TY  - JOUR
AU  - Vasiljević, Z. V.
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Minić, Predrag
AU  - Sovtić, A.
AU  - Đukić, S.
AU  - Jovčić, Branko
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/908
UR  - http://intor.torlakinstitut.com/handle/123456789/704
AB  - The Burkholderia cepacia complex (Bcc) organisms remain significant pathogens in patients with cystic fibrosis (CF). This study was performed to evaluate the prevalence, epidemiological characteristics, and presence of molecular markers associated with virulence and transmissibility of the Bcc strains in the National CF Centre in Belgrade, Serbia. The Bcc isolates collected during the four-year study period (2010-2013) were further examined by 16 s rRNA gene, pulsed-field gel electrophoresis of genomic DNA, multilocus sequence typing analysis, and phylogenetic analysis based on concatenated sequence of seven alleles. Fifty out of 184 patients (27.2 %) were colonized with two Bcc species, B. cenocepacia (n = 49) and B. stabilis (n = 1). Thirty-four patients (18.5 %) had chronic colonization. Typing methods revealed a high level of similarity among Bcc isolates, indicating a person-to-person transmission or acquisition from a common source. New sequence types (STs) were identified, and none of the STs with an international distribution were found. One centre-specific ST, B. cenocepacia ST856, was highly dominant and shared by 48/50 (96 %) patients colonized by Bcc. This clone was characterized by PCR positivity for both the B. cepacia epidemic strain marker and cable pilin, and showed close genetic relatedness to the epidemic strain CZ1 (ST32). These results indicate that the impact of Bcc on airway colonization in the Serbian CF population is high and virtually exclusively limited to a single clone of B. cenocepacia. The presence of a highly transmissible clone and probable patient-to-patient spread was observed.
PB  - Springer, New York
T2  - European Journal of Clinical Microbiology & Infectious Diseases
T1  - Burkholderia cepacia complex in Serbian patients with cystic fibrosis: prevalence and molecular epidemiology
EP  - 1284
IS  - 8
SP  - 1277
VL  - 35
DO  - 10.1007/s10096-016-2662-4
ER  - 

@article{
author = "Vasiljević, Z. V. and Novović, Katarina and Kojić, Milan and Minić, Predrag and Sovtić, A. and Đukić, S. and Jovčić, Branko",
year = "2016",
abstract = "The Burkholderia cepacia complex (Bcc) organisms remain significant pathogens in patients with cystic fibrosis (CF). This study was performed to evaluate the prevalence, epidemiological characteristics, and presence of molecular markers associated with virulence and transmissibility of the Bcc strains in the National CF Centre in Belgrade, Serbia. The Bcc isolates collected during the four-year study period (2010-2013) were further examined by 16 s rRNA gene, pulsed-field gel electrophoresis of genomic DNA, multilocus sequence typing analysis, and phylogenetic analysis based on concatenated sequence of seven alleles. Fifty out of 184 patients (27.2 %) were colonized with two Bcc species, B. cenocepacia (n = 49) and B. stabilis (n = 1). Thirty-four patients (18.5 %) had chronic colonization. Typing methods revealed a high level of similarity among Bcc isolates, indicating a person-to-person transmission or acquisition from a common source. New sequence types (STs) were identified, and none of the STs with an international distribution were found. One centre-specific ST, B. cenocepacia ST856, was highly dominant and shared by 48/50 (96 %) patients colonized by Bcc. This clone was characterized by PCR positivity for both the B. cepacia epidemic strain marker and cable pilin, and showed close genetic relatedness to the epidemic strain CZ1 (ST32). These results indicate that the impact of Bcc on airway colonization in the Serbian CF population is high and virtually exclusively limited to a single clone of B. cenocepacia. The presence of a highly transmissible clone and probable patient-to-patient spread was observed.",
publisher = "Springer, New York",
journal = "European Journal of Clinical Microbiology & Infectious Diseases",
title = "Burkholderia cepacia complex in Serbian patients with cystic fibrosis: prevalence and molecular epidemiology",
pages = "1284-1277",
number = "8",
volume = "35",
doi = "10.1007/s10096-016-2662-4"
}

Vasiljević, Z. V., Novović, K., Kojić, M., Minić, P., Sovtić, A., Đukić, S.,& Jovčić, B.. (2016). Burkholderia cepacia complex in Serbian patients with cystic fibrosis: prevalence and molecular epidemiology. in European Journal of Clinical Microbiology & Infectious Diseases
Springer, New York., 35(8), 1277-1284.
https://doi.org/10.1007/s10096-016-2662-4

Vasiljević ZV, Novović K, Kojić M, Minić P, Sovtić A, Đukić S, Jovčić B. Burkholderia cepacia complex in Serbian patients with cystic fibrosis: prevalence and molecular epidemiology. in European Journal of Clinical Microbiology & Infectious Diseases. 2016;35(8):1277-1284.
doi:10.1007/s10096-016-2662-4 .

Vasiljević, Z. V., Novović, Katarina, Kojić, Milan, Minić, Predrag, Sovtić, A., Đukić, S., Jovčić, Branko, "Burkholderia cepacia complex in Serbian patients with cystic fibrosis: prevalence and molecular epidemiology" in European Journal of Clinical Microbiology & Infectious Diseases, 35, no. 8 (2016):1277-1284,
https://doi.org/10.1007/s10096-016-2662-4 . .