@conference{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Vujnović, Ivana and Petrović, Raisa and Dimitrijević, Mirjana and Leposavić, Gordana",
year = "2015",
abstract = "The present study was designed to examine influence of aging on
macrophage proinflammatory/anti-inflammatory capacity in rat model of
thioglycollate-induced peritonitis. Peritoneal macrophages were isolated
from young (3-months-old) and aged (18-months-old) Dark Agouti (DA)
and Albino Oxford (AO) rats seven days post-injection of thioglycollate
medium. Freshly isolated peritoneal exudate cells were examined for the
expression of CD163, CCR7, CD14 and TLR4, whereas cytokine
production (TNF-α, IL-6 and IL-10) and arginine metabolism end-products
(NO and urea) were assayed in vitro under basal conditions and following
stimulation with LPS. In DA rat inflammatory peritoneal exudate, aging
diminished the frequency of cells with a “resolving macrophage”
CD14+CD163+ phenotype. However, in AO rats, which exhibited stable
frequency of CD14+CD163+ cells in inflammatory peritoneal exudate with
aging, the proportion of CCR7-bearing peritoneal cells, presumably
immigrating inflammatory monocytes, was diminished in aged animals.
Under basal culture conditions, macrophages from aged rats of both strains
released less amount of TNF-α, IL-6 and IL-10, but produced more urea
than cells from young strain-matched rats. However, these changes were
more pronounced in peritoneal macrophages from AO rats. Additionally,
age-related decrease in the frequency of TLR4-expressing cells was
observed among fresh peritoneal exudate cells from AO rats. Upon LPS
stimulation, the production of prototypic inflammatory cytokines (TNF-α
and IL-6) was diminished in macrophages from aged AO rats, whereas
aging had the opposite effect on their production in DA rat macrophages.
Moreover, aging increased NO production in LPS-stimulated macrophages
from DA rats, whereas urea production was enhanced in macrophages from
both strains, but this increase was strikingly more pronounced in
macrophages from AO rats. Collectively, results suggest that aging affects
inflammatory peritoneal exudate cellular composition and macrophage
proinflamatory/immunomodulatory capacity in a strain- specific manner",
publisher = "Immunological society of Serbia, University of Kragujevac, Faculty of medical sciences",
journal = "3rd Belgrade EFIS symposium on immunoregulation. Book of abstracts: Immunity, Infection, Autoimmunity and Aging. Hotel Izvor, Arandjelovac Spa (Belgrade) 24-27 May 2015",
title = "Aging affects rat inflammatory peritoneal exudate composition and macrophage inflammatory mediator production in a strain-dependent manner",
pages = "58-58",
url = "https://hdl.handle.net/21.15107/rcub_intor_666"
}