TY  - DATA
AU  - Frohns, Antonia
AU  - Stojanović, Marijana
AU  - Barisani-Asenbauer, Talin
AU  - Kuratli, Jasmin
AU  - Borel, Nicole
AU  - Inić-Kanada, Aleksandra
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/639
AB  - Figure S1. Structure of guinea pig retina and cornea. Nuclear staining with DAPI of guinea pig retina and cornea. In the retina, three nuclear layers (ONL-outer nuclear layer, INL-inner nuclear layer, and GCL-ganglion cell layer) are present. In the ONL, the photoreceptor cells are located, with their outer segments (OS) projecting into the adjacent retinal pigment epithelium (RPE). The INL exhibit nuclei of horizontal-, bipolar-, Müller glia, and amacrine cells. The cornea consists of an epithelium (EP), the stroma (S) containing keratinocytes, and the endothelium (EN); Figure S2. Western blots of HSPs in the guinea pig retina and cornea 19 days after wIRA/VIS treatment. Exemplary western blots of HSP40, HSP60, HSP70, and HSP90 in the retina and cornea. Comparison of non-irradiated (no wIRA/VIS) tissues with ones subjected to single (wIRA/VIS 1×) or sequential (wIRA/VIS 2×) irradiation. The constitutively expressed protein vinculin served as a loading control; Figure S3. Localization of Vinculin in the guinea pig retina and cornea 19 days after wIRA/VIS treatment. Immunolabeling of Vinculin in retinal layers (OS-outer segments, ONL-outer nuclear layer, INL-inner nuclear layer, and GCL-ganglion cell layer) and corneal layers (EP-epithelium, S-Stroma, and EN-endothelium). Comparison of non-irradiated (no wIRA/VIS) tissues with ones subjected to single (wIRA/VIS 1×) or sequential (wIRA/VIS 2×) irradiation. Inserts in cornea images show magnified sections of EP and EN. Scale bars = 10 μm retina, 20 μm cornea; Figure S4. Western blots of stress-responsive proteins in the guinea pig retina and cornea 19 days after wIRA/VIS treatment. Exemplary western blots of Akt, pAkt, pPTEN, pcRaf, Erk1/2, and pErk1/2 in the retina and cornea. Comparison of non-irradiated (no wIRA/VIS) tissues with ones subjected to single (wIRA/VIS 1×) or sequential (wIRA/VIS 2×) irradiation. The constitutively expressed protein vinculin served as a loading control.
PB  - Elsevier
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Supplementary material for: Frohns, A.; Stojanović, M.; Barisani-Asenbauer, T.; Kuratli, J.; Borel, N.; Inić-Kanada, A. Effects of Water-Filtered Infrared A and Visible Light (WIRA/VIS) Radiation on Heat- and Stress-Responsive Proteins in the Retina and Cornea of Guinea Pigs. Journal of Photochemistry and Photobiology B: Biology 2021, 224, 112306. https://doi.org/10.1016/j.jphotobiol.2021.112306.
SP  - 112306
VL  - 224
UR  - https://hdl.handle.net/21.15107/rcub_intor_639
ER  - 

@misc{
author = "Frohns, Antonia and Stojanović, Marijana and Barisani-Asenbauer, Talin and Kuratli, Jasmin and Borel, Nicole and Inić-Kanada, Aleksandra",
year = "2021",
abstract = "Figure S1. Structure of guinea pig retina and cornea. Nuclear staining with DAPI of guinea pig retina and cornea. In the retina, three nuclear layers (ONL-outer nuclear layer, INL-inner nuclear layer, and GCL-ganglion cell layer) are present. In the ONL, the photoreceptor cells are located, with their outer segments (OS) projecting into the adjacent retinal pigment epithelium (RPE). The INL exhibit nuclei of horizontal-, bipolar-, Müller glia, and amacrine cells. The cornea consists of an epithelium (EP), the stroma (S) containing keratinocytes, and the endothelium (EN); Figure S2. Western blots of HSPs in the guinea pig retina and cornea 19 days after wIRA/VIS treatment. Exemplary western blots of HSP40, HSP60, HSP70, and HSP90 in the retina and cornea. Comparison of non-irradiated (no wIRA/VIS) tissues with ones subjected to single (wIRA/VIS 1×) or sequential (wIRA/VIS 2×) irradiation. The constitutively expressed protein vinculin served as a loading control; Figure S3. Localization of Vinculin in the guinea pig retina and cornea 19 days after wIRA/VIS treatment. Immunolabeling of Vinculin in retinal layers (OS-outer segments, ONL-outer nuclear layer, INL-inner nuclear layer, and GCL-ganglion cell layer) and corneal layers (EP-epithelium, S-Stroma, and EN-endothelium). Comparison of non-irradiated (no wIRA/VIS) tissues with ones subjected to single (wIRA/VIS 1×) or sequential (wIRA/VIS 2×) irradiation. Inserts in cornea images show magnified sections of EP and EN. Scale bars = 10 μm retina, 20 μm cornea; Figure S4. Western blots of stress-responsive proteins in the guinea pig retina and cornea 19 days after wIRA/VIS treatment. Exemplary western blots of Akt, pAkt, pPTEN, pcRaf, Erk1/2, and pErk1/2 in the retina and cornea. Comparison of non-irradiated (no wIRA/VIS) tissues with ones subjected to single (wIRA/VIS 1×) or sequential (wIRA/VIS 2×) irradiation. The constitutively expressed protein vinculin served as a loading control.",
publisher = "Elsevier",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Supplementary material for: Frohns, A.; Stojanović, M.; Barisani-Asenbauer, T.; Kuratli, J.; Borel, N.; Inić-Kanada, A. Effects of Water-Filtered Infrared A and Visible Light (WIRA/VIS) Radiation on Heat- and Stress-Responsive Proteins in the Retina and Cornea of Guinea Pigs. Journal of Photochemistry and Photobiology B: Biology 2021, 224, 112306. https://doi.org/10.1016/j.jphotobiol.2021.112306.",
pages = "112306",
volume = "224",
url = "https://hdl.handle.net/21.15107/rcub_intor_639"
}

Frohns, A., Stojanović, M., Barisani-Asenbauer, T., Kuratli, J., Borel, N.,& Inić-Kanada, A.. (2021). Supplementary material for: Frohns, A.; Stojanović, M.; Barisani-Asenbauer, T.; Kuratli, J.; Borel, N.; Inić-Kanada, A. Effects of Water-Filtered Infrared A and Visible Light (WIRA/VIS) Radiation on Heat- and Stress-Responsive Proteins in the Retina and Cornea of Guinea Pigs. Journal of Photochemistry and Photobiology B: Biology 2021, 224, 112306. https://doi.org/10.1016/j.jphotobiol.2021.112306.. in Journal of Photochemistry and Photobiology B: Biology
Elsevier., 224, 112306.
https://hdl.handle.net/21.15107/rcub_intor_639

Frohns A, Stojanović M, Barisani-Asenbauer T, Kuratli J, Borel N, Inić-Kanada A. Supplementary material for: Frohns, A.; Stojanović, M.; Barisani-Asenbauer, T.; Kuratli, J.; Borel, N.; Inić-Kanada, A. Effects of Water-Filtered Infrared A and Visible Light (WIRA/VIS) Radiation on Heat- and Stress-Responsive Proteins in the Retina and Cornea of Guinea Pigs. Journal of Photochemistry and Photobiology B: Biology 2021, 224, 112306. https://doi.org/10.1016/j.jphotobiol.2021.112306.. in Journal of Photochemistry and Photobiology B: Biology. 2021;224:112306.
https://hdl.handle.net/21.15107/rcub_intor_639 .

Frohns, Antonia, Stojanović, Marijana, Barisani-Asenbauer, Talin, Kuratli, Jasmin, Borel, Nicole, Inić-Kanada, Aleksandra, "Supplementary material for: Frohns, A.; Stojanović, M.; Barisani-Asenbauer, T.; Kuratli, J.; Borel, N.; Inić-Kanada, A. Effects of Water-Filtered Infrared A and Visible Light (WIRA/VIS) Radiation on Heat- and Stress-Responsive Proteins in the Retina and Cornea of Guinea Pigs. Journal of Photochemistry and Photobiology B: Biology 2021, 224, 112306. https://doi.org/10.1016/j.jphotobiol.2021.112306." in Journal of Photochemistry and Photobiology B: Biology, 224 (2021):112306,
https://hdl.handle.net/21.15107/rcub_intor_639 .

TY  - JOUR
AU  - Frohns, Antonia
AU  - Stojanović, Marijana
AU  - Barisani-Asenbauer, Talin
AU  - Kuratli, Jasmin
AU  - Borel, Nicole
AU  - Inić-Kanada, Aleksandra
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/617
AB  - Water-filtered infrared A and visible light (wIRA/VIS), shown to reduce chlamydial infections in vitro and in vivo, might represent an innovative therapeutic approach against trachoma, a neglected tropical disease caused by ocular infection with the bacterium C. trachomatis. In this in vivo study, we assessed the impact of wIRA radiation in combination with VIS (wavelength range 595–1400 nm, intensity 2100 W/m2) on the retina and cornea in a guinea pig animal model of inclusion conjunctivitis. We investigated the effects 19 days after wIRA/VIS irradiation by comparing a single and double wIRA/VIS treatment with a sham control. By immunolabeling and western blot analyses of critical heat- and stress-responsive proteins, we could not detect wIRA/VIS-induced changes in their expression pattern. Also, immunolabeling of specific retinal marker proteins revealed no changes in their expression pattern caused by the treatment. Our preclinical study suggests wIRA/VIS as a promising and safe therapeutic tool to treat ocular chlamydial infections.
PB  - Elsevier
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs
SP  - 112306
VL  - 224
DO  - 10.1016/j.jphotobiol.2021.112306
ER  - 

@article{
author = "Frohns, Antonia and Stojanović, Marijana and Barisani-Asenbauer, Talin and Kuratli, Jasmin and Borel, Nicole and Inić-Kanada, Aleksandra",
year = "2021",
abstract = "Water-filtered infrared A and visible light (wIRA/VIS), shown to reduce chlamydial infections in vitro and in vivo, might represent an innovative therapeutic approach against trachoma, a neglected tropical disease caused by ocular infection with the bacterium C. trachomatis. In this in vivo study, we assessed the impact of wIRA radiation in combination with VIS (wavelength range 595–1400 nm, intensity 2100 W/m2) on the retina and cornea in a guinea pig animal model of inclusion conjunctivitis. We investigated the effects 19 days after wIRA/VIS irradiation by comparing a single and double wIRA/VIS treatment with a sham control. By immunolabeling and western blot analyses of critical heat- and stress-responsive proteins, we could not detect wIRA/VIS-induced changes in their expression pattern. Also, immunolabeling of specific retinal marker proteins revealed no changes in their expression pattern caused by the treatment. Our preclinical study suggests wIRA/VIS as a promising and safe therapeutic tool to treat ocular chlamydial infections.",
publisher = "Elsevier",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs",
pages = "112306",
volume = "224",
doi = "10.1016/j.jphotobiol.2021.112306"
}

Frohns, A., Stojanović, M., Barisani-Asenbauer, T., Kuratli, J., Borel, N.,& Inić-Kanada, A.. (2021). Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs. in Journal of Photochemistry and Photobiology B: Biology
Elsevier., 224, 112306.
https://doi.org/10.1016/j.jphotobiol.2021.112306

Frohns A, Stojanović M, Barisani-Asenbauer T, Kuratli J, Borel N, Inić-Kanada A. Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs. in Journal of Photochemistry and Photobiology B: Biology. 2021;224:112306.
doi:10.1016/j.jphotobiol.2021.112306 .

Frohns, Antonia, Stojanović, Marijana, Barisani-Asenbauer, Talin, Kuratli, Jasmin, Borel, Nicole, Inić-Kanada, Aleksandra, "Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs" in Journal of Photochemistry and Photobiology B: Biology, 224 (2021):112306,
https://doi.org/10.1016/j.jphotobiol.2021.112306 . .

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Miljković, Radmila
AU  - Stein, Elisabeth
AU  - Filipović, Ana
AU  - Frohns, Antonia
AU  - Zoeller, Nadja
AU  - Kuratli, Jasmin
AU  - Barisani-Asenbauer, Talin
AU  - Borel, Nicole
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/564
AB  - Trachoma is a devastating neglected tropical disease caused by Chlamydia trachomatis and the leading global cause of infectious blindness. Although antibiotic treatment against trachoma is efficient (SAFE strategy), additional affordable therapeutic strategies are of high interest. Water-filtered infrared A and visible light (wIRA/VIS) irradiation has proven to reduce chlamydial infectivity in vitro and ex vivo. The aim of this study was to evaluate whether wIRA/VIS can reduce chlamydial infection load and/or ocular pathology in vivo, in a guinea pig model of inclusion conjunctivitis. Guinea pigs were infected with 1 x 10(6) inclusion-forming units/eye of Chlamydia caviae via the ocular conjunctiva on day 0. In infected animals, wIRA/VIS irradiation (2100 W/m(2)) was applied on day 2 (single treatment) and on days 2 and 4 (double treatment) post-infection (pi). wIRA/VIS reduced the clinical pathology score on days 7 and 14 pi and the conjunctival chlamydial load on days 2, 4, 7, and 14 pi in comparison with C. caviae-infected, not irradiated, controls. Furthermore, numbers of chlamydial inclusions were decreased in wIRA/VIS treated C. caviae-infected guinea pigs on day 21 pi compared to C. caviae-infected, non-irradiated, controls. Double treatment with wIRA/VIS (days 2 and 4 pi) was more efficient than a single treatment on day 2 pi. wIRA/VIS treatment did neither induce macroscopic nor histologic changes in ocular tissues. Our results indicate that wIRA/VIS shows promising efficacy to reduce chlamydial infectivity in vivo without causing irradiation related pathologies in the follow-up period. wIRA/VIS irradiation is a promising approach to reduce trachoma transmission and pathology of ocular chlamydial infection.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis
VL  - 209
DO  - 10.1016/j.jphotobiol.2020.111953
ER  - 

@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Miljković, Radmila and Stein, Elisabeth and Filipović, Ana and Frohns, Antonia and Zoeller, Nadja and Kuratli, Jasmin and Barisani-Asenbauer, Talin and Borel, Nicole",
year = "2020",
abstract = "Trachoma is a devastating neglected tropical disease caused by Chlamydia trachomatis and the leading global cause of infectious blindness. Although antibiotic treatment against trachoma is efficient (SAFE strategy), additional affordable therapeutic strategies are of high interest. Water-filtered infrared A and visible light (wIRA/VIS) irradiation has proven to reduce chlamydial infectivity in vitro and ex vivo. The aim of this study was to evaluate whether wIRA/VIS can reduce chlamydial infection load and/or ocular pathology in vivo, in a guinea pig model of inclusion conjunctivitis. Guinea pigs were infected with 1 x 10(6) inclusion-forming units/eye of Chlamydia caviae via the ocular conjunctiva on day 0. In infected animals, wIRA/VIS irradiation (2100 W/m(2)) was applied on day 2 (single treatment) and on days 2 and 4 (double treatment) post-infection (pi). wIRA/VIS reduced the clinical pathology score on days 7 and 14 pi and the conjunctival chlamydial load on days 2, 4, 7, and 14 pi in comparison with C. caviae-infected, not irradiated, controls. Furthermore, numbers of chlamydial inclusions were decreased in wIRA/VIS treated C. caviae-infected guinea pigs on day 21 pi compared to C. caviae-infected, non-irradiated, controls. Double treatment with wIRA/VIS (days 2 and 4 pi) was more efficient than a single treatment on day 2 pi. wIRA/VIS treatment did neither induce macroscopic nor histologic changes in ocular tissues. Our results indicate that wIRA/VIS shows promising efficacy to reduce chlamydial infectivity in vivo without causing irradiation related pathologies in the follow-up period. wIRA/VIS irradiation is a promising approach to reduce trachoma transmission and pathology of ocular chlamydial infection.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis",
volume = "209",
doi = "10.1016/j.jphotobiol.2020.111953"
}

Inić-Kanada, A., Stojanović, M., Miljković, R., Stein, E., Filipović, A., Frohns, A., Zoeller, N., Kuratli, J., Barisani-Asenbauer, T.,& Borel, N.. (2020). Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis. in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 209.
https://doi.org/10.1016/j.jphotobiol.2020.111953

Inić-Kanada A, Stojanović M, Miljković R, Stein E, Filipović A, Frohns A, Zoeller N, Kuratli J, Barisani-Asenbauer T, Borel N. Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis. in Journal of Photochemistry and Photobiology B-Biology. 2020;209.
doi:10.1016/j.jphotobiol.2020.111953 .

Inić-Kanada, Aleksandra, Stojanović, Marijana, Miljković, Radmila, Stein, Elisabeth, Filipović, Ana, Frohns, Antonia, Zoeller, Nadja, Kuratli, Jasmin, Barisani-Asenbauer, Talin, Borel, Nicole, "Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis" in Journal of Photochemistry and Photobiology B-Biology, 209 (2020),
https://doi.org/10.1016/j.jphotobiol.2020.111953 . .