić-Kanada, Aleksandra, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon" in Journal of Functional Foods, 20 (2016):68-78,
https://doi.org/10.1016/j.jff.2015.10.019 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Krnjaja, Ognjen
AU  - Kosanović, Dejana
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/425
AB  - Antibodies capable to neutralize tetanus toxin (TeNT) are key factors in protection against tetanus disease. Although antibody-based therapeutics for treatment of tetanus exist on the market its production is tedious. Hence, the tetanus-specific antibodies preparation that could be easily produced in large scale in vitro would be beneficial. Monoclonal antibodies (MAbs) are considered for a long time as a reagent of choice, but the core drawback is how to select a MAb that would be safe in providing efficacious protection. In this study we have investigated the parameters crucial for a single MAb to be assigned as protective. Eight murine MAbs were characterized in vitro for their reactivity toward TeNT and assessed in vivo for protectiveness against TeNT intoxication. Correlation of in vitro and in vivo data has revealed that in vitro selection of MAb that is protective in vivo could be performed by a combination of two assays: the measurement of MAb affinity toward TeNT taking Ka 1 x 10(8) M-1 as a threshold level, and the evaluation of its capability to prevent TeNT-ganglioside interaction. Single MAb could be taken into consideration as a potential therapeutic only if it has a capacity to completely inhibits TeNT-ganglioside complex formation. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicon
T1  - Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction
EP  - 144
SP  - 135
VL  - 103
DO  - 10.1016/j.toxicon.2015.06.025
ER  - 

@article{
author = "Lukić, Ivana and Marinković, Emilija and Filipović, Ana and Krnjaja, Ognjen and Kosanović, Dejana and Inić-Kanada, Aleksandra and Stojanović, Marijana",
year = "2015",
abstract = "Antibodies capable to neutralize tetanus toxin (TeNT) are key factors in protection against tetanus disease. Although antibody-based therapeutics for treatment of tetanus exist on the market its production is tedious. Hence, the tetanus-specific antibodies preparation that could be easily produced in large scale in vitro would be beneficial. Monoclonal antibodies (MAbs) are considered for a long time as a reagent of choice, but the core drawback is how to select a MAb that would be safe in providing efficacious protection. In this study we have investigated the parameters crucial for a single MAb to be assigned as protective. Eight murine MAbs were characterized in vitro for their reactivity toward TeNT and assessed in vivo for protectiveness against TeNT intoxication. Correlation of in vitro and in vivo data has revealed that in vitro selection of MAb that is protective in vivo could be performed by a combination of two assays: the measurement of MAb affinity toward TeNT taking Ka 1 x 10(8) M-1 as a threshold level, and the evaluation of its capability to prevent TeNT-ganglioside interaction. Single MAb could be taken into consideration as a potential therapeutic only if it has a capacity to completely inhibits TeNT-ganglioside complex formation. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicon",
title = "Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction",
pages = "144-135",
volume = "103",
doi = "10.1016/j.toxicon.2015.06.025"
}

Lukić, I., Marinković, E., Filipović, A., Krnjaja, O., Kosanović, D., Inić-Kanada, A.,& Stojanović, M.. (2015). Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction. in Toxicon
Pergamon-Elsevier Science Ltd, Oxford., 103, 135-144.
https://doi.org/10.1016/j.toxicon.2015.06.025

Lukić I, Marinković E, Filipović A, Krnjaja O, Kosanović D, Inić-Kanada A, Stojanović M. Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction. in Toxicon. 2015;103:135-144.
doi:10.1016/j.toxicon.2015.06.025 .

Lukić, Ivana, Marinković, Emilija, Filipović, Ana, Krnjaja, Ognjen, Kosanović, Dejana, Inić-Kanada, Aleksandra, Stojanović, Marijana, "Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction" in Toxicon, 103 (2015):135-144,
https://doi.org/10.1016/j.toxicon.2015.06.025 . .

TY  - THES
AU  - Kosanović, Dejana
PY  - 2015
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2722
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10857/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47617039
UR  - http://nardus.mpn.gov.rs/handle/123456789/5088
UR  - http://intor.torlakinstitut.com/handle/123456789/574
AB  - Trichoderma species, the causal agents of green mould disease, induce great losses in button mushroom (Agaricus bisporus) farms. Twenty Trichoderma isolates were collected on 13 Serbian A. bisporus farms and one in Bosnia and Herzegovina during 2006 – 2010. Twelve isolates were classified into five species by standard mycological studies and ITS1/ITS4 sequence analyses, namely Trichoderma atroviride, Trichoderma koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum and Trichoderma harzianum. Eight isolates were not identified to the species level but were shown to be related to T. harzianum. The isolates of T. harzianum exhibited the highest virulence to the harvested A. bisporus pilei and T. virens and T. aggressivum f. europaeum the lowest. Fungicides are widely used to control mushroom diseases although green mould control is encumbered with difficulties. The aims of this study were therefore to research in vitro toxicity of several commercial fungicides to Trichoderma isolates originating from Serbian and Bosnia-Herzegovina farms, and to evaluate the effects of pH and light on their growth. The majority of isolates demonstrated optimal growth at pH 5.0, and the rest at pH 6.0. A few isolates also grew well at pH 7. The weakest mycelial growth was noted at pH 8.0 – 9.0. Generally, light had an inhibitory effect on the growth of tested isolates. The isolates showed the highest susceptibility to prochloraz-Mn, chlorothalonil and carbendazim (ED50 less than 1 mg/L), and were less sensitive to iprodione (ED50 ranged 0.84 – 6.72 mg/L), weakly resistant to thiophanate-methyl (ED50 = 3.75 – 24.13 mg/L), and resistant to trifloxystrobin (ED50 = 10.25 – 178.23 mg/L). Considering the toxicity of fungicides to A. bisporus, prochloraz-Mn and carbendazim showed the best selective toxicity (0.05 and 0.02 respectively), iprodione and chlorothalonil moderate (0.16), and thiophanate-methyl the lowest (1.24), while trifloxystrobin toxicity to A. bisporus was not tested because of its inefficiency against Trichoderma isolates. The best selective toxicity to both pathogen and the host showed prochloraz-Mn and carbendazim with values less than 0.1. Carbendazim was withdrawn from the market and therefore prochloraz-Mn could be recommended for mushroom cultivation in Serbia. Antifungal activity of two biofungicides based on Bacillus subtilis and tea tree oil were tested in vitro to all Trichoderma isolates. B. subtilis was highly toxic to all tested Trichoderma isolates, their ED50 values were below 1.3 mg/L. Tea tree oil did not exhibit a significant antifungal activity (ED50 = 11.9 – 370.8 mg/L). The efficacy of biofungicides was evaluated against T. harzianum in a mushroom growing room, and they were applied alone or in combination with the fungicide at a respective proportion of 20:80%. Prochloraz-Mn showed higher efficacy than both tested biofungicides or their respective mixtures. The biofungicide based on B. subtilis demonstrated greater efficacy in preventing disease symptoms than tea tree oil. B. subtilis combined with the fungicide revealed less antagonism in efficacy against pathogen than tea tree oil.
AB  - Pojedine vrste roda Trichoderma su uzročnici zelene plesni šampinjona (Agaricus bisporus) i nanose velike materijalne štete u gajilištima. Od 2006. do 2010. godine prikupljeno je 20 izolata roda Trichoderma iz 13 gajilišta u Srbiji i jednog iz Bosne i Hercegovine. Dvanaest izolata je klasifikovano na osnovu standardnih mikoloških metoda i analizom ITS1/ITS4 sekvence u pet vrsta: Trichoderma atroviride, Trichoderma koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum i Trichoderma harzianum. Osam izolata nije identifikovano do nivoa vrste ali je pokazano da su filogenetski bliski sa vrstom T. harzianum. Najvišu virulentnost na ubranom šeširu A. bisporus je pokazala vrsta T. harzianum, a najnižu T. virens i T. aggressivum f. europaeum. Većina izolata je najbolje rasla na podlozi koja je imala pH = 5, potom na pH = 6, par izolata na pH = 7, dok je najslabiji rast zabeležen na pH = 8 – 9. Generalno, svetlost je imala inhibitorni efekat na rast izolata. Fungicidi su u širokoj upotrebi u kontroli bolesti šampinjona, iako je kontrola zelene plesni otežana. Stoga, jedan od ciljeva istraživanja je bio da se ispita in vitro toksičnost nekoliko dostupnih komercijalnih fungicida na prikupljene izolate roda Trichoderma. Testirani izolati su bili najosetljivi na prohloraz-Mn, hlorotalonil i karbendazim (EC50  1 mg/L), osetljivi na iprodion (EC50 = 0,84 – 6,72 mg/L), umereno rezistentni na tiofanat-metil (EC50 = 3,75 – 24,13 mg/L) i rezistentni na trifloksistrobin (EC50 = 10,25 – 178,23 mg/L). Proučavajući toksičnost fungicida na A. bisporus, najbolju selektivnu toksičnost su pokazali prohloraz-Mn (0,05) i karbendazim (0,02), iprodion i hlorotalonil umerenu (0,16), tiofanat-metil najnižu (1,24), dok toksičnost trifloksistrobina za A. bisporus nije testirana zbog nezadovoljavajuće toksičnosti za Trichoderma spp. izolate. Najbolju selektivnu toksičnost za patogenu gljivu i gljivu domaćina su pokazali prohloraz-Mn i karbendazim sa vrednostima nižim od 0,1. Pošto se karbendazim povlači sa tržišta, prohloraz-Mn je fungicid koji se može preporučiti za primenu u gajilištima za suzbijanje zelene plesni. Antifungalna aktivnost dva biofungicida, na bazi Bacillus subtilis i ulja čajnog drveta, je testirana in vitro na sve Trichoderma spp. izolate. B. subtilis je bio visoko toksičan za sve testirane izolate (EC50  lt 1,3 mg/L), dok ulje čajnog drveta nije pokazalo značajnu antifungalnu aktivnost (EC50 = 11,9 – 370,8 mg/L). Efikasnost biofungicida protiv vrste T. harzianum je ocenjena u oglednom gajilištu. Biofungicidi su primenjeni zasebno ili u kombinaciji sa fungicidom, prohloraz- Mn, u proporciji 20:80%. Prohloraz-Mn se pokazao efikasnijim od oba biofungicida kao i od kombinovane primene biofungicida i fungicida. Biofungicid na bazi B. subtilis je bio efikasniji od ulja čajnog drveta u pogledu sprečavanja pojave simptoma bolesti. Takođe, B. subtilis je pokazao manji antagonistički efekat u efikasnosti protiv patogena od ulja čajnog drveta kada se primenjuju u kombinaciji sa prohloraz-Mn fungicidom.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Trichoderma species causing green mould disease of button mushroom [Agaricus bisporus (Lange) Imbach] and their sensitivity to fungicides and biofungicides
T1  - Vrste roda Trichoderma, uzročnici zelene plesni šampinjona [Agaricus bisporus (Lange) Imbach] i njihova osetljivost na fungicide i biofungicide
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5088
ER  - 

@phdthesis{
author = "Kosanović, Dejana",
year = "2015",
abstract = "Trichoderma species, the causal agents of green mould disease, induce great losses in button mushroom (Agaricus bisporus) farms. Twenty Trichoderma isolates were collected on 13 Serbian A. bisporus farms and one in Bosnia and Herzegovina during 2006 – 2010. Twelve isolates were classified into five species by standard mycological studies and ITS1/ITS4 sequence analyses, namely Trichoderma atroviride, Trichoderma koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum and Trichoderma harzianum. Eight isolates were not identified to the species level but were shown to be related to T. harzianum. The isolates of T. harzianum exhibited the highest virulence to the harvested A. bisporus pilei and T. virens and T. aggressivum f. europaeum the lowest. Fungicides are widely used to control mushroom diseases although green mould control is encumbered with difficulties. The aims of this study were therefore to research in vitro toxicity of several commercial fungicides to Trichoderma isolates originating from Serbian and Bosnia-Herzegovina farms, and to evaluate the effects of pH and light on their growth. The majority of isolates demonstrated optimal growth at pH 5.0, and the rest at pH 6.0. A few isolates also grew well at pH 7. The weakest mycelial growth was noted at pH 8.0 – 9.0. Generally, light had an inhibitory effect on the growth of tested isolates. The isolates showed the highest susceptibility to prochloraz-Mn, chlorothalonil and carbendazim (ED50 less than 1 mg/L), and were less sensitive to iprodione (ED50 ranged 0.84 – 6.72 mg/L), weakly resistant to thiophanate-methyl (ED50 = 3.75 – 24.13 mg/L), and resistant to trifloxystrobin (ED50 = 10.25 – 178.23 mg/L). Considering the toxicity of fungicides to A. bisporus, prochloraz-Mn and carbendazim showed the best selective toxicity (0.05 and 0.02 respectively), iprodione and chlorothalonil moderate (0.16), and thiophanate-methyl the lowest (1.24), while trifloxystrobin toxicity to A. bisporus was not tested because of its inefficiency against Trichoderma isolates. The best selective toxicity to both pathogen and the host showed prochloraz-Mn and carbendazim with values less than 0.1. Carbendazim was withdrawn from the market and therefore prochloraz-Mn could be recommended for mushroom cultivation in Serbia. Antifungal activity of two biofungicides based on Bacillus subtilis and tea tree oil were tested in vitro to all Trichoderma isolates. B. subtilis was highly toxic to all tested Trichoderma isolates, their ED50 values were below 1.3 mg/L. Tea tree oil did not exhibit a significant antifungal activity (ED50 = 11.9 – 370.8 mg/L). The efficacy of biofungicides was evaluated against T. harzianum in a mushroom growing room, and they were applied alone or in combination with the fungicide at a respective proportion of 20:80%. Prochloraz-Mn showed higher efficacy than both tested biofungicides or their respective mixtures. The biofungicide based on B. subtilis demonstrated greater efficacy in preventing disease symptoms than tea tree oil. B. subtilis combined with the fungicide revealed less antagonism in efficacy against pathogen than tea tree oil., Pojedine vrste roda Trichoderma su uzročnici zelene plesni šampinjona (Agaricus bisporus) i nanose velike materijalne štete u gajilištima. Od 2006. do 2010. godine prikupljeno je 20 izolata roda Trichoderma iz 13 gajilišta u Srbiji i jednog iz Bosne i Hercegovine. Dvanaest izolata je klasifikovano na osnovu standardnih mikoloških metoda i analizom ITS1/ITS4 sekvence u pet vrsta: Trichoderma atroviride, Trichoderma koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum i Trichoderma harzianum. Osam izolata nije identifikovano do nivoa vrste ali je pokazano da su filogenetski bliski sa vrstom T. harzianum. Najvišu virulentnost na ubranom šeširu A. bisporus je pokazala vrsta T. harzianum, a najnižu T. virens i T. aggressivum f. europaeum. Većina izolata je najbolje rasla na podlozi koja je imala pH = 5, potom na pH = 6, par izolata na pH = 7, dok je najslabiji rast zabeležen na pH = 8 – 9. Generalno, svetlost je imala inhibitorni efekat na rast izolata. Fungicidi su u širokoj upotrebi u kontroli bolesti šampinjona, iako je kontrola zelene plesni otežana. Stoga, jedan od ciljeva istraživanja je bio da se ispita in vitro toksičnost nekoliko dostupnih komercijalnih fungicida na prikupljene izolate roda Trichoderma. Testirani izolati su bili najosetljivi na prohloraz-Mn, hlorotalonil i karbendazim (EC50  1 mg/L), osetljivi na iprodion (EC50 = 0,84 – 6,72 mg/L), umereno rezistentni na tiofanat-metil (EC50 = 3,75 – 24,13 mg/L) i rezistentni na trifloksistrobin (EC50 = 10,25 – 178,23 mg/L). Proučavajući toksičnost fungicida na A. bisporus, najbolju selektivnu toksičnost su pokazali prohloraz-Mn (0,05) i karbendazim (0,02), iprodion i hlorotalonil umerenu (0,16), tiofanat-metil najnižu (1,24), dok toksičnost trifloksistrobina za A. bisporus nije testirana zbog nezadovoljavajuće toksičnosti za Trichoderma spp. izolate. Najbolju selektivnu toksičnost za patogenu gljivu i gljivu domaćina su pokazali prohloraz-Mn i karbendazim sa vrednostima nižim od 0,1. Pošto se karbendazim povlači sa tržišta, prohloraz-Mn je fungicid koji se može preporučiti za primenu u gajilištima za suzbijanje zelene plesni. Antifungalna aktivnost dva biofungicida, na bazi Bacillus subtilis i ulja čajnog drveta, je testirana in vitro na sve Trichoderma spp. izolate. B. subtilis je bio visoko toksičan za sve testirane izolate (EC50  lt 1,3 mg/L), dok ulje čajnog drveta nije pokazalo značajnu antifungalnu aktivnost (EC50 = 11,9 – 370,8 mg/L). Efikasnost biofungicida protiv vrste T. harzianum je ocenjena u oglednom gajilištu. Biofungicidi su primenjeni zasebno ili u kombinaciji sa fungicidom, prohloraz- Mn, u proporciji 20:80%. Prohloraz-Mn se pokazao efikasnijim od oba biofungicida kao i od kombinovane primene biofungicida i fungicida. Biofungicid na bazi B. subtilis je bio efikasniji od ulja čajnog drveta u pogledu sprečavanja pojave simptoma bolesti. Takođe, B. subtilis je pokazao manji antagonistički efekat u efikasnosti protiv patogena od ulja čajnog drveta kada se primenjuju u kombinaciji sa prohloraz-Mn fungicidom.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Trichoderma species causing green mould disease of button mushroom [Agaricus bisporus (Lange) Imbach] and their sensitivity to fungicides and biofungicides, Vrste roda Trichoderma, uzročnici zelene plesni šampinjona [Agaricus bisporus (Lange) Imbach] i njihova osetljivost na fungicide i biofungicide",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5088"
}

Kosanović, D.. (2015). Trichoderma species causing green mould disease of button mushroom [Agaricus bisporus (Lange) Imbach] and their sensitivity to fungicides and biofungicides. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_5088

Kosanović D. Trichoderma species causing green mould disease of button mushroom [Agaricus bisporus (Lange) Imbach] and their sensitivity to fungicides and biofungicides. 2015;.
https://hdl.handle.net/21.15107/rcub_nardus_5088 .

Kosanović, Dejana, "Trichoderma species causing green mould disease of button mushroom [Agaricus bisporus (Lange) Imbach] and their sensitivity to fungicides and biofungicides" (2015),
https://hdl.handle.net/21.15107/rcub_nardus_5088 .