TY  - CONF
AU  - Vlijm, Rifka
AU  - Li, Xue
AU  - Panić, Marko
AU  - Rüthnick, Diana
AU  - Hata, Shoji
AU  - Herrmannsdörfer, Frank
AU  - Kuner, Thomas
AU  - Heilemann, Mike
AU  - Engelhardt, Johann
AU  - Hell, Stefan W.
AU  - Schiebel, Elmar
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/843
AB  - The centrosome linker proteins C-Nap1, rootletin and CEP68 connect the
two centrosomes of a cell during interphase into one microtubule organizing centre. This coupling is important for cell migration, cilia formation and timing of mitotic spindle formation. Very little is known about
the structure of the centrosome linker. Here, we used STimulated Emission Depletion (STED)microscopy to show that each C-Nap1 ring at the
proximal end of the two centrioles organizes a rootletin ring and in addition multiple rootletin fibres that radiate outwards from the ring into the
cytoplasm. Rootletin filaments have a repeat organization of 75 nm and
bind CEP68 via its C-terminal spectrin repeat containing region in 75
nm intervals. CEP68 is essential in forming rootletin filaments that branch
off centrioles and modulates the thickness of rootletin fibres. Thus, the
centrosome linker consists of a vast network of repeating rootletin units
with C-Nap1 as ring organizer and CEP68 as filament modulator. The
punctual contact model is consistent with the biological properties of
the centrosome linker.
PB  - Biophysical Society
PB  - Elsevier
C3  - Biophysical Journal
T1  - STED Nanoscopy of the Centrosome Linker Reveals a CEP68-Organized, Periodic Rootletin Network Anchored to a C-Nap1 Ring at Centrioles
IS  - 3
SP  - 535a
VL  - 114
DO  - 10.1016/j.bpj.2017.11.2926
ER  - 

@conference{
author = "Vlijm, Rifka and Li, Xue and Panić, Marko and Rüthnick, Diana and Hata, Shoji and Herrmannsdörfer, Frank and Kuner, Thomas and Heilemann, Mike and Engelhardt, Johann and Hell, Stefan W. and Schiebel, Elmar",
year = "2018",
abstract = "The centrosome linker proteins C-Nap1, rootletin and CEP68 connect the
two centrosomes of a cell during interphase into one microtubule organizing centre. This coupling is important for cell migration, cilia formation and timing of mitotic spindle formation. Very little is known about
the structure of the centrosome linker. Here, we used STimulated Emission Depletion (STED)microscopy to show that each C-Nap1 ring at the
proximal end of the two centrioles organizes a rootletin ring and in addition multiple rootletin fibres that radiate outwards from the ring into the
cytoplasm. Rootletin filaments have a repeat organization of 75 nm and
bind CEP68 via its C-terminal spectrin repeat containing region in 75
nm intervals. CEP68 is essential in forming rootletin filaments that branch
off centrioles and modulates the thickness of rootletin fibres. Thus, the
centrosome linker consists of a vast network of repeating rootletin units
with C-Nap1 as ring organizer and CEP68 as filament modulator. The
punctual contact model is consistent with the biological properties of
the centrosome linker.",
publisher = "Biophysical Society, Elsevier",
journal = "Biophysical Journal",
title = "STED Nanoscopy of the Centrosome Linker Reveals a CEP68-Organized, Periodic Rootletin Network Anchored to a C-Nap1 Ring at Centrioles",
number = "3",
pages = "535a",
volume = "114",
doi = "10.1016/j.bpj.2017.11.2926"
}

Vlijm, R., Li, X., Panić, M., Rüthnick, D., Hata, S., Herrmannsdörfer, F., Kuner, T., Heilemann, M., Engelhardt, J., Hell, S. W.,& Schiebel, E.. (2018). STED Nanoscopy of the Centrosome Linker Reveals a CEP68-Organized, Periodic Rootletin Network Anchored to a C-Nap1 Ring at Centrioles. in Biophysical Journal
Biophysical Society., 114(3), 535a.
https://doi.org/10.1016/j.bpj.2017.11.2926

Vlijm R, Li X, Panić M, Rüthnick D, Hata S, Herrmannsdörfer F, Kuner T, Heilemann M, Engelhardt J, Hell SW, Schiebel E. STED Nanoscopy of the Centrosome Linker Reveals a CEP68-Organized, Periodic Rootletin Network Anchored to a C-Nap1 Ring at Centrioles. in Biophysical Journal. 2018;114(3):535a.
doi:10.1016/j.bpj.2017.11.2926 .

Vlijm, Rifka, Li, Xue, Panić, Marko, Rüthnick, Diana, Hata, Shoji, Herrmannsdörfer, Frank, Kuner, Thomas, Heilemann, Mike, Engelhardt, Johann, Hell, Stefan W., Schiebel, Elmar, "STED Nanoscopy of the Centrosome Linker Reveals a CEP68-Organized, Periodic Rootletin Network Anchored to a C-Nap1 Ring at Centrioles" in Biophysical Journal, 114, no. 3 (2018):535a,
https://doi.org/10.1016/j.bpj.2017.11.2926 . .