TY  - JOUR
AU  - Kosec, Duško
AU  - Lavrnić, Dragana
AU  - Apostolski, Slobodan
AU  - Leposavić, Gordana
PY  - 2005
UR  - http://intor.torlakinstitut.com/handle/123456789/197
AB  - This study was undertaken to investigate T-cell maturation ill hyperplastic thymi of patients suffering from myasthenia gravis (MG). For this purpose, the expression of the major differentiational molecules (CD4, CD8, and CD3/TCRalphass) and that of the regulator. v and activation molecules oil thymocytes from MG patients and control subjects were estimated by flow cytometric analysis. In the MG patients the increase in relative proportion of immature (CD4-8- TCRalphass-) and the most mature (CD4+8- TCRalphass(high) and CD4-8- TCRhigh encompassing immunoregulatory NKT) thymocytes followed by a decrease in that of CD4+8+CD3 /TCRalphass- cells was found. Furthermore, in these patients the relative proportion of CD4+HLA-DR+ and CD4+71+ cells was increased, whereas that of CD4+25+ cells was slightly, but significantly, decreased (reflecting, most likely, decreased contribution of T reg cells bearing this phenotype). Moreover. in MG thymi the percentage of CD45RA+ cells was reduced indicating changes in the selection processes. In keeping with this finding the reduced thymocyte apoptotic index and percentage of cells bearing apoptosing (CD4-8- TCRalphass(low)) phenotype were detected. In conclusion, the study demonstrates substantial changes in intrathymic differentiation of T cells in hyperplastic MG thymi and suggests alterations in selection events providing an increased escape of potentially? autoreactive T-cell clones, on one side, and all altered maturation and/or selection of immunoregulatory cells (NKT and CD4+8-25+ T reg cells) keeping these cell clones under control, on the other side.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Changes in thymopoiesis in myasthenia gravis
EP  - 243
IS  - 2
SP  - 223
VL  - 115
DO  - 10.1080/00207450590519472
ER  - 

@article{
author = "Kosec, Duško and Lavrnić, Dragana and Apostolski, Slobodan and Leposavić, Gordana",
year = "2005",
abstract = "This study was undertaken to investigate T-cell maturation ill hyperplastic thymi of patients suffering from myasthenia gravis (MG). For this purpose, the expression of the major differentiational molecules (CD4, CD8, and CD3/TCRalphass) and that of the regulator. v and activation molecules oil thymocytes from MG patients and control subjects were estimated by flow cytometric analysis. In the MG patients the increase in relative proportion of immature (CD4-8- TCRalphass-) and the most mature (CD4+8- TCRalphass(high) and CD4-8- TCRhigh encompassing immunoregulatory NKT) thymocytes followed by a decrease in that of CD4+8+CD3 /TCRalphass- cells was found. Furthermore, in these patients the relative proportion of CD4+HLA-DR+ and CD4+71+ cells was increased, whereas that of CD4+25+ cells was slightly, but significantly, decreased (reflecting, most likely, decreased contribution of T reg cells bearing this phenotype). Moreover. in MG thymi the percentage of CD45RA+ cells was reduced indicating changes in the selection processes. In keeping with this finding the reduced thymocyte apoptotic index and percentage of cells bearing apoptosing (CD4-8- TCRalphass(low)) phenotype were detected. In conclusion, the study demonstrates substantial changes in intrathymic differentiation of T cells in hyperplastic MG thymi and suggests alterations in selection events providing an increased escape of potentially? autoreactive T-cell clones, on one side, and all altered maturation and/or selection of immunoregulatory cells (NKT and CD4+8-25+ T reg cells) keeping these cell clones under control, on the other side.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Changes in thymopoiesis in myasthenia gravis",
pages = "243-223",
number = "2",
volume = "115",
doi = "10.1080/00207450590519472"
}

Kosec, D., Lavrnić, D., Apostolski, S.,& Leposavić, G.. (2005). Changes in thymopoiesis in myasthenia gravis. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 115(2), 223-243.
https://doi.org/10.1080/00207450590519472

Kosec D, Lavrnić D, Apostolski S, Leposavić G. Changes in thymopoiesis in myasthenia gravis. in International Journal of Neuroscience. 2005;115(2):223-243.
doi:10.1080/00207450590519472 .

Kosec, Duško, Lavrnić, Dragana, Apostolski, Slobodan, Leposavić, Gordana, "Changes in thymopoiesis in myasthenia gravis" in International Journal of Neuroscience, 115, no. 2 (2005):223-243,
https://doi.org/10.1080/00207450590519472 . .

TY  - JOUR
AU  - Dimitrijević, Ljiljana
AU  - Radulović, M.I.
AU  - Ćirić, B.P.
AU  - Petrićević, Marijana
AU  - Inić, Aleksandra
AU  - Nikolić, D.
AU  - Apostolski, Slobodan
PY  - 1999
UR  - http://intor.torlakinstitut.com/handle/123456789/111
AB  - In this study we tried to elucidate further the crossreactivity pattern and binding characteristics of human monoclonal IgM DJ which is an anti-DNA antibody and possesses Y7 natural idiotope. Isolated IgM DJ and its enzymatically obtained fragments Fab' and (Fab')2 were tested for binding to more than 26 antigens and nine bacteria in indirect ELISA. Inhibition of binding studies and examination of the stability of antigen-antibody complexes were also done in ELISA assay. IgM DJ bound to single stranded DNA and human lactic acid bacteria, such as L. acidophyllus, B. bifidum and L. plantarum. This binding was shown to be mediated through IgM DJ Fab' fragment. High avidity and low affinity of interactions was estimated from the binding curves of Fab', (Fab')2 fragments and whole IgM. The common epitopic motif on both antigens were negatively charged phosphodiester moieties. Complexes formed with ssDNA and B. bifidum were resistant to washing with high salt. This suggested that electrostatic attraction was not a strong component of the binding. A novel pattern of natural autoantibody reactivity in a human system related to cross-reactivity with DNA and LAB is described. Possible involvement of LAB in induction of natural anti-DNA antibodies is discussed.
PB  - IOS Press
T2  - Human Antibodies
T1  - Human monoclonal IgM DJ binds to ssDNA and human commensal bacteria
EP  - 45
IS  - 1
SP  - 37
VL  - 9
DO  - 10.3233/hab-1999-9104
ER  - 

@article{
author = "Dimitrijević, Ljiljana and Radulović, M.I. and Ćirić, B.P. and Petrićević, Marijana and Inić, Aleksandra and Nikolić, D. and Apostolski, Slobodan",
year = "1999",
abstract = "In this study we tried to elucidate further the crossreactivity pattern and binding characteristics of human monoclonal IgM DJ which is an anti-DNA antibody and possesses Y7 natural idiotope. Isolated IgM DJ and its enzymatically obtained fragments Fab' and (Fab')2 were tested for binding to more than 26 antigens and nine bacteria in indirect ELISA. Inhibition of binding studies and examination of the stability of antigen-antibody complexes were also done in ELISA assay. IgM DJ bound to single stranded DNA and human lactic acid bacteria, such as L. acidophyllus, B. bifidum and L. plantarum. This binding was shown to be mediated through IgM DJ Fab' fragment. High avidity and low affinity of interactions was estimated from the binding curves of Fab', (Fab')2 fragments and whole IgM. The common epitopic motif on both antigens were negatively charged phosphodiester moieties. Complexes formed with ssDNA and B. bifidum were resistant to washing with high salt. This suggested that electrostatic attraction was not a strong component of the binding. A novel pattern of natural autoantibody reactivity in a human system related to cross-reactivity with DNA and LAB is described. Possible involvement of LAB in induction of natural anti-DNA antibodies is discussed.",
publisher = "IOS Press",
journal = "Human Antibodies",
title = "Human monoclonal IgM DJ binds to ssDNA and human commensal bacteria",
pages = "45-37",
number = "1",
volume = "9",
doi = "10.3233/hab-1999-9104"
}

Dimitrijević, L., Radulović, M.I., Ćirić, B.P., Petrićević, M., Inić, A., Nikolić, D.,& Apostolski, S.. (1999). Human monoclonal IgM DJ binds to ssDNA and human commensal bacteria. in Human Antibodies
IOS Press., 9(1), 37-45.
https://doi.org/10.3233/hab-1999-9104

Dimitrijević L, Radulović M, Ćirić B, Petrićević M, Inić A, Nikolić D, Apostolski S. Human monoclonal IgM DJ binds to ssDNA and human commensal bacteria. in Human Antibodies. 1999;9(1):37-45.
doi:10.3233/hab-1999-9104 .

Dimitrijević, Ljiljana, Radulović, M.I., Ćirić, B.P., Petrićević, Marijana, Inić, Aleksandra, Nikolić, D., Apostolski, Slobodan, "Human monoclonal IgM DJ binds to ssDNA and human commensal bacteria" in Human Antibodies, 9, no. 1 (1999):37-45,
https://doi.org/10.3233/hab-1999-9104 . .

TY  - JOUR
AU  - Dimitrijević, Ljiljana
AU  - Radulović, M.
AU  - Cirić, B.
AU  - Inić, Aleksandra
AU  - Apostolski, Slobodan
PY  - 1996
UR  - http://intor.torlakinstitut.com/handle/123456789/69
AB  - We have already characterized Y7 idiotype as natural idiotype by showing its expression on IgM molecules derived form cord sera. The aim of this study was to investigate the binding properties of two human monoclonal IgM antibodies isolated from the sera of patients with Waldenstrom macroglobulinaemia, which express Y7 idiotype. In order to estimate the binding repertoire of these two IgM molecules (IgM DJ and IgM RD) we tested the isolated IgM molecules and their F(ab)2 and Fab fragments over the panel of 23 different protein and non-protein antigens, nine different bacterial strains and cell membranes from neuronal and epithelial tissues. These tests were performed by means of direct and competitive ELISA, immunoblot and immunofluorescence assays. Both antibodies reacted with ssDNA, oligonucleotide fragments, and lactobacteria. IgM DJ showed reactivity with myelin associated glycoprotein (MAG) and blood vessel endothelial cells. Binding for DNA oligonucleotide fragments and lactobacteria was interrupted up to 70% with 0.5 M NaCl, which indicated that these interactions were of low affinity. Avidity dependent binding was also evidenced by use of F(ab)2 fragments. Determined characteristics such as polyreactivity which included autoantigens, low affinity and avidity dependent binding, qualified these two monoclonal IgM as natural autoantibodies. Expression of natural idiotype Y7 on two immunoglobulins M,which possessed natural autoantibody properties, indicated the connection of natural autoantibody specificity and the natural idiotype expression and suggested the involvement of network interactions in selection of malignant B cell clones.
PB  - Forefront Publishing Group
T2  - Human Antibodies and Hybridomas
T1  - Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype
IS  - 2
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_intor_69
ER  - 

@article{
author = "Dimitrijević, Ljiljana and Radulović, M. and Cirić, B. and Inić, Aleksandra and Apostolski, Slobodan",
year = "1996",
abstract = "We have already characterized Y7 idiotype as natural idiotype by showing its expression on IgM molecules derived form cord sera. The aim of this study was to investigate the binding properties of two human monoclonal IgM antibodies isolated from the sera of patients with Waldenstrom macroglobulinaemia, which express Y7 idiotype. In order to estimate the binding repertoire of these two IgM molecules (IgM DJ and IgM RD) we tested the isolated IgM molecules and their F(ab)2 and Fab fragments over the panel of 23 different protein and non-protein antigens, nine different bacterial strains and cell membranes from neuronal and epithelial tissues. These tests were performed by means of direct and competitive ELISA, immunoblot and immunofluorescence assays. Both antibodies reacted with ssDNA, oligonucleotide fragments, and lactobacteria. IgM DJ showed reactivity with myelin associated glycoprotein (MAG) and blood vessel endothelial cells. Binding for DNA oligonucleotide fragments and lactobacteria was interrupted up to 70% with 0.5 M NaCl, which indicated that these interactions were of low affinity. Avidity dependent binding was also evidenced by use of F(ab)2 fragments. Determined characteristics such as polyreactivity which included autoantigens, low affinity and avidity dependent binding, qualified these two monoclonal IgM as natural autoantibodies. Expression of natural idiotype Y7 on two immunoglobulins M,which possessed natural autoantibody properties, indicated the connection of natural autoantibody specificity and the natural idiotype expression and suggested the involvement of network interactions in selection of malignant B cell clones.",
publisher = "Forefront Publishing Group",
journal = "Human Antibodies and Hybridomas",
title = "Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype",
number = "2",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_intor_69"
}

Dimitrijević, L., Radulović, M., Cirić, B., Inić, A.,& Apostolski, S.. (1996). Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype. in Human Antibodies and Hybridomas
Forefront Publishing Group., 7(2).
https://hdl.handle.net/21.15107/rcub_intor_69

Dimitrijević L, Radulović M, Cirić B, Inić A, Apostolski S. Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype. in Human Antibodies and Hybridomas. 1996;7(2).
https://hdl.handle.net/21.15107/rcub_intor_69 .

Dimitrijević, Ljiljana, Radulović, M., Cirić, B., Inić, Aleksandra, Apostolski, Slobodan, "Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype" in Human Antibodies and Hybridomas, 7, no. 2 (1996),
https://hdl.handle.net/21.15107/rcub_intor_69 .

TY  - CONF
AU  - Popesković, L.
AU  - Arsenović, Nevena
AU  - Apostolski, Slobodan
AU  - Isaković, Katarina
PY  - 1990
UR  - http://intor.torlakinstitut.com/handle/123456789/43
PB  - Hrvatsko prirodoslovno društvo, Zagreb
C3  - Periodicum Biologorum
T1  - Thymic microenvironment in myasthenia gravis: Cellular analysis of nonlymphoid cells grown in tissue culture
EP  - 101
IS  - 1
SP  - 100
VL  - 92
UR  - https://hdl.handle.net/21.15107/rcub_intor_43
ER  - 

@conference{
author = "Popesković, L. and Arsenović, Nevena and Apostolski, Slobodan and Isaković, Katarina",
year = "1990",
publisher = "Hrvatsko prirodoslovno društvo, Zagreb",
journal = "Periodicum Biologorum",
title = "Thymic microenvironment in myasthenia gravis: Cellular analysis of nonlymphoid cells grown in tissue culture",
pages = "101-100",
number = "1",
volume = "92",
url = "https://hdl.handle.net/21.15107/rcub_intor_43"
}

Popesković, L., Arsenović, N., Apostolski, S.,& Isaković, K.. (1990). Thymic microenvironment in myasthenia gravis: Cellular analysis of nonlymphoid cells grown in tissue culture. in Periodicum Biologorum
Hrvatsko prirodoslovno društvo, Zagreb., 92(1), 100-101.
https://hdl.handle.net/21.15107/rcub_intor_43

Popesković L, Arsenović N, Apostolski S, Isaković K. Thymic microenvironment in myasthenia gravis: Cellular analysis of nonlymphoid cells grown in tissue culture. in Periodicum Biologorum. 1990;92(1):100-101.
https://hdl.handle.net/21.15107/rcub_intor_43 .

Popesković, L., Arsenović, Nevena, Apostolski, Slobodan, Isaković, Katarina, "Thymic microenvironment in myasthenia gravis: Cellular analysis of nonlymphoid cells grown in tissue culture" in Periodicum Biologorum, 92, no. 1 (1990):100-101,
https://hdl.handle.net/21.15107/rcub_intor_43 .

TY  - CONF
AU  - Damjanović, M.
AU  - Vidaković, A.
AU  - Lazić-Lavrnić, D.
AU  - Apostolski, Slobodan
AU  - Gospavić, J.
AU  - Isaković, Katarina
PY  - 1990
UR  - http://intor.torlakinstitut.com/handle/123456789/36
PB  - Hrvatsko prirodoslovno društvo, Zagreb
C3  - Periodicum Biologorum
T1  - Effect of acetylcholine on human lymphocyte activation
EP  - 71
IS  - 1
SP  - 70
VL  - 92
UR  - https://hdl.handle.net/21.15107/rcub_intor_36
ER  - 

@conference{
author = "Damjanović, M. and Vidaković, A. and Lazić-Lavrnić, D. and Apostolski, Slobodan and Gospavić, J. and Isaković, Katarina",
year = "1990",
publisher = "Hrvatsko prirodoslovno društvo, Zagreb",
journal = "Periodicum Biologorum",
title = "Effect of acetylcholine on human lymphocyte activation",
pages = "71-70",
number = "1",
volume = "92",
url = "https://hdl.handle.net/21.15107/rcub_intor_36"
}

Damjanović, M., Vidaković, A., Lazić-Lavrnić, D., Apostolski, S., Gospavić, J.,& Isaković, K.. (1990). Effect of acetylcholine on human lymphocyte activation. in Periodicum Biologorum
Hrvatsko prirodoslovno društvo, Zagreb., 92(1), 70-71.
https://hdl.handle.net/21.15107/rcub_intor_36

Damjanović M, Vidaković A, Lazić-Lavrnić D, Apostolski S, Gospavić J, Isaković K. Effect of acetylcholine on human lymphocyte activation. in Periodicum Biologorum. 1990;92(1):70-71.
https://hdl.handle.net/21.15107/rcub_intor_36 .

Damjanović, M., Vidaković, A., Lazić-Lavrnić, D., Apostolski, Slobodan, Gospavić, J., Isaković, Katarina, "Effect of acetylcholine on human lymphocyte activation" in Periodicum Biologorum, 92, no. 1 (1990):70-71,
https://hdl.handle.net/21.15107/rcub_intor_36 .