TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Prijić, Ivana
AU  - Vujić, Vesna
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/511
AB  - The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute to better understanding of the complex role(s) that E2 may play in innate immunity during aging, and that are dependent of sex.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats
EP  - 94
SP  - 86
VL  - 113
DO  - 10.1016/j.exger.2018.09.024
ER  - 

@article{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Petrović, Raisa and Prijić, Ivana and Vujić, Vesna",
year = "2018",
abstract = "The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute to better understanding of the complex role(s) that E2 may play in innate immunity during aging, and that are dependent of sex.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats",
pages = "94-86",
volume = "113",
doi = "10.1016/j.exger.2018.09.024"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Petrović, R., Prijić, I.,& Vujić, V.. (2018). The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 113, 86-94.
https://doi.org/10.1016/j.exger.2018.09.024

Stanojević S, Ćuruvija I, Blagojević V, Petrović R, Prijić I, Vujić V. The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats. in Experimental Gerontology. 2018;113:86-94.
doi:10.1016/j.exger.2018.09.024 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, "The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats" in Experimental Gerontology, 113 (2018):86-94,
https://doi.org/10.1016/j.exger.2018.09.024 . .

TY  - JOUR
AU  - Blagojević, Veljko
AU  - Kovačević-Jovanović, Vesna
AU  - Ćuruvija, Ivana
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/512
AB  - Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?
EP  - 157
SP  - 147
VL  - 197
DO  - 10.1016/j.lfs.2018.02.011
ER  - 

@article{
author = "Blagojević, Veljko and Kovačević-Jovanović, Vesna and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, Ivana and Vujić, Vesna and Stanojević, Stanislava",
year = "2018",
abstract = "Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?",
pages = "157-147",
volume = "197",
doi = "10.1016/j.lfs.2018.02.011"
}

Blagojević, V., Kovačević-Jovanović, V., Ćuruvija, I., Petrović, R., Vujnović, I., Vujić, V.,& Stanojević, S.. (2018). Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 197, 147-157.
https://doi.org/10.1016/j.lfs.2018.02.011

Blagojević V, Kovačević-Jovanović V, Ćuruvija I, Petrović R, Vujnović I, Vujić V, Stanojević S. Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences. 2018;197:147-157.
doi:10.1016/j.lfs.2018.02.011 .

Blagojević, Veljko, Kovačević-Jovanović, Vesna, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?" in Life Sciences, 197 (2018):147-157,
https://doi.org/10.1016/j.lfs.2018.02.011 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Veljović, Katarina
AU  - Soković-Bajić, Svetlana
AU  - Kotur-Stevuljević, Jelena
AU  - Bogdanović, Andrija
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Kovačević-Jovanović, Vesna
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/505
AB  - The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis
EP  - 105
SP  - 92
VL  - 48
DO  - 10.1016/j.jff.2018.07.014
ER  - 

@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Veljović, Katarina and Soković-Bajić, Svetlana and Kotur-Stevuljević, Jelena and Bogdanović, Andrija and Petrović, Raisa and Vujnović, Ivana and Kovačević-Jovanović, Vesna",
year = "2018",
abstract = "The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis",
pages = "105-92",
volume = "48",
doi = "10.1016/j.jff.2018.07.014"
}

Stanojević, S., Blagojević, V., Ćuruvija, I., Veljović, K., Soković-Bajić, S., Kotur-Stevuljević, J., Bogdanović, A., Petrović, R., Vujnović, I.,& Kovačević-Jovanović, V.. (2018). Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 48, 92-105.
https://doi.org/10.1016/j.jff.2018.07.014

Stanojević S, Blagojević V, Ćuruvija I, Veljović K, Soković-Bajić S, Kotur-Stevuljević J, Bogdanović A, Petrović R, Vujnović I, Kovačević-Jovanović V. Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods. 2018;48:92-105.
doi:10.1016/j.jff.2018.07.014 .

Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Veljović, Katarina, Soković-Bajić, Svetlana, Kotur-Stevuljević, Jelena, Bogdanović, Andrija, Petrović, Raisa, Vujnović, Ivana, Kovačević-Jovanović, Vesna, "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis" in Journal of Functional Foods, 48 (2018):92-105,
https://doi.org/10.1016/j.jff.2018.07.014 . .

TY  - JOUR
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Arsenović-Ranin, Nevena
AU  - Blagojević, Veljko
AU  - Dimitrijević, Mirjana
AU  - Vidić-Danković, Biljana
AU  - Vujić, Vesna
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/496
AB  - The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression
EP  - 1101
IS  - 3
SP  - 1087
VL  - 40
DO  - 10.1007/s10753-017-0551-3
ER  - 

@article{
author = "Ćuruvija, Ivana and Stanojević, Stanislava and Arsenović-Ranin, Nevena and Blagojević, Veljko and Dimitrijević, Mirjana and Vidić-Danković, Biljana and Vujić, Vesna",
year = "2017",
abstract = "The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression",
pages = "1101-1087",
number = "3",
volume = "40",
doi = "10.1007/s10753-017-0551-3"
}

Ćuruvija, I., Stanojević, S., Arsenović-Ranin, N., Blagojević, V., Dimitrijević, M., Vidić-Danković, B.,& Vujić, V.. (2017). Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation
Springer/Plenum Publishers, New York., 40(3), 1087-1101.
https://doi.org/10.1007/s10753-017-0551-3

Ćuruvija I, Stanojević S, Arsenović-Ranin N, Blagojević V, Dimitrijević M, Vidić-Danković B, Vujić V. Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation. 2017;40(3):1087-1101.
doi:10.1007/s10753-017-0551-3 .

Ćuruvija, Ivana, Stanojević, Stanislava, Arsenović-Ranin, Nevena, Blagojević, Veljko, Dimitrijević, Mirjana, Vidić-Danković, Biljana, Vujić, Vesna, "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression" in Inflammation, 40, no. 3 (2017):1087-1101,
https://doi.org/10.1007/s10753-017-0551-3 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/453
AB  - Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-alpha, and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-alpha production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging
EP  - 107
SP  - 95
VL  - 85
DO  - 10.1016/j.exger.2016.10.005
ER  - 

@article{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Petrović, Raisa and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2016",
abstract = "Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-alpha, and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-alpha production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging",
pages = "107-95",
volume = "85",
doi = "10.1016/j.exger.2016.10.005"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Petrović, R., Vujić, V.,& Dimitrijević, M.. (2016). Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 85, 95-107.
https://doi.org/10.1016/j.exger.2016.10.005

Stanojević S, Ćuruvija I, Blagojević V, Petrović R, Vujić V, Dimitrijević M. Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging. in Experimental Gerontology. 2016;85:95-107.
doi:10.1016/j.exger.2016.10.005 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Vujić, Vesna, Dimitrijević, Mirjana, "Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging" in Experimental Gerontology, 85 (2016):95-107,
https://doi.org/10.1016/j.exger.2016.10.005 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujnović, Ivana
AU  - Petrović, Raisa
AU  - Arsenović-Ranin, Nevena
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/466
AB  - Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age.
PB  - Springer, New York
T2  - Biogerontology
T1  - Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4
EP  - 371
IS  - 2
SP  - 359
VL  - 17
DO  - 10.1007/s10522-015-9620-x
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujnović, Ivana and Petrović, Raisa and Arsenović-Ranin, Nevena and Vujić, Vesna and Leposavić, Gordana",
year = "2016",
abstract = "Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age.",
publisher = "Springer, New York",
journal = "Biogerontology",
title = "Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4",
pages = "371-359",
number = "2",
volume = "17",
doi = "10.1007/s10522-015-9620-x"
}

Dimitrijević, M., Stanojević, S., Blagojević, V., Ćuruvija, I., Vujnović, I., Petrović, R., Arsenović-Ranin, N., Vujić, V.,& Leposavić, G.. (2016). Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4. in Biogerontology
Springer, New York., 17(2), 359-371.
https://doi.org/10.1007/s10522-015-9620-x

Dimitrijević M, Stanojević S, Blagojević V, Ćuruvija I, Vujnović I, Petrović R, Arsenović-Ranin N, Vujić V, Leposavić G. Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4. in Biogerontology. 2016;17(2):359-371.
doi:10.1007/s10522-015-9620-x .

Dimitrijević, Mirjana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujnović, Ivana, Petrović, Raisa, Arsenović-Ranin, Nevena, Vujić, Vesna, Leposavić, Gordana, "Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4" in Biogerontology, 17, no. 2 (2016):359-371,
https://doi.org/10.1007/s10522-015-9620-x . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/423
AB  - Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat
EP  - 456
IS  - 5
SP  - 445
VL  - 74
DO  - 10.1111/aji.12424
ER  - 

@article{
author = "Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana and Vujić, Vesna and Ćuruvija, Ivana and Blagojević, Veljko and Leposavić, Gordana",
year = "2015",
abstract = "Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat",
pages = "456-445",
number = "5",
volume = "74",
doi = "10.1111/aji.12424"
}

Stanojević, S., Kovačević-Jovanović, V., Dimitrijević, M., Vujić, V., Ćuruvija, I., Blagojević, V.,& Leposavić, G.. (2015). Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology
Wiley, Hoboken., 74(5), 445-456.
https://doi.org/10.1111/aji.12424

Stanojević S, Kovačević-Jovanović V, Dimitrijević M, Vujić V, Ćuruvija I, Blagojević V, Leposavić G. Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology. 2015;74(5):445-456.
doi:10.1111/aji.12424 .

Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, Vujić, Vesna, Ćuruvija, Ivana, Blagojević, Veljko, Leposavić, Gordana, "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat" in American Journal of Reproductive Immunology, 74, no. 5 (2015):445-456,
https://doi.org/10.1111/aji.12424 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/424
AB  - This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains
EP  - 1449
IS  - 4
SP  - 1434
VL  - 38
DO  - 10.1007/s10753-015-0118-0
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2015",
abstract = "This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains",
pages = "1449-1434",
number = "4",
volume = "38",
doi = "10.1007/s10753-015-0118-0"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V.,& Dimitrijević, M.. (2015). Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation
Springer/Plenum Publishers, New York., 38(4), 1434-1449.
https://doi.org/10.1007/s10753-015-0118-0

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Dimitrijević M. Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation. 2015;38(4):1434-1449.
doi:10.1007/s10753-015-0118-0 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains" in Inflammation, 38, no. 4 (2015):1434-1449,
https://doi.org/10.1007/s10753-015-0118-0 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/449
AB  - We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats
EP  - 19
IS  - 1
SP  - 1
VL  - 62
DO  - 10.1556/AMicr.62.2015.1.1
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2015",
abstract = "We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats",
pages = "19-1",
number = "1",
volume = "62",
doi = "10.1556/AMicr.62.2015.1.1"
}

Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2015). Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Zrt, Budapest., 62(1), 1-19.
https://doi.org/10.1556/AMicr.62.2015.1.1

Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica. 2015;62(1):1-19.
doi:10.1556/AMicr.62.2015.1.1 .

Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats" in Acta Microbiologica et Immunologica Hungarica, 62, no. 1 (2015):1-19,
https://doi.org/10.1556/AMicr.62.2015.1.1 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Aleksić, Iva
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Pilipović, Ivan
AU  - von Hoersten, Stephan
AU  - Leposavić, Gordana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/397
AB  - In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6-(adult), 24-(old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1 beta and IL-6) and antiinflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-alpha and IL-6 mRNAs, but greater amount of IL-1 beta mRNA. On the other hand, cells fromlong-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1 beta mRNA relative to adult rats. Consequently, IL-10/IL-1 beta mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained = 95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-alpha and IL-6 concentrations were lower; IL-1 beta concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from longlived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.
PB  - Springer, Dordrecht
T2  - AGE
T1  - Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro
IS  - 4
VL  - 36
DO  - 10.1007/s11357-014-9696-2
ER  - 

@article{
author = "Dimitrijević, Mirjana and Aleksić, Iva and Vujić, Vesna and Stanojević, Stanislava and Pilipović, Ivan and von Hoersten, Stephan and Leposavić, Gordana",
year = "2014",
abstract = "In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6-(adult), 24-(old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1 beta and IL-6) and antiinflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-alpha and IL-6 mRNAs, but greater amount of IL-1 beta mRNA. On the other hand, cells fromlong-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1 beta mRNA relative to adult rats. Consequently, IL-10/IL-1 beta mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained = 95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-alpha and IL-6 concentrations were lower; IL-1 beta concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from longlived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.",
publisher = "Springer, Dordrecht",
journal = "AGE",
title = "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro",
number = "4",
volume = "36",
doi = "10.1007/s11357-014-9696-2"
}

Dimitrijević, M., Aleksić, I., Vujić, V., Stanojević, S., Pilipović, I., von Hoersten, S.,& Leposavić, G.. (2014). Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. in AGE
Springer, Dordrecht., 36(4).
https://doi.org/10.1007/s11357-014-9696-2

Dimitrijević M, Aleksić I, Vujić V, Stanojević S, Pilipović I, von Hoersten S, Leposavić G. Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. in AGE. 2014;36(4).
doi:10.1007/s11357-014-9696-2 .

Dimitrijević, Mirjana, Aleksić, Iva, Vujić, Vesna, Stanojević, Stanislava, Pilipović, Ivan, von Hoersten, Stephan, Leposavić, Gordana, "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro" in AGE, 36, no. 4 (2014),
https://doi.org/10.1007/s11357-014-9696-2 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/402
AB  - Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.
PB  - Springer, New York
T2  - Biogerontology
T1  - Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains
EP  - 486
IS  - 5
SP  - 475
VL  - 15
DO  - 10.1007/s10522-014-9513-4
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Vujić, Vesna and Aleksić, Iva and Pilipović, Ivan and Leposavić, Gordana",
year = "2014",
abstract = "Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.",
publisher = "Springer, New York",
journal = "Biogerontology",
title = "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains",
pages = "486-475",
number = "5",
volume = "15",
doi = "10.1007/s10522-014-9513-4"
}

Dimitrijević, M., Stanojević, S., Vujić, V., Aleksić, I., Pilipović, I.,& Leposavić, G.. (2014). Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology
Springer, New York., 15(5), 475-486.
https://doi.org/10.1007/s10522-014-9513-4

Dimitrijević M, Stanojević S, Vujić V, Aleksić I, Pilipović I, Leposavić G. Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology. 2014;15(5):475-486.
doi:10.1007/s10522-014-9513-4 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Vujić, Vesna, Aleksić, Iva, Pilipović, Ivan, Leposavić, Gordana, "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains" in Biogerontology, 15, no. 5 (2014):475-486,
https://doi.org/10.1007/s10522-014-9513-4 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/382
AB  - Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats
EP  - 572
IS  - 16
SP  - 564
VL  - 93
DO  - 10.1016/j.lfs.2013.08.021
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Dimitrijević, Mirjana",
year = "2013",
abstract = "Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats",
pages = "572-564",
number = "16",
volume = "93",
doi = "10.1016/j.lfs.2013.08.021"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I.,& Dimitrijević, M.. (2013). Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 93(16), 564-572.
https://doi.org/10.1016/j.lfs.2013.08.021

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Dimitrijević M. Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats. in Life Sciences. 2013;93(16):564-572.
doi:10.1016/j.lfs.2013.08.021 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Dimitrijević, Mirjana, "Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats" in Life Sciences, 93, no. 16 (2013):564-572,
https://doi.org/10.1016/j.lfs.2013.08.021 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Radojević, Katarina
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/383
AB  - The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production
EP  - 1254
IS  - 11
SP  - 1243
VL  - 48
DO  - 10.1016/j.exger.2013.07.001
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Radojević, Katarina and Leposavić, Gordana",
year = "2013",
abstract = "The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production",
pages = "1254-1243",
number = "11",
volume = "48",
doi = "10.1016/j.exger.2013.07.001"
}

Dimitrijević, M., Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I., Radojević, K.,& Leposavić, G.. (2013). The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 48(11), 1243-1254.
https://doi.org/10.1016/j.exger.2013.07.001

Dimitrijević M, Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Radojević K, Leposavić G. The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology. 2013;48(11):1243-1254.
doi:10.1016/j.exger.2013.07.001 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Radojević, Katarina, Leposavić, Gordana, "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production" in Experimental Gerontology, 48, no. 11 (2013):1243-1254,
https://doi.org/10.1016/j.exger.2013.07.001 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Dimitrijević, Mirjana
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/389
AB  - New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.
PB  - Wiley-Blackwell, Hoboken
T2  - Experimental Physiology
T1  - Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production
EP  - 678
IS  - 3
SP  - 665
VL  - 98
DO  - 10.1113/expphysiol.2012.070524
ER  - 

@article{
author = "Stanojević, Stanislava and Dimitrijević, Mirjana and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Leposavić, Gordana",
year = "2013",
abstract = "New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Experimental Physiology",
title = "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production",
pages = "678-665",
number = "3",
volume = "98",
doi = "10.1113/expphysiol.2012.070524"
}

Stanojević, S., Dimitrijević, M., Kuštrimović, N., Mitić, K., Vujić, V.,& Leposavić, G.. (2013). Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology
Wiley-Blackwell, Hoboken., 98(3), 665-678.
https://doi.org/10.1113/expphysiol.2012.070524

Stanojević S, Dimitrijević M, Kuštrimović N, Mitić K, Vujić V, Leposavić G. Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology. 2013;98(3):665-678.
doi:10.1113/expphysiol.2012.070524 .

Stanojević, Stanislava, Dimitrijević, Mirjana, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Leposavić, Gordana, "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production" in Experimental Physiology, 98, no. 3 (2013):665-678,
https://doi.org/10.1113/expphysiol.2012.070524 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/391
AB  - For many years, the central nervous system and the immune system were considered two autonomous entities. However, extensive research in the field of neuroimmunomodulation during the past decades has demonstrated the presence of different neuropeptides and their respective receptors in the immune cells. More importantly, it has provided evidence for the direct effects of neuropeptides on the immune cell functions. Neuropeptide Y (NPY) is generally considered the most abundant peptide in the central and peripheral nervous system. However, it is also distinguished by exhibiting pleiotropic functions in many other physiological systems, including the immune system. NPY affects the functions of the cells of the adaptive and innate immunity. In this respect, NPY is known to modulate immune cell trafficking, T helper cell differentiation, cytokine secretion, natural killer cell activity, phagocytosis and the production of reactive oxygen species. The specific Y receptors have been found in immune cells, and their expression is amplified upon immune stimulation. Different Y receptor subtypes may mediate an opposite effect of NPY on the particular function, thus underlining its regulatory role. Since the immune cells are capable of producing NPY upon appropriate stimulation, this peptide can regulate immune cell functions in an autocrine/paracrine manner. NPY also has important implications in several immune-mediated disorders, which affirms the clear need for further investigation of its role in either the mechanisms of the disease development or its possible therapeutic capacity. This review summarises the key points of NPY's mission throughout the immune system.
PB  - Springer Wien, Wien
T2  - Amino Acids
T1  - The intriguing mission of neuropeptide Y in the immune system
EP  - 53
IS  - 1
SP  - 41
VL  - 45
DO  - 10.1007/s00726-011-1185-7
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2013",
abstract = "For many years, the central nervous system and the immune system were considered two autonomous entities. However, extensive research in the field of neuroimmunomodulation during the past decades has demonstrated the presence of different neuropeptides and their respective receptors in the immune cells. More importantly, it has provided evidence for the direct effects of neuropeptides on the immune cell functions. Neuropeptide Y (NPY) is generally considered the most abundant peptide in the central and peripheral nervous system. However, it is also distinguished by exhibiting pleiotropic functions in many other physiological systems, including the immune system. NPY affects the functions of the cells of the adaptive and innate immunity. In this respect, NPY is known to modulate immune cell trafficking, T helper cell differentiation, cytokine secretion, natural killer cell activity, phagocytosis and the production of reactive oxygen species. The specific Y receptors have been found in immune cells, and their expression is amplified upon immune stimulation. Different Y receptor subtypes may mediate an opposite effect of NPY on the particular function, thus underlining its regulatory role. Since the immune cells are capable of producing NPY upon appropriate stimulation, this peptide can regulate immune cell functions in an autocrine/paracrine manner. NPY also has important implications in several immune-mediated disorders, which affirms the clear need for further investigation of its role in either the mechanisms of the disease development or its possible therapeutic capacity. This review summarises the key points of NPY's mission throughout the immune system.",
publisher = "Springer Wien, Wien",
journal = "Amino Acids",
title = "The intriguing mission of neuropeptide Y in the immune system",
pages = "53-41",
number = "1",
volume = "45",
doi = "10.1007/s00726-011-1185-7"
}

Dimitrijević, M.,& Stanojević, S.. (2013). The intriguing mission of neuropeptide Y in the immune system. in Amino Acids
Springer Wien, Wien., 45(1), 41-53.
https://doi.org/10.1007/s00726-011-1185-7

Dimitrijević M, Stanojević S. The intriguing mission of neuropeptide Y in the immune system. in Amino Acids. 2013;45(1):41-53.
doi:10.1007/s00726-011-1185-7 .

Dimitrijević, Mirjana, Stanojević, Stanislava, "The intriguing mission of neuropeptide Y in the immune system" in Amino Acids, 45, no. 1 (2013):41-53,
https://doi.org/10.1007/s00726-011-1185-7 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/386
AB  - We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Strain differences in the humoral immune response to commensal bacterial antigens in rats
EP  - 288
IS  - 3
SP  - 271
VL  - 60
DO  - 10.1556/AMicr.60.2013.3.4
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2013",
abstract = "We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Strain differences in the humoral immune response to commensal bacterial antigens in rats",
pages = "288-271",
number = "3",
volume = "60",
doi = "10.1556/AMicr.60.2013.3.4"
}

Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2013). Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 60(3), 271-288.
https://doi.org/10.1556/AMicr.60.2013.3.4

Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica. 2013;60(3):271-288.
doi:10.1556/AMicr.60.2013.3.4 .

Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences in the humoral immune response to commensal bacterial antigens in rats" in Acta Microbiologica et Immunologica Hungarica, 60, no. 3 (2013):271-288,
https://doi.org/10.1556/AMicr.60.2013.3.4 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/357
AB  - Neuropeptide Y (NPY) suppressed clinical experimental autoimmune encephalomyelitis (EAE) and reduced numbers of CD28+, CD11b+ and CD80+ cells among spinal cord infiltrating cells at the peak of disease in Dark Agouti rat strain. Suppression of EAE was accompanied by the reduced expression of costimulatory CD80 and CD86 molecules on ED1+ macrophages and OX62+ dendritic cells in draining lymph nodes during the inductive phase of EAE. An inhibitor of dipeptidyl peptidase 4, an enzyme which terminates the action of NPY on 11 receptor subtype, did not sustain the suppressive effect of NPY on the EAE development, suggesting involvement of Y2 and Y5 receptors. (C) 2012 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Neuroimmunology
T1  - NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions
EP  - 31
IS  - 1-2
SP  - 23
VL  - 245
DO  - 10.1016/j.jneuroim.2012.01.013
ER  - 

@article{
author = "Dimitrijević, Mirjana and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Stanojević, Stanislava",
year = "2012",
abstract = "Neuropeptide Y (NPY) suppressed clinical experimental autoimmune encephalomyelitis (EAE) and reduced numbers of CD28+, CD11b+ and CD80+ cells among spinal cord infiltrating cells at the peak of disease in Dark Agouti rat strain. Suppression of EAE was accompanied by the reduced expression of costimulatory CD80 and CD86 molecules on ED1+ macrophages and OX62+ dendritic cells in draining lymph nodes during the inductive phase of EAE. An inhibitor of dipeptidyl peptidase 4, an enzyme which terminates the action of NPY on 11 receptor subtype, did not sustain the suppressive effect of NPY on the EAE development, suggesting involvement of Y2 and Y5 receptors. (C) 2012 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions",
pages = "31-23",
number = "1-2",
volume = "245",
doi = "10.1016/j.jneuroim.2012.01.013"
}

Dimitrijević, M., Mitić, K., Kuštrimović, N., Vujić, V.,& Stanojević, S.. (2012). NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions. in Journal of Neuroimmunology
Elsevier, Amsterdam., 245(1-2), 23-31.
https://doi.org/10.1016/j.jneuroim.2012.01.013

Dimitrijević M, Mitić K, Kuštrimović N, Vujić V, Stanojević S. NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions. in Journal of Neuroimmunology. 2012;245(1-2):23-31.
doi:10.1016/j.jneuroim.2012.01.013 .

Dimitrijević, Mirjana, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Stanojević, Stanislava, "NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions" in Journal of Neuroimmunology, 245, no. 1-2 (2012):23-31,
https://doi.org/10.1016/j.jneuroim.2012.01.013 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Leposavić, Gordana
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/359
AB  - Much evidence has identified a direct anatomical and functional link between the brain and the immune system, with glucocorticoids (GCs), catecholamines (CAs), and neuropeptide Y (NPY) as its end-point mediators. This suggests the important role of these mediators in immune system homeostasis and the pathogenesis of inflammatory autoimmune diseases. However, although it is clear that these mediators can modulate lymphocyte maturation and the activity of distinct immune cell types, their putative role in the pathogenesis of autoimmune disease is not yet completely understood. We have contributed to this field by discovering the influence of CAs and GCs on fine-tuning thymocyte negative selection and, in particular, by pointing to the putative CA-mediated mechanisms underlying this influence. Furthermore, we have shown that CAs are implicated in the regulation of regulatory T-cell development in the thymus. Moreover, our investigations related to macrophage biology emphasize the complex interaction between GCs, CAs and NPY in the modulation of macrophage functions and their putative significance for the pathogenesis of autoimmune inflammatory diseases.
PB  - Humana Press Inc, Totowa
T2  - Immunologic Research
T1  - End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology
EP  - 80
IS  - 1-2
SP  - 64
VL  - 52
DO  - 10.1007/s12026-012-8275-9
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kuštrimović, Nataša and Leposavić, Gordana",
year = "2012",
abstract = "Much evidence has identified a direct anatomical and functional link between the brain and the immune system, with glucocorticoids (GCs), catecholamines (CAs), and neuropeptide Y (NPY) as its end-point mediators. This suggests the important role of these mediators in immune system homeostasis and the pathogenesis of inflammatory autoimmune diseases. However, although it is clear that these mediators can modulate lymphocyte maturation and the activity of distinct immune cell types, their putative role in the pathogenesis of autoimmune disease is not yet completely understood. We have contributed to this field by discovering the influence of CAs and GCs on fine-tuning thymocyte negative selection and, in particular, by pointing to the putative CA-mediated mechanisms underlying this influence. Furthermore, we have shown that CAs are implicated in the regulation of regulatory T-cell development in the thymus. Moreover, our investigations related to macrophage biology emphasize the complex interaction between GCs, CAs and NPY in the modulation of macrophage functions and their putative significance for the pathogenesis of autoimmune inflammatory diseases.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology",
pages = "80-64",
number = "1-2",
volume = "52",
doi = "10.1007/s12026-012-8275-9"
}

Dimitrijević, M., Stanojević, S., Kuštrimović, N.,& Leposavić, G.. (2012). End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology. in Immunologic Research
Humana Press Inc, Totowa., 52(1-2), 64-80.
https://doi.org/10.1007/s12026-012-8275-9

Dimitrijević M, Stanojević S, Kuštrimović N, Leposavić G. End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology. in Immunologic Research. 2012;52(1-2):64-80.
doi:10.1007/s12026-012-8275-9 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kuštrimović, Nataša, Leposavić, Gordana, "End-point effector stress mediators in neuroimmune interactions: their role in immune system homeostasis and autoimmune pathology" in Immunologic Research, 52, no. 1-2 (2012):64-80,
https://doi.org/10.1007/s12026-012-8275-9 . .

TY  - CHAP
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/352
AB  - Neuropeptide Y (NPY) is a peptide consisted of 36 amino acids. It is designated by a capital letter Y due to the presence of many tyrosine residues which are abbreviated by the letter Y in the single letter amino acid code. NPY is one of the most evolutionary conserved peptides, originally isolated from pig brain (Tatemoto et al. 1982; Larhammar 1996). Due to a very wide tissue distribution of NPY and its significance for the human and animal physiology, peptides with high structural homology to NPY, e.g., peptide YY (PYY), pancreatic polypeptide (PP) and their truncated forms NPY2-36, NPY3-36 and PYY3-36, sharing amino acid backbone that forms a hair-pin turn called the PP-fold (Fuhlendorff et al. 1990), are now specified as members of NPY family. NPY was previously considered a companion and amplifier of norepinephrine activity. It is now known that NPY could be stored alone in small vesicles of sympathetic nerves, and in combination with catecholamines in large vesicles (Fried et al. 1985). Although NPY is preferentially released under conditions of high frequency nerve stimulation, the endogenous NPY modulates the effects of norepinephrine at both high and low levels of sympathetic nerve activity (Han et al. 1998).
PB  - Springer-Verlag Wien
T2  - Nerve-Driven Immunity: Neurotransmitters and Neuropeptides in the Immune System
T1  - Neuropeptide Y: The story, the players, the outcomes
EP  - 256
SP  - 227
DO  - 10.1007/978-3-7091-0888-8_8
ER  - 

@inbook{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2012",
abstract = "Neuropeptide Y (NPY) is a peptide consisted of 36 amino acids. It is designated by a capital letter Y due to the presence of many tyrosine residues which are abbreviated by the letter Y in the single letter amino acid code. NPY is one of the most evolutionary conserved peptides, originally isolated from pig brain (Tatemoto et al. 1982; Larhammar 1996). Due to a very wide tissue distribution of NPY and its significance for the human and animal physiology, peptides with high structural homology to NPY, e.g., peptide YY (PYY), pancreatic polypeptide (PP) and their truncated forms NPY2-36, NPY3-36 and PYY3-36, sharing amino acid backbone that forms a hair-pin turn called the PP-fold (Fuhlendorff et al. 1990), are now specified as members of NPY family. NPY was previously considered a companion and amplifier of norepinephrine activity. It is now known that NPY could be stored alone in small vesicles of sympathetic nerves, and in combination with catecholamines in large vesicles (Fried et al. 1985). Although NPY is preferentially released under conditions of high frequency nerve stimulation, the endogenous NPY modulates the effects of norepinephrine at both high and low levels of sympathetic nerve activity (Han et al. 1998).",
publisher = "Springer-Verlag Wien",
journal = "Nerve-Driven Immunity: Neurotransmitters and Neuropeptides in the Immune System",
booktitle = "Neuropeptide Y: The story, the players, the outcomes",
pages = "256-227",
doi = "10.1007/978-3-7091-0888-8_8"
}

Dimitrijević, M.,& Stanojević, S.. (2012). Neuropeptide Y: The story, the players, the outcomes. in Nerve-Driven Immunity: Neurotransmitters and Neuropeptides in the Immune System
Springer-Verlag Wien., 227-256.
https://doi.org/10.1007/978-3-7091-0888-8_8

Dimitrijević M, Stanojević S. Neuropeptide Y: The story, the players, the outcomes. in Nerve-Driven Immunity: Neurotransmitters and Neuropeptides in the Immune System. 2012;:227-256.
doi:10.1007/978-3-7091-0888-8_8 .

Dimitrijević, Mirjana, Stanojević, Stanislava, "Neuropeptide Y: The story, the players, the outcomes" in Nerve-Driven Immunity: Neurotransmitters and Neuropeptides in the Immune System (2012):227-256,
https://doi.org/10.1007/978-3-7091-0888-8_8 . .

TY  - CHAP
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/343
PB  - Springer, Wien-New York
T2  - Nerve-Driven Immunity- Neurotransmitters and Neuropeptides in the Immune System
T1  - Neuropeptide Y: The story, the players, the outcomes
EP  - 255
SP  - 277
UR  - https://hdl.handle.net/21.15107/rcub_intor_343
ER  - 

@inbook{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2012",
publisher = "Springer, Wien-New York",
journal = "Nerve-Driven Immunity- Neurotransmitters and Neuropeptides in the Immune System",
booktitle = "Neuropeptide Y: The story, the players, the outcomes",
pages = "255-277",
url = "https://hdl.handle.net/21.15107/rcub_intor_343"
}

Dimitrijević, M.,& Stanojević, S.. (2012). Neuropeptide Y: The story, the players, the outcomes. in Nerve-Driven Immunity- Neurotransmitters and Neuropeptides in the Immune System
Springer, Wien-New York., 277-255.
https://hdl.handle.net/21.15107/rcub_intor_343

Dimitrijević M, Stanojević S. Neuropeptide Y: The story, the players, the outcomes. in Nerve-Driven Immunity- Neurotransmitters and Neuropeptides in the Immune System. 2012;:277-255.
https://hdl.handle.net/21.15107/rcub_intor_343 .

Dimitrijević, Mirjana, Stanojević, Stanislava, "Neuropeptide Y: The story, the players, the outcomes" in Nerve-Driven Immunity- Neurotransmitters and Neuropeptides in the Immune System (2012):277-255,
https://hdl.handle.net/21.15107/rcub_intor_343 .

TY  - CONF
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/325
PB  - Birkhauser Verlag Ag, Basel
C3  - Inflammation Research
T1  - Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells
EP  - 87
SP  - 87
VL  - 60
UR  - https://hdl.handle.net/21.15107/rcub_intor_325
ER  - 

@conference{
author = "Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2011",
publisher = "Birkhauser Verlag Ag, Basel",
journal = "Inflammation Research",
title = "Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells",
pages = "87-87",
volume = "60",
url = "https://hdl.handle.net/21.15107/rcub_intor_325"
}

Kuštrimović, N., Mitić, K., Vujić, V., Dimitrijević, M.,& Stanojević, S.. (2011). Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells. in Inflammation Research
Birkhauser Verlag Ag, Basel., 60, 87-87.
https://hdl.handle.net/21.15107/rcub_intor_325

Kuštrimović N, Mitić K, Vujić V, Dimitrijević M, Stanojević S. Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells. in Inflammation Research. 2011;60:87-87.
https://hdl.handle.net/21.15107/rcub_intor_325 .

Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, Stanojević, Stanislava, "Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells" in Inflammation Research, 60 (2011):87-87,
https://hdl.handle.net/21.15107/rcub_intor_325 .

TY  - JOUR
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/326
AB  - The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection.
AB  - Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors
T1  - Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora
EP  - 132
IS  - 2-3
SP  - 119
VL  - 61
DO  - 10.2298/AVB1103119K
ER  - 

@article{
author = "Kuštrimović, Nataša and Mitić, Katarina and Dimitrijević, Mirjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava",
year = "2011",
abstract = "The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection., Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors, Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora",
pages = "132-119",
number = "2-3",
volume = "61",
doi = "10.2298/AVB1103119K"
}

Kuštrimović, N., Mitić, K., Dimitrijević, M., Vujić, V., Kovačević-Jovanović, V., Miletić, T.,& Stanojević, S.. (2011). Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 119-132.
https://doi.org/10.2298/AVB1103119K

Kuštrimović N, Mitić K, Dimitrijević M, Vujić V, Kovačević-Jovanović V, Miletić T, Stanojević S. Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd. 2011;61(2-3):119-132.
doi:10.2298/AVB1103119K .

Kuštrimović, Nataša, Mitić, Katarina, Dimitrijević, Mirjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors" in Acta veterinaria - Beograd, 61, no. 2-3 (2011):119-132,
https://doi.org/10.2298/AVB1103119K . .

TY  - JOUR
AU  - Mitić, Katarina
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/327
AB  - Neuropeptide Y (NPY) has been reported to be a potent anti-inflammatory peptide with ability to directly modulate activity of granulocytes and macrophages. The present study aimed to correlate the effects of NPY in vivo on lipopolysaccharide-induced air-pouch exudates cells and in vitro on peripheral blood leukocytes functions. The role of different Y receptors was examined using NPY-related peptides and antagonists with diverse subtype specificity and selectivity for Y receptors. Y1, Y2 and Y5 receptors were detected on air-pouch exudates cells (flow cytometry) and peripheral blood granulocytes (immunocito-chemistry). NPY in vivo reduced inflammatory cells accumulation into the air pouch, and decreased their adherence and phagocytic capacity via Y2/Y5 and Y1/Y2 receptors, respectively. Quite the opposite, NPY in vitro potentiated adhesiveness and phagocytosis of peripheral blood granulocytes and monocytes by activating Y1 receptor. The differences between in vivo and in vitro effects of NPY on rat inflammatory cells functions are mostly due to dipeptidyl peptidase 4 activity. In addition, suppressive effect of NPY in vivo is highly dependent on the local microenvironment, peptide truncation and specific Y receptors interplay. (C) 2011 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors
EP  - 1633
IS  - 8
SP  - 1626
VL  - 32
DO  - 10.1016/j.peptides.2011.06.007
ER  - 

@article{
author = "Mitić, Katarina and Stanojević, Stanislava and Kuštrimović, Nataša and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2011",
abstract = "Neuropeptide Y (NPY) has been reported to be a potent anti-inflammatory peptide with ability to directly modulate activity of granulocytes and macrophages. The present study aimed to correlate the effects of NPY in vivo on lipopolysaccharide-induced air-pouch exudates cells and in vitro on peripheral blood leukocytes functions. The role of different Y receptors was examined using NPY-related peptides and antagonists with diverse subtype specificity and selectivity for Y receptors. Y1, Y2 and Y5 receptors were detected on air-pouch exudates cells (flow cytometry) and peripheral blood granulocytes (immunocito-chemistry). NPY in vivo reduced inflammatory cells accumulation into the air pouch, and decreased their adherence and phagocytic capacity via Y2/Y5 and Y1/Y2 receptors, respectively. Quite the opposite, NPY in vitro potentiated adhesiveness and phagocytosis of peripheral blood granulocytes and monocytes by activating Y1 receptor. The differences between in vivo and in vitro effects of NPY on rat inflammatory cells functions are mostly due to dipeptidyl peptidase 4 activity. In addition, suppressive effect of NPY in vivo is highly dependent on the local microenvironment, peptide truncation and specific Y receptors interplay. (C) 2011 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors",
pages = "1633-1626",
number = "8",
volume = "32",
doi = "10.1016/j.peptides.2011.06.007"
}

Mitić, K., Stanojević, S., Kuštrimović, N., Vujić, V.,& Dimitrijević, M.. (2011). Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors. in Peptides
Elsevier Science Inc, New York., 32(8), 1626-1633.
https://doi.org/10.1016/j.peptides.2011.06.007

Mitić K, Stanojević S, Kuštrimović N, Vujić V, Dimitrijević M. Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors. in Peptides. 2011;32(8):1626-1633.
doi:10.1016/j.peptides.2011.06.007 .

Mitić, Katarina, Stanojević, Stanislava, Kuštrimović, Nataša, Vujić, Vesna, Dimitrijević, Mirjana, "Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors" in Peptides, 32, no. 8 (2011):1626-1633,
https://doi.org/10.1016/j.peptides.2011.06.007 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/298
AB  - It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Regulatory Peptides
T1  - Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity
EP  - 109
IS  - 1-3
SP  - 100
VL  - 159
DO  - 10.1016/j.regpep.2009.11.002
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2010",
abstract = "It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity",
pages = "109-100",
number = "1-3",
volume = "159",
doi = "10.1016/j.regpep.2009.11.002"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2010). Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 159(1-3), 100-109.
https://doi.org/10.1016/j.regpep.2009.11.002

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides. 2010;159(1-3):100-109.
doi:10.1016/j.regpep.2009.11.002 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity" in Regulatory Peptides, 159, no. 1-3 (2010):100-109,
https://doi.org/10.1016/j.regpep.2009.11.002 . .

TY  - JOUR
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Kuštrimović, Nataša
AU  - Kosec, Duško
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/311
AB  - We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity.
AB  - Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction
T1  - Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).
EP  - 121
IS  - 2-3
SP  - 105
VL  - 60
DO  - 10.2298/AVB1003105M
ER  - 

@article{
author = "Mitić, Katarina and Miletić, Tatjana and Kovačević-Jovanović, Vesna and Kuštrimović, Nataša and Kosec, Duško and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2010",
abstract = "We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity., Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction, Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).",
pages = "121-105",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003105M"
}

Mitić, K., Miletić, T., Kovačević-Jovanović, V., Kuštrimović, N., Kosec, D., Dimitrijević, M.,& Stanojević, S.. (2010). Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 105-121.
https://doi.org/10.2298/AVB1003105M

Mitić K, Miletić T, Kovačević-Jovanović V, Kuštrimović N, Kosec D, Dimitrijević M, Stanojević S. Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd. 2010;60(2-3):105-121.
doi:10.2298/AVB1003105M .

Mitić, Katarina, Miletić, Tatjana, Kovačević-Jovanović, Vesna, Kuštrimović, Nataša, Kosec, Duško, Dimitrijević, Mirjana, Stanojević, Stanislava, "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction" in Acta veterinaria - Beograd, 60, no. 2-3 (2010):105-121,
https://doi.org/10.2298/AVB1003105M . .