TY  - CONF
AU  - Stojanović, M.
AU  - Živković, Irena
AU  - Inić-Kanada, A.
AU  - Petrušić, V.
AU  - Marinković, E.
AU  - Stojićević, I.
AU  - Dimitrijević, I.
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/711
AB  - Purpose/Objective: The hyperimmunization with tetanus toxoid
(TTd) induces protective TTd-specific as well as autoreactive b2-glycoprotein I (b2GPI)-specific immune responses in BALB/c mice. The
overall immune response characteristics, especially its pathogenic potential, depended on adjuvants applied prior and in combination with TTd. Beside structural homology between TTd and b2GPI, tolerance
toward b2GPI could be impaired by adjuvants acting as polyclonal
stimulators. In order to clarify the impact of adjuvants, phenotypic and
functional analyses of immune system cells within spleen were done
upon immunization completion.
Materials and methods: Non- or CFA-pretreated BALB/c mice were
immunized with TTd (3 · 100 lg/dose; 2-week intervals) mixed with
alum or 2.5M glycerol. Ex vivo analyses of CD3, CD4, CD8, CD19, CD
25, CD27 and mIgM expression on age-matched control and
immunized mice’s splenocytes were done by flow cytometry. Changes
in TLR2, TLR4 and TLR9 expression were assessed indirectly, by
measuring cytokine production, following in vitro stimulation of
splenocytes with appropriate agonist.
Results: TTd-immunization diminished CD27 expression on T cells
implying on their differentiation into potent effector cells. T cell
activation (increase in CD25 expression and the raise of percentage of
CD4+ CD8+ CD3+
) and B cell activation (rise in percentage of
CD19+ CD25+ cells and the increase of mIgM density) occurred in all
immunized mice, being more intensive in CFA-pretreated groups.
Irrespective to the applied immunization protocol, statistically significant rise in abundance of CD4- CD8- cells (often cited as cells having
suppressive potential) within T cell pool was registered too. Differences
in cytokines production (IL4, IL10, IFNc) registered upon in vitro
stimulation with peptidoglycan, LPS and CpG ODN implied on
context-dependant modulation of TLR2, TLR4 and TLR9 expression
on splenocytes.
Conclusions: TTd-hyperimmunization promoted concomitant rise in
abundance of activated cells and the cells that have suppressive
potential. This could be regarded as an attempt of the system to retain
control. Imbalance in percentages and activities between activated cells
and those having suppressive potential, highly influenced by the
context of TTd application, is most likely the cause for the observed
pathology appearance after TTd hyperimmunization.
PB  - Wiley
C3  - Immunology (Abstracts of the European Congress of Immunology, 5-8 September 2012, Glasgow, Scotland)
T1  - Phenotypic and functional characteristics of splenocytes in tetanus toxoid-hyperimmunized Balb/c mice is influenced by the context of tetanus toxoid application
EP  - 423
IS  - s1
SP  - 423
VL  - 137
UR  - https://hdl.handle.net/21.15107/rcub_intor_711
ER  - 

@conference{
author = "Stojanović, M. and Živković, Irena and Inić-Kanada, A. and Petrušić, V. and Marinković, E. and Stojićević, I. and Dimitrijević, I.",
year = "2012",
abstract = "Purpose/Objective: The hyperimmunization with tetanus toxoid
(TTd) induces protective TTd-specific as well as autoreactive b2-glycoprotein I (b2GPI)-specific immune responses in BALB/c mice. The
overall immune response characteristics, especially its pathogenic potential, depended on adjuvants applied prior and in combination with TTd. Beside structural homology between TTd and b2GPI, tolerance
toward b2GPI could be impaired by adjuvants acting as polyclonal
stimulators. In order to clarify the impact of adjuvants, phenotypic and
functional analyses of immune system cells within spleen were done
upon immunization completion.
Materials and methods: Non- or CFA-pretreated BALB/c mice were
immunized with TTd (3 · 100 lg/dose; 2-week intervals) mixed with
alum or 2.5M glycerol. Ex vivo analyses of CD3, CD4, CD8, CD19, CD
25, CD27 and mIgM expression on age-matched control and
immunized mice’s splenocytes were done by flow cytometry. Changes
in TLR2, TLR4 and TLR9 expression were assessed indirectly, by
measuring cytokine production, following in vitro stimulation of
splenocytes with appropriate agonist.
Results: TTd-immunization diminished CD27 expression on T cells
implying on their differentiation into potent effector cells. T cell
activation (increase in CD25 expression and the raise of percentage of
CD4+ CD8+ CD3+
) and B cell activation (rise in percentage of
CD19+ CD25+ cells and the increase of mIgM density) occurred in all
immunized mice, being more intensive in CFA-pretreated groups.
Irrespective to the applied immunization protocol, statistically significant rise in abundance of CD4- CD8- cells (often cited as cells having
suppressive potential) within T cell pool was registered too. Differences
in cytokines production (IL4, IL10, IFNc) registered upon in vitro
stimulation with peptidoglycan, LPS and CpG ODN implied on
context-dependant modulation of TLR2, TLR4 and TLR9 expression
on splenocytes.
Conclusions: TTd-hyperimmunization promoted concomitant rise in
abundance of activated cells and the cells that have suppressive
potential. This could be regarded as an attempt of the system to retain
control. Imbalance in percentages and activities between activated cells
and those having suppressive potential, highly influenced by the
context of TTd application, is most likely the cause for the observed
pathology appearance after TTd hyperimmunization.",
publisher = "Wiley",
journal = "Immunology (Abstracts of the European Congress of Immunology, 5-8 September 2012, Glasgow, Scotland)",
title = "Phenotypic and functional characteristics of splenocytes in tetanus toxoid-hyperimmunized Balb/c mice is influenced by the context of tetanus toxoid application",
pages = "423-423",
number = "s1",
volume = "137",
url = "https://hdl.handle.net/21.15107/rcub_intor_711"
}

Stojanović, M., Živković, I., Inić-Kanada, A., Petrušić, V., Marinković, E., Stojićević, I.,& Dimitrijević, I.. (2012). Phenotypic and functional characteristics of splenocytes in tetanus toxoid-hyperimmunized Balb/c mice is influenced by the context of tetanus toxoid application. in Immunology (Abstracts of the European Congress of Immunology, 5-8 September 2012, Glasgow, Scotland)
Wiley., 137(s1), 423-423.
https://hdl.handle.net/21.15107/rcub_intor_711

Stojanović M, Živković I, Inić-Kanada A, Petrušić V, Marinković E, Stojićević I, Dimitrijević I. Phenotypic and functional characteristics of splenocytes in tetanus toxoid-hyperimmunized Balb/c mice is influenced by the context of tetanus toxoid application. in Immunology (Abstracts of the European Congress of Immunology, 5-8 September 2012, Glasgow, Scotland). 2012;137(s1):423-423.
https://hdl.handle.net/21.15107/rcub_intor_711 .

Stojanović, M., Živković, Irena, Inić-Kanada, A., Petrušić, V., Marinković, E., Stojićević, I., Dimitrijević, I., "Phenotypic and functional characteristics of splenocytes in tetanus toxoid-hyperimmunized Balb/c mice is influenced by the context of tetanus toxoid application" in Immunology (Abstracts of the European Congress of Immunology, 5-8 September 2012, Glasgow, Scotland), 137, no. s1 (2012):423-423,
https://hdl.handle.net/21.15107/rcub_intor_711 .