TY  - JOUR
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Dragačević, Luka
AU  - Vujić, Vesna
AU  - Lukić, Ivana
AU  - Stanojević, Stanislava
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/693
AB  - BCG vaccination induces a memory-like response in innate immune cells known as
trained immunity. In this study, we investigated the modification of innate immune
cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with
thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with
a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were
examined for 7 days after TG injection by flow cytometry, and for NO production and
peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage
cells as following: increased in vitro LPS and BCG stimulated NO production from total
cells compared to adherent cells (day 1); increased cell number (days 3 and 5);
increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and
eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on
CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase
activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On
day 7, cells from both experimental groups showed no production of NO in response to
in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the
acute phase of sterile inflammation, which may lead to the later observed phenotypic
and functional changes that could be seen as accelerated resolution of inflammation
and possibly point to trained immune response.
PB  - Wiley
T1  - BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?
EP  - 127
IS  - S2
SP  - 127
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_intor_693
ER  - 

@article{
author = "Ćuruvija, Ivana and Blagojević, Veljko and Dragačević, Luka and Vujić, Vesna and Lukić, Ivana and Stanojević, Stanislava",
year = "2022",
abstract = "BCG vaccination induces a memory-like response in innate immune cells known as
trained immunity. In this study, we investigated the modification of innate immune
cells by BCG vaccination in acute peritoneal inflammation. We induced peritonitis with
thioglycollate (TG) in young Albino Oxford male rats which were immunised s.c. with
a BCG vaccine (BCG group) or saline (control group) 7 days prior. Peritoneal cells were
examined for 7 days after TG injection by flow cytometry, and for NO production and
peroxidase activity. Prior in vivo BCG priming altered TG-elicited peritoneal lavage
cells as following: increased in vitro LPS and BCG stimulated NO production from total
cells compared to adherent cells (day 1); increased cell number (days 3 and 5);
increased percentage of inflammatory monocytes (SSCmidCD43lowCD11bmid) and
eosinophils (SSCHihiS48+CD43hi), and a higher level of surface CD11b expression on
CD163+ macrophages (day 5); increased in vitro LPS and BCG stimulated peroxidase
activity (days 5 and 7); and increased percentage of CD163+MHCII+ cells (day 7). On
day 7, cells from both experimental groups showed no production of NO in response to
in vitro stimulation. We conclude that BCG vaccination had a substantial effect on the
acute phase of sterile inflammation, which may lead to the later observed phenotypic
and functional changes that could be seen as accelerated resolution of inflammation
and possibly point to trained immune response.",
publisher = "Wiley",
title = "BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?",
pages = "127-127",
number = "S2",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_intor_693"
}

Ćuruvija, I., Blagojević, V., Dragačević, L., Vujić, V., Lukić, I.,& Stanojević, S.. (2022). BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?. 
Wiley., 52(S2), 127-127.
https://hdl.handle.net/21.15107/rcub_intor_693

Ćuruvija I, Blagojević V, Dragačević L, Vujić V, Lukić I, Stanojević S. BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?. 2022;52(S2):127-127.
https://hdl.handle.net/21.15107/rcub_intor_693 .

Ćuruvija, Ivana, Blagojević, Veljko, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, "BCG vaccination induced alterations of thioglycollate-elicited peritoneal phagocytes: a case of trained immunity?", 52, no. S2 (2022):127-127,
https://hdl.handle.net/21.15107/rcub_intor_693 .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Dragačević, Luka
AU  - Vujić, Vesna
AU  - Lukić, Ivana
AU  - Stanojević, Stanislava
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/694
AB  - Inflammation is a redistribution of immune cells, providing a more efficient elimination of the inflammatory offense. However, it is not limited to local microenvironment. In this study, the interaction of the effect of BCG priming and peritoneal inflammation on the remote inflammatory milieu of infected lung was investigated. Young male AO rats infected with Mycoplasma spp. were s.c. injected with BCG (3x105 CFU) or saline, and 7 days later received an i.p. injection of 7ml of thioglycollate (TG) or saline. Up to 7 days after TG injection, a broncho-alveolar lavage (BAL) was performed, and cells were analysed for their surface marker expression and NO production. Infected rats had a high percentage of HIS48HiCD11bHi neutrophils. BCG priming didn’t alter BAL cells phenotype, while TG injection increased the proportion of MHCII+CD11blow activated alveolar macrophages (aAMFs) on day 7. However, the BCG+TG group showed significant changes – percentage of HIS48HiCD11bHi neutrophils decreased from day 3, the share of aAMFs increased from day 5 and the share of MHCII+CD11b-AMFs increased on days 3-5. However, the percentage of B220+FSClow B lymphocytes were increased from day 1. Production of NO from BAL fluid cells was low in all groups. We conclude that BCG vaccination likely increased the number of circulating B lymphocytes, while TG-induced peritoneal inflammation potentially prevented their entry into the peritoneal cavity, forcing them into permissive tissues, such as lungs.
PB  - Wiley
T1  - Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation
EP  - 55
IS  - S2
SP  - 55
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_intor_694
ER  - 

@conference{
author = "Blagojević, Veljko and Ćuruvija, Ivana and Dragačević, Luka and Vujić, Vesna and Lukić, Ivana and Stanojević, Stanislava",
year = "2022",
abstract = "Inflammation is a redistribution of immune cells, providing a more efficient elimination of the inflammatory offense. However, it is not limited to local microenvironment. In this study, the interaction of the effect of BCG priming and peritoneal inflammation on the remote inflammatory milieu of infected lung was investigated. Young male AO rats infected with Mycoplasma spp. were s.c. injected with BCG (3x105 CFU) or saline, and 7 days later received an i.p. injection of 7ml of thioglycollate (TG) or saline. Up to 7 days after TG injection, a broncho-alveolar lavage (BAL) was performed, and cells were analysed for their surface marker expression and NO production. Infected rats had a high percentage of HIS48HiCD11bHi neutrophils. BCG priming didn’t alter BAL cells phenotype, while TG injection increased the proportion of MHCII+CD11blow activated alveolar macrophages (aAMFs) on day 7. However, the BCG+TG group showed significant changes – percentage of HIS48HiCD11bHi neutrophils decreased from day 3, the share of aAMFs increased from day 5 and the share of MHCII+CD11b-AMFs increased on days 3-5. However, the percentage of B220+FSClow B lymphocytes were increased from day 1. Production of NO from BAL fluid cells was low in all groups. We conclude that BCG vaccination likely increased the number of circulating B lymphocytes, while TG-induced peritoneal inflammation potentially prevented their entry into the peritoneal cavity, forcing them into permissive tissues, such as lungs.",
publisher = "Wiley",
title = "Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation",
pages = "55-55",
number = "S2",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_intor_694"
}

Blagojević, V., Ćuruvija, I., Dragačević, L., Vujić, V., Lukić, I.,& Stanojević, S.. (2022). Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation. 
Wiley., 52(S2), 55-55.
https://hdl.handle.net/21.15107/rcub_intor_694

Blagojević V, Ćuruvija I, Dragačević L, Vujić V, Lukić I, Stanojević S. Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation. 2022;52(S2):55-55.
https://hdl.handle.net/21.15107/rcub_intor_694 .

Blagojević, Veljko, Ćuruvija, Ivana, Dragačević, Luka, Vujić, Vesna, Lukić, Ivana, Stanojević, Stanislava, "Modifying Mycoplasma-infected lung immune cells through an intriguing interplay of BCG priming and peritoneal inflammation", 52, no. S2 (2022):55-55,
https://hdl.handle.net/21.15107/rcub_intor_694 .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Veljović, Katarina
AU  - Soković Bajić, Svetlana
AU  - Stanojević, Stanislava
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/667
AB  - We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from 
day 4  to day 30). Feces were collected  for microbial analysis. Colitis was induced at day 85. Seven days later  rats were graded  for histological damage in colon, and samples of 
mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic 
treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well 
as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells, 
attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rats. Our findings reveal that effects of probiotic treatment are sex‐specific to an 
extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood. 
The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to 
be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and 
severity of colitis symptoms in untreated rats.
PB  - Wiley
C3  - European Journal of Immunology
T1  - Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats
EP  - 311
SP  - 311
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_intor_667
ER  - 

@conference{
author = "Blagojević, Veljko and Vujić, Vesna and Ćuruvija, Ivana and Veljović, Katarina and Soković Bajić, Svetlana and Stanojević, Stanislava",
year = "2021",
abstract = "We tested the effects of early‐life probiotic treatment on the induction of colitis in female and male adult rats. Rat pups were fed an aqueous solution of Lactobacillus rhamnosus (from 
day 4  to day 30). Feces were collected  for microbial analysis. Colitis was induced at day 85. Seven days later  rats were graded  for histological damage in colon, and samples of 
mesenteric lymph node (MLN) and peritoneal exudate cells were analyzed. Female rats developed slightly less severe symptoms of colitis than males, whereas early‐life probiotic 
treatment had a more pronounced effect on males in nearly every analyzed parameter. Namely, it increased fecal bacterial diversity and ameliorated colon tissue damage, as well 
as increased percentage of resident peritoneal macrophages (CD163+), decreased peritoneal monocyte (HIS48+CD43+) influx, reduced production of IFNγ and IL10 by MLN cells, 
attenuated NO production in stimulated peritoneal macrophages and unstimulated MLN cells of male rats. Our findings reveal that effects of probiotic treatment are sex‐specific to an 
extent. While microbial diversity was impacted by probiotic treatment in both sexes at an early age, the effect was more pronounced in young males, and it lasted to their adulthood. 
The change in microbial diversity correlated with improved outcome of TNBS‐induced colitis, confirming the importance of microbiota for local inflammatory processes. It remains to 
be elucidated whether the sex differences in the effect of probiotic treatment on development of colitis may be a consequence of sex differences in early‐life microbial diversity and 
severity of colitis symptoms in untreated rats.",
publisher = "Wiley",
journal = "European Journal of Immunology",
title = "Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats",
pages = "311-311",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_intor_667"
}

Blagojević, V., Vujić, V., Ćuruvija, I., Veljović, K., Soković Bajić, S.,& Stanojević, S.. (2021). Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats. in European Journal of Immunology
Wiley., 51, 311-311.
https://hdl.handle.net/21.15107/rcub_intor_667

Blagojević V, Vujić V, Ćuruvija I, Veljović K, Soković Bajić S, Stanojević S. Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats. in European Journal of Immunology. 2021;51:311-311.
https://hdl.handle.net/21.15107/rcub_intor_667 .

Blagojević, Veljko, Vujić, Vesna, Ćuruvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Stanojević, Stanislava, "Sex differences in the effects of early‐life probiotic treatment on TNBS‐induced colitis in rats" in European Journal of Immunology, 51 (2021):311-311,
https://hdl.handle.net/21.15107/rcub_intor_667 .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujić, Vesna
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/611
AB  - Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.
PB  - Springer Nature
T2  - Inflammation
T1  - Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity
DO  - 10.1007/s10753-021-01513-z
ER  - 

@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujić, Vesna",
year = "2021",
abstract = "Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.",
publisher = "Springer Nature",
journal = "Inflammation",
title = "Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity",
doi = "10.1007/s10753-021-01513-z"
}

Stanojević, S., Blagojević, V., Ćuruvija, I.,& Vujić, V.. (2021). Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. in Inflammation
Springer Nature..
https://doi.org/10.1007/s10753-021-01513-z

Stanojević S, Blagojević V, Ćuruvija I, Vujić V. Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. in Inflammation. 2021;.
doi:10.1007/s10753-021-01513-z .

Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujić, Vesna, "Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity" in Inflammation (2021),
https://doi.org/10.1007/s10753-021-01513-z . .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Ćuruvija, Ivana
AU  - Prijić, Ivana
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/665
AB  - Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"
PB  - University of Belgrade; Immunological Society of Serbia
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia
T1  - Potential impact of early-life probiotic supplementation on peritoneal macrophage function
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_intor_665
ER  - 

@conference{
author = "Blagojević, Veljko and Petrović, Raisa and Ćuruvija, Ivana and Prijić, Ivana and Vujić, Vesna and Stanojević, Stanislava",
year = "2019",
abstract = "Clinical and animal trials show that early life probiotic consumption provides health benefits in adult life by modulating the immune response. We tested the effects of early life oral consumption of the probiotic Lactobacillus rhamnosus on the function and phenotype of rat peritoneal cavity cells in a model of induced colitis. For the first month of their lives, rats were either fed with an aqueous probiotic bacteria suspension (LB group) or tap water (control group). When the rats grew to 3 months old, we studied the response of their peritoneal macrophages to autologous fecal bacteria stimulation in vitro, both before and after colitis induction (TNBS 40mg/kg of body mass in 50% ethanol). Compared to the controls, the peritoneal cavity cells of the LB group produced less nitric oxide (NO) and had an increased proportion of CD163+ cells. The rats in the LB group have shown milder symptoms of colitis (shorter length of colon under necrosis, less severe submucosal infiltration, lesser degree of colonic wall thickening), along with a diminished increase of peritoneal proinflammatory CCR7+ cells and blunted NO production in response to stimulation by autologous fecal bacteria. Our results may indicate that early oral probiotic administration attenuates macrophage responses to fecal bacteria, which are the primary cause of tissue inflammation and necrosis in chemically induced colitis models, and that this attenuation may be involved in improving the health of colitic rats.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia",
title = "Potential impact of early-life probiotic supplementation on peritoneal macrophage function",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_intor_665"
}

Blagojević, V., Petrović, R., Ćuruvija, I., Prijić, I., Vujić, V.,& Stanojević, S.. (2019). Potential impact of early-life probiotic supplementation on peritoneal macrophage function. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"., 34.
https://hdl.handle.net/21.15107/rcub_intor_665

Blagojević V, Petrović R, Ćuruvija I, Prijić I, Vujić V, Stanojević S. Potential impact of early-life probiotic supplementation on peritoneal macrophage function. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. 2019;:34.
https://hdl.handle.net/21.15107/rcub_intor_665 .

Blagojević, Veljko, Petrović, Raisa, Ćuruvija, Ivana, Prijić, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Potential impact of early-life probiotic supplementation on peritoneal macrophage function" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia (2019):34,
https://hdl.handle.net/21.15107/rcub_intor_665 .

TY  - CONF
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Prijić, Ivana
AU  - Vujić, Vesna
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/664
AB  - Aging differently affects the expression of estrogen receptors alpha and beta
(ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of
male and female rats. We explored the involvement of ERα and ERβ in the in
vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol
(which stimulates both receptors) or genistein (which is predominantly an ERβ
agonist) on inflammatory cytokine secretion from young (3 months old) and
middle-aged (16 months old) female and male AO rats. Aging diminished the
proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages
from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via
ERα, and suppressed it in cells from young females via ERβ. Genistein-induced
decrease of IL-1β in macrophages from all experimental groups was probably
mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all
experimental groups via ERα while genistein diminished it in all females’ and
in middle-aged male rats’ macrophages by activating ERβ. However, genistein
increased IL-6 secretion from macrophages of young male rats via ERα.
Although 17β-estradiol and genistein stimulated secretion of macrophage
inflammatory cytokines via ERα and suppressed it probably via ERβ, their
modulatory actions were determined by aging-induced changes in macrophage
ERs expression and possible ERα / ERβ interactions (Supported by Ministry of
Education, Science and Technological development, Republic of Serbia, Grant
No 175050).
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"
PB  - University of Belgrade; Immunological Society of Serbia
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.
T1  - 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner
EP  - 132
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_intor_664
ER  - 

@conference{
author = "Ćuruvija, Ivana and Stanojević, Stanislava and Blagojević, Veljko and Petrović, Raisa and Prijić, Ivana and Vujić, Vesna",
year = "2019",
abstract = "Aging differently affects the expression of estrogen receptors alpha and beta
(ERα and ERβ) and Toll-like receptors (TLR4) on peritoneal cavity cells of
male and female rats. We explored the involvement of ERα and ERβ in the in
vitro treatment of LPS-stimulated peritoneal macrophages with 17β-estradiol
(which stimulates both receptors) or genistein (which is predominantly an ERβ
agonist) on inflammatory cytokine secretion from young (3 months old) and
middle-aged (16 months old) female and male AO rats. Aging diminished the
proportion of TLR4+ cells and secretion of IL-1β and IL-6 in macrophages
from female rats while the effect on male rat macrophages was opposite. 17βestradiol increased IL-1β secretion by middle-aged females’ macrophages via
ERα, and suppressed it in cells from young females via ERβ. Genistein-induced
decrease of IL-1β in macrophages from all experimental groups was probably
mediated by ERβ. 17β-estradiol augmented IL-6 secretion by cells from all
experimental groups via ERα while genistein diminished it in all females’ and
in middle-aged male rats’ macrophages by activating ERβ. However, genistein
increased IL-6 secretion from macrophages of young male rats via ERα.
Although 17β-estradiol and genistein stimulated secretion of macrophage
inflammatory cytokines via ERα and suppressed it probably via ERβ, their
modulatory actions were determined by aging-induced changes in macrophage
ERs expression and possible ERα / ERβ interactions (Supported by Ministry of
Education, Science and Technological development, Republic of Serbia, Grant
No 175050).",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade; Immunological Society of Serbia",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.",
title = "17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner",
pages = "132-132",
url = "https://hdl.handle.net/21.15107/rcub_intor_664"
}

Ćuruvija, I., Stanojević, S., Blagojević, V., Petrović, R., Prijić, I.,& Vujić, V.. (2019). 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.
Belgrade: Institute for Biological Research "Siniša Stanković"., 132-132.
https://hdl.handle.net/21.15107/rcub_intor_664

Ćuruvija I, Stanojević S, Blagojević V, Petrović R, Prijić I, Vujić V. 17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia.. 2019;:132-132.
https://hdl.handle.net/21.15107/rcub_intor_664 .

Ćuruvija, Ivana, Stanojević, Stanislava, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, "17β-Estradiol and genistein affect macrophage inflammatory cytokine production during aging in sex-specific manner" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book: 2019 Dec 6-8; Belgrade, Serbia. (2019):132-132,
https://hdl.handle.net/21.15107/rcub_intor_664 .

TY  - JOUR
AU  - Blagojević, Veljko
AU  - Kovačević-Jovanović, Vesna
AU  - Ćuruvija, Ivana
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/512
AB  - Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?
EP  - 157
SP  - 147
VL  - 197
DO  - 10.1016/j.lfs.2018.02.011
ER  - 

@article{
author = "Blagojević, Veljko and Kovačević-Jovanović, Vesna and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, Ivana and Vujić, Vesna and Stanojević, Stanislava",
year = "2018",
abstract = "Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?",
pages = "157-147",
volume = "197",
doi = "10.1016/j.lfs.2018.02.011"
}

Blagojević, V., Kovačević-Jovanović, V., Ćuruvija, I., Petrović, R., Vujnović, I., Vujić, V.,& Stanojević, S.. (2018). Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 197, 147-157.
https://doi.org/10.1016/j.lfs.2018.02.011

Blagojević V, Kovačević-Jovanović V, Ćuruvija I, Petrović R, Vujnović I, Vujić V, Stanojević S. Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences. 2018;197:147-157.
doi:10.1016/j.lfs.2018.02.011 .

Blagojević, Veljko, Kovačević-Jovanović, Vesna, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?" in Life Sciences, 197 (2018):147-157,
https://doi.org/10.1016/j.lfs.2018.02.011 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Prijić, Ivana
AU  - Vujić, Vesna
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/511
AB  - The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute to better understanding of the complex role(s) that E2 may play in innate immunity during aging, and that are dependent of sex.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats
EP  - 94
SP  - 86
VL  - 113
DO  - 10.1016/j.exger.2018.09.024
ER  - 

@article{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Petrović, Raisa and Prijić, Ivana and Vujić, Vesna",
year = "2018",
abstract = "The systemic and extra- gonadal levels of 17 beta-estradiol (E2) change during aging, and affect the expression of estrogen receptors (ERs) in the immune cells of both females and males. The age-related cessation of ovarian function in females, as well as the tissue-specific expression of enzyme aromatase (estrogen synthase which significantly rises with the advancing age) in both males and females, both determine the concentration of E2 to which immune cells may be exposed. The present study was set up to investigate the direct influence of E2 in vitro on the secretory profile of peritoneal macrophages from young and naturally menopausal female rats, and from young and middle-aged male rats. The involvement of receptor(s) responsible for mediating the effects of E2 in vitro was examined by use of antagonists specific for ERa or ER beta. Whereas in macrophages from young female rats E2 treatment diminished interleukin (IL)-1 beta secretion, it increased it in young males, and the middleaged females. The in vitro E2 treatment increased tumor necrosis factor (TNF)-alpha release by macrophages from young rats of both sexes, while it increased macrophage IL-6 release independently of both sex and age. At the same time, E2 decreased hydrogen peroxide (H2O2) production in macrophages from females, and increased it in male rats of both ages, whereas it diminished nitric oxide (NO) release in all experimental groups. Inspite of the sex-and age-specific effects of E2 on macrophage urea release, E2 did not affect the NO/urea ratio in macrophages from female rats, and diminished it in macrophages from both young and middle-aged male rats. Independently of the sex and age, E2 stimulated the release of inflammatory cytokines predominantly via macrophage ER alpha, and inhibited the IL-1 beta release in young females via ER beta. In contrast, E2 increased macrophage H2O2 and urea production by activating ER beta, but diminished their release via ER alpha. Our study may contribute to better understanding of the complex role(s) that E2 may play in innate immunity during aging, and that are dependent of sex.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats",
pages = "94-86",
volume = "113",
doi = "10.1016/j.exger.2018.09.024"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Petrović, R., Prijić, I.,& Vujić, V.. (2018). The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 113, 86-94.
https://doi.org/10.1016/j.exger.2018.09.024

Stanojević S, Ćuruvija I, Blagojević V, Petrović R, Prijić I, Vujić V. The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats. in Experimental Gerontology. 2018;113:86-94.
doi:10.1016/j.exger.2018.09.024 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Prijić, Ivana, Vujić, Vesna, "The involvement of estrogen receptors alpha and beta in the in vitro effects of 17 beta-estradiol on secretory profile of peritoneal macrophages from naturally menopausal female and middle-aged male rats" in Experimental Gerontology, 113 (2018):86-94,
https://doi.org/10.1016/j.exger.2018.09.024 . .

TY  - JOUR
AU  - Ćuruvija, Ivana
AU  - Stanojević, Stanislava
AU  - Arsenović-Ranin, Nevena
AU  - Blagojević, Veljko
AU  - Dimitrijević, Mirjana
AU  - Vidić-Danković, Biljana
AU  - Vujić, Vesna
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/496
AB  - The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression
EP  - 1101
IS  - 3
SP  - 1087
VL  - 40
DO  - 10.1007/s10753-017-0551-3
ER  - 

@article{
author = "Ćuruvija, Ivana and Stanojević, Stanislava and Arsenović-Ranin, Nevena and Blagojević, Veljko and Dimitrijević, Mirjana and Vidić-Danković, Biljana and Vujić, Vesna",
year = "2017",
abstract = "The aim of this study was to examine the influence of sex on age-related changes in phenotype and functional capacity of rat macrophages. The potential role of estradiol as a contributing factor to a sex difference in macrophage function with age was also examined. Thioglycollate-elicited peritoneal macrophages derived from the young (2 months old) and the naturally senescent intact middle-aged (16 months old) male and female rats were tested for cytokine secretion and antimicrobial activity (NO and H2O2 production and myeloperoxidase activity). Serum concentration of estradiol and the expression of estrogen receptor (ER)alpha and ER beta on freshly isolated peritoneal macrophages were also examined. Decreased secretion of IL-1 beta and IL-6 by macrophages from middle-aged compared to the young females was accompanied with the lesser density of macrophage ER alpha expression and the lower systemic level of estradiol, whereas the opposite was true for middle-aged male rats. Macrophages in the middle-aged females, even with the diminished circulating estradiol levels, produce increased amount of IL-6, and comparable amounts of IL-1 beta, TNF-alpha, and NO to that measured in macrophages from the middle-aged males. Age-related changes in macrophage phenotype and the antimicrobial activity were independent of macrophage ER alpha/ER beta expression and estradiol level in both male and female rats. Although our study suggests that the sex difference in the level of circulating estradiol may to some extent contribute to sex difference in macrophage function of middle-aged rats, it also points to more complex hormonal regulation of peritoneal macrophage activity in females.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression",
pages = "1101-1087",
number = "3",
volume = "40",
doi = "10.1007/s10753-017-0551-3"
}

Ćuruvija, I., Stanojević, S., Arsenović-Ranin, N., Blagojević, V., Dimitrijević, M., Vidić-Danković, B.,& Vujić, V.. (2017). Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation
Springer/Plenum Publishers, New York., 40(3), 1087-1101.
https://doi.org/10.1007/s10753-017-0551-3

Ćuruvija I, Stanojević S, Arsenović-Ranin N, Blagojević V, Dimitrijević M, Vidić-Danković B, Vujić V. Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression. in Inflammation. 2017;40(3):1087-1101.
doi:10.1007/s10753-017-0551-3 .

Ćuruvija, Ivana, Stanojević, Stanislava, Arsenović-Ranin, Nevena, Blagojević, Veljko, Dimitrijević, Mirjana, Vidić-Danković, Biljana, Vujić, Vesna, "Sex Differences in Macrophage Functions in Middle-Aged Rats: Relevance of Estradiol Level and Macrophage Estrogen Receptor Expression" in Inflammation, 40, no. 3 (2017):1087-1101,
https://doi.org/10.1007/s10753-017-0551-3 . .

TY  - CONF
AU  - Petrović, Raisa
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujnović, Ivana
AU  - Arsenović-Ranin, Nevena
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/669
AB  - Cilj rada je bio da se ispita uticaj starenja na aktivnost makrofaga aktivisanih M1/M2 polarizujućim faktorima. Peritonealne rezidentne makrofage (rMf) i tioglikolatom-indukovane makrofage (trFm) mladih (3 meseca) i starih (18-19) meseci pacova su kultivisane u prisustvu stimulatora klasične (M1) – lipopolisaharida (LPS) i faktora stimulacije kolonija granulocita i makrofaga (GM-CSF), i alternativne (M2) aktivacije makrofaga – interleukina-4 (IL-4), ili u odsustvu poznatih simulatora. Ispitivana je sposobnost fagocitozr zimozana i sekrecije inflamatornih medijatora. Starenjem se povećavala učestalost makrofaga monocitnog porekla (CCR*7CD68* ćelije) u okviru populacije rMf, dok je u okviru populacije tgMf nađena povećana učestalost makrofaga najzrelijeg fenotipa (CD163*CD68* ćelije). Nijedan od ispitivanih stimulatora nije uticao na fagocitnu sposobnost rMf i tgMf. Povećana sekrecija IL-1β, IL-6 I IL-10 I smanjena sekrecija TGF-β u odgovoru na stimulaciju LPS-om je nađena kod rMf i tgMF pacova obe starosti. GM-CSF je povećao sekreciju  IL-1β i IL-6 kod rMf starih pacova i tgMf mladih pacova. Paradoksalno, IL-4 je povećao sekreciju pro-inflamatornih citokina,  IL-1β i IL-6, kod rMf starih pacova. Starenjem se metabolizam arginina i u rMf i u tgMf usmeravao ka sintezi uree. Rezultati su pokazali da sa starenjem tgMf gube sposobnost polatizacije pod uticajem GM-CSF, i da rMf pod uticajem IL-4 polarizuju prema pro-inflamatornom M1 sekretornom fenotipu. Gubitak kontrole sekrecije inflamatornih medijatora iz makrofaga u odgovoru na M1/M2 aktivatore mogao bi da doprinese povećanom riziku od oboljevanja od infektivnih i inflamatornih bolesti u starosti.
C3  - VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata
T1  - Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro
UR  - https://hdl.handle.net/21.15107/rcub_intor_669
ER  - 

@conference{
author = "Petrović, Raisa and Dimitrijević, Mirjana and Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujnović, Ivana and Arsenović-Ranin, Nevena and Vujić, Vesna and Leposavić, Gordana",
year = "2016",
abstract = "Cilj rada je bio da se ispita uticaj starenja na aktivnost makrofaga aktivisanih M1/M2 polarizujućim faktorima. Peritonealne rezidentne makrofage (rMf) i tioglikolatom-indukovane makrofage (trFm) mladih (3 meseca) i starih (18-19) meseci pacova su kultivisane u prisustvu stimulatora klasične (M1) – lipopolisaharida (LPS) i faktora stimulacije kolonija granulocita i makrofaga (GM-CSF), i alternativne (M2) aktivacije makrofaga – interleukina-4 (IL-4), ili u odsustvu poznatih simulatora. Ispitivana je sposobnost fagocitozr zimozana i sekrecije inflamatornih medijatora. Starenjem se povećavala učestalost makrofaga monocitnog porekla (CCR*7CD68* ćelije) u okviru populacije rMf, dok je u okviru populacije tgMf nađena povećana učestalost makrofaga najzrelijeg fenotipa (CD163*CD68* ćelije). Nijedan od ispitivanih stimulatora nije uticao na fagocitnu sposobnost rMf i tgMf. Povećana sekrecija IL-1β, IL-6 I IL-10 I smanjena sekrecija TGF-β u odgovoru na stimulaciju LPS-om je nađena kod rMf i tgMF pacova obe starosti. GM-CSF je povećao sekreciju  IL-1β i IL-6 kod rMf starih pacova i tgMf mladih pacova. Paradoksalno, IL-4 je povećao sekreciju pro-inflamatornih citokina,  IL-1β i IL-6, kod rMf starih pacova. Starenjem se metabolizam arginina i u rMf i u tgMf usmeravao ka sintezi uree. Rezultati su pokazali da sa starenjem tgMf gube sposobnost polatizacije pod uticajem GM-CSF, i da rMf pod uticajem IL-4 polarizuju prema pro-inflamatornom M1 sekretornom fenotipu. Gubitak kontrole sekrecije inflamatornih medijatora iz makrofaga u odgovoru na M1/M2 aktivatore mogao bi da doprinese povećanom riziku od oboljevanja od infektivnih i inflamatornih bolesti u starosti.",
journal = "VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata",
title = "Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro",
url = "https://hdl.handle.net/21.15107/rcub_intor_669"
}

Petrović, R., Dimitrijević, M., Stanojević, S., Blagojević, V., Ćuruvija, I., Vujnović, I., Arsenović-Ranin, N., Vujić, V.,& Leposavić, G.. (2016). Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro. in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata.
https://hdl.handle.net/21.15107/rcub_intor_669

Petrović R, Dimitrijević M, Stanojević S, Blagojević V, Ćuruvija I, Vujnović I, Arsenović-Ranin N, Vujić V, Leposavić G. Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro. in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata. 2016;.
https://hdl.handle.net/21.15107/rcub_intor_669 .

Petrović, Raisa, Dimitrijević, Mirjana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujnović, Ivana, Arsenović-Ranin, Nevena, Vujić, Vesna, Leposavić, Gordana, "Starenje utiče na M1/M2 polarizaciju rezidentnih peritonealnih makrofaga pacova stimulisanih in vitro" in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata (2016),
https://hdl.handle.net/21.15107/rcub_intor_669 .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujnović, Ivana
AU  - Petrović, Raisa
AU  - Arsenović-Ranin, Nevena
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/466
AB  - Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age.
PB  - Springer, New York
T2  - Biogerontology
T1  - Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4
EP  - 371
IS  - 2
SP  - 359
VL  - 17
DO  - 10.1007/s10522-015-9620-x
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujnović, Ivana and Petrović, Raisa and Arsenović-Ranin, Nevena and Vujić, Vesna and Leposavić, Gordana",
year = "2016",
abstract = "Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age.",
publisher = "Springer, New York",
journal = "Biogerontology",
title = "Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4",
pages = "371-359",
number = "2",
volume = "17",
doi = "10.1007/s10522-015-9620-x"
}

Dimitrijević, M., Stanojević, S., Blagojević, V., Ćuruvija, I., Vujnović, I., Petrović, R., Arsenović-Ranin, N., Vujić, V.,& Leposavić, G.. (2016). Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4. in Biogerontology
Springer, New York., 17(2), 359-371.
https://doi.org/10.1007/s10522-015-9620-x

Dimitrijević M, Stanojević S, Blagojević V, Ćuruvija I, Vujnović I, Petrović R, Arsenović-Ranin N, Vujić V, Leposavić G. Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4. in Biogerontology. 2016;17(2):359-371.
doi:10.1007/s10522-015-9620-x .

Dimitrijević, Mirjana, Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujnović, Ivana, Petrović, Raisa, Arsenović-Ranin, Nevena, Vujić, Vesna, Leposavić, Gordana, "Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4" in Biogerontology, 17, no. 2 (2016):359-371,
https://doi.org/10.1007/s10522-015-9620-x . .

TY  - CONF
AU  - Ćuruvija, Ivana
AU  - Stanislava, Stanojević
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Blagojević, Veljko
AU  - Leposavić, Gordana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/670
AB  - smanjena imunološka funkcija kod žena u menopauzi je uglavnom posledica nedostatka hormona ovarijuma u cirkulaciji. Supstituciona hormonska terapija najčešće podrazumeva nadoknadu estrogena, ali ne i progesterona. U ovom radu kao eksperimantalni model izolovane deficijencije progesterona korišćeni su pacovi stari 20 meseci ovarijektomisani na kraju reproduktivnog preioda (starosti 10 meseci) kod kojih je koncentracija estradiola (usled ekstragonadne sinteze) u nivou estradiola kod lažno ovarijektomisanih pacova iste starosti. Ispitivane je intraćelijska ekspresija receptora za estrogene (ER) i progesteron (PR) i sekrecija pro- i anti-inflamatornih citokina i krajnjih produkata metabolizma arginina u slezinskim i peritonealnim makrofagama, u bazalnim uslovima i nakon in vitro stimulacije sa lipopolisaharidom (LPS). Pokazano je da i peritonealne i slezinske makrofage ispoljavaju ER alfa i ER beta, kao i da ovarijektomija ne utiče na ekspresiju ER-a. Većina peritonealnih i slezinskih makrofaga je ispoljavala PR, a ovarijektomija je dovela do povećanja ekspresije PR samo u slezinskim makrofagama. Ovrijektomija je smanjila i sekreciju citokina iz slezinskih (IL-1beta) i peritonealnih makrofaga (TNF-alfa, IL-1beta, IL-10) i povećala sekreciju IL-10 iz slezinskih i TGF-beta iz peritonealnih makrofaga u bazalnim uslovima. Nakon stimulacije LPS-om, slezinske makrofage  ovarijektomisanih pacova su sekretovale manje TNF-alfa i više IL-10, dok su peritonealne makrofage sekretovale manje IL-1beta i TGF-beta nego ćelije istog porekla iz lažno-ovarijektomisanih pacova. Ovarijektomija je smanjila sintezu uree i u slezinskim i u peritonealnim makrofagama stimulisanim LPS-om. Dugotrajna izolovana deficijencija progesterona u post-reproduktivnom periodu narušava ravnotežu u produkciji pro-/anti-inflamatornih citokina slezinskih i peritonealnih makrofaga.
C3  - VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata
T1  - Promene u citokinskom sekretornom profilu makrofaga starih ženki pacova usled nedostatka progesterona u postreproduktivnom periodu
UR  - https://hdl.handle.net/21.15107/rcub_intor_670
ER  - 

@conference{
author = "Ćuruvija, Ivana and Stanislava, Stanojević and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana and Vujić, Vesna and Blagojević, Veljko and Leposavić, Gordana",
year = "2016",
abstract = "smanjena imunološka funkcija kod žena u menopauzi je uglavnom posledica nedostatka hormona ovarijuma u cirkulaciji. Supstituciona hormonska terapija najčešće podrazumeva nadoknadu estrogena, ali ne i progesterona. U ovom radu kao eksperimantalni model izolovane deficijencije progesterona korišćeni su pacovi stari 20 meseci ovarijektomisani na kraju reproduktivnog preioda (starosti 10 meseci) kod kojih je koncentracija estradiola (usled ekstragonadne sinteze) u nivou estradiola kod lažno ovarijektomisanih pacova iste starosti. Ispitivane je intraćelijska ekspresija receptora za estrogene (ER) i progesteron (PR) i sekrecija pro- i anti-inflamatornih citokina i krajnjih produkata metabolizma arginina u slezinskim i peritonealnim makrofagama, u bazalnim uslovima i nakon in vitro stimulacije sa lipopolisaharidom (LPS). Pokazano je da i peritonealne i slezinske makrofage ispoljavaju ER alfa i ER beta, kao i da ovarijektomija ne utiče na ekspresiju ER-a. Većina peritonealnih i slezinskih makrofaga je ispoljavala PR, a ovarijektomija je dovela do povećanja ekspresije PR samo u slezinskim makrofagama. Ovrijektomija je smanjila i sekreciju citokina iz slezinskih (IL-1beta) i peritonealnih makrofaga (TNF-alfa, IL-1beta, IL-10) i povećala sekreciju IL-10 iz slezinskih i TGF-beta iz peritonealnih makrofaga u bazalnim uslovima. Nakon stimulacije LPS-om, slezinske makrofage  ovarijektomisanih pacova su sekretovale manje TNF-alfa i više IL-10, dok su peritonealne makrofage sekretovale manje IL-1beta i TGF-beta nego ćelije istog porekla iz lažno-ovarijektomisanih pacova. Ovarijektomija je smanjila sintezu uree i u slezinskim i u peritonealnim makrofagama stimulisanim LPS-om. Dugotrajna izolovana deficijencija progesterona u post-reproduktivnom periodu narušava ravnotežu u produkciji pro-/anti-inflamatornih citokina slezinskih i peritonealnih makrofaga.",
journal = "VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata",
title = "Promene u citokinskom sekretornom profilu makrofaga starih ženki pacova usled nedostatka progesterona u postreproduktivnom periodu",
url = "https://hdl.handle.net/21.15107/rcub_intor_670"
}

Ćuruvija, I., Stanislava, S., Kovačević-Jovanović, V., Dimitrijević, M., Vujić, V., Blagojević, V.,& Leposavić, G.. (2016). Promene u citokinskom sekretornom profilu makrofaga starih ženki pacova usled nedostatka progesterona u postreproduktivnom periodu. in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata.
https://hdl.handle.net/21.15107/rcub_intor_670

Ćuruvija I, Stanislava S, Kovačević-Jovanović V, Dimitrijević M, Vujić V, Blagojević V, Leposavić G. Promene u citokinskom sekretornom profilu makrofaga starih ženki pacova usled nedostatka progesterona u postreproduktivnom periodu. in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata. 2016;.
https://hdl.handle.net/21.15107/rcub_intor_670 .

Ćuruvija, Ivana, Stanislava, Stanojević, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, Vujić, Vesna, Blagojević, Veljko, Leposavić, Gordana, "Promene u citokinskom sekretornom profilu makrofaga starih ženki pacova usled nedostatka progesterona u postreproduktivnom periodu" in VII Naučni sastanak Društva imunologa Srbije, Program i knjiga apstrakata (2016),
https://hdl.handle.net/21.15107/rcub_intor_670 .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Petrović, Raisa
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/453
AB  - Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-alpha, and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-alpha production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging
EP  - 107
SP  - 95
VL  - 85
DO  - 10.1016/j.exger.2016.10.005
ER  - 

@article{
author = "Stanojević, Stanislava and Ćuruvija, Ivana and Blagojević, Veljko and Petrović, Raisa and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2016",
abstract = "Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in prevention of unbalanced inflammatory response in advanced age. We have analysed the phenotype of unelicited and thioglycollate-elicited peritoneal macrophages from young and middle-aged DA and AO rats and tested functions of these cells following stimulation with lipopolysaccharide (LPS) in vitro. Unelicited cells from middle-aged DA rats produced higher amounts of proinflammatory mediators interleukin-6 (IL-6) and nitric oxide (NO), but have a diminished response to LPS stimulation then cells from young rats, in spite of increased frequency of TLR4- and CD14-expressing mature macrophages. Injection of thioglycollate robustly increased overall cytokine production in young rats' macrophages, while diminishing their response to LPS stimulation. In middle-aged DA rats injection of thioglycollate diminished IL-6 production, but increased it in response to LPS stimulation. Quite the contrary to DA rats, the macrophages from middle-aged AO rats have released diminished levels of TNF-alpha, and NO, whereas urea production was strongly increased, when compared to the macrophages from young rats. Although the thioglycollate injection has increased the proportion of CD86(+)MHCII(+) mature macrophages in young rats, and percentages of activated TLR4(+) macrophages in both age groups of AO rats, it has not affected the cytokine production in young rats' macrophages, and the TNF-alpha production in middle-aged rats' macrophages. Moreover, the injection of thioglycollate has robustly increased the production of urea in macrophages derived from both age groups of AO rats. Although middle-aged rats of both strains were healthy during experiment, differences between the inflammatory responses of peritoneal macrophages of middle-aged rats of these strains might be one of the contributing factors defining their health in their advanced age. Development of strategies for the prevention of undesirable inflammatory changes in the elderly would benefit from the prospective study of the middle-aged. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging",
pages = "107-95",
volume = "85",
doi = "10.1016/j.exger.2016.10.005"
}

Stanojević, S., Ćuruvija, I., Blagojević, V., Petrović, R., Vujić, V.,& Dimitrijević, M.. (2016). Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 85, 95-107.
https://doi.org/10.1016/j.exger.2016.10.005

Stanojević S, Ćuruvija I, Blagojević V, Petrović R, Vujić V, Dimitrijević M. Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging. in Experimental Gerontology. 2016;85:95-107.
doi:10.1016/j.exger.2016.10.005 .

Stanojević, Stanislava, Ćuruvija, Ivana, Blagojević, Veljko, Petrović, Raisa, Vujić, Vesna, Dimitrijević, Mirjana, "Strain-dependent response to stimulation in middle-aged rat macrophages: A quest after a useful indicator of healthy aging" in Experimental Gerontology, 85 (2016):95-107,
https://doi.org/10.1016/j.exger.2016.10.005 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/423
AB  - Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat
EP  - 456
IS  - 5
SP  - 445
VL  - 74
DO  - 10.1111/aji.12424
ER  - 

@article{
author = "Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana and Vujić, Vesna and Ćuruvija, Ivana and Blagojević, Veljko and Leposavić, Gordana",
year = "2015",
abstract = "Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat",
pages = "456-445",
number = "5",
volume = "74",
doi = "10.1111/aji.12424"
}

Stanojević, S., Kovačević-Jovanović, V., Dimitrijević, M., Vujić, V., Ćuruvija, I., Blagojević, V.,& Leposavić, G.. (2015). Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology
Wiley, Hoboken., 74(5), 445-456.
https://doi.org/10.1111/aji.12424

Stanojević S, Kovačević-Jovanović V, Dimitrijević M, Vujić V, Ćuruvija I, Blagojević V, Leposavić G. Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology. 2015;74(5):445-456.
doi:10.1111/aji.12424 .

Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, Vujić, Vesna, Ćuruvija, Ivana, Blagojević, Veljko, Leposavić, Gordana, "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat" in American Journal of Reproductive Immunology, 74, no. 5 (2015):445-456,
https://doi.org/10.1111/aji.12424 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/424
AB  - This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.
PB  - Springer/Plenum Publishers, New York
T2  - Inflammation
T1  - Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains
EP  - 1449
IS  - 4
SP  - 1434
VL  - 38
DO  - 10.1007/s10753-015-0118-0
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2015",
abstract = "This study investigated a putative contribution of mast cells and C-sensory fibers to differences in the development of inflammatory edema following the injection of concanavalin A (Con A) into the hind paws of Dark Agouti (DA) and Albino Oxford (AO) rats. The treatment of adult rats with mast cell-depletor compound 48/80 and neonatal depletion of C-sensory fibers independently revealed that leukocyte composition of the inflamed paws and lymph nodes during local inflammatory response to Con A was generally regulated in a similar way in DA and AO rat strains. However, in DA and AO rats, the decrease and the increase of Con A-induced plasma extravasation were associated with mast cell depletion and activation, respectively, whereas neonatal capsaicin treatment activated dermal mast cells and potentiated inflammatory plasma extravasation only in adult rats of DA strain. Hence, strain differences in Emphasis Type="Strikethrough" the development of the inflammatory response to Con A are probably controlled by the differences in the interplay between mast cells and C-sensory fibers in DA and AO rats.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Inflammation",
title = "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains",
pages = "1449-1434",
number = "4",
volume = "38",
doi = "10.1007/s10753-015-0118-0"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V.,& Dimitrijević, M.. (2015). Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation
Springer/Plenum Publishers, New York., 38(4), 1434-1449.
https://doi.org/10.1007/s10753-015-0118-0

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Dimitrijević M. Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains. in Inflammation. 2015;38(4):1434-1449.
doi:10.1007/s10753-015-0118-0 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, "Role of Mast Cells and C-Sensory Fibers in Concanavalin A-Induced Paw Edema in Two Rat Strains" in Inflammation, 38, no. 4 (2015):1434-1449,
https://doi.org/10.1007/s10753-015-0118-0 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/402
AB  - Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.
PB  - Springer, New York
T2  - Biogerontology
T1  - Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains
EP  - 486
IS  - 5
SP  - 475
VL  - 15
DO  - 10.1007/s10522-014-9513-4
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Vujić, Vesna and Aleksić, Iva and Pilipović, Ivan and Leposavić, Gordana",
year = "2014",
abstract = "Altered functions of macrophages with aging contribute to impairment of both innate and adaptive immunity in the elderly. The present study aimed to examine strain specificity of age-related changes in the phenotypic and functional characteristics of macrophages from DA and AO rats, which differ in average life span. Resident peritoneal macrophages from young (10-12 weeks old) and aged (98-104 weeks old) rats were tested for: (a) the surface expression of TLR4 and CD14; (b) the basal and LPS-induced production of TNF-alpha and IL-10; and (c) the basal and LPS-induced activity of iNOS and arginase, by measuring the levels of NO and urea, respectively, in the culture supernatants. Aging elevated TLR4 macrophage surface density in rats of both strains. Conversely, the age-related decrease in the surface density of CD14 co-receptor was detected only on macrophages from aged DA rats. Accordingly, with aging in DA rats, contrary to AO rats, upon LPS-stimulation both TNF-alpha and IL-10 levels decreased in culture supernatants. However, in rats of both strains TNF-alpha stimulation index (LPS-induced over basal production) remained stable with aging, but it was significantly greater in AO rats. Furthermore, with aging, IL-10 stimulation index decreased and increased in DA and AO rats, respectively. Age-related shift in urea stimulation index complied with the changes of IL-10 stimulation index during aging. In conclusion, the study suggests that the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats.",
publisher = "Springer, New York",
journal = "Biogerontology",
title = "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains",
pages = "486-475",
number = "5",
volume = "15",
doi = "10.1007/s10522-014-9513-4"
}

Dimitrijević, M., Stanojević, S., Vujić, V., Aleksić, I., Pilipović, I.,& Leposavić, G.. (2014). Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology
Springer, New York., 15(5), 475-486.
https://doi.org/10.1007/s10522-014-9513-4

Dimitrijević M, Stanojević S, Vujić V, Aleksić I, Pilipović I, Leposavić G. Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains. in Biogerontology. 2014;15(5):475-486.
doi:10.1007/s10522-014-9513-4 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Vujić, Vesna, Aleksić, Iva, Pilipović, Ivan, Leposavić, Gordana, "Aging oppositely affects TNF-alpha and IL-10 production by macrophages from different rat strains" in Biogerontology, 15, no. 5 (2014):475-486,
https://doi.org/10.1007/s10522-014-9513-4 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Aleksić, Iva
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Pilipović, Ivan
AU  - von Hoersten, Stephan
AU  - Leposavić, Gordana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/397
AB  - In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6-(adult), 24-(old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1 beta and IL-6) and antiinflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-alpha and IL-6 mRNAs, but greater amount of IL-1 beta mRNA. On the other hand, cells fromlong-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1 beta mRNA relative to adult rats. Consequently, IL-10/IL-1 beta mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained = 95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-alpha and IL-6 concentrations were lower; IL-1 beta concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from longlived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.
PB  - Springer, Dordrecht
T2  - AGE
T1  - Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro
IS  - 4
VL  - 36
DO  - 10.1007/s11357-014-9696-2
ER  - 

@article{
author = "Dimitrijević, Mirjana and Aleksić, Iva and Vujić, Vesna and Stanojević, Stanislava and Pilipović, Ivan and von Hoersten, Stephan and Leposavić, Gordana",
year = "2014",
abstract = "In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6-(adult), 24-(old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1 beta and IL-6) and antiinflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-alpha and IL-6 mRNAs, but greater amount of IL-1 beta mRNA. On the other hand, cells fromlong-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1 beta mRNA relative to adult rats. Consequently, IL-10/IL-1 beta mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained = 95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-alpha and IL-6 concentrations were lower; IL-1 beta concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from longlived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.",
publisher = "Springer, Dordrecht",
journal = "AGE",
title = "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro",
number = "4",
volume = "36",
doi = "10.1007/s11357-014-9696-2"
}

Dimitrijević, M., Aleksić, I., Vujić, V., Stanojević, S., Pilipović, I., von Hoersten, S.,& Leposavić, G.. (2014). Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. in AGE
Springer, Dordrecht., 36(4).
https://doi.org/10.1007/s11357-014-9696-2

Dimitrijević M, Aleksić I, Vujić V, Stanojević S, Pilipović I, von Hoersten S, Leposavić G. Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. in AGE. 2014;36(4).
doi:10.1007/s11357-014-9696-2 .

Dimitrijević, Mirjana, Aleksić, Iva, Vujić, Vesna, Stanojević, Stanislava, Pilipović, Ivan, von Hoersten, Stephan, Leposavić, Gordana, "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1 beta expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro" in AGE, 36, no. 4 (2014),
https://doi.org/10.1007/s11357-014-9696-2 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Dimitrijević, Mirjana
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/389
AB  - New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.
PB  - Wiley-Blackwell, Hoboken
T2  - Experimental Physiology
T1  - Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production
EP  - 678
IS  - 3
SP  - 665
VL  - 98
DO  - 10.1113/expphysiol.2012.070524
ER  - 

@article{
author = "Stanojević, Stanislava and Dimitrijević, Mirjana and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Leposavić, Gordana",
year = "2013",
abstract = "New Findings center dot What is the central question of this study? Glucocorticoids modulate extraglandular catecholamine metabolism and adrenoceptor expression in many cell types. Catecholamines modulate the production of inflammatory mediators by macrophages. It was hypothesized that adrenal hormones affect tumour necrosis factor- production in rat macrophages by altering the autocrine/paracrine action of catecholamines. center dot What is the main finding and its importance? In rat macrophages, adrenalectomy increased tyrosine hydroxylase expression, decreased monoamine oxidase-A mRNA expression (due to the absence of adrenal catecholamines and glucocorticoids, respectively) and augmented 2-adrenoceptor expression (due to lack of adrenal catecholamines). However, notwithstanding these changes, propranolol treatment increased lipopolysaccharide-stimulated tumour necrosis factor- production in macrophages from adrenalectomized and non-operated rats to a similar extent. Catecholamines modulate the production of inflammatory mediators by macrophages in an autocrine/paracrine manner. They also tune 2-adrenoceptor expression. Glucocorticoids influence catecholamine metabolism and adrenoceptor expression in many cell types. We hypothesized that adrenal hormones affect the production of tumour necrosis factor- (TNF-) and NO by macrophages by altering the modulatory influence of catecholamines. To prove the hypothesis, peritoneal exudate macrophages from propranolol-treated non-operated and adrenalectomized rats and from corticosterone-supplemented adrenalectomized rats were examined for lipopolysaccharide-stimulated NO and TNF- production in vitro and for expression of 2-adrenoceptors and major catecholamine-metabolizing enzymes. Glucocorticoid deprivation increased NO production by macrophages, whereas 4 days of propranolol treatment was ineffective in this respect. However, propranolol treatment, via 2-adrenoceptor blockade, increased production of TNF- by macrophages in both non-operated and adrenalectomized rats (showing dramatically enhanced TNF- production due to a lack of circulating glucocorticoids) for the same value. The expression of 2-adrenoceptor was increased in peritoneal macrophages that were freshly isolated from non-operated, propranolol-treated and adrenalectomized rats (due to adrenal catecholamine deficiency). Propranolol did not affect macrophage 2-adrenoceptor expression in adrenalectomized rats. Given that propranolol increased the density of macrophage tyrosine hydroxylase expression only in non-operated rats and affected the mRNA expression of monoamine oxidase-A in neither non-operated nor adrenalectomized animals, a significant influence of propranolol on peritoneal exudate cell noradrenaline content was found only in non-operated rats. A lack of circulating adrenal hormones also affected noradrenaline metabolism and content in peritoneal exudate cells including macrophages. Collectively, despite differences in the abundance of macrophage catecholamine2-adrenoceptor system components and in the TNF- response to lipopolysaccharide between adrenalectomized and non-operated rats, propranolol increased TNF- production by the same amount in macrophages from these two groups of animals.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Experimental Physiology",
title = "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production",
pages = "678-665",
number = "3",
volume = "98",
doi = "10.1113/expphysiol.2012.070524"
}

Stanojević, S., Dimitrijević, M., Kuštrimović, N., Mitić, K., Vujić, V.,& Leposavić, G.. (2013). Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology
Wiley-Blackwell, Hoboken., 98(3), 665-678.
https://doi.org/10.1113/expphysiol.2012.070524

Stanojević S, Dimitrijević M, Kuštrimović N, Mitić K, Vujić V, Leposavić G. Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production. in Experimental Physiology. 2013;98(3):665-678.
doi:10.1113/expphysiol.2012.070524 .

Stanojević, Stanislava, Dimitrijević, Mirjana, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Leposavić, Gordana, "Adrenal hormone deprivation affects macrophage catecholamine metabolism and 2-adrenoceptor density, but not propranolol stimulation of tumour necrosis factor- production" in Experimental Physiology, 98, no. 3 (2013):665-678,
https://doi.org/10.1113/expphysiol.2012.070524 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Radojević, Katarina
AU  - Leposavić, Gordana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/383
AB  - The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production
EP  - 1254
IS  - 11
SP  - 1243
VL  - 48
DO  - 10.1016/j.exger.2013.07.001
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Radojević, Katarina and Leposavić, Gordana",
year = "2013",
abstract = "The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production",
pages = "1254-1243",
number = "11",
volume = "48",
doi = "10.1016/j.exger.2013.07.001"
}

Dimitrijević, M., Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I., Radojević, K.,& Leposavić, G.. (2013). The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 48(11), 1243-1254.
https://doi.org/10.1016/j.exger.2013.07.001

Dimitrijević M, Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Radojević K, Leposavić G. The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology. 2013;48(11):1243-1254.
doi:10.1016/j.exger.2013.07.001 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Radojević, Katarina, Leposavić, Gordana, "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production" in Experimental Gerontology, 48, no. 11 (2013):1243-1254,
https://doi.org/10.1016/j.exger.2013.07.001 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Aleksić, Iva
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/382
AB  - Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats
EP  - 572
IS  - 16
SP  - 564
VL  - 93
DO  - 10.1016/j.lfs.2013.08.021
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Dimitrijević, Mirjana",
year = "2013",
abstract = "Aims: Macrophages are heterogeneous population of inflammatory cells and, in response to the microenvironment, become differentially activated. The objective of the study was to explore macrophage effector functions during different inflammatory conditions in two rat strains. Main methods: We have investigated the effects of in vivo treatment with mast cell-degranulating compound 48/80 and/or thioglycollate on peritoneal macrophage phagocytosis and capacity to secrete hydrogen peroxide (H2O2), tumor necrosis factor-alpha (INF-alpha) and nitric oxide (NO) in Dark Agouti (DA) and Albino Oxford (AO) rat strains. Besides, fresh peritoneal cells were examined for the expression of ED1, ED2 and CD86 molecules. Key findings: In thioglycollate-elicited macrophages, increased proportion of ED1 + cells was accompanied with elevated phagocytosis of zymosan (DA strain), whereas increased expression level of CD86 molecule on ED2 + macrophages matched elevated secretory capacity for H2O2, TNF-alpha and NO (AO rats). Although mast cell degranulation induced by compound 48/80 increased the percentages of ED2 + macrophages in both rat strains, the proportion of ED2 + cells expressing CD86 molecule was decreased and increased in DA and AO rats, respectively. Furthermore, in DA strain compound 48/80 diminished macrophage secretion of NO, but stimulated all macrophage functions tested in AO strain. If applied concomitantly, the compound 48/80 additively increased macrophage activity induced by thioglycollate in AO rats. Significance: Macrophages from DA and AO rat strains show different susceptibility to mediators released from mast cells, suggesting that strain-dependant predisposition(s) toward particular activation pattern is decisive for the macrophage efficacy in response to inflammatory agents. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats",
pages = "572-564",
number = "16",
volume = "93",
doi = "10.1016/j.lfs.2013.08.021"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I.,& Dimitrijević, M.. (2013). Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 93(16), 564-572.
https://doi.org/10.1016/j.lfs.2013.08.021

Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Dimitrijević M. Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats. in Life Sciences. 2013;93(16):564-572.
doi:10.1016/j.lfs.2013.08.021 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Dimitrijević, Mirjana, "Peritoneal mast cell degranulation differently affected thioglycollate-induced macrophage phenotype and activity in Dark Agouti and Albino Oxford rats" in Life Sciences, 93, no. 16 (2013):564-572,
https://doi.org/10.1016/j.lfs.2013.08.021 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/357
AB  - Neuropeptide Y (NPY) suppressed clinical experimental autoimmune encephalomyelitis (EAE) and reduced numbers of CD28+, CD11b+ and CD80+ cells among spinal cord infiltrating cells at the peak of disease in Dark Agouti rat strain. Suppression of EAE was accompanied by the reduced expression of costimulatory CD80 and CD86 molecules on ED1+ macrophages and OX62+ dendritic cells in draining lymph nodes during the inductive phase of EAE. An inhibitor of dipeptidyl peptidase 4, an enzyme which terminates the action of NPY on 11 receptor subtype, did not sustain the suppressive effect of NPY on the EAE development, suggesting involvement of Y2 and Y5 receptors. (C) 2012 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Neuroimmunology
T1  - NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions
EP  - 31
IS  - 1-2
SP  - 23
VL  - 245
DO  - 10.1016/j.jneuroim.2012.01.013
ER  - 

@article{
author = "Dimitrijević, Mirjana and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Stanojević, Stanislava",
year = "2012",
abstract = "Neuropeptide Y (NPY) suppressed clinical experimental autoimmune encephalomyelitis (EAE) and reduced numbers of CD28+, CD11b+ and CD80+ cells among spinal cord infiltrating cells at the peak of disease in Dark Agouti rat strain. Suppression of EAE was accompanied by the reduced expression of costimulatory CD80 and CD86 molecules on ED1+ macrophages and OX62+ dendritic cells in draining lymph nodes during the inductive phase of EAE. An inhibitor of dipeptidyl peptidase 4, an enzyme which terminates the action of NPY on 11 receptor subtype, did not sustain the suppressive effect of NPY on the EAE development, suggesting involvement of Y2 and Y5 receptors. (C) 2012 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions",
pages = "31-23",
number = "1-2",
volume = "245",
doi = "10.1016/j.jneuroim.2012.01.013"
}

Dimitrijević, M., Mitić, K., Kuštrimović, N., Vujić, V.,& Stanojević, S.. (2012). NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions. in Journal of Neuroimmunology
Elsevier, Amsterdam., 245(1-2), 23-31.
https://doi.org/10.1016/j.jneuroim.2012.01.013

Dimitrijević M, Mitić K, Kuštrimović N, Vujić V, Stanojević S. NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions. in Journal of Neuroimmunology. 2012;245(1-2):23-31.
doi:10.1016/j.jneuroim.2012.01.013 .

Dimitrijević, Mirjana, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Stanojević, Stanislava, "NPY suppressed development of experimental autoimmune encephalomyelitis in Dark Agouti rats by disrupting costimulatory molecule interactions" in Journal of Neuroimmunology, 245, no. 1-2 (2012):23-31,
https://doi.org/10.1016/j.jneuroim.2012.01.013 . .

TY  - JOUR
AU  - Mitić, Katarina
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/327
AB  - Neuropeptide Y (NPY) has been reported to be a potent anti-inflammatory peptide with ability to directly modulate activity of granulocytes and macrophages. The present study aimed to correlate the effects of NPY in vivo on lipopolysaccharide-induced air-pouch exudates cells and in vitro on peripheral blood leukocytes functions. The role of different Y receptors was examined using NPY-related peptides and antagonists with diverse subtype specificity and selectivity for Y receptors. Y1, Y2 and Y5 receptors were detected on air-pouch exudates cells (flow cytometry) and peripheral blood granulocytes (immunocito-chemistry). NPY in vivo reduced inflammatory cells accumulation into the air pouch, and decreased their adherence and phagocytic capacity via Y2/Y5 and Y1/Y2 receptors, respectively. Quite the opposite, NPY in vitro potentiated adhesiveness and phagocytosis of peripheral blood granulocytes and monocytes by activating Y1 receptor. The differences between in vivo and in vitro effects of NPY on rat inflammatory cells functions are mostly due to dipeptidyl peptidase 4 activity. In addition, suppressive effect of NPY in vivo is highly dependent on the local microenvironment, peptide truncation and specific Y receptors interplay. (C) 2011 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors
EP  - 1633
IS  - 8
SP  - 1626
VL  - 32
DO  - 10.1016/j.peptides.2011.06.007
ER  - 

@article{
author = "Mitić, Katarina and Stanojević, Stanislava and Kuštrimović, Nataša and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2011",
abstract = "Neuropeptide Y (NPY) has been reported to be a potent anti-inflammatory peptide with ability to directly modulate activity of granulocytes and macrophages. The present study aimed to correlate the effects of NPY in vivo on lipopolysaccharide-induced air-pouch exudates cells and in vitro on peripheral blood leukocytes functions. The role of different Y receptors was examined using NPY-related peptides and antagonists with diverse subtype specificity and selectivity for Y receptors. Y1, Y2 and Y5 receptors were detected on air-pouch exudates cells (flow cytometry) and peripheral blood granulocytes (immunocito-chemistry). NPY in vivo reduced inflammatory cells accumulation into the air pouch, and decreased their adherence and phagocytic capacity via Y2/Y5 and Y1/Y2 receptors, respectively. Quite the opposite, NPY in vitro potentiated adhesiveness and phagocytosis of peripheral blood granulocytes and monocytes by activating Y1 receptor. The differences between in vivo and in vitro effects of NPY on rat inflammatory cells functions are mostly due to dipeptidyl peptidase 4 activity. In addition, suppressive effect of NPY in vivo is highly dependent on the local microenvironment, peptide truncation and specific Y receptors interplay. (C) 2011 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors",
pages = "1633-1626",
number = "8",
volume = "32",
doi = "10.1016/j.peptides.2011.06.007"
}

Mitić, K., Stanojević, S., Kuštrimović, N., Vujić, V.,& Dimitrijević, M.. (2011). Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors. in Peptides
Elsevier Science Inc, New York., 32(8), 1626-1633.
https://doi.org/10.1016/j.peptides.2011.06.007

Mitić K, Stanojević S, Kuštrimović N, Vujić V, Dimitrijević M. Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors. in Peptides. 2011;32(8):1626-1633.
doi:10.1016/j.peptides.2011.06.007 .

Mitić, Katarina, Stanojević, Stanislava, Kuštrimović, Nataša, Vujić, Vesna, Dimitrijević, Mirjana, "Neuropeptide Y modulates functions of inflammatory cells in the rat: Distinct role for Y1, Y2 and Y5 receptors" in Peptides, 32, no. 8 (2011):1626-1633,
https://doi.org/10.1016/j.peptides.2011.06.007 . .

TY  - JOUR
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/326
AB  - The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection.
AB  - Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors
T1  - Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora
EP  - 132
IS  - 2-3
SP  - 119
VL  - 61
DO  - 10.2298/AVB1103119K
ER  - 

@article{
author = "Kuštrimović, Nataša and Mitić, Katarina and Dimitrijević, Mirjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava",
year = "2011",
abstract = "The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection., Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors, Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora",
pages = "132-119",
number = "2-3",
volume = "61",
doi = "10.2298/AVB1103119K"
}

Kuštrimović, N., Mitić, K., Dimitrijević, M., Vujić, V., Kovačević-Jovanović, V., Miletić, T.,& Stanojević, S.. (2011). Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 119-132.
https://doi.org/10.2298/AVB1103119K

Kuštrimović N, Mitić K, Dimitrijević M, Vujić V, Kovačević-Jovanović V, Miletić T, Stanojević S. Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd. 2011;61(2-3):119-132.
doi:10.2298/AVB1103119K .

Kuštrimović, Nataša, Mitić, Katarina, Dimitrijević, Mirjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors" in Acta veterinaria - Beograd, 61, no. 2-3 (2011):119-132,
https://doi.org/10.2298/AVB1103119K . .

TY  - CONF
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/325
PB  - Birkhauser Verlag Ag, Basel
C3  - Inflammation Research
T1  - Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells
EP  - 87
SP  - 87
VL  - 60
UR  - https://hdl.handle.net/21.15107/rcub_intor_325
ER  - 

@conference{
author = "Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2011",
publisher = "Birkhauser Verlag Ag, Basel",
journal = "Inflammation Research",
title = "Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells",
pages = "87-87",
volume = "60",
url = "https://hdl.handle.net/21.15107/rcub_intor_325"
}

Kuštrimović, N., Mitić, K., Vujić, V., Dimitrijević, M.,& Stanojević, S.. (2011). Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells. in Inflammation Research
Birkhauser Verlag Ag, Basel., 60, 87-87.
https://hdl.handle.net/21.15107/rcub_intor_325

Kuštrimović N, Mitić K, Vujić V, Dimitrijević M, Stanojević S. Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells. in Inflammation Research. 2011;60:87-87.
https://hdl.handle.net/21.15107/rcub_intor_325 .

Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Dimitrijević, Mirjana, Stanojević, Stanislava, "Concanavalin a-induced paw edema: the contribution of c-sensory neurons and mast cells" in Inflammation Research, 60 (2011):87-87,
https://hdl.handle.net/21.15107/rcub_intor_325 .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/298
AB  - It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Regulatory Peptides
T1  - Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity
EP  - 109
IS  - 1-3
SP  - 100
VL  - 159
DO  - 10.1016/j.regpep.2009.11.002
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2010",
abstract = "It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity",
pages = "109-100",
number = "1-3",
volume = "159",
doi = "10.1016/j.regpep.2009.11.002"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2010). Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 159(1-3), 100-109.
https://doi.org/10.1016/j.regpep.2009.11.002

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides. 2010;159(1-3):100-109.
doi:10.1016/j.regpep.2009.11.002 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity" in Regulatory Peptides, 159, no. 1-3 (2010):100-109,
https://doi.org/10.1016/j.regpep.2009.11.002 . .