TY  - JOUR
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Arsenović-Ranin, Nevena
AU  - Blagojević, Veljko
AU  - Dimitrijević, Mirjana
AU  - Vidić-Danković, Biljana
AU  - Vujić, Vesna
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/496
AB  - The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression
EP  - 1101
IS  - 3
SP  - 1087
VL  - 40
DO  - 10.1007/s10753-017-0551-3
ER  - 

@article{
author = "Ćuruvija, Ivana and Stanojević, Stanislava and Arsenović-Ranin, Nevena and Blagojević, Veljko and Dimitrijević, Mirjana and Vidić-Danković, Biljana and Vujić, Vesna",
year = "2017",
abstract = "The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression",
pages = "1101-1087",
number = "3",
volume = "40",
doi = "10.1007/s10753-017-0551-3"
}

Ćuruvija, I., Stanojević, S., Arsenović-Ranin, N., Blagojević, V., Dimitrijević, M., Vidić-Danković, B.,& Vujić, V.. (2017). Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation
Springer/Plenum Publishers, New York., 40(3), 1087-1101.
https://doi.org/10.1007/s10753-017-0551-3

Ćuruvija I, Stanojević S, Arsenović-Ranin N, Blagojević V, Dimitrijević M, Vidić-Danković B, Vujić V. Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation. 2017;40(3):1087-1101.
doi:10.1007/s10753-017-0551-3 .

Ćuruvija, Ivana, Stanojević, Stanislava, Arsenović-Ranin, Nevena, Blagojević, Veljko, Dimitrijević, Mirjana, Vidić-Danković, Biljana, Vujić, Vesna, "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression" in Inflammation, 40, no. 3 (2017):1087-1101,
https://doi.org/10.1007/s10753-017-0551-3 . .

TY  - JOUR
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Perišić, Milica
AU  - Pilipović, Ivan
AU  - Vidić-Danković, Biljana
AU  - Leposavić, Gordana
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/333
AB  - We tested the hypothesis that neonatal androgenization affects the efficacy of beta-adrenoceptor (beta-AR)-mediated fine tuning of thymopoiesis in adult female rats by modulating the thymic noradrenaline (NA) level and/or beta-AR expression. In adult rats administered with 1000 mu g testosterone enanthate at postnatal day 2 a higher density of catecholamine (CA)-synthesizing thymic cells, including thymocytes, and a rise in their CA content was found. In addition, in these animals increased thymic noradrenergic nerve fiber fluorescence intensity, reflecting their increased CA content, was detected. These changes were followed by an increase in thymic NA concentration. The rise in thymic NA content in thymic nerve fibers and cells was associated with changes in the expression of mRNA for enzymes controling pivotal steps in NA biosynthesis (tyrosine hydroxylase, dopamine-beta-hydroxylase) and inactivation (monoamine oxidase). In contrast, the thymic level of beta(2)-AR mRNA on a per cell basis and the receptor surface density on thymocytes was reduced in testosterone-treated (TT) rats. As a consequence, 14-day-long treatment with propranolol, a beta-AR blocker, was ineffective in modulating T-cell differentiation/maturation in TT rats. In conclusion, the study indicates the importance of the neonatal sex steroid milieu for shaping the immunomodulatory capacity of the thymic NA/beta-AR signaling system in adult rats. (C) 2011 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Neonatal androgenization affects the efficiency of beta-adrenoceptor-mediated modulation of thymopoiesis
EP  - 79
IS  - 1-2
SP  - 68
VL  - 239
DO  - 10.1016/j.jneuroim.2011.08.020
ER  - 

@article{
author = "Radojević, Katarina and Kosec, Duško and Perišić, Milica and Pilipović, Ivan and Vidić-Danković, Biljana and Leposavić, Gordana",
year = "2011",
abstract = "We tested the hypothesis that neonatal androgenization affects the efficacy of beta-adrenoceptor (beta-AR)-mediated fine tuning of thymopoiesis in adult female rats by modulating the thymic noradrenaline (NA) level and/or beta-AR expression. In adult rats administered with 1000 mu g testosterone enanthate at postnatal day 2 a higher density of catecholamine (CA)-synthesizing thymic cells, including thymocytes, and a rise in their CA content was found. In addition, in these animals increased thymic noradrenergic nerve fiber fluorescence intensity, reflecting their increased CA content, was detected. These changes were followed by an increase in thymic NA concentration. The rise in thymic NA content in thymic nerve fibers and cells was associated with changes in the expression of mRNA for enzymes controling pivotal steps in NA biosynthesis (tyrosine hydroxylase, dopamine-beta-hydroxylase) and inactivation (monoamine oxidase). In contrast, the thymic level of beta(2)-AR mRNA on a per cell basis and the receptor surface density on thymocytes was reduced in testosterone-treated (TT) rats. As a consequence, 14-day-long treatment with propranolol, a beta-AR blocker, was ineffective in modulating T-cell differentiation/maturation in TT rats. In conclusion, the study indicates the importance of the neonatal sex steroid milieu for shaping the immunomodulatory capacity of the thymic NA/beta-AR signaling system in adult rats. (C) 2011 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Neonatal androgenization affects the efficiency of beta-adrenoceptor-mediated modulation of thymopoiesis",
pages = "79-68",
number = "1-2",
volume = "239",
doi = "10.1016/j.jneuroim.2011.08.020"
}

Radojević, K., Kosec, D., Perišić, M., Pilipović, I., Vidić-Danković, B.,& Leposavić, G.. (2011). Neonatal androgenization affects the efficiency of beta-adrenoceptor-mediated modulation of thymopoiesis. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 239(1-2), 68-79.
https://doi.org/10.1016/j.jneuroim.2011.08.020

Radojević K, Kosec D, Perišić M, Pilipović I, Vidić-Danković B, Leposavić G. Neonatal androgenization affects the efficiency of beta-adrenoceptor-mediated modulation of thymopoiesis. in Journal of Neuroimmunology. 2011;239(1-2):68-79.
doi:10.1016/j.jneuroim.2011.08.020 .

Radojević, Katarina, Kosec, Duško, Perišić, Milica, Pilipović, Ivan, Vidić-Danković, Biljana, Leposavić, Gordana, "Neonatal androgenization affects the efficiency of beta-adrenoceptor-mediated modulation of thymopoiesis" in Journal of Neuroimmunology, 239, no. 1-2 (2011):68-79,
https://doi.org/10.1016/j.jneuroim.2011.08.020 . .

TY  - JOUR
AU  - Pešić, Vesna
AU  - Kosec, Duško
AU  - Radojević, Katarina
AU  - Pilipović, Ivan
AU  - Perišić, Milica
AU  - Vidić-Danković, Biljana
AU  - Leposavić, Gordana
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/272
AB  - The study was undertaken to explore: i) the presence of alpha(1)-adrenoceptors (AR) on thymic lymphoid and non-lymphoid cells and ii) their putative role in T-cell development. The expression of alpha(1)-AR on thymic cells was assessed using both immunohistochemistry and flow cytometric analyses, while their putative role in thymopoiesis was estimated by analyses of thymocyte proliferation and apoptosis, and major thymocyte subset distribution in adult rats subjected to 14-day-long treatment with the alpha(1)-AR blocker urapidil. The presence of alpha(1)-AR was demonstrated on both thymocytes (mainly less mature CD3(-) and CD3(low) cells) and thymic non-lymphoid cells (thymic epithelial cells and CD68-positive cells). Chronic treatment with urapidil increased the thymic weight and thymocyte number. The increase in thymocyte number might, at least partly, be related to an enhanced thymocyte proliferation. In addition, an altered thymocyte subset distribution was observed in these rats. The increase in the percentage of CD4+CD8+ double positive (DP) TCR alpha beta(-) thymocytes was accompanied by the reduction in that of CD4+CD8+ (DP) TCR alpha beta(low) cells, and divergent changes in the percentage of the most mature single positive (SP) TCR alpha beta(high) high thymocytes. In urapidil-administered rats the percentage of CD4+CD8-SP TCR alpha beta(high) thymocytes was increased, while that of the CD4-CD8+ TCR alpha beta(high) was reduced. compared with controls. In addition, proportions of CD4+CD25+ RT6.1- and CD161+TCR alpha beta+ regulatory cells were increased. Collectively, the results indicate that alpha(1)-AR are involved in complex network of neuro-thymic and intrathymic communications that provide fine tuning of both conventional effector and regulatory T-cell development. (c) 2009 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Expression of alpha(1)-adrenoceptors on thymic cells and their role in fine tuning of thymopoiesis
EP  - 66
IS  - 1-2
SP  - 55
VL  - 214
DO  - 10.1016/j.jneuroim.2009.06.018
ER  - 

@article{
author = "Pešić, Vesna and Kosec, Duško and Radojević, Katarina and Pilipović, Ivan and Perišić, Milica and Vidić-Danković, Biljana and Leposavić, Gordana",
year = "2009",
abstract = "The study was undertaken to explore: i) the presence of alpha(1)-adrenoceptors (AR) on thymic lymphoid and non-lymphoid cells and ii) their putative role in T-cell development. The expression of alpha(1)-AR on thymic cells was assessed using both immunohistochemistry and flow cytometric analyses, while their putative role in thymopoiesis was estimated by analyses of thymocyte proliferation and apoptosis, and major thymocyte subset distribution in adult rats subjected to 14-day-long treatment with the alpha(1)-AR blocker urapidil. The presence of alpha(1)-AR was demonstrated on both thymocytes (mainly less mature CD3(-) and CD3(low) cells) and thymic non-lymphoid cells (thymic epithelial cells and CD68-positive cells). Chronic treatment with urapidil increased the thymic weight and thymocyte number. The increase in thymocyte number might, at least partly, be related to an enhanced thymocyte proliferation. In addition, an altered thymocyte subset distribution was observed in these rats. The increase in the percentage of CD4+CD8+ double positive (DP) TCR alpha beta(-) thymocytes was accompanied by the reduction in that of CD4+CD8+ (DP) TCR alpha beta(low) cells, and divergent changes in the percentage of the most mature single positive (SP) TCR alpha beta(high) high thymocytes. In urapidil-administered rats the percentage of CD4+CD8-SP TCR alpha beta(high) thymocytes was increased, while that of the CD4-CD8+ TCR alpha beta(high) was reduced. compared with controls. In addition, proportions of CD4+CD25+ RT6.1- and CD161+TCR alpha beta+ regulatory cells were increased. Collectively, the results indicate that alpha(1)-AR are involved in complex network of neuro-thymic and intrathymic communications that provide fine tuning of both conventional effector and regulatory T-cell development. (c) 2009 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Expression of alpha(1)-adrenoceptors on thymic cells and their role in fine tuning of thymopoiesis",
pages = "66-55",
number = "1-2",
volume = "214",
doi = "10.1016/j.jneuroim.2009.06.018"
}

Pešić, V., Kosec, D., Radojević, K., Pilipović, I., Perišić, M., Vidić-Danković, B.,& Leposavić, G.. (2009). Expression of alpha(1)-adrenoceptors on thymic cells and their role in fine tuning of thymopoiesis. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 214(1-2), 55-66.
https://doi.org/10.1016/j.jneuroim.2009.06.018

Pešić V, Kosec D, Radojević K, Pilipović I, Perišić M, Vidić-Danković B, Leposavić G. Expression of alpha(1)-adrenoceptors on thymic cells and their role in fine tuning of thymopoiesis. in Journal of Neuroimmunology. 2009;214(1-2):55-66.
doi:10.1016/j.jneuroim.2009.06.018 .

Pešić, Vesna, Kosec, Duško, Radojević, Katarina, Pilipović, Ivan, Perišić, Milica, Vidić-Danković, Biljana, Leposavić, Gordana, "Expression of alpha(1)-adrenoceptors on thymic cells and their role in fine tuning of thymopoiesis" in Journal of Neuroimmunology, 214, no. 1-2 (2009):55-66,
https://doi.org/10.1016/j.jneuroim.2009.06.018 . .

TY  - JOUR
AU  - Pilipović, Ivan
AU  - Vidić-Danković, Biljana
AU  - Perišić, Milica
AU  - Radojević, Katarina
AU  - Colić, Miodrag
AU  - Todorović, Vera
AU  - Leposavić, Gordana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/250
AB  - The study was undertaken to explore whether there were: i) apart from neural and circulatory, some other sources of catecholamines (CAs) in rat thymus and ii) gender-specific differences in thymic CA levels, and if so to elucidate the role of sex steroids in this phenomenon. Tyrosine hydroxylase (TH) immunoreactivity was found in thymocytes and thymic epithelial cells (some of which showed morphological features of nurse cells). The density of CA-synthesizing cells was greater in male than in female rats. Noradrenaline (NA), but not dopamine (DA), was detected in thymocytes. NA and DA levels in thymi, and the NA level in thymocytes, were higher in male rats. To explore the Putative role of sex steroids in this dichotomy in the thymi of adult rats gonadectomized (Gx) or sham-Gx at the age of 30 days the density of TH+ cells and CA levels were measured. Gonadectomy abolished sexual dimorphism in the density of thymic TH+ cells (diminishing their density in male rats) and thymic CA levels (the NA levels were reduced in rats of both sexes and also the DA level in male rats). Therefore, it can be assumed that testicular and ovarian hormones control thymic NA and DA levels via different mechanisms. Moreover, in Gx rats, despite the decrease in the overall thymic NA level, an increase in the thymocyte NA level was found indicating that gonadal hormones exert differential effects on the NA level in distinct thymic cellular compartments. (C) 2007 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones
EP  - 20
IS  - 1-2
SP  - 7
VL  - 195
DO  - 10.1016/j.jneuroim.2007.12.006
ER  - 

@article{
author = "Pilipović, Ivan and Vidić-Danković, Biljana and Perišić, Milica and Radojević, Katarina and Colić, Miodrag and Todorović, Vera and Leposavić, Gordana",
year = "2008",
abstract = "The study was undertaken to explore whether there were: i) apart from neural and circulatory, some other sources of catecholamines (CAs) in rat thymus and ii) gender-specific differences in thymic CA levels, and if so to elucidate the role of sex steroids in this phenomenon. Tyrosine hydroxylase (TH) immunoreactivity was found in thymocytes and thymic epithelial cells (some of which showed morphological features of nurse cells). The density of CA-synthesizing cells was greater in male than in female rats. Noradrenaline (NA), but not dopamine (DA), was detected in thymocytes. NA and DA levels in thymi, and the NA level in thymocytes, were higher in male rats. To explore the Putative role of sex steroids in this dichotomy in the thymi of adult rats gonadectomized (Gx) or sham-Gx at the age of 30 days the density of TH+ cells and CA levels were measured. Gonadectomy abolished sexual dimorphism in the density of thymic TH+ cells (diminishing their density in male rats) and thymic CA levels (the NA levels were reduced in rats of both sexes and also the DA level in male rats). Therefore, it can be assumed that testicular and ovarian hormones control thymic NA and DA levels via different mechanisms. Moreover, in Gx rats, despite the decrease in the overall thymic NA level, an increase in the thymocyte NA level was found indicating that gonadal hormones exert differential effects on the NA level in distinct thymic cellular compartments. (C) 2007 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones",
pages = "20-7",
number = "1-2",
volume = "195",
doi = "10.1016/j.jneuroim.2007.12.006"
}

Pilipović, I., Vidić-Danković, B., Perišić, M., Radojević, K., Colić, M., Todorović, V.,& Leposavić, G.. (2008). Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 195(1-2), 7-20.
https://doi.org/10.1016/j.jneuroim.2007.12.006

Pilipović I, Vidić-Danković B, Perišić M, Radojević K, Colić M, Todorović V, Leposavić G. Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones. in Journal of Neuroimmunology. 2008;195(1-2):7-20.
doi:10.1016/j.jneuroim.2007.12.006 .

Pilipović, Ivan, Vidić-Danković, Biljana, Perišić, Milica, Radojević, Katarina, Colić, Miodrag, Todorović, Vera, Leposavić, Gordana, "Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones" in Journal of Neuroimmunology, 195, no. 1-2 (2008):7-20,
https://doi.org/10.1016/j.jneuroim.2007.12.006 . .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Radojević, Katarina
AU  - Vidić-Danković, Biljana
AU  - Kosec, Duško
AU  - Pilipović, Ivan
AU  - Perišić, Milica
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/226
AB  - The interactions among the nervous, endocrine and immune system were studied by examining: i) thymic and thymocyte catecholamine levels in adult rats castrated (Cx) at postnatal day 3 and ii) effects of 14-day-long propranolol (P) treatment on main thymocyte differentiational molecule expression in adult non-Cx and Cx rat. The results demonstrated that castration in early postnatal period lowers levels of both neurally- and thymocyte-derived noradrenaline in adult rats, and thereby diminishes beta-adrenoceptor-mediated fine tuning of the T-cell differentiation/maturation. In non-Cx rats P affected TCR alpha beta-dependent stages of thymocyte differentiation/maturation decreasing frequency of CD4+8+ double positive (DP) TCR alpha beta(low) low cells entering selection processes and increasing relative number of positively selected DP TCR alpha beta(high) (most likely due to an increased thymocyte surface density of Thy-1 that is involved in negative control of TCR alpha beta-mediated signaling/selection thresholds) and the most mature CD4+8- TCR alpha beta(high) cells (including CD4+25+ regulatory cells). However, in Cx rats P failed to produce any significant changes in thymocyte subset composition. (c) 2006 Published by Elsevier B.V.
PB  - Elsevier, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Early postnatal castration affects thymic and thymocyte noradrenaline levels and beta-adrenoceptor-mediated influence on the thymopoiesis in adult rats
EP  - 115
IS  - 1-2
SP  - 100
VL  - 182
DO  - 10.1016/j.jneuroim.2006.10.004
ER  - 

@article{
author = "Leposavić, Gordana and Radojević, Katarina and Vidić-Danković, Biljana and Kosec, Duško and Pilipović, Ivan and Perišić, Milica",
year = "2007",
abstract = "The interactions among the nervous, endocrine and immune system were studied by examining: i) thymic and thymocyte catecholamine levels in adult rats castrated (Cx) at postnatal day 3 and ii) effects of 14-day-long propranolol (P) treatment on main thymocyte differentiational molecule expression in adult non-Cx and Cx rat. The results demonstrated that castration in early postnatal period lowers levels of both neurally- and thymocyte-derived noradrenaline in adult rats, and thereby diminishes beta-adrenoceptor-mediated fine tuning of the T-cell differentiation/maturation. In non-Cx rats P affected TCR alpha beta-dependent stages of thymocyte differentiation/maturation decreasing frequency of CD4+8+ double positive (DP) TCR alpha beta(low) low cells entering selection processes and increasing relative number of positively selected DP TCR alpha beta(high) (most likely due to an increased thymocyte surface density of Thy-1 that is involved in negative control of TCR alpha beta-mediated signaling/selection thresholds) and the most mature CD4+8- TCR alpha beta(high) cells (including CD4+25+ regulatory cells). However, in Cx rats P failed to produce any significant changes in thymocyte subset composition. (c) 2006 Published by Elsevier B.V.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Early postnatal castration affects thymic and thymocyte noradrenaline levels and beta-adrenoceptor-mediated influence on the thymopoiesis in adult rats",
pages = "115-100",
number = "1-2",
volume = "182",
doi = "10.1016/j.jneuroim.2006.10.004"
}

Leposavić, G., Radojević, K., Vidić-Danković, B., Kosec, D., Pilipović, I.,& Perišić, M.. (2007). Early postnatal castration affects thymic and thymocyte noradrenaline levels and beta-adrenoceptor-mediated influence on the thymopoiesis in adult rats. in Journal of Neuroimmunology
Elsevier, Amsterdam., 182(1-2), 100-115.
https://doi.org/10.1016/j.jneuroim.2006.10.004

Leposavić G, Radojević K, Vidić-Danković B, Kosec D, Pilipović I, Perišić M. Early postnatal castration affects thymic and thymocyte noradrenaline levels and beta-adrenoceptor-mediated influence on the thymopoiesis in adult rats. in Journal of Neuroimmunology. 2007;182(1-2):100-115.
doi:10.1016/j.jneuroim.2006.10.004 .

Leposavić, Gordana, Radojević, Katarina, Vidić-Danković, Biljana, Kosec, Duško, Pilipović, Ivan, Perišić, Milica, "Early postnatal castration affects thymic and thymocyte noradrenaline levels and beta-adrenoceptor-mediated influence on the thymopoiesis in adult rats" in Journal of Neuroimmunology, 182, no. 1-2 (2007):100-115,
https://doi.org/10.1016/j.jneuroim.2006.10.004 . .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Arsenović-Ranin, Nevena
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Pešić, Vesna
AU  - Vidić-Danković, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Pilipović, Ivan
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/208
AB  - Adult male Wistar rats were subjected to propranolol (P, 0.40 mg/100 g/day) or saline (S) administration (controls) over 14 days. The expression of major differentiation molecules on thymocytes and Thy-1 (CD90) molecules, which are shown to adjust thymocyte sensitivity to TCR alpha beta signaling, was studied. In addition, the sensitivity of thymocytes to induction of apoptosis and concanavalin A (Con A) signaling was estimated. The thymocytes from P-treated (PT) rats exhibited an increased sensitivity to induction of apoptosis, as well as to Con A stimulation. Furthermore, P treatment produced changes in the distribution of thymocyte subsets suggesting that more cells passed positive selection and further differentiated into mature CD4+ or CD8+ single positive (SP) TCR alpha beta(high) cells. These changes may, at least partly, be related to the markedly increased density of Thy-1 surface expression on TCR alpha beta(low) thymocytes from these rats. The increased frequency of cells expressing the CD4+25+ phenotype, which has been shown to be characteristic for regulatory cells in the thymus, may also indicate alterations in thymocyte selection following P treatment. Inasmuch as positive and negative selections play an important role in continuously reshaping the T-cell repertoire and maintaining tolerance, the hereby presented study suggests that pharmacological manipulations with beta-AR signaling, or chemically evoked alterations in catecholamine release, may interfere with the regulation of thymocyte selection, and consequently with the immune response.
PB  - Springer, Dordrecht
T2  - Molecular and Cellular Biochemistry
T1  - Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis
EP  - 99
IS  - 1-2
SP  - 87
VL  - 285
DO  - 10.1007/s11010-005-9059-5
ER  - 

@article{
author = "Leposavić, Gordana and Arsenović-Ranin, Nevena and Radojević, Katarina and Kosec, Duško and Pešić, Vesna and Vidić-Danković, Biljana and Plećaš-Solarović, Bosiljka and Pilipović, Ivan",
year = "2006",
abstract = "Adult male Wistar rats were subjected to propranolol (P, 0.40 mg/100 g/day) or saline (S) administration (controls) over 14 days. The expression of major differentiation molecules on thymocytes and Thy-1 (CD90) molecules, which are shown to adjust thymocyte sensitivity to TCR alpha beta signaling, was studied. In addition, the sensitivity of thymocytes to induction of apoptosis and concanavalin A (Con A) signaling was estimated. The thymocytes from P-treated (PT) rats exhibited an increased sensitivity to induction of apoptosis, as well as to Con A stimulation. Furthermore, P treatment produced changes in the distribution of thymocyte subsets suggesting that more cells passed positive selection and further differentiated into mature CD4+ or CD8+ single positive (SP) TCR alpha beta(high) cells. These changes may, at least partly, be related to the markedly increased density of Thy-1 surface expression on TCR alpha beta(low) thymocytes from these rats. The increased frequency of cells expressing the CD4+25+ phenotype, which has been shown to be characteristic for regulatory cells in the thymus, may also indicate alterations in thymocyte selection following P treatment. Inasmuch as positive and negative selections play an important role in continuously reshaping the T-cell repertoire and maintaining tolerance, the hereby presented study suggests that pharmacological manipulations with beta-AR signaling, or chemically evoked alterations in catecholamine release, may interfere with the regulation of thymocyte selection, and consequently with the immune response.",
publisher = "Springer, Dordrecht",
journal = "Molecular and Cellular Biochemistry",
title = "Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis",
pages = "99-87",
number = "1-2",
volume = "285",
doi = "10.1007/s11010-005-9059-5"
}

Leposavić, G., Arsenović-Ranin, N., Radojević, K., Kosec, D., Pešić, V., Vidić-Danković, B., Plećaš-Solarović, B.,& Pilipović, I.. (2006). Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis. in Molecular and Cellular Biochemistry
Springer, Dordrecht., 285(1-2), 87-99.
https://doi.org/10.1007/s11010-005-9059-5

Leposavić G, Arsenović-Ranin N, Radojević K, Kosec D, Pešić V, Vidić-Danković B, Plećaš-Solarović B, Pilipović I. Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis. in Molecular and Cellular Biochemistry. 2006;285(1-2):87-99.
doi:10.1007/s11010-005-9059-5 .

Leposavić, Gordana, Arsenović-Ranin, Nevena, Radojević, Katarina, Kosec, Duško, Pešić, Vesna, Vidić-Danković, Biljana, Plećaš-Solarović, Bosiljka, Pilipović, Ivan, "Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis" in Molecular and Cellular Biochemistry, 285, no. 1-2 (2006):87-99,
https://doi.org/10.1007/s11010-005-9059-5 . .

TY  - CONF
AU  - Radojević, Katarina
AU  - Vidić-Danković, Biljana
AU  - Pilipović, Ivan
AU  - Perišić, Milica
AU  - Leposavić, Gordana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/217
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Effects of propranolol on the thymocyte maturation: Role for gonadal status
T1  - Uticaj propranolola na sazrevanje timocita - značaj gonadnog statusa
EP  - 381
IS  - 4
SP  - 380
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_intor_217
ER  - 

@conference{
author = "Radojević, Katarina and Vidić-Danković, Biljana and Pilipović, Ivan and Perišić, Milica and Leposavić, Gordana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Effects of propranolol on the thymocyte maturation: Role for gonadal status, Uticaj propranolola na sazrevanje timocita - značaj gonadnog statusa",
pages = "381-380",
number = "4",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_intor_217"
}

Radojević, K., Vidić-Danković, B., Pilipović, I., Perišić, M.,& Leposavić, G.. (2006). Effects of propranolol on the thymocyte maturation: Role for gonadal status. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 380-381.
https://hdl.handle.net/21.15107/rcub_intor_217

Radojević K, Vidić-Danković B, Pilipović I, Perišić M, Leposavić G. Effects of propranolol on the thymocyte maturation: Role for gonadal status. in Arhiv za farmaciju. 2006;56(4):380-381.
https://hdl.handle.net/21.15107/rcub_intor_217 .

Radojević, Katarina, Vidić-Danković, Biljana, Pilipović, Ivan, Perišić, Milica, Leposavić, Gordana, "Effects of propranolol on the thymocyte maturation: Role for gonadal status" in Arhiv za farmaciju, 56, no. 4 (2006):380-381,
https://hdl.handle.net/21.15107/rcub_intor_217 .

TY  - JOUR
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
AU  - Isaković, Katarina
AU  - Leposavić, Gordana
PY  - 2005
UR  - http://intor.torlakinstitut.com/handle/123456789/198
AB  - Experimental autoimmune myasthenia gravis (EAMG) was induced in female AO rats, by a single immunization with Torpedo acetylcholine receptor (AChR). Animals injected with saline served as controls. The study showed substantial changes in EAMG rats in the thymopoiesis, causing an increase in the relative proportion of mature CD8+ and, particularly, CD4+ (possibly autoreactive) single positive (SP) cells expressing TCR alpha beta at high level (TCR alpha beta(high)), as well as in that of mature double negative (DN) TCR alpha beta(high) cells, which are believed to be the immunoregulatory cells that augment autoantibody (autoAb) production. These results indicate that an augmented production of autoreactive CD4+ cells, on one side, and an increase of the immunoregulatory T cells that augment autoAb production, on the other side (reflecting, most likely, an increased entry of activated autoreactive CD4+ T cells from the periphery into the thymus), may have a significant role in the sustention of immune response in EAMG, and may suggest a putative mechanism underlying the sustention of autoimmune response in acquired MG.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Alterations in the thymopoiesis in experimental autoimmune myasthenia gravis
EP  - 477
IS  - 4
SP  - 461
VL  - 115
DO  - 10.1080/00207450590522464
ER  - 

@article{
author = "Kosec, Duško and Vidić-Danković, Biljana and Isaković, Katarina and Leposavić, Gordana",
year = "2005",
abstract = "Experimental autoimmune myasthenia gravis (EAMG) was induced in female AO rats, by a single immunization with Torpedo acetylcholine receptor (AChR). Animals injected with saline served as controls. The study showed substantial changes in EAMG rats in the thymopoiesis, causing an increase in the relative proportion of mature CD8+ and, particularly, CD4+ (possibly autoreactive) single positive (SP) cells expressing TCR alpha beta at high level (TCR alpha beta(high)), as well as in that of mature double negative (DN) TCR alpha beta(high) cells, which are believed to be the immunoregulatory cells that augment autoantibody (autoAb) production. These results indicate that an augmented production of autoreactive CD4+ cells, on one side, and an increase of the immunoregulatory T cells that augment autoAb production, on the other side (reflecting, most likely, an increased entry of activated autoreactive CD4+ T cells from the periphery into the thymus), may have a significant role in the sustention of immune response in EAMG, and may suggest a putative mechanism underlying the sustention of autoimmune response in acquired MG.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Alterations in the thymopoiesis in experimental autoimmune myasthenia gravis",
pages = "477-461",
number = "4",
volume = "115",
doi = "10.1080/00207450590522464"
}

Kosec, D., Vidić-Danković, B., Isaković, K.,& Leposavić, G.. (2005). Alterations in the thymopoiesis in experimental autoimmune myasthenia gravis. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 115(4), 461-477.
https://doi.org/10.1080/00207450590522464

Kosec D, Vidić-Danković B, Isaković K, Leposavić G. Alterations in the thymopoiesis in experimental autoimmune myasthenia gravis. in International Journal of Neuroscience. 2005;115(4):461-477.
doi:10.1080/00207450590522464 .

Kosec, Duško, Vidić-Danković, Biljana, Isaković, Katarina, Leposavić, Gordana, "Alterations in the thymopoiesis in experimental autoimmune myasthenia gravis" in International Journal of Neuroscience, 115, no. 4 (2005):461-477,
https://doi.org/10.1080/00207450590522464 . .

TY  - CONF
AU  - Vidić-Danković, Biljana
AU  - Radojević, Katarina
AU  - Leposavić, Gordana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/168
PB  - Elsevier, Amsterdam
C3  - Journal of Neuroimmunology
T1  - Orchidectomy affects the monoamine content in rat thymocytes
EP  - 130
IS  - 1-2
SP  - 130
VL  - 154
UR  - https://hdl.handle.net/21.15107/rcub_intor_168
ER  - 

@conference{
author = "Vidić-Danković, Biljana and Radojević, Katarina and Leposavić, Gordana",
year = "2004",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Orchidectomy affects the monoamine content in rat thymocytes",
pages = "130-130",
number = "1-2",
volume = "154",
url = "https://hdl.handle.net/21.15107/rcub_intor_168"
}

Vidić-Danković, B., Radojević, K.,& Leposavić, G.. (2004). Orchidectomy affects the monoamine content in rat thymocytes. in Journal of Neuroimmunology
Elsevier, Amsterdam., 154(1-2), 130-130.
https://hdl.handle.net/21.15107/rcub_intor_168

Vidić-Danković B, Radojević K, Leposavić G. Orchidectomy affects the monoamine content in rat thymocytes. in Journal of Neuroimmunology. 2004;154(1-2):130-130.
https://hdl.handle.net/21.15107/rcub_intor_168 .

Vidić-Danković, Biljana, Radojević, Katarina, Leposavić, Gordana, "Orchidectomy affects the monoamine content in rat thymocytes" in Journal of Neuroimmunology, 154, no. 1-2 (2004):130-130,
https://hdl.handle.net/21.15107/rcub_intor_168 .

TY  - JOUR
AU  - Rauški, Aleksandra
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/162
AB  - The study revealed that beta-adrenoceptor blockade with propranolol (0.40 mg/100 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of beta-adrenoceptors in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes
EP  - 1673
IS  - 12
SP  - 1653
VL  - 113
DO  - 10.1080/00207450390245216
ER  - 

@article{
author = "Rauški, Aleksandra and Kosec, Duško and Vidić-Danković, Biljana and Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2003",
abstract = "The study revealed that beta-adrenoceptor blockade with propranolol (0.40 mg/100 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of beta-adrenoceptors in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes",
pages = "1673-1653",
number = "12",
volume = "113",
doi = "10.1080/00207450390245216"
}

Rauški, A., Kosec, D., Vidić-Danković, B., Plećaš-Solarović, B.,& Leposavić, G.. (2003). Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 113(12), 1653-1673.
https://doi.org/10.1080/00207450390245216

Rauški A, Kosec D, Vidić-Danković B, Plećaš-Solarović B, Leposavić G. Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes. in International Journal of Neuroscience. 2003;113(12):1653-1673.
doi:10.1080/00207450390245216 .

Rauški, Aleksandra, Kosec, Duško, Vidić-Danković, Biljana, Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes" in International Journal of Neuroscience, 113, no. 12 (2003):1653-1673,
https://doi.org/10.1080/00207450390245216 . .

TY  - JOUR
AU  - Rauški, Aleksandra
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
AU  - Radojević, Katarina
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/161
AB  - The present study was undertaken in order to further clarify putative role of the adrenergic innervation in the regulation of the intrathymic T-cell maturation. For this purpose adult male DA rats were subjected to either 4-day- or 16-day-long propranolol treatment (0.40 mg propranolol/100 g/day, s.c.) and the expression of CD4/8/TCRalphabeta on thymocytes, as well as thymocyte proliferative and apoptotic index, was assessed in these animals by flow cytometric analysis. Propranolol treatment, in spite of duration, increased both the thymocyte proliferative and apoptotic index (vs. respective vehicle-treated controls). In 4-day-treated animals the thymus cellularity and thymus weight remained unaltered, while in 16-day-treated rats the values of both of these parameters were reduced (since increase in the thymocyte apoptotic index overcame that in the proliferative index). The treatments of both durations affected the thymocyte phenotypic profile in a similar pattern, but the changes were more pronounced in rats exposed to the treatment of longer duration. The relative proportion of the least mature CD4-8- double negative (DN) TCRalphabeta(-) cells was increased, those of thymocytes at distinct differentiational stages on the transitional route to the CD4+8+ double positive (DP) TCRalphabeta(low) stage decreased (all subsets of TCRalphabeta(-) in both groups of rats, and those. with low expression of TCRalphabeta in rats subjected to 16-day-long treatment) or unaltered (all subsets of TCRalphabeta(low) cells in 4-day-treated rats). Furthermore, the percentage of CD4+8+ DP TCRalphabeta(low) cells was significantly elevated, as well as those of the most mature CD4+8-TCRalphabeta(high) and CD4-8+TCRalphabeta(high) cells (the increase in the percentage of former was much more conspicuous than that of the latter), while the relative proportion of their direct detectable precursors (CD4+8+ DP TCRalphabeta(high)) Was reduced. Thus, the present study: i) further supports notion of pharmacological manipulation of adrenergic action as an efficient means in modulation of the T-cell development, and hence T-cell-dependent immune response, and ii) provides more specific insight into T-cell maturation sequence point/s particularly sensitive to beta-adrenoceptor ligand action.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Immunopharmacology and Immunotoxicology
T1  - Thymopoiesis following chronic blockade of beta-adrenoceptors
EP  - 528
IS  - 4
SP  - 513
VL  - 25
DO  - 10.1081/IPH-120026437
ER  - 

@article{
author = "Rauški, Aleksandra and Kosec, Duško and Vidić-Danković, Biljana and Radojević, Katarina and Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2003",
abstract = "The present study was undertaken in order to further clarify putative role of the adrenergic innervation in the regulation of the intrathymic T-cell maturation. For this purpose adult male DA rats were subjected to either 4-day- or 16-day-long propranolol treatment (0.40 mg propranolol/100 g/day, s.c.) and the expression of CD4/8/TCRalphabeta on thymocytes, as well as thymocyte proliferative and apoptotic index, was assessed in these animals by flow cytometric analysis. Propranolol treatment, in spite of duration, increased both the thymocyte proliferative and apoptotic index (vs. respective vehicle-treated controls). In 4-day-treated animals the thymus cellularity and thymus weight remained unaltered, while in 16-day-treated rats the values of both of these parameters were reduced (since increase in the thymocyte apoptotic index overcame that in the proliferative index). The treatments of both durations affected the thymocyte phenotypic profile in a similar pattern, but the changes were more pronounced in rats exposed to the treatment of longer duration. The relative proportion of the least mature CD4-8- double negative (DN) TCRalphabeta(-) cells was increased, those of thymocytes at distinct differentiational stages on the transitional route to the CD4+8+ double positive (DP) TCRalphabeta(low) stage decreased (all subsets of TCRalphabeta(-) in both groups of rats, and those. with low expression of TCRalphabeta in rats subjected to 16-day-long treatment) or unaltered (all subsets of TCRalphabeta(low) cells in 4-day-treated rats). Furthermore, the percentage of CD4+8+ DP TCRalphabeta(low) cells was significantly elevated, as well as those of the most mature CD4+8-TCRalphabeta(high) and CD4-8+TCRalphabeta(high) cells (the increase in the percentage of former was much more conspicuous than that of the latter), while the relative proportion of their direct detectable precursors (CD4+8+ DP TCRalphabeta(high)) Was reduced. Thus, the present study: i) further supports notion of pharmacological manipulation of adrenergic action as an efficient means in modulation of the T-cell development, and hence T-cell-dependent immune response, and ii) provides more specific insight into T-cell maturation sequence point/s particularly sensitive to beta-adrenoceptor ligand action.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Immunopharmacology and Immunotoxicology",
title = "Thymopoiesis following chronic blockade of beta-adrenoceptors",
pages = "528-513",
number = "4",
volume = "25",
doi = "10.1081/IPH-120026437"
}

Rauški, A., Kosec, D., Vidić-Danković, B., Radojević, K., Plećaš-Solarović, B.,& Leposavić, G.. (2003). Thymopoiesis following chronic blockade of beta-adrenoceptors. in Immunopharmacology and Immunotoxicology
Taylor & Francis Ltd, Abingdon., 25(4), 513-528.
https://doi.org/10.1081/IPH-120026437

Rauški A, Kosec D, Vidić-Danković B, Radojević K, Plećaš-Solarović B, Leposavić G. Thymopoiesis following chronic blockade of beta-adrenoceptors. in Immunopharmacology and Immunotoxicology. 2003;25(4):513-528.
doi:10.1081/IPH-120026437 .

Rauški, Aleksandra, Kosec, Duško, Vidić-Danković, Biljana, Radojević, Katarina, Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Thymopoiesis following chronic blockade of beta-adrenoceptors" in Immunopharmacology and Immunotoxicology, 25, no. 4 (2003):513-528,
https://doi.org/10.1081/IPH-120026437 . .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Pejčić-Karapetrović, Branka
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/130
AB  - In order to elucidate the features of putative age-related changes in the intrathymic T cell maturation sequence, the thymus weight, thymocyte yield and relative proportions of thymocyte subsets were analyzed at distinct maturational stages (delineated by analysis of the expression of CD4/CD8 coreceptor molecules and TCR molecular complex) in male AO rats, from the peripubertal period (1 month of age) until 10 months of age. In 2-month-old rats both the thymus weight and lymphoid content were greater than in 1-month-old rats. The values of both parameters in 4-month-old rats were reduced to the corresponding values in 1-month-old rats. These levels were sustained until 10 months of age. However, the thymocyte composition was subjected to substantial changes during the whole period studied, probably due to alterations in action of both extrinsic and intrinsic factors which influence the ability of the thymus to support T cell maturation and/or feedback regulatory action of intrathymic CD4+ T cells on thymocytopoiesis (between the age of 7 and 10 months).
AB  - U cilju definisanja karakteristika procesa sazrevanja T ćelija u timusu u funkciji uzrasta, u mužjaka pacova AO soja, različitog uzrasta (od 1 meseca do 10 meseci) posmatrane su: težina timusa, ukupan broj timocita, kao i relativna zastupljenost pojedinih timocitnih subpopulacija (koje su razdvojene na osnovu ekspresije CD4/8 koreceptornih molekula i antigena TCRαβ molekulskog kompleksa). U životinja uzrasta 2 meseca i težina timusa i ukupan broj timocita bili su značajno veći nego u životinja uzrasta od mesec dana. Međutim, u životinja uzrasta od 4 meseca vrednost ovih parametra nije se razlikovala od vrednosti odgovarajućih parametara sa mesec dana. Vrednosti oba ova parametra nisu se bitno menjale od 4. do 10. meseca života. Sa druge strane, naši rezultati su pokazali značajne promene u relativnom odnosu timocitnih subpopulacija u ispitivanom periodu. Ove promene se mogu objasniti promenama u delovanju, kako spoljašnjih tako i unutrašnjih, faktora koji modulišu sposobnost timusne mikrosredine da podrži proces sazrevanja T ćelija i/ili utiču na efikasnost regulatornog delovanja intratimusnih CD4+ T ćelija, mehanizmom negativne povratne sprege, na proces sazrevanja T ćelija u timusu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Age-associated reshaping of the thymocyte differentiation model in male rats
T1  - Promene u modelu diferentovanja timocita u mužjaka pacova sa godinama
EP  - 217
IS  - 4
SP  - 203
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_intor_130
ER  - 

@article{
author = "Leposavić, Gordana and Pejčić-Karapetrović, Branka and Kosec, Duško and Vidić-Danković, Biljana",
year = "2001",
abstract = "In order to elucidate the features of putative age-related changes in the intrathymic T cell maturation sequence, the thymus weight, thymocyte yield and relative proportions of thymocyte subsets were analyzed at distinct maturational stages (delineated by analysis of the expression of CD4/CD8 coreceptor molecules and TCR molecular complex) in male AO rats, from the peripubertal period (1 month of age) until 10 months of age. In 2-month-old rats both the thymus weight and lymphoid content were greater than in 1-month-old rats. The values of both parameters in 4-month-old rats were reduced to the corresponding values in 1-month-old rats. These levels were sustained until 10 months of age. However, the thymocyte composition was subjected to substantial changes during the whole period studied, probably due to alterations in action of both extrinsic and intrinsic factors which influence the ability of the thymus to support T cell maturation and/or feedback regulatory action of intrathymic CD4+ T cells on thymocytopoiesis (between the age of 7 and 10 months)., U cilju definisanja karakteristika procesa sazrevanja T ćelija u timusu u funkciji uzrasta, u mužjaka pacova AO soja, različitog uzrasta (od 1 meseca do 10 meseci) posmatrane su: težina timusa, ukupan broj timocita, kao i relativna zastupljenost pojedinih timocitnih subpopulacija (koje su razdvojene na osnovu ekspresije CD4/8 koreceptornih molekula i antigena TCRαβ molekulskog kompleksa). U životinja uzrasta 2 meseca i težina timusa i ukupan broj timocita bili su značajno veći nego u životinja uzrasta od mesec dana. Međutim, u životinja uzrasta od 4 meseca vrednost ovih parametra nije se razlikovala od vrednosti odgovarajućih parametara sa mesec dana. Vrednosti oba ova parametra nisu se bitno menjale od 4. do 10. meseca života. Sa druge strane, naši rezultati su pokazali značajne promene u relativnom odnosu timocitnih subpopulacija u ispitivanom periodu. Ove promene se mogu objasniti promenama u delovanju, kako spoljašnjih tako i unutrašnjih, faktora koji modulišu sposobnost timusne mikrosredine da podrži proces sazrevanja T ćelija i/ili utiču na efikasnost regulatornog delovanja intratimusnih CD4+ T ćelija, mehanizmom negativne povratne sprege, na proces sazrevanja T ćelija u timusu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Age-associated reshaping of the thymocyte differentiation model in male rats, Promene u modelu diferentovanja timocita u mužjaka pacova sa godinama",
pages = "217-203",
number = "4",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_intor_130"
}

Leposavić, G., Pejčić-Karapetrović, B., Kosec, D.,& Vidić-Danković, B.. (2001). Age-associated reshaping of the thymocyte differentiation model in male rats. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 51(4), 203-217.
https://hdl.handle.net/21.15107/rcub_intor_130

Leposavić G, Pejčić-Karapetrović B, Kosec D, Vidić-Danković B. Age-associated reshaping of the thymocyte differentiation model in male rats. in Acta veterinaria - Beograd. 2001;51(4):203-217.
https://hdl.handle.net/21.15107/rcub_intor_130 .

Leposavić, Gordana, Pejčić-Karapetrović, Branka, Kosec, Duško, Vidić-Danković, Biljana, "Age-associated reshaping of the thymocyte differentiation model in male rats" in Acta veterinaria - Beograd, 51, no. 4 (2001):203-217,
https://hdl.handle.net/21.15107/rcub_intor_130 .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Obradović, S.
AU  - Kosec, Duško
AU  - Pejčić-Karapetrović, Branka
AU  - Vidić-Danković, Biljana
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/129
AB  - This study examined the effects of the principal ovarian steroids, 17β-estradiol (E) and progesterone (P), on the thymic structure and on the intrathymic development of T-cells. Adult female rats were ovariectomized (OVX) and treated for 14 days with physiological doses of either E or P; controls received an equivalent volume of vehicle. Ovariectomy produced a marked increase (vs. sham-operated controls) in thymus weight, which was associated with an increase in the volume and cellularity of both the medulla and cortex. Treatment of OVX rats with E reduced the thymic weight to value, which was significantly lower than that of sham-operated controls decreasing the volume of cortex below level in sham-OVX rats, and reversing the effect of ovariectomy on the volume of medulla. P only prevented the increases in thymus weight and cortical volume induced by OVX. However, unlike E, it had no discernable effect on the medullary volume. E treatment reduced the cellularity of the cortex and medulla to values, which were lower than those of sham-OVX rats, while P only reversed the effects of OVX on the cellularity of both the compartments. Ovariectomy also had a profound effect on the thymocyte profile, increasing the proportion of CD4 + 8 + TCRαβ - cells and producing a corresponding decrease in the relative proportions of all TCR αβ high cell subsets. The decrease in the latter was opposed by treatment with E or P. However, the sensitivity of the less mature cells (except CD4 - 8 - TCR αβ - , the percentage of which was reduced by both hormones) to the two hormones differed. E reduced the relative proportion of CD4 - 8 + TCRαβ - , CD4 - 8 + TCRαβ low and CD4 + 8 + TCRαβ - cells, while P increased the percentage of CD4 - 8 + TCRαβ low cells. The results suggest that E and P affect both the lymphoid and nonlymphoid compartments of the thymus, and that while P increases the volume of the nonlymphoid component of the medulla, E has the opposite effect. The finding that ovariectomy decreased while E and P increased the relative proportion of the most mature thymocytes, which include CD4 - 8 - TCRαβ high cells that are believed to harbour potentially autoreactive cell clones, is particularly interesting and may relate to the high propensity of autoimmune diseases in females.
PB  - Elsevier
T2  - International Immunopharmacology
T1  - In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones
EP  - 12
IS  - 1
SP  - 1
VL  - 1
DO  - 10.1016/S1567-5769(00)00006-0
ER  - 

@article{
author = "Leposavić, Gordana and Obradović, S. and Kosec, Duško and Pejčić-Karapetrović, Branka and Vidić-Danković, Biljana",
year = "2001",
abstract = "This study examined the effects of the principal ovarian steroids, 17β-estradiol (E) and progesterone (P), on the thymic structure and on the intrathymic development of T-cells. Adult female rats were ovariectomized (OVX) and treated for 14 days with physiological doses of either E or P; controls received an equivalent volume of vehicle. Ovariectomy produced a marked increase (vs. sham-operated controls) in thymus weight, which was associated with an increase in the volume and cellularity of both the medulla and cortex. Treatment of OVX rats with E reduced the thymic weight to value, which was significantly lower than that of sham-operated controls decreasing the volume of cortex below level in sham-OVX rats, and reversing the effect of ovariectomy on the volume of medulla. P only prevented the increases in thymus weight and cortical volume induced by OVX. However, unlike E, it had no discernable effect on the medullary volume. E treatment reduced the cellularity of the cortex and medulla to values, which were lower than those of sham-OVX rats, while P only reversed the effects of OVX on the cellularity of both the compartments. Ovariectomy also had a profound effect on the thymocyte profile, increasing the proportion of CD4 + 8 + TCRαβ - cells and producing a corresponding decrease in the relative proportions of all TCR αβ high cell subsets. The decrease in the latter was opposed by treatment with E or P. However, the sensitivity of the less mature cells (except CD4 - 8 - TCR αβ - , the percentage of which was reduced by both hormones) to the two hormones differed. E reduced the relative proportion of CD4 - 8 + TCRαβ - , CD4 - 8 + TCRαβ low and CD4 + 8 + TCRαβ - cells, while P increased the percentage of CD4 - 8 + TCRαβ low cells. The results suggest that E and P affect both the lymphoid and nonlymphoid compartments of the thymus, and that while P increases the volume of the nonlymphoid component of the medulla, E has the opposite effect. The finding that ovariectomy decreased while E and P increased the relative proportion of the most mature thymocytes, which include CD4 - 8 - TCRαβ high cells that are believed to harbour potentially autoreactive cell clones, is particularly interesting and may relate to the high propensity of autoimmune diseases in females.",
publisher = "Elsevier",
journal = "International Immunopharmacology",
title = "In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones",
pages = "12-1",
number = "1",
volume = "1",
doi = "10.1016/S1567-5769(00)00006-0"
}

Leposavić, G., Obradović, S., Kosec, D., Pejčić-Karapetrović, B.,& Vidić-Danković, B.. (2001). In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones. in International Immunopharmacology
Elsevier., 1(1), 1-12.
https://doi.org/10.1016/S1567-5769(00)00006-0

Leposavić G, Obradović S, Kosec D, Pejčić-Karapetrović B, Vidić-Danković B. In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones. in International Immunopharmacology. 2001;1(1):1-12.
doi:10.1016/S1567-5769(00)00006-0 .

Leposavić, Gordana, Obradović, S., Kosec, Duško, Pejčić-Karapetrović, Branka, Vidić-Danković, Biljana, "In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones" in International Immunopharmacology, 1, no. 1 (2001):1-12,
https://doi.org/10.1016/S1567-5769(00)00006-0 . .

TY  - JOUR
AU  - Obradović, S.
AU  - Vidić-Danković, Biljana
AU  - Pejčić-Karapetrović, Branka
AU  - Kosec, Duško
AU  - Leposavić, Gordana
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/126
AB  - The study was designed to shed more light on the controversial role of the two main ovarian steroid hormones (i.e. estradiol and progesterone) in shaping the size and phenotypic characteristics of the splenic lymphocyte pool. For this purpose ovariectomized adult rats (OVX) were treated for 14 subsequent days with either estradiol or progesterone (to attain physiological concentrations of the hormones). Afterwards, the splenocyte yield, and overall number of splenocytes bearing TCR alpha beta receptor, CD4 and CD8 coreceptor were evaluated. Fourteen-day-long ovarian hormone deprivation produced an increase in the splenic weight and splenocyte yield ton the account of a rise in the number of TCR alpha beta (-) cells), although the number of TCR alpha beta (+) cells was reduced as a result of a decrease in the size of the CD4(+) cell subpopulation. Replacement of either estradiol or progesterone prevented the increase hn splenic weight and reduced the splenocyte yield to values significantly lower than that in sham-OVX rats. Both the treatments completely abolished the effect of ovariectomy on the size of TCR alpha beta (-) cell population, but had differential effects on that of TCR alpha beta (+) cell population: estradiol did not affect its size, while progesterone caused a reduction on the account of a decrease in the numbers of both CD4+ and CD8(+) cells. The results suggest that: a) estradiol and progesterone have similar effects on the size of the splenic B cell population and that replacement of either estradiol or progesterone can prevent the effects of ovariectomy on the size of this population and b) estradiol does not affect while progesterone reduces the size of splenic T cell population. Thus, replacement of none of them is able to compensate the removal of gonads.
PB  - Govi-Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - In vivo modulation of the splenocyte yield and composition by female sex steroid hormones
EP  - 238
IS  - 3
SP  - 235
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_intor_126
ER  - 

@article{
author = "Obradović, S. and Vidić-Danković, Biljana and Pejčić-Karapetrović, Branka and Kosec, Duško and Leposavić, Gordana",
year = "2001",
abstract = "The study was designed to shed more light on the controversial role of the two main ovarian steroid hormones (i.e. estradiol and progesterone) in shaping the size and phenotypic characteristics of the splenic lymphocyte pool. For this purpose ovariectomized adult rats (OVX) were treated for 14 subsequent days with either estradiol or progesterone (to attain physiological concentrations of the hormones). Afterwards, the splenocyte yield, and overall number of splenocytes bearing TCR alpha beta receptor, CD4 and CD8 coreceptor were evaluated. Fourteen-day-long ovarian hormone deprivation produced an increase in the splenic weight and splenocyte yield ton the account of a rise in the number of TCR alpha beta (-) cells), although the number of TCR alpha beta (+) cells was reduced as a result of a decrease in the size of the CD4(+) cell subpopulation. Replacement of either estradiol or progesterone prevented the increase hn splenic weight and reduced the splenocyte yield to values significantly lower than that in sham-OVX rats. Both the treatments completely abolished the effect of ovariectomy on the size of TCR alpha beta (-) cell population, but had differential effects on that of TCR alpha beta (+) cell population: estradiol did not affect its size, while progesterone caused a reduction on the account of a decrease in the numbers of both CD4+ and CD8(+) cells. The results suggest that: a) estradiol and progesterone have similar effects on the size of the splenic B cell population and that replacement of either estradiol or progesterone can prevent the effects of ovariectomy on the size of this population and b) estradiol does not affect while progesterone reduces the size of splenic T cell population. Thus, replacement of none of them is able to compensate the removal of gonads.",
publisher = "Govi-Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "In vivo modulation of the splenocyte yield and composition by female sex steroid hormones",
pages = "238-235",
number = "3",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_intor_126"
}

Obradović, S., Vidić-Danković, B., Pejčić-Karapetrović, B., Kosec, D.,& Leposavić, G.. (2001). In vivo modulation of the splenocyte yield and composition by female sex steroid hormones. in Pharmazie
Govi-Verlag Gmbh, Eschborn., 56(3), 235-238.
https://hdl.handle.net/21.15107/rcub_intor_126

Obradović S, Vidić-Danković B, Pejčić-Karapetrović B, Kosec D, Leposavić G. In vivo modulation of the splenocyte yield and composition by female sex steroid hormones. in Pharmazie. 2001;56(3):235-238.
https://hdl.handle.net/21.15107/rcub_intor_126 .

Obradović, S., Vidić-Danković, Biljana, Pejčić-Karapetrović, Branka, Kosec, Duško, Leposavić, Gordana, "In vivo modulation of the splenocyte yield and composition by female sex steroid hormones" in Pharmazie, 56, no. 3 (2001):235-238,
https://hdl.handle.net/21.15107/rcub_intor_126 .