TY  - CONF
AU  - Blagojević, Gordan
AU  - Arsenijević, J.
AU  - Vučković, Olga
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/503
PB  - Wiley, Hoboken
C3  - Allergy
T1  - Monitoring change in ige/igg4 ratio has predictive significance for estimating efficacy of allergen-specific immunotherapy (ait) and enhances rendering clinical decisions during treatment
EP  - 617
SP  - 617
VL  - 73
UR  - https://hdl.handle.net/21.15107/rcub_intor_503
ER  - 

@conference{
author = "Blagojević, Gordan and Arsenijević, J. and Vučković, Olga",
year = "2018",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Monitoring change in ige/igg4 ratio has predictive significance for estimating efficacy of allergen-specific immunotherapy (ait) and enhances rendering clinical decisions during treatment",
pages = "617-617",
volume = "73",
url = "https://hdl.handle.net/21.15107/rcub_intor_503"
}

Blagojević, G., Arsenijević, J.,& Vučković, O.. (2018). Monitoring change in ige/igg4 ratio has predictive significance for estimating efficacy of allergen-specific immunotherapy (ait) and enhances rendering clinical decisions during treatment. in Allergy
Wiley, Hoboken., 73, 617-617.
https://hdl.handle.net/21.15107/rcub_intor_503

Blagojević G, Arsenijević J, Vučković O. Monitoring change in ige/igg4 ratio has predictive significance for estimating efficacy of allergen-specific immunotherapy (ait) and enhances rendering clinical decisions during treatment. in Allergy. 2018;73:617-617.
https://hdl.handle.net/21.15107/rcub_intor_503 .

Blagojević, Gordan, Arsenijević, J., Vučković, Olga, "Monitoring change in ige/igg4 ratio has predictive significance for estimating efficacy of allergen-specific immunotherapy (ait) and enhances rendering clinical decisions during treatment" in Allergy, 73 (2018):617-617,
https://hdl.handle.net/21.15107/rcub_intor_503 .

TY  - CONF
AU  - Blagojević, Gordan
AU  - Vučković, Olga
AU  - Arsenijević, J.
AU  - Bonaci-Nikolić, B.
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/436
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Significance of extending the diagnostic algorithm for sIgE to rApi m1, rVes V5 and CCD (MUXF3) in patients with hymenoptera sting allergy: importance for selecting insect venom species for allergen-specific immunotherapy
EP  - 262
SP  - 262
VL  - 70
UR  - https://hdl.handle.net/21.15107/rcub_intor_436
ER  - 

@conference{
author = "Blagojević, Gordan and Vučković, Olga and Arsenijević, J. and Bonaci-Nikolić, B.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Significance of extending the diagnostic algorithm for sIgE to rApi m1, rVes V5 and CCD (MUXF3) in patients with hymenoptera sting allergy: importance for selecting insect venom species for allergen-specific immunotherapy",
pages = "262-262",
volume = "70",
url = "https://hdl.handle.net/21.15107/rcub_intor_436"
}

Blagojević, G., Vučković, O., Arsenijević, J.,& Bonaci-Nikolić, B.. (2015). Significance of extending the diagnostic algorithm for sIgE to rApi m1, rVes V5 and CCD (MUXF3) in patients with hymenoptera sting allergy: importance for selecting insect venom species for allergen-specific immunotherapy. in Allergy
Wiley-Blackwell, Hoboken., 70, 262-262.
https://hdl.handle.net/21.15107/rcub_intor_436

Blagojević G, Vučković O, Arsenijević J, Bonaci-Nikolić B. Significance of extending the diagnostic algorithm for sIgE to rApi m1, rVes V5 and CCD (MUXF3) in patients with hymenoptera sting allergy: importance for selecting insect venom species for allergen-specific immunotherapy. in Allergy. 2015;70:262-262.
https://hdl.handle.net/21.15107/rcub_intor_436 .

Blagojević, Gordan, Vučković, Olga, Arsenijević, J., Bonaci-Nikolić, B., "Significance of extending the diagnostic algorithm for sIgE to rApi m1, rVes V5 and CCD (MUXF3) in patients with hymenoptera sting allergy: importance for selecting insect venom species for allergen-specific immunotherapy" in Allergy, 70 (2015):262-262,
https://hdl.handle.net/21.15107/rcub_intor_436 .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Vučković, Olga
AU  - Smiljanić, Katarina
AU  - Gavrović-Jankulović, Marija
AU  - Krsmanović, Vera
AU  - Burazer, Lidija
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/407
AB  - According to the data obtained from in vivo and in vitro testing in Serbia, a significant number of patients have allergic symptoms caused by grass pollen. We examined the protein composition of grass pollens (Dactylis glomerata, Lolium perenne and Phleum pratense) and cross-reactivity in patients allergic to grass pollen from our region. The grass pollen allergen extract was characterized by SDS-PAGE, while cross-reactivity of single grass pollens was revealed by immunoblot analysis. A high degree of cross-reactivity was demonstrated for all three single pollens in the sera of allergic patients compared to the grass pollen extract mixture. Confirmation of the existence of cross-reactivity between different antigenic sources facilitates the use of monovalent vaccines, which are easier to standardize and at the same time prevent further sensitization of patients and reduces adverse reactions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The importance of cross-reactivity in grass pollen allergy
EP  - 1155
IS  - 3
SP  - 1149
VL  - 66
DO  - 10.2298/ABS1403149A
ER  - 

@article{
author = "Aleksić, Ivana and Vučković, Olga and Smiljanić, Katarina and Gavrović-Jankulović, Marija and Krsmanović, Vera and Burazer, Lidija",
year = "2014",
abstract = "According to the data obtained from in vivo and in vitro testing in Serbia, a significant number of patients have allergic symptoms caused by grass pollen. We examined the protein composition of grass pollens (Dactylis glomerata, Lolium perenne and Phleum pratense) and cross-reactivity in patients allergic to grass pollen from our region. The grass pollen allergen extract was characterized by SDS-PAGE, while cross-reactivity of single grass pollens was revealed by immunoblot analysis. A high degree of cross-reactivity was demonstrated for all three single pollens in the sera of allergic patients compared to the grass pollen extract mixture. Confirmation of the existence of cross-reactivity between different antigenic sources facilitates the use of monovalent vaccines, which are easier to standardize and at the same time prevent further sensitization of patients and reduces adverse reactions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The importance of cross-reactivity in grass pollen allergy",
pages = "1155-1149",
number = "3",
volume = "66",
doi = "10.2298/ABS1403149A"
}

Aleksić, I., Vučković, O., Smiljanić, K., Gavrović-Jankulović, M., Krsmanović, V.,& Burazer, L.. (2014). The importance of cross-reactivity in grass pollen allergy. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(3), 1149-1155.
https://doi.org/10.2298/ABS1403149A

Aleksić I, Vučković O, Smiljanić K, Gavrović-Jankulović M, Krsmanović V, Burazer L. The importance of cross-reactivity in grass pollen allergy. in Archives of Biological Sciences. 2014;66(3):1149-1155.
doi:10.2298/ABS1403149A .

Aleksić, Ivana, Vučković, Olga, Smiljanić, Katarina, Gavrović-Jankulović, Marija, Krsmanović, Vera, Burazer, Lidija, "The importance of cross-reactivity in grass pollen allergy" in Archives of Biological Sciences, 66, no. 3 (2014):1149-1155,
https://doi.org/10.2298/ABS1403149A . .

TY  - CONF
AU  - Burazer, Lidija
AU  - Aleksić, I.
AU  - Vučković, Olga
AU  - Gavrović, Marija
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/380
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Importance of sIgE measurement in evaluation of Dermatophagoides pteronyssinus major allergens in pediatric population
EP  - 689
SP  - 689
VL  - 68
UR  - https://hdl.handle.net/21.15107/rcub_intor_380
ER  - 

@conference{
author = "Burazer, Lidija and Aleksić, I. and Vučković, Olga and Gavrović, Marija",
year = "2013",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Importance of sIgE measurement in evaluation of Dermatophagoides pteronyssinus major allergens in pediatric population",
pages = "689-689",
volume = "68",
url = "https://hdl.handle.net/21.15107/rcub_intor_380"
}

Burazer, L., Aleksić, I., Vučković, O.,& Gavrović, M.. (2013). Importance of sIgE measurement in evaluation of Dermatophagoides pteronyssinus major allergens in pediatric population. in Allergy
Wiley-Blackwell, Hoboken., 68, 689-689.
https://hdl.handle.net/21.15107/rcub_intor_380

Burazer L, Aleksić I, Vučković O, Gavrović M. Importance of sIgE measurement in evaluation of Dermatophagoides pteronyssinus major allergens in pediatric population. in Allergy. 2013;68:689-689.
https://hdl.handle.net/21.15107/rcub_intor_380 .

Burazer, Lidija, Aleksić, I., Vučković, Olga, Gavrović, Marija, "Importance of sIgE measurement in evaluation of Dermatophagoides pteronyssinus major allergens in pediatric population" in Allergy, 68 (2013):689-689,
https://hdl.handle.net/21.15107/rcub_intor_380 .

TY  - JOUR
AU  - Ognjenović, Jana
AU  - Milčić-Matić, Natalija
AU  - Smiljanić, Katarina
AU  - Vučković, Olga
AU  - Burazer, Lidija
AU  - Popović, Nikola
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/375
AB  - Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dog's sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the 13 fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the a fragment of pollen allergen Amb a 1.0201. (C) 2013 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Immunology and Immunopathology
T1  - Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis
EP  - 47
IS  - 1-2
SP  - 38
VL  - 155
DO  - 10.1016/j.vetimm.2013.06.005
ER  - 

@article{
author = "Ognjenović, Jana and Milčić-Matić, Natalija and Smiljanić, Katarina and Vučković, Olga and Burazer, Lidija and Popović, Nikola and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2013",
abstract = "Canine atopic dermatitis (CAD) is an immune system disorder that affects 10-15% of the canine population. Short ragweed (Ambrosia artemisiifolia) pollen represents one of the major seasonal sources of allergenic pollen proteins in Europe, particularly in the Pannonian valley of the Balkan region. In Serbia, about 66% of atopic dogs showed a positive intradermal skin test with its pollen extract, which is second to house dust mites. Therefore, characterization of Ambrosia artemisiifolia pollen components, in terms of defining major and minor allergens that induce clinically manifested allergic reaction in dogs, is important for valid diagnosis and efficient therapy. This study has, for the first time, characterized and identified major Ambrosia artemisiifolia allergens in CAD, using an immunoproteomic approach. To assess the prevalence of specific IgE in electrophoretically separated ragweed pollen proteins, individual reactivity of sera from dogs with CAD was analyzed and compared to the reactivity of sera from healthy dogs in the non-reducing conditions, which were found optimal for specific canine IgE detection. A specific IgE band (38 kDa) was recognized as the most dominant allergen in CAD, occurring in 81% of positive dog's sera. 2-D immunoblotting followed by a mass spectrometry peptide fingerprint analyses with pooled canine and human atopic sera, revealed that 38 kDa major Ambrosia atremisiifolia allergens in CAD were all five isoallergens of the Amb a 1 group (antigen E), including the previously named Amb a 2 (antigen K). In contrast to canine sera, human atopic sera also recognized lower mass allergens such as the 13 fragment of Amb a 1 and profilins (Amb a 8 variants). The most prominent ragweed proteins in CAD, represent, as in humans, variants of all five isoallergens of the Amb a 1 group (pectate lyase): Amb a 1.0101 and its natural variant E1XUL2, Amb a 1.0202, 1.0304, 1.0402 and the natural variant of Amb a 1.0501, E1XUM0, as well as the a fragment of pollen allergen Amb a 1.0201. (C) 2013 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Immunology and Immunopathology",
title = "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis",
pages = "47-38",
number = "1-2",
volume = "155",
doi = "10.1016/j.vetimm.2013.06.005"
}

Ognjenović, J., Milčić-Matić, N., Smiljanić, K., Vučković, O., Burazer, L., Popović, N., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2013). Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. in Veterinary Immunology and Immunopathology
Elsevier Science Bv, Amsterdam., 155(1-2), 38-47.
https://doi.org/10.1016/j.vetimm.2013.06.005

Ognjenović J, Milčić-Matić N, Smiljanić K, Vučković O, Burazer L, Popović N, Stanić-Vučinić D, Ćirković-Veličković T. Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. in Veterinary Immunology and Immunopathology. 2013;155(1-2):38-47.
doi:10.1016/j.vetimm.2013.06.005 .

Ognjenović, Jana, Milčić-Matić, Natalija, Smiljanić, Katarina, Vučković, Olga, Burazer, Lidija, Popović, Nikola, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis" in Veterinary Immunology and Immunopathology, 155, no. 1-2 (2013):38-47,
https://doi.org/10.1016/j.vetimm.2013.06.005 . .

TY  - JOUR
AU  - Grozdanović, Milica
AU  - Popović, Milica
AU  - Polović, Natalija
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Atanasković-Marković, Marina
AU  - Lindner, Buko
AU  - Petersen, Arnd
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/358
AB  - Actinidin, an abundant cysteine protease from kiwifruit, is a specific biomarker of isolated allergy to kiwifruit. This study evaluates the IgE-binding properties of biologically active and thermally inactivated actinidin. Employing two different activity assays (caseinolytic assay and zymogram with gelatin) we showed that actinidin obtained from kiwifruit extract under native conditions represents a mixture of inactive and active enzyme. The structural integrity of actinidin was confirmed by SDS-PAGE. Edman degradation, mass fingerprint and Western blot with polyclonal antibodies. Although it was capable of inducing positive skin prick test reactions, we failed to detect IgE reactivity of active actinidin in Western blot with patient sera. Thermally inactivated actinidin exhibited IgE reactivity both in vivo and in vitro, indicating that heat processed kiwifruit products may induce clinical reactivity. These findings imply that apart from the allergenic epitopes on its surface, actinidin also contains hidden epitopes inside the protein which become accessible to IgE upon thermal treatment. (C) 2011 Elsevier Ltd. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Evaluation of IgE reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergy
EP  - 1018
IS  - 3-4
SP  - 1013
VL  - 50
DO  - 10.1016/j.fct.2011.12.030
ER  - 

@article{
author = "Grozdanović, Milica and Popović, Milica and Polović, Natalija and Burazer, Lidija and Vučković, Olga and Atanasković-Marković, Marina and Lindner, Buko and Petersen, Arnd and Gavrović-Jankulović, Marija",
year = "2012",
abstract = "Actinidin, an abundant cysteine protease from kiwifruit, is a specific biomarker of isolated allergy to kiwifruit. This study evaluates the IgE-binding properties of biologically active and thermally inactivated actinidin. Employing two different activity assays (caseinolytic assay and zymogram with gelatin) we showed that actinidin obtained from kiwifruit extract under native conditions represents a mixture of inactive and active enzyme. The structural integrity of actinidin was confirmed by SDS-PAGE. Edman degradation, mass fingerprint and Western blot with polyclonal antibodies. Although it was capable of inducing positive skin prick test reactions, we failed to detect IgE reactivity of active actinidin in Western blot with patient sera. Thermally inactivated actinidin exhibited IgE reactivity both in vivo and in vitro, indicating that heat processed kiwifruit products may induce clinical reactivity. These findings imply that apart from the allergenic epitopes on its surface, actinidin also contains hidden epitopes inside the protein which become accessible to IgE upon thermal treatment. (C) 2011 Elsevier Ltd. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Evaluation of IgE reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergy",
pages = "1018-1013",
number = "3-4",
volume = "50",
doi = "10.1016/j.fct.2011.12.030"
}

Grozdanović, M., Popović, M., Polović, N., Burazer, L., Vučković, O., Atanasković-Marković, M., Lindner, B., Petersen, A.,& Gavrović-Jankulović, M.. (2012). Evaluation of IgE reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergy. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 50(3-4), 1013-1018.
https://doi.org/10.1016/j.fct.2011.12.030

Grozdanović M, Popović M, Polović N, Burazer L, Vučković O, Atanasković-Marković M, Lindner B, Petersen A, Gavrović-Jankulović M. Evaluation of IgE reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergy. in Food and Chemical Toxicology. 2012;50(3-4):1013-1018.
doi:10.1016/j.fct.2011.12.030 .

Grozdanović, Milica, Popović, Milica, Polović, Natalija, Burazer, Lidija, Vučković, Olga, Atanasković-Marković, Marina, Lindner, Buko, Petersen, Arnd, Gavrović-Jankulović, Marija, "Evaluation of IgE reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergy" in Food and Chemical Toxicology, 50, no. 3-4 (2012):1013-1018,
https://doi.org/10.1016/j.fct.2011.12.030 . .

TY  - JOUR
AU  - Burazer, Lidija
AU  - Milovanović, K.
AU  - Milovanović, Mina
AU  - Vučković, Olga
AU  - Ćirković-Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/320
AB  - House dust mite (HDM) allergy has different clinical and immunological patterns in different geographic regions. The impact of raw material of commercial Dermatophadoides pteronyssinus (Acari: Pyroglyphidae) mite bodies on the quality of allergen extracts for allergy diagnosis in the Serbian population has not been previously evaluated. House dust mite bodies obtained from manufacturers in Europe, South America and Australia were used in the preparation of allergen extracts for in vivo diagnosis and serological analysis in a group of 14 HDM-allergic adults. In the group of mite-allergic patients, there was no statistically significant difference in skin test reactivity (Wilcoxon matched pairs test) among the three HDM body extract preparations. In a CAP inhibition assay, two extracts (A and C) achieved maximum inhibition of  gt  90%, whereas extract B demonstrated a different inhibition slope and lower inhibition potential (80%). However, a remarkable difference in immunoglobulin E reactivity using Western blot analysis with individual patients' sera was observed in one of the preparations (extract B). These findings emphasize the need for the careful selection of starting material for the preparation of HDM diagnostic reagents intended for use in patients from geographically distinct regions as these preparations can have implications on the selection criteria for patient-tailored immunotherapy of HDM allergy.
PB  - Wiley, Hoboken
T2  - Medical and Veterinary Entomology
T1  - Impact of Dermatophagoides pteronyssinus mite body raw material on house dust mite allergy diagnosis in a Serbian population
EP  - 83
IS  - 1
SP  - 77
VL  - 25
DO  - 10.1111/j.1365-2915.2010.00906.x
ER  - 

@article{
author = "Burazer, Lidija and Milovanović, K. and Milovanović, Mina and Vučković, Olga and Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija",
year = "2011",
abstract = "House dust mite (HDM) allergy has different clinical and immunological patterns in different geographic regions. The impact of raw material of commercial Dermatophadoides pteronyssinus (Acari: Pyroglyphidae) mite bodies on the quality of allergen extracts for allergy diagnosis in the Serbian population has not been previously evaluated. House dust mite bodies obtained from manufacturers in Europe, South America and Australia were used in the preparation of allergen extracts for in vivo diagnosis and serological analysis in a group of 14 HDM-allergic adults. In the group of mite-allergic patients, there was no statistically significant difference in skin test reactivity (Wilcoxon matched pairs test) among the three HDM body extract preparations. In a CAP inhibition assay, two extracts (A and C) achieved maximum inhibition of  gt  90%, whereas extract B demonstrated a different inhibition slope and lower inhibition potential (80%). However, a remarkable difference in immunoglobulin E reactivity using Western blot analysis with individual patients' sera was observed in one of the preparations (extract B). These findings emphasize the need for the careful selection of starting material for the preparation of HDM diagnostic reagents intended for use in patients from geographically distinct regions as these preparations can have implications on the selection criteria for patient-tailored immunotherapy of HDM allergy.",
publisher = "Wiley, Hoboken",
journal = "Medical and Veterinary Entomology",
title = "Impact of Dermatophagoides pteronyssinus mite body raw material on house dust mite allergy diagnosis in a Serbian population",
pages = "83-77",
number = "1",
volume = "25",
doi = "10.1111/j.1365-2915.2010.00906.x"
}

Burazer, L., Milovanović, K., Milovanović, M., Vučković, O., Ćirković-Veličković, T.,& Gavrović-Jankulović, M.. (2011). Impact of Dermatophagoides pteronyssinus mite body raw material on house dust mite allergy diagnosis in a Serbian population. in Medical and Veterinary Entomology
Wiley, Hoboken., 25(1), 77-83.
https://doi.org/10.1111/j.1365-2915.2010.00906.x

Burazer L, Milovanović K, Milovanović M, Vučković O, Ćirković-Veličković T, Gavrović-Jankulović M. Impact of Dermatophagoides pteronyssinus mite body raw material on house dust mite allergy diagnosis in a Serbian population. in Medical and Veterinary Entomology. 2011;25(1):77-83.
doi:10.1111/j.1365-2915.2010.00906.x .

Burazer, Lidija, Milovanović, K., Milovanović, Mina, Vučković, Olga, Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, "Impact of Dermatophagoides pteronyssinus mite body raw material on house dust mite allergy diagnosis in a Serbian population" in Medical and Veterinary Entomology, 25, no. 1 (2011):77-83,
https://doi.org/10.1111/j.1365-2915.2010.00906.x . .

TY  - JOUR
AU  - Stanić, Dragana
AU  - Monogioudi, Evanthia
AU  - Dilek, Ercili
AU  - Radosavljević, Jelena
AU  - Atanasković-Marković, Marina
AU  - Vučković, Olga
AU  - Raija, Lantto
AU  - Mattinen, Maija
AU  - Buchert, Johanna
AU  - Ćirković-Veličković, Tanja
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/294
AB  - Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim of this study was to examine the effects of enzymatic crosslinking on IgE binding, allergenicity and digestion stability of beta-casein (CN). beta-CN was crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid and laccase/caffeic acid. The IgE binding to beta-CN was compared in vitro by CAP inhibition assay, ELISA inhibition as well as ex vivo by basophil activation assay. Crosslinked CNs were digested by simulated gastric fluid for 15 and 60 min and obtained digests analyzed for their ability to inhibit IgE binding by CAP inhibition assay and SDS-PAGE. The ability of crosslinked CNs to activate basophils was significantly reduced in seven patients in the case of CN crosslinked by laccase and moderately reduced in the case of tyrosinase/caffeic acid crosslinked CN (in two cow's milk allergy patients tested with different allergen concentrations). The response to various crosslinked CNs differed individually among patients' sera tested by ELISA inhibition assay. The presence of caffeic acid hampered digestion by pepsin, and this effect was most pronounced for the tyrosinase/caffeic acid crosslinked CN. The laccase/caffeic acid and mushroom tyrosinase/caffeic acid had the highest potential in mitigating IgE binding and allergenicity of the beta-CN out of all investigated enzymes. The presence of a small phenolic compound also increased digestion stability of beta-CN.
PB  - Wiley, Hoboken
T2  - Molecular Nutrition and Food Research
T1  - Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein
EP  - 1284
IS  - 9
SP  - 1273
VL  - 54
DO  - 10.1002/mnfr.200900184
ER  - 

@article{
author = "Stanić, Dragana and Monogioudi, Evanthia and Dilek, Ercili and Radosavljević, Jelena and Atanasković-Marković, Marina and Vučković, Olga and Raija, Lantto and Mattinen, Maija and Buchert, Johanna and Ćirković-Veličković, Tanja",
year = "2010",
abstract = "Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim of this study was to examine the effects of enzymatic crosslinking on IgE binding, allergenicity and digestion stability of beta-casein (CN). beta-CN was crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid and laccase/caffeic acid. The IgE binding to beta-CN was compared in vitro by CAP inhibition assay, ELISA inhibition as well as ex vivo by basophil activation assay. Crosslinked CNs were digested by simulated gastric fluid for 15 and 60 min and obtained digests analyzed for their ability to inhibit IgE binding by CAP inhibition assay and SDS-PAGE. The ability of crosslinked CNs to activate basophils was significantly reduced in seven patients in the case of CN crosslinked by laccase and moderately reduced in the case of tyrosinase/caffeic acid crosslinked CN (in two cow's milk allergy patients tested with different allergen concentrations). The response to various crosslinked CNs differed individually among patients' sera tested by ELISA inhibition assay. The presence of caffeic acid hampered digestion by pepsin, and this effect was most pronounced for the tyrosinase/caffeic acid crosslinked CN. The laccase/caffeic acid and mushroom tyrosinase/caffeic acid had the highest potential in mitigating IgE binding and allergenicity of the beta-CN out of all investigated enzymes. The presence of a small phenolic compound also increased digestion stability of beta-CN.",
publisher = "Wiley, Hoboken",
journal = "Molecular Nutrition and Food Research",
title = "Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein",
pages = "1284-1273",
number = "9",
volume = "54",
doi = "10.1002/mnfr.200900184"
}

Stanić, D., Monogioudi, E., Dilek, E., Radosavljević, J., Atanasković-Marković, M., Vučković, O., Raija, L., Mattinen, M., Buchert, J.,& Ćirković-Veličković, T.. (2010). Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein. in Molecular Nutrition and Food Research
Wiley, Hoboken., 54(9), 1273-1284.
https://doi.org/10.1002/mnfr.200900184

Stanić D, Monogioudi E, Dilek E, Radosavljević J, Atanasković-Marković M, Vučković O, Raija L, Mattinen M, Buchert J, Ćirković-Veličković T. Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein. in Molecular Nutrition and Food Research. 2010;54(9):1273-1284.
doi:10.1002/mnfr.200900184 .

Stanić, Dragana, Monogioudi, Evanthia, Dilek, Ercili, Radosavljević, Jelena, Atanasković-Marković, Marina, Vučković, Olga, Raija, Lantto, Mattinen, Maija, Buchert, Johanna, Ćirković-Veličković, Tanja, "Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein" in Molecular Nutrition and Food Research, 54, no. 9 (2010):1273-1284,
https://doi.org/10.1002/mnfr.200900184 . .

TY  - JOUR
AU  - Popović, Milica
AU  - Milovanović, Mina
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Hoffmann-Sommergruber, Karin
AU  - Knulst, Andre C.
AU  - Lindner, Buko
AU  - Petersen, Arnd
AU  - Jankov, Ratko
AU  - Gavrović-Jankulović, Marija
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/305
AB  - Kiwifruit has become a frequent cause of fruit allergy in the recent years. The molecular basis of type I hypersensitivity to kiwifruit is attributed to 11 IUIS allergens, with Act d 1, Act d 2 and Act d 5 characterized in extenso. Evaluation of the allergenic properties of Act d 4, a cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa) was performed in this study. Identity of the purified glycoprotein was determined by Edman degradation and by mass fingerprint whereby more than 90% of the primary structure of the mature kiwifruit cystatin was confirmed. Using MALDI TOF analysis, molecular masses of 10902.5 and 11055.2 Da were detected for Act d 4, respectively. Positive skin prick reactivity with Act d 4 was induced in three kiwifruit allergic patients, as well as the upregulation of CD63 and CD203c molecules in the basophile activation assay. The IgE reactivity was detected in dot blot analysis while Western blot analysis was negative using sera from six kiwifruit patients, suggesting the presence of conformational IgE epitopes on the Act d 4 molecule. As activator of effector cells in type I hypersensitivity Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy.
PB  - Wiley, Hoboken
T2  - Molecular Nutrition and Food Research
T1  - Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy
EP  - 380
IS  - 3
SP  - 373
VL  - 54
DO  - 10.1002/mnfr.200900035
ER  - 

@article{
author = "Popović, Milica and Milovanović, Mina and Burazer, Lidija and Vučković, Olga and Hoffmann-Sommergruber, Karin and Knulst, Andre C. and Lindner, Buko and Petersen, Arnd and Jankov, Ratko and Gavrović-Jankulović, Marija",
year = "2010",
abstract = "Kiwifruit has become a frequent cause of fruit allergy in the recent years. The molecular basis of type I hypersensitivity to kiwifruit is attributed to 11 IUIS allergens, with Act d 1, Act d 2 and Act d 5 characterized in extenso. Evaluation of the allergenic properties of Act d 4, a cysteine proteinase inhibitor from green kiwifruit (Actinidia deliciosa) was performed in this study. Identity of the purified glycoprotein was determined by Edman degradation and by mass fingerprint whereby more than 90% of the primary structure of the mature kiwifruit cystatin was confirmed. Using MALDI TOF analysis, molecular masses of 10902.5 and 11055.2 Da were detected for Act d 4, respectively. Positive skin prick reactivity with Act d 4 was induced in three kiwifruit allergic patients, as well as the upregulation of CD63 and CD203c molecules in the basophile activation assay. The IgE reactivity was detected in dot blot analysis while Western blot analysis was negative using sera from six kiwifruit patients, suggesting the presence of conformational IgE epitopes on the Act d 4 molecule. As activator of effector cells in type I hypersensitivity Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy.",
publisher = "Wiley, Hoboken",
journal = "Molecular Nutrition and Food Research",
title = "Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy",
pages = "380-373",
number = "3",
volume = "54",
doi = "10.1002/mnfr.200900035"
}

Popović, M., Milovanović, M., Burazer, L., Vučković, O., Hoffmann-Sommergruber, K., Knulst, A. C., Lindner, B., Petersen, A., Jankov, R.,& Gavrović-Jankulović, M.. (2010). Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. in Molecular Nutrition and Food Research
Wiley, Hoboken., 54(3), 373-380.
https://doi.org/10.1002/mnfr.200900035

Popović M, Milovanović M, Burazer L, Vučković O, Hoffmann-Sommergruber K, Knulst AC, Lindner B, Petersen A, Jankov R, Gavrović-Jankulović M. Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. in Molecular Nutrition and Food Research. 2010;54(3):373-380.
doi:10.1002/mnfr.200900035 .

Popović, Milica, Milovanović, Mina, Burazer, Lidija, Vučković, Olga, Hoffmann-Sommergruber, Karin, Knulst, Andre C., Lindner, Buko, Petersen, Arnd, Jankov, Ratko, Gavrović-Jankulović, Marija, "Cysteine proteinase inhibitor Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy" in Molecular Nutrition and Food Research, 54, no. 3 (2010):373-380,
https://doi.org/10.1002/mnfr.200900035 . .

TY  - CONF
AU  - Popović, Milica
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Jankov, Ratko
AU  - Gavrović-Jankulović, Marija
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/316
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Evaluation of allergenic properties of cysteine protease inhibitor from common ragweed
EP  - 323
SP  - 323
VL  - 65
UR  - https://hdl.handle.net/21.15107/rcub_intor_316
ER  - 

@conference{
author = "Popović, Milica and Burazer, Lidija and Vučković, Olga and Jankov, Ratko and Gavrović-Jankulović, Marija",
year = "2010",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Evaluation of allergenic properties of cysteine protease inhibitor from common ragweed",
pages = "323-323",
volume = "65",
url = "https://hdl.handle.net/21.15107/rcub_intor_316"
}

Popović, M., Burazer, L., Vučković, O., Jankov, R.,& Gavrović-Jankulović, M.. (2010). Evaluation of allergenic properties of cysteine protease inhibitor from common ragweed. in Allergy
Wiley-Blackwell, Hoboken., 65, 323-323.
https://hdl.handle.net/21.15107/rcub_intor_316

Popović M, Burazer L, Vučković O, Jankov R, Gavrović-Jankulović M. Evaluation of allergenic properties of cysteine protease inhibitor from common ragweed. in Allergy. 2010;65:323-323.
https://hdl.handle.net/21.15107/rcub_intor_316 .

Popović, Milica, Burazer, Lidija, Vučković, Olga, Jankov, Ratko, Gavrović-Jankulović, Marija, "Evaluation of allergenic properties of cysteine protease inhibitor from common ragweed" in Allergy, 65 (2010):323-323,
https://hdl.handle.net/21.15107/rcub_intor_316 .

TY  - CONF
AU  - Burazer, Lidija
AU  - Milovanović, K.
AU  - Ćirković-Veličković, Tanja
AU  - Vučković, Olga
AU  - Gavrović-Jankulović, Marija
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/308
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Investigation of sublingual allergen vaccines stability by ELISA inhibition method
EP  - 256
SP  - 255
VL  - 65
UR  - https://hdl.handle.net/21.15107/rcub_intor_308
ER  - 

@conference{
author = "Burazer, Lidija and Milovanović, K. and Ćirković-Veličković, Tanja and Vučković, Olga and Gavrović-Jankulović, Marija",
year = "2010",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Investigation of sublingual allergen vaccines stability by ELISA inhibition method",
pages = "256-255",
volume = "65",
url = "https://hdl.handle.net/21.15107/rcub_intor_308"
}

Burazer, L., Milovanović, K., Ćirković-Veličković, T., Vučković, O.,& Gavrović-Jankulović, M.. (2010). Investigation of sublingual allergen vaccines stability by ELISA inhibition method. in Allergy
Wiley-Blackwell, Hoboken., 65, 255-256.
https://hdl.handle.net/21.15107/rcub_intor_308

Burazer L, Milovanović K, Ćirković-Veličković T, Vučković O, Gavrović-Jankulović M. Investigation of sublingual allergen vaccines stability by ELISA inhibition method. in Allergy. 2010;65:255-256.
https://hdl.handle.net/21.15107/rcub_intor_308 .

Burazer, Lidija, Milovanović, K., Ćirković-Veličković, Tanja, Vučković, Olga, Gavrović-Jankulović, Marija, "Investigation of sublingual allergen vaccines stability by ELISA inhibition method" in Allergy, 65 (2010):255-256,
https://hdl.handle.net/21.15107/rcub_intor_308 .

TY  - JOUR
AU  - Stanić, Dragana
AU  - Radosavljević, Jelena
AU  - Polović, Natalija
AU  - Jadranin, Milka
AU  - Popović, Milica
AU  - Vučković, Olga
AU  - Burazer, Lidija
AU  - Jankov, Ratko
AU  - Veličković, Tanja
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/292
AB  - The use of enzymes may improve the functional properties of various food ingredients. The aim of this study was to examine the effects of proteolytic contaminants in phenol oxidases on β-lactoglobulin (BLG). In the presence of Trametes versicolor laccase and Agaricus bisporus tyrosinase, both variants of BLG (A and B) underwent removal of a peptide from the N-terminus. The truncated forms were more susceptible to digestion by pepsin. The truncation of BLG resulted from contaminating proteases and not due to the action of phenol oxidases. The removal of N-terminal peptides proceeded quickly, while the rest of the globular protein remained resistant to proteolysis for up to 3 h. In the case of the application of enzymes in food bioprocessing, it may be important to carefully monitor the effects of contaminating proteases in enzyme preparations used.
T2  - International Dairy Journal
T1  - Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation
EP  - 752
IS  - 12
SP  - 746
VL  - 19
DO  - 10.1016/j.idairyj.2009.05.008
ER  - 

@article{
author = "Stanić, Dragana and Radosavljević, Jelena and Polović, Natalija and Jadranin, Milka and Popović, Milica and Vučković, Olga and Burazer, Lidija and Jankov, Ratko and Veličković, Tanja",
year = "2009",
abstract = "The use of enzymes may improve the functional properties of various food ingredients. The aim of this study was to examine the effects of proteolytic contaminants in phenol oxidases on β-lactoglobulin (BLG). In the presence of Trametes versicolor laccase and Agaricus bisporus tyrosinase, both variants of BLG (A and B) underwent removal of a peptide from the N-terminus. The truncated forms were more susceptible to digestion by pepsin. The truncation of BLG resulted from contaminating proteases and not due to the action of phenol oxidases. The removal of N-terminal peptides proceeded quickly, while the rest of the globular protein remained resistant to proteolysis for up to 3 h. In the case of the application of enzymes in food bioprocessing, it may be important to carefully monitor the effects of contaminating proteases in enzyme preparations used.",
journal = "International Dairy Journal",
title = "Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation",
pages = "752-746",
number = "12",
volume = "19",
doi = "10.1016/j.idairyj.2009.05.008"
}

Stanić, D., Radosavljević, J., Polović, N., Jadranin, M., Popović, M., Vučković, O., Burazer, L., Jankov, R.,& Veličković, T.. (2009). Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation. in International Dairy Journal, 19(12), 746-752.
https://doi.org/10.1016/j.idairyj.2009.05.008

Stanić D, Radosavljević J, Polović N, Jadranin M, Popović M, Vučković O, Burazer L, Jankov R, Veličković T. Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation. in International Dairy Journal. 2009;19(12):746-752.
doi:10.1016/j.idairyj.2009.05.008 .

Stanić, Dragana, Radosavljević, Jelena, Polović, Natalija, Jadranin, Milka, Popović, Milica, Vučković, Olga, Burazer, Lidija, Jankov, Ratko, Veličković, Tanja, "Removal of N-terminal peptides from β-lactoglobulin by proteolytic contaminants in a commercial phenol oxidase preparation" in International Dairy Journal, 19, no. 12 (2009):746-752,
https://doi.org/10.1016/j.idairyj.2009.05.008 . .

TY  - JOUR
AU  - Milovanović, Katarina
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Atanasković-Marković, Marina
AU  - Ćirković-Veličković, Tanja
AU  - Jankov, Ratko
AU  - Gavrović-Jankulović, Marija
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/285
AB  - House dust mites (HDM) represent a major source of allergens, contributing to the increasing incidence of type I hypersensitivity disease worldwide. Over 30 different IgE-binding proteins from the HDM extract were detected. Although group 1 and 2 have been identified as major allergens, due to the safety and efficacy of allergy diagnosis and immunotherapy, there is a need to carefully evaluate the clinical relevance of other allergens present in the HDM extract. In regard to this, a high molecular mass allergen of about 68 kD was purified from the HDM extract using a combination of gel permeation chromatography and reversed-phase chromatography. The IgG and IgE reactivity of the purified protein were preserved during the purification process, as confirmed by Western blot analysis with polyclonal rabbit antibodies and dot blot analysis with a pool of sera from subjects with house dust mite allergy, respectively. In addition, the IgE reactivity was confirmed using ELISA testing with nine patient sera. The biological potency of the 68 kD allergen was confirmed by skin prick testing in five allergic subjects, suggesting that the high molecular mass allergen is a good candidate for component-resolved diagnosis of house dust mite allergy and eventual therapeutic treatment.
AB  - Grinje iz kućne prašine predstavljaju jedan od glavnih izvora alergena koji su u značajnoj meri doprineli porastu prvog tipa preosetljivosti. Preko 30 IgE-vezujućih proteina iz kućne prašine je detektovano do danas. Alergeni grupe 1 i 2 označeni su kao glavni alergeni kućne prašine. Međutim, da bi se poboljšala sigurnost i efikasnost dijagnoze i terapije alergijskih oboljenja izazvanih grinjama iz kućne prašine, neophodno je odrediti klinički značaj svih alergena iz ovog alergenskog izvora. U ovom radu izolovan je alergen visoke molekulske mase od 68 kD iz ekstrakta kućne prašine kombinovanjem gel-permeacione hromatografije i reversno-fazne hromatografije. IgG i IgE reaktivnost prečišćenog proteina je proverena u 'Western blot'-u i 'dot blot'-u sa poliklonskim zečijim antitelima na ekstrakt kućne prašine i 'pool'-om seruma osoba alergičnih na kućnu prašinu, redom. 64 % pacijenata je pokazalo IgE reaktivnost na prečišćeni protein u ELISA testu. Biološka reaktivnost prečišćenog alergena je potvrđena u kožnim probama na pet pacijenata, ukazujući da je prečišćen alergen dobar kandidat za dijagnozu alergije na kućnu prašinu pojedinačnim komponentama i eventualni terapeutski tretman.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus
T1  - Izolovanje i karakterizacija 68 kD alergena iz ekstrakta kućnih grinja
EP  - 522
IS  - 5
SP  - 513
VL  - 74
DO  - 10.2298/JSC0905513M
ER  - 

@article{
author = "Milovanović, Katarina and Burazer, Lidija and Vučković, Olga and Atanasković-Marković, Marina and Ćirković-Veličković, Tanja and Jankov, Ratko and Gavrović-Jankulović, Marija",
year = "2009",
abstract = "House dust mites (HDM) represent a major source of allergens, contributing to the increasing incidence of type I hypersensitivity disease worldwide. Over 30 different IgE-binding proteins from the HDM extract were detected. Although group 1 and 2 have been identified as major allergens, due to the safety and efficacy of allergy diagnosis and immunotherapy, there is a need to carefully evaluate the clinical relevance of other allergens present in the HDM extract. In regard to this, a high molecular mass allergen of about 68 kD was purified from the HDM extract using a combination of gel permeation chromatography and reversed-phase chromatography. The IgG and IgE reactivity of the purified protein were preserved during the purification process, as confirmed by Western blot analysis with polyclonal rabbit antibodies and dot blot analysis with a pool of sera from subjects with house dust mite allergy, respectively. In addition, the IgE reactivity was confirmed using ELISA testing with nine patient sera. The biological potency of the 68 kD allergen was confirmed by skin prick testing in five allergic subjects, suggesting that the high molecular mass allergen is a good candidate for component-resolved diagnosis of house dust mite allergy and eventual therapeutic treatment., Grinje iz kućne prašine predstavljaju jedan od glavnih izvora alergena koji su u značajnoj meri doprineli porastu prvog tipa preosetljivosti. Preko 30 IgE-vezujućih proteina iz kućne prašine je detektovano do danas. Alergeni grupe 1 i 2 označeni su kao glavni alergeni kućne prašine. Međutim, da bi se poboljšala sigurnost i efikasnost dijagnoze i terapije alergijskih oboljenja izazvanih grinjama iz kućne prašine, neophodno je odrediti klinički značaj svih alergena iz ovog alergenskog izvora. U ovom radu izolovan je alergen visoke molekulske mase od 68 kD iz ekstrakta kućne prašine kombinovanjem gel-permeacione hromatografije i reversno-fazne hromatografije. IgG i IgE reaktivnost prečišćenog proteina je proverena u 'Western blot'-u i 'dot blot'-u sa poliklonskim zečijim antitelima na ekstrakt kućne prašine i 'pool'-om seruma osoba alergičnih na kućnu prašinu, redom. 64 % pacijenata je pokazalo IgE reaktivnost na prečišćeni protein u ELISA testu. Biološka reaktivnost prečišćenog alergena je potvrđena u kožnim probama na pet pacijenata, ukazujući da je prečišćen alergen dobar kandidat za dijagnozu alergije na kućnu prašinu pojedinačnim komponentama i eventualni terapeutski tretman.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus, Izolovanje i karakterizacija 68 kD alergena iz ekstrakta kućnih grinja",
pages = "522-513",
number = "5",
volume = "74",
doi = "10.2298/JSC0905513M"
}

Milovanović, K., Burazer, L., Vučković, O., Atanasković-Marković, M., Ćirković-Veličković, T., Jankov, R.,& Gavrović-Jankulović, M.. (2009). Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 74(5), 513-522.
https://doi.org/10.2298/JSC0905513M

Milovanović K, Burazer L, Vučković O, Atanasković-Marković M, Ćirković-Veličković T, Jankov R, Gavrović-Jankulović M. Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus. in Journal of the Serbian Chemical Society. 2009;74(5):513-522.
doi:10.2298/JSC0905513M .

Milovanović, Katarina, Burazer, Lidija, Vučković, Olga, Atanasković-Marković, Marina, Ćirković-Veličković, Tanja, Jankov, Ratko, Gavrović-Jankulović, Marija, "Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus" in Journal of the Serbian Chemical Society, 74, no. 5 (2009):513-522,
https://doi.org/10.2298/JSC0905513M . .

TY  - CONF
AU  - Popović, Milica
AU  - Milovanović, Mina
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Knulst, Andre C.
AU  - Hoffmann-Sommergruber, Karin
AU  - Lindner, Buko
AU  - Petersen, Arnd
AU  - Jankov, Ratko
AU  - Gavrović-Jankulović, Marija
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/276
PB  - Wiley-Blackwell Publishing, Inc, Malden
C3  - Allergy
T1  - Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy
EP  - 271
SP  - 270
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_intor_276
ER  - 

@conference{
author = "Popović, Milica and Milovanović, Mina and Burazer, Lidija and Vučković, Olga and Knulst, Andre C. and Hoffmann-Sommergruber, Karin and Lindner, Buko and Petersen, Arnd and Jankov, Ratko and Gavrović-Jankulović, Marija",
year = "2009",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Allergy",
title = "Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy",
pages = "271-270",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_intor_276"
}

Popović, M., Milovanović, M., Burazer, L., Vučković, O., Knulst, A. C., Hoffmann-Sommergruber, K., Lindner, B., Petersen, A., Jankov, R.,& Gavrović-Jankulović, M.. (2009). Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. in Allergy
Wiley-Blackwell Publishing, Inc, Malden., 64, 270-271.
https://hdl.handle.net/21.15107/rcub_intor_276

Popović M, Milovanović M, Burazer L, Vučković O, Knulst AC, Hoffmann-Sommergruber K, Lindner B, Petersen A, Jankov R, Gavrović-Jankulović M. Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy. in Allergy. 2009;64:270-271.
https://hdl.handle.net/21.15107/rcub_intor_276 .

Popović, Milica, Milovanović, Mina, Burazer, Lidija, Vučković, Olga, Knulst, Andre C., Hoffmann-Sommergruber, Karin, Lindner, Buko, Petersen, Arnd, Jankov, Ratko, Gavrović-Jankulović, Marija, "Act d 4 is a functional allergen contributing to the clinical symptoms of kiwifruit allergy" in Allergy, 64 (2009):270-271,
https://hdl.handle.net/21.15107/rcub_intor_276 .

TY  - CONF
AU  - Grozdanović, Milica
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Ćirković-Veličković, Tanja
AU  - Jankov, Ratko
AU  - Gavrović-Jankulović, Marija
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/274
PB  - Wiley-Blackwell Publishing, Inc, Malden
C3  - Allergy
T1  - Characterisation of a thaumatin-like homologue from birch (Betula verrucosa) pollen
EP  - 249
SP  - 249
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_intor_274
ER  - 

@conference{
author = "Grozdanović, Milica and Burazer, Lidija and Vučković, Olga and Ćirković-Veličković, Tanja and Jankov, Ratko and Gavrović-Jankulović, Marija",
year = "2009",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Allergy",
title = "Characterisation of a thaumatin-like homologue from birch (Betula verrucosa) pollen",
pages = "249-249",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_intor_274"
}

Grozdanović, M., Burazer, L., Vučković, O., Ćirković-Veličković, T., Jankov, R.,& Gavrović-Jankulović, M.. (2009). Characterisation of a thaumatin-like homologue from birch (Betula verrucosa) pollen. in Allergy
Wiley-Blackwell Publishing, Inc, Malden., 64, 249-249.
https://hdl.handle.net/21.15107/rcub_intor_274

Grozdanović M, Burazer L, Vučković O, Ćirković-Veličković T, Jankov R, Gavrović-Jankulović M. Characterisation of a thaumatin-like homologue from birch (Betula verrucosa) pollen. in Allergy. 2009;64:249-249.
https://hdl.handle.net/21.15107/rcub_intor_274 .

Grozdanović, Milica, Burazer, Lidija, Vučković, Olga, Ćirković-Veličković, Tanja, Jankov, Ratko, Gavrović-Jankulović, Marija, "Characterisation of a thaumatin-like homologue from birch (Betula verrucosa) pollen" in Allergy, 64 (2009):249-249,
https://hdl.handle.net/21.15107/rcub_intor_274 .

TY  - CONF
AU  - Milovanović, Mina
AU  - Atanasković-Marković, Marina
AU  - Vučković, Olga
AU  - Becker, Wolf-Meinhard
AU  - Petersen, Arnd
AU  - Gavrović-Jankulović, Marija
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/271
PB  - Wiley-Blackwell Publishing, Inc, Malden
C3  - Allergy
T1  - Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy
EP  - 80
SP  - 80
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_intor_271
ER  - 

@conference{
author = "Milovanović, Mina and Atanasković-Marković, Marina and Vučković, Olga and Becker, Wolf-Meinhard and Petersen, Arnd and Gavrović-Jankulović, Marija",
year = "2009",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Allergy",
title = "Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy",
pages = "80-80",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_intor_271"
}

Milovanović, M., Atanasković-Marković, M., Vučković, O., Becker, W., Petersen, A.,& Gavrović-Jankulović, M.. (2009). Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy. in Allergy
Wiley-Blackwell Publishing, Inc, Malden., 64, 80-80.
https://hdl.handle.net/21.15107/rcub_intor_271

Milovanović M, Atanasković-Marković M, Vučković O, Becker W, Petersen A, Gavrović-Jankulović M. Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy. in Allergy. 2009;64:80-80.
https://hdl.handle.net/21.15107/rcub_intor_271 .

Milovanović, Mina, Atanasković-Marković, Marina, Vučković, Olga, Becker, Wolf-Meinhard, Petersen, Arnd, Gavrović-Jankulović, Marija, "Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy" in Allergy, 64 (2009):80-80,
https://hdl.handle.net/21.15107/rcub_intor_271 .

TY  - JOUR
AU  - Atanasković-Marković, Marina
AU  - Gavrović-Jankulović, Marija
AU  - Veličković, Tanja
AU  - Vučković, Olga
AU  - Ivanovski, P.
AU  - Nestorovic, Branimir
AU  - Čuturilo, G.
AU  - Simić, D.
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/264
AB  - Natural rubber latex is the second most implicated agent in intraoperative anaphylactic reactions. This report describes a case of intraoperative anaphylaxis occurring in a non-atopic fourteen-year-old girl undergoing multiple surgical procedures, but without spina bifida, in which latex surgical gloves were the main culprit for the anaphylactic reactions. Clinical manifestations of an anaphylactic reaction were also experienced during the examination of the possible cause of intraoperative anaphylaxis by skin prick testing with a latex allergen extract. Skin tests with anesthetics were negative. Specific IgE to latex was positive at 92.9 kUA/L (class 5). The molecular basis for the reported intraoperative anaphylaxis was ascribed to three low-molecular mass latex allergens (10-15 kD) detected in the brand of latex surgical gloves used during the operation. Given the potential of a dramatic outcome, latex allergy testing as a regular preoperative measure may contribute to the reduction of anaphylactic reactions during surgical interventions.
T2  - Iranian Journal of Allergy, Asthma and Immunology
T1  - Intraoperative anaphylactic shock in a child with no history of type I hypersensitivity
EP  - 99
IS  - 2
SP  - 97
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_intor_264
ER  - 

@article{
author = "Atanasković-Marković, Marina and Gavrović-Jankulović, Marija and Veličković, Tanja and Vučković, Olga and Ivanovski, P. and Nestorovic, Branimir and Čuturilo, G. and Simić, D.",
year = "2008",
abstract = "Natural rubber latex is the second most implicated agent in intraoperative anaphylactic reactions. This report describes a case of intraoperative anaphylaxis occurring in a non-atopic fourteen-year-old girl undergoing multiple surgical procedures, but without spina bifida, in which latex surgical gloves were the main culprit for the anaphylactic reactions. Clinical manifestations of an anaphylactic reaction were also experienced during the examination of the possible cause of intraoperative anaphylaxis by skin prick testing with a latex allergen extract. Skin tests with anesthetics were negative. Specific IgE to latex was positive at 92.9 kUA/L (class 5). The molecular basis for the reported intraoperative anaphylaxis was ascribed to three low-molecular mass latex allergens (10-15 kD) detected in the brand of latex surgical gloves used during the operation. Given the potential of a dramatic outcome, latex allergy testing as a regular preoperative measure may contribute to the reduction of anaphylactic reactions during surgical interventions.",
journal = "Iranian Journal of Allergy, Asthma and Immunology",
title = "Intraoperative anaphylactic shock in a child with no history of type I hypersensitivity",
pages = "99-97",
number = "2",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_intor_264"
}

Atanasković-Marković, M., Gavrović-Jankulović, M., Veličković, T., Vučković, O., Ivanovski, P., Nestorovic, B., Čuturilo, G.,& Simić, D.. (2008). Intraoperative anaphylactic shock in a child with no history of type I hypersensitivity. in Iranian Journal of Allergy, Asthma and Immunology, 7(2), 97-99.
https://hdl.handle.net/21.15107/rcub_intor_264

Atanasković-Marković M, Gavrović-Jankulović M, Veličković T, Vučković O, Ivanovski P, Nestorovic B, Čuturilo G, Simić D. Intraoperative anaphylactic shock in a child with no history of type I hypersensitivity. in Iranian Journal of Allergy, Asthma and Immunology. 2008;7(2):97-99.
https://hdl.handle.net/21.15107/rcub_intor_264 .

Atanasković-Marković, Marina, Gavrović-Jankulović, Marija, Veličković, Tanja, Vučković, Olga, Ivanovski, P., Nestorovic, Branimir, Čuturilo, G., Simić, D., "Intraoperative anaphylactic shock in a child with no history of type I hypersensitivity" in Iranian Journal of Allergy, Asthma and Immunology, 7, no. 2 (2008):97-99,
https://hdl.handle.net/21.15107/rcub_intor_264 .

TY  - CONF
AU  - Vučković, Olga
AU  - Burazer, Lidija
AU  - Blagojević, Gordan
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/256
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - A determination of ImmunoCAP specific IgG(4) antibodies as a possible diagnostic marker for monitoring of immunotherapy to mite
EP  - 632
SP  - 631
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_intor_256
ER  - 

@conference{
author = "Vučković, Olga and Burazer, Lidija and Blagojević, Gordan",
year = "2008",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "A determination of ImmunoCAP specific IgG(4) antibodies as a possible diagnostic marker for monitoring of immunotherapy to mite",
pages = "632-631",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_intor_256"
}

Vučković, O., Burazer, L.,& Blagojević, G.. (2008). A determination of ImmunoCAP specific IgG(4) antibodies as a possible diagnostic marker for monitoring of immunotherapy to mite. in Allergy
Blackwell Publishing, Oxford., 63, 631-632.
https://hdl.handle.net/21.15107/rcub_intor_256

Vučković O, Burazer L, Blagojević G. A determination of ImmunoCAP specific IgG(4) antibodies as a possible diagnostic marker for monitoring of immunotherapy to mite. in Allergy. 2008;63:631-632.
https://hdl.handle.net/21.15107/rcub_intor_256 .

Vučković, Olga, Burazer, Lidija, Blagojević, Gordan, "A determination of ImmunoCAP specific IgG(4) antibodies as a possible diagnostic marker for monitoring of immunotherapy to mite" in Allergy, 63 (2008):631-632,
https://hdl.handle.net/21.15107/rcub_intor_256 .

TY  - CONF
AU  - Atanasković-Marković, Marina
AU  - Gavrović-Jankulović, Marija
AU  - Ćirković-Veličković, Tanja
AU  - Međo, B.
AU  - Kukolj, D.
AU  - Nestorovic, Branimir
AU  - Vučković, Olga
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/235
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - Intraoperative anaphylactic shock in a child with undiagnosed latex allergy
EP  - 80
SP  - 80
VL  - 62
UR  - https://hdl.handle.net/21.15107/rcub_intor_235
ER  - 

@conference{
author = "Atanasković-Marković, Marina and Gavrović-Jankulović, Marija and Ćirković-Veličković, Tanja and Međo, B. and Kukolj, D. and Nestorovic, Branimir and Vučković, Olga",
year = "2007",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "Intraoperative anaphylactic shock in a child with undiagnosed latex allergy",
pages = "80-80",
volume = "62",
url = "https://hdl.handle.net/21.15107/rcub_intor_235"
}

Atanasković-Marković, M., Gavrović-Jankulović, M., Ćirković-Veličković, T., Međo, B., Kukolj, D., Nestorovic, B.,& Vučković, O.. (2007). Intraoperative anaphylactic shock in a child with undiagnosed latex allergy. in Allergy
Blackwell Publishing, Oxford., 62, 80-80.
https://hdl.handle.net/21.15107/rcub_intor_235

Atanasković-Marković M, Gavrović-Jankulović M, Ćirković-Veličković T, Međo B, Kukolj D, Nestorovic B, Vučković O. Intraoperative anaphylactic shock in a child with undiagnosed latex allergy. in Allergy. 2007;62:80-80.
https://hdl.handle.net/21.15107/rcub_intor_235 .

Atanasković-Marković, Marina, Gavrović-Jankulović, Marija, Ćirković-Veličković, Tanja, Međo, B., Kukolj, D., Nestorovic, Branimir, Vučković, Olga, "Intraoperative anaphylactic shock in a child with undiagnosed latex allergy" in Allergy, 62 (2007):80-80,
https://hdl.handle.net/21.15107/rcub_intor_235 .

TY  - JOUR
AU  - Atanasković-Marković, Marina
AU  - Ćirković-Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Vučković, Olga
AU  - Nestorovic, Branimir
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/214
PB  - Blackwell Munksgaard
T2  - Pediatric Allergy and Immunology
T1  - Authors reply to beta-lactam allergy in children
EP  - 640
IS  - 8
SP  - 639
VL  - 17
DO  - 10.1111/j.1399-3038.2006.00462.x
ER  - 

@article{
author = "Atanasković-Marković, Marina and Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija and Vučković, Olga and Nestorovic, Branimir",
year = "2006",
publisher = "Blackwell Munksgaard",
journal = "Pediatric Allergy and Immunology",
title = "Authors reply to beta-lactam allergy in children",
pages = "640-639",
number = "8",
volume = "17",
doi = "10.1111/j.1399-3038.2006.00462.x"
}

Atanasković-Marković, M., Ćirković-Veličković, T., Gavrović-Jankulović, M., Vučković, O.,& Nestorovic, B.. (2006). Authors reply to beta-lactam allergy in children. in Pediatric Allergy and Immunology
Blackwell Munksgaard., 17(8), 639-640.
https://doi.org/10.1111/j.1399-3038.2006.00462.x

Atanasković-Marković M, Ćirković-Veličković T, Gavrović-Jankulović M, Vučković O, Nestorovic B. Authors reply to beta-lactam allergy in children. in Pediatric Allergy and Immunology. 2006;17(8):639-640.
doi:10.1111/j.1399-3038.2006.00462.x .

Atanasković-Marković, Marina, Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, Vučković, Olga, Nestorovic, Branimir, "Authors reply to beta-lactam allergy in children" in Pediatric Allergy and Immunology, 17, no. 8 (2006):639-640,
https://doi.org/10.1111/j.1399-3038.2006.00462.x . .

TY  - JOUR
AU  - Ćirković-Veličković, Tanja
AU  - Polović, Natalija
AU  - Gavrović-Jankulović, Marija
AU  - Burazer, Lidija
AU  - Đergović-Petrović, Danica
AU  - Vučković, Olga
AU  - Drobnjak, Olika
AU  - Šporčić, Zorica
AU  - Atanasković-Marković, Marina
AU  - Jankov, Ratko
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/220
AB  - Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones.
AB  - U ovom radu su prikazani rezultati ispitivanja stabilnosti tri tipa alergoida polena pelina u simuliranom želudačnom soku. Koristeći kalijum-cijanat anhidrid ćilibarne i anhidrid maleinske kiseline, napravljeni su alergoidi polena pelina (Artemisia vulgaris). Saliva i želudačni sok su simulirani na osnovu evropske farmakopeje. Biohemijske i imunohemijske osobine derivata posle izlaganja različitim uslovima, praćene su: određivanjem broja slobodnih amino grupa u reakciji sa TNBS, SDS PAG elektroforezom, imunoblotom i određivanjem pelin-specifičnog imunoglobulina E (IgE). Izlaganje salivi u trajanju od 2 minuta ne utiče na biohemijske i imunohemijske osobine derivata. U kiseloj sredini želudačnog soka ne dolazi do značajnog demaleilovawa i desukcinilovanja. Čak i posle četvoročasovnog izlaganja, taj procenat je u opsegu 10-30 %. Alergoidi pelina se trenutno digestuju pepsinom, sa izuzetkom visoko rezistentne proteinske trake molekulske mase 28-35 kD, koja odgovara važnom IgE-vezujućem proteinu polena pelina. Imunoblotom i CAP-inhibicijom je pokazano da, u okviru fizioloških uslova, ne dolazi do stvaranja novih IgE-vezujućih epitopa. Hemijska stabilnost modifikovanih derivata u simuliranim uslovima želudačnog soka omogućuje da se tokom imunoterapije mogu primenjivati veće doze alergoida nego nemodifikovanog ekstrakta polena pelina.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract
T1  - Digestibilnost alergoida polena pelina u simuliranim uslovima gastrointestinalnog trakta
EP  - 888
IS  - 8-9
SP  - 879
VL  - 71
DO  - 10.2298/JSC0609879C
ER  - 

@article{
author = "Ćirković-Veličković, Tanja and Polović, Natalija and Gavrović-Jankulović, Marija and Burazer, Lidija and Đergović-Petrović, Danica and Vučković, Olga and Drobnjak, Olika and Šporčić, Zorica and Atanasković-Marković, Marina and Jankov, Ratko",
year = "2006",
abstract = "Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones., U ovom radu su prikazani rezultati ispitivanja stabilnosti tri tipa alergoida polena pelina u simuliranom želudačnom soku. Koristeći kalijum-cijanat anhidrid ćilibarne i anhidrid maleinske kiseline, napravljeni su alergoidi polena pelina (Artemisia vulgaris). Saliva i želudačni sok su simulirani na osnovu evropske farmakopeje. Biohemijske i imunohemijske osobine derivata posle izlaganja različitim uslovima, praćene su: određivanjem broja slobodnih amino grupa u reakciji sa TNBS, SDS PAG elektroforezom, imunoblotom i određivanjem pelin-specifičnog imunoglobulina E (IgE). Izlaganje salivi u trajanju od 2 minuta ne utiče na biohemijske i imunohemijske osobine derivata. U kiseloj sredini želudačnog soka ne dolazi do značajnog demaleilovawa i desukcinilovanja. Čak i posle četvoročasovnog izlaganja, taj procenat je u opsegu 10-30 %. Alergoidi pelina se trenutno digestuju pepsinom, sa izuzetkom visoko rezistentne proteinske trake molekulske mase 28-35 kD, koja odgovara važnom IgE-vezujućem proteinu polena pelina. Imunoblotom i CAP-inhibicijom je pokazano da, u okviru fizioloških uslova, ne dolazi do stvaranja novih IgE-vezujućih epitopa. Hemijska stabilnost modifikovanih derivata u simuliranim uslovima želudačnog soka omogućuje da se tokom imunoterapije mogu primenjivati veće doze alergoida nego nemodifikovanog ekstrakta polena pelina.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract, Digestibilnost alergoida polena pelina u simuliranim uslovima gastrointestinalnog trakta",
pages = "888-879",
number = "8-9",
volume = "71",
doi = "10.2298/JSC0609879C"
}

Ćirković-Veličković, T., Polović, N., Gavrović-Jankulović, M., Burazer, L., Đergović-Petrović, D., Vučković, O., Drobnjak, O., Šporčić, Z., Atanasković-Marković, M.,& Jankov, R.. (2006). Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 71(8-9), 879-888.
https://doi.org/10.2298/JSC0609879C

Ćirković-Veličković T, Polović N, Gavrović-Jankulović M, Burazer L, Đergović-Petrović D, Vučković O, Drobnjak O, Šporčić Z, Atanasković-Marković M, Jankov R. Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract. in Journal of the Serbian Chemical Society. 2006;71(8-9):879-888.
doi:10.2298/JSC0609879C .

Ćirković-Veličković, Tanja, Polović, Natalija, Gavrović-Jankulović, Marija, Burazer, Lidija, Đergović-Petrović, Danica, Vučković, Olga, Drobnjak, Olika, Šporčić, Zorica, Atanasković-Marković, Marina, Jankov, Ratko, "Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract" in Journal of the Serbian Chemical Society, 71, no. 8-9 (2006):879-888,
https://doi.org/10.2298/JSC0609879C . .

TY  - JOUR
AU  - Atanasković-Marković, Marina
AU  - Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Vučković, Olga
AU  - Nestorovic, Branimir
PY  - 2005
UR  - http://intor.torlakinstitut.com/handle/123456789/203
AB  - Penicillins and cephalosporins are the most important betalactams inducing IgE-mediated reactions. The safety of administering cephalosporins to penicillin-allergic children is a particular problem, because cephalosporin allergenic determinants have not been properly identified. A study was undertaken to evaluate the frequency of anaphylactic reactions to cephalosporins and penicillins and their cross-reactivity in a pediatric population. A prospective survey was conducted in a group of 1170 children with suspected immediate allergic reactions to cephalosporins and/or penicillins, which were examined during a period of 8 yr. In vivo (skin tests and challenges) and in vitro tests (for specific IgE) were performed with standard concentration of penicillins and cephalosporins. When 1170 children with a clinical history of allergy to penicillins and/or cephalosporins were tested in vivo for immediate hypersensitivity to betalactams, 58.3% cases overall were found to be skin or challenge test positive. Among them, 94.4% patients were positive to penicillins and 35.3% to cephalosporins. The frequency of positive reactions in the in vivo testing was in the range from 36.4% to 88.1% for penicillins and from 0.3% to 29.2% for cephalosporins. However, 31.5% of the penicillin allergic children cross-reacted to some cephalosporin. If a child was allergic to a cephalosporin, the frequency of positive reactions to penicillin was 84.2%. The cross-reactivity between cephalosporins and penicillins varied between 0.3% and 23.9%. The cross-reactivity among different generations of cephalosporins varied between 0% and 68.8%, being the highest for first and second-generation cephalosporins and 0% for third generation cephalosporins. The frequency of immediate allergic reactions to cephalosporins is considerably lower compared to penicillins, and the degree of cross-reactivity between cephalosporins and penicillins depends on the generation of cephalosporins, being higher with earlier generation cephalosporins. The cross-reactivity among cephalosporins is lower compared to cross-reactivity between penicillins and cephalosporins.
PB  - Blackwell Munksgaard
T2  - Pediatric Allergy and Immunology
T1  - Immediate allergic reactions to cephalosporins and penicillins and their cross-reactivity in children
EP  - 347
IS  - 4
SP  - 341
VL  - 16
DO  - 10.1111/j.1399-3038.2005.00280.x
ER  - 

@article{
author = "Atanasković-Marković, Marina and Veličković, Tanja and Gavrović-Jankulović, Marija and Vučković, Olga and Nestorovic, Branimir",
year = "2005",
abstract = "Penicillins and cephalosporins are the most important betalactams inducing IgE-mediated reactions. The safety of administering cephalosporins to penicillin-allergic children is a particular problem, because cephalosporin allergenic determinants have not been properly identified. A study was undertaken to evaluate the frequency of anaphylactic reactions to cephalosporins and penicillins and their cross-reactivity in a pediatric population. A prospective survey was conducted in a group of 1170 children with suspected immediate allergic reactions to cephalosporins and/or penicillins, which were examined during a period of 8 yr. In vivo (skin tests and challenges) and in vitro tests (for specific IgE) were performed with standard concentration of penicillins and cephalosporins. When 1170 children with a clinical history of allergy to penicillins and/or cephalosporins were tested in vivo for immediate hypersensitivity to betalactams, 58.3% cases overall were found to be skin or challenge test positive. Among them, 94.4% patients were positive to penicillins and 35.3% to cephalosporins. The frequency of positive reactions in the in vivo testing was in the range from 36.4% to 88.1% for penicillins and from 0.3% to 29.2% for cephalosporins. However, 31.5% of the penicillin allergic children cross-reacted to some cephalosporin. If a child was allergic to a cephalosporin, the frequency of positive reactions to penicillin was 84.2%. The cross-reactivity between cephalosporins and penicillins varied between 0.3% and 23.9%. The cross-reactivity among different generations of cephalosporins varied between 0% and 68.8%, being the highest for first and second-generation cephalosporins and 0% for third generation cephalosporins. The frequency of immediate allergic reactions to cephalosporins is considerably lower compared to penicillins, and the degree of cross-reactivity between cephalosporins and penicillins depends on the generation of cephalosporins, being higher with earlier generation cephalosporins. The cross-reactivity among cephalosporins is lower compared to cross-reactivity between penicillins and cephalosporins.",
publisher = "Blackwell Munksgaard",
journal = "Pediatric Allergy and Immunology",
title = "Immediate allergic reactions to cephalosporins and penicillins and their cross-reactivity in children",
pages = "347-341",
number = "4",
volume = "16",
doi = "10.1111/j.1399-3038.2005.00280.x"
}

Atanasković-Marković, M., Veličković, T., Gavrović-Jankulović, M., Vučković, O.,& Nestorovic, B.. (2005). Immediate allergic reactions to cephalosporins and penicillins and their cross-reactivity in children. in Pediatric Allergy and Immunology
Blackwell Munksgaard., 16(4), 341-347.
https://doi.org/10.1111/j.1399-3038.2005.00280.x

Atanasković-Marković M, Veličković T, Gavrović-Jankulović M, Vučković O, Nestorovic B. Immediate allergic reactions to cephalosporins and penicillins and their cross-reactivity in children. in Pediatric Allergy and Immunology. 2005;16(4):341-347.
doi:10.1111/j.1399-3038.2005.00280.x .

Atanasković-Marković, Marina, Veličković, Tanja, Gavrović-Jankulović, Marija, Vučković, Olga, Nestorovic, Branimir, "Immediate allergic reactions to cephalosporins and penicillins and their cross-reactivity in children" in Pediatric Allergy and Immunology, 16, no. 4 (2005):341-347,
https://doi.org/10.1111/j.1399-3038.2005.00280.x . .

TY  - JOUR
AU  - Gavrović-Jankulović, Marija
AU  - Polović, Natalija
AU  - Prisić, S.
AU  - Jankov, Ratko
AU  - Atanasković-Marković, Marina
AU  - Vučković, Olga
AU  - Ćirković-Veličković, Tanja
PY  - 2005
UR  - http://intor.torlakinstitut.com/handle/123456789/201
AB  - Food allergies, including kiwi fruit allergy, have been the subject of extensive research in the last few years. The aim of this study was to examine a possible relationship between the developmental stage of kiwi fruit and its allergenic potency. The protein and allergen patterns of kiwi fruit extracts in September, October, November and December fruit in the period from 2000-2002 were analysed. One of the factors that may contribute to the difficulties in proposing well-defined and standardized fruit extracts should also be the time of fruit harvesting. In this particular case, when the kiwi fruit was edible throughout November and December, we showed discrepancies in allergen content and potencies both in qualitative and quantitative terms. Two major allergens of kiwi fruit, Act c 1 and Act c 2, mainly accounted for the highest allergenic potential of November kiwi extract in vivo and in vitro. Not only the content of major allergens, but also the ratio of different proteins and even isoforms of the same allergen (Act c 2) change with fruit ripening. These findings should be taken into account during preparation of extracts for allergy diagnosis.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Food and Agricultural Immunology
T1  - Allergenic potency of kiwi fruit during fruit development
EP  - 128
IS  - 2
SP  - 117
VL  - 16
DO  - 10.1080/09540100500090804
ER  - 

@article{
author = "Gavrović-Jankulović, Marija and Polović, Natalija and Prisić, S. and Jankov, Ratko and Atanasković-Marković, Marina and Vučković, Olga and Ćirković-Veličković, Tanja",
year = "2005",
abstract = "Food allergies, including kiwi fruit allergy, have been the subject of extensive research in the last few years. The aim of this study was to examine a possible relationship between the developmental stage of kiwi fruit and its allergenic potency. The protein and allergen patterns of kiwi fruit extracts in September, October, November and December fruit in the period from 2000-2002 were analysed. One of the factors that may contribute to the difficulties in proposing well-defined and standardized fruit extracts should also be the time of fruit harvesting. In this particular case, when the kiwi fruit was edible throughout November and December, we showed discrepancies in allergen content and potencies both in qualitative and quantitative terms. Two major allergens of kiwi fruit, Act c 1 and Act c 2, mainly accounted for the highest allergenic potential of November kiwi extract in vivo and in vitro. Not only the content of major allergens, but also the ratio of different proteins and even isoforms of the same allergen (Act c 2) change with fruit ripening. These findings should be taken into account during preparation of extracts for allergy diagnosis.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Food and Agricultural Immunology",
title = "Allergenic potency of kiwi fruit during fruit development",
pages = "128-117",
number = "2",
volume = "16",
doi = "10.1080/09540100500090804"
}

Gavrović-Jankulović, M., Polović, N., Prisić, S., Jankov, R., Atanasković-Marković, M., Vučković, O.,& Ćirković-Veličković, T.. (2005). Allergenic potency of kiwi fruit during fruit development. in Food and Agricultural Immunology
Taylor & Francis Ltd, Abingdon., 16(2), 117-128.
https://doi.org/10.1080/09540100500090804

Gavrović-Jankulović M, Polović N, Prisić S, Jankov R, Atanasković-Marković M, Vučković O, Ćirković-Veličković T. Allergenic potency of kiwi fruit during fruit development. in Food and Agricultural Immunology. 2005;16(2):117-128.
doi:10.1080/09540100500090804 .

Gavrović-Jankulović, Marija, Polović, Natalija, Prisić, S., Jankov, Ratko, Atanasković-Marković, Marina, Vučković, Olga, Ćirković-Veličković, Tanja, "Allergenic potency of kiwi fruit during fruit development" in Food and Agricultural Immunology, 16, no. 2 (2005):117-128,
https://doi.org/10.1080/09540100500090804 . .

TY  - JOUR
AU  - Polović, Natalija
AU  - Ćirković-Veličković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Burazer, Lidija
AU  - Đergović-Petrović, Danica
AU  - Vučković, Olga
AU  - Jankov, Ratko
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/184
AB  - This study considers the influence of exposure to simulated gastrointestinal conditions (saliva, gut, intestine and acidic conditions of the gut) on IgG binding of unmodified allergens and three types of LMW allergoids of Artemisia vulgaris pollen extract obtained by means of potassium cyanate succinic and maleic anhydride. It also concerns the optimization of a self-developed ELISA assay for comparison of the specific IgG binding of mugwort pollen extract and modified mugwort pollen derivatives. The ELISA was conducted with a mugwort pollen extract coupled to the plate, using the sera from 12 mugwort- pollen allergic patients. The exposure to saliva fluid for 2 min did not influence the IgG binding properties of allergens and allergoids. Exposure of mugwort pollen allergens and LMW allergoids to the acidic conditions of the gut did not dramatically change their IgG binding properties. By exposing mugwort pollen extract and LMW derivatives to the SGF conditions for 1 h, the percent of IgG binding epitopes was reduced to a half of its starting value in the extract and to about 30%in all the allergoid samples. After prolonged exposure only the carbamyl derivative showed reduced IgG binding. Changes of the IgG binding potential of all four samples after exposure in SIF followed a similar pattern.
AB  - Predmet ovog rada je ispitivanje promena IgG vezivanja nemodifikovanih alergena polena A. vulgaris i tri tipa alergoida malih molekulskih masa dobijenih tretmanom sa kalijum-cijanatom, i anhidridima ćilibarne i meleinske posle izlaganja uslovima gastrointestinalnog trakta (saliva, želudac, tanko crevo i kisela sredina želudačnog soka) i optimizacija domaćeg ELISA testa za određivanje IgG vezivanja alergena i alergoida polena pelina.UELISA testu je za pločicu kuplovan ekstrakt polena pelina i korišćeni su serumi 12 pacijenata alergičnih na pelin. Izlaganje salivi u trajanju od 2 minuta ne utiče na IgG vezujuće osobine alergena i alergoida. Izlaganje kiseloj sredini želudačnog soka značajno ne utiče na IgG vezujuće osobine alergena i alergoida polena pelina. Posle izlaganja simuliranom želudačnom soku alergena i derivata u trajanju od 1 sata, procenat IgG vezujućih epitopa u nemodifikovanom uzorku se smanjuje na polovinu početne vrednosti i na oko 30 % kod sva tri derivata. Jedino se kod karbamil-derivata % IgG vezivanja dodatno smanjuje sa produžavanjem izlaganja SGF-u.Promene u IgG vezujućem potencijalu sva 4 uzorka posle izlaganja simuliranim uslovima tankog creva prate sličan obrazac.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest
T1  - Igg vezivanje alergena i alergoida polena pelina prethodno izloženih simuliranim uslovima gastrointestinalnog trakta mereno domaćim elisa testom
EP  - 540
IS  - 7
SP  - 533
VL  - 69
DO  - 10.2298/JSC0407533P
ER  - 

@article{
author = "Polović, Natalija and Ćirković-Veličković, Tanja and Gavrović-Jankulović, Marija and Burazer, Lidija and Đergović-Petrović, Danica and Vučković, Olga and Jankov, Ratko",
year = "2004",
abstract = "This study considers the influence of exposure to simulated gastrointestinal conditions (saliva, gut, intestine and acidic conditions of the gut) on IgG binding of unmodified allergens and three types of LMW allergoids of Artemisia vulgaris pollen extract obtained by means of potassium cyanate succinic and maleic anhydride. It also concerns the optimization of a self-developed ELISA assay for comparison of the specific IgG binding of mugwort pollen extract and modified mugwort pollen derivatives. The ELISA was conducted with a mugwort pollen extract coupled to the plate, using the sera from 12 mugwort- pollen allergic patients. The exposure to saliva fluid for 2 min did not influence the IgG binding properties of allergens and allergoids. Exposure of mugwort pollen allergens and LMW allergoids to the acidic conditions of the gut did not dramatically change their IgG binding properties. By exposing mugwort pollen extract and LMW derivatives to the SGF conditions for 1 h, the percent of IgG binding epitopes was reduced to a half of its starting value in the extract and to about 30%in all the allergoid samples. After prolonged exposure only the carbamyl derivative showed reduced IgG binding. Changes of the IgG binding potential of all four samples after exposure in SIF followed a similar pattern., Predmet ovog rada je ispitivanje promena IgG vezivanja nemodifikovanih alergena polena A. vulgaris i tri tipa alergoida malih molekulskih masa dobijenih tretmanom sa kalijum-cijanatom, i anhidridima ćilibarne i meleinske posle izlaganja uslovima gastrointestinalnog trakta (saliva, želudac, tanko crevo i kisela sredina želudačnog soka) i optimizacija domaćeg ELISA testa za određivanje IgG vezivanja alergena i alergoida polena pelina.UELISA testu je za pločicu kuplovan ekstrakt polena pelina i korišćeni su serumi 12 pacijenata alergičnih na pelin. Izlaganje salivi u trajanju od 2 minuta ne utiče na IgG vezujuće osobine alergena i alergoida. Izlaganje kiseloj sredini želudačnog soka značajno ne utiče na IgG vezujuće osobine alergena i alergoida polena pelina. Posle izlaganja simuliranom želudačnom soku alergena i derivata u trajanju od 1 sata, procenat IgG vezujućih epitopa u nemodifikovanom uzorku se smanjuje na polovinu početne vrednosti i na oko 30 % kod sva tri derivata. Jedino se kod karbamil-derivata % IgG vezivanja dodatno smanjuje sa produžavanjem izlaganja SGF-u.Promene u IgG vezujućem potencijalu sva 4 uzorka posle izlaganja simuliranim uslovima tankog creva prate sličan obrazac.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest, Igg vezivanje alergena i alergoida polena pelina prethodno izloženih simuliranim uslovima gastrointestinalnog trakta mereno domaćim elisa testom",
pages = "540-533",
number = "7",
volume = "69",
doi = "10.2298/JSC0407533P"
}

Polović, N., Ćirković-Veličković, T., Gavrović-Jankulović, M., Burazer, L., Đergović-Petrović, D., Vučković, O.,& Jankov, R.. (2004). IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 69(7), 533-540.
https://doi.org/10.2298/JSC0407533P

Polović N, Ćirković-Veličković T, Gavrović-Jankulović M, Burazer L, Đergović-Petrović D, Vučković O, Jankov R. IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest. in Journal of the Serbian Chemical Society. 2004;69(7):533-540.
doi:10.2298/JSC0407533P .

Polović, Natalija, Ćirković-Veličković, Tanja, Gavrović-Jankulović, Marija, Burazer, Lidija, Đergović-Petrović, Danica, Vučković, Olga, Jankov, Ratko, "IgG binding of mugwort pollen allergens and allergoids exposed to simulated gastrointestinal conditions measured by a self-developed ELISAtest" in Journal of the Serbian Chemical Society, 69, no. 7 (2004):533-540,
https://doi.org/10.2298/JSC0407533P . .

TY  - JOUR
AU  - Ćirković, Tanja
AU  - Gavrović-Jankulović, Marija
AU  - Prisić, S.
AU  - Jankov, Ratko
AU  - Burazer, Lidija
AU  - Vučković, Olga
AU  - Šporčić, Zorica
AU  - Paranos, Svetlana
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/151
AB  - Background: Reaction of epsilon-amino groups of lysine with potassium cyanate, maleic, or succinic anhydride leads to allergoids of low molecular weight. No study has been performed to compare their properties and investigate the influence of a residual group on allergenicity and human IgE- and IgG-binding of these derivatives. Methods: Allergoids of a pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, and succinic and maleic anhydride. Biochemical properties were investigated by determination of amino groups, enzyme activity, isoelectric focusing IEF and SDS-PAGE. IgE- and IgG-binding was determined using immunoblots and ELISA inhibition. Allergenicity was investigated by skin prick tests (SPT) on a group of 52 patients, of which 6 were control subjects, 30 were patients with no previous immunotherapy (IT), and 16 were patients undergoing immunotherapy. Results: The same degree of amino-group modification (more than 85%), residual enzyme activity (less then 15%), IEF, and SDS-PAGE pattern were noted. In the immunoblots of IgE-binding, there was more pronounced reduction in the succinyl and maleyl derivatives than in the carbamyl one. IgG-binding was less affected by carbamylation than by acid anhydride modification. The SPT showed that the succinylated derivative had the most reduced allergenicity (98% showed a reduced wheal diameter when tested with the succinyl derivative, 87% with the maleyl allergoid, and 83% with the carbamyl allergoid). The most significant difference among allergoids could be seen in the group of patients with high skin reactivity (83% of patients showed no reaction to the succinyl derivative when compared to the value of 28% for the carbamyl derivative or 22% for the maleyl derivative). Conclusions: According to our results, all three modification procedures yielded allergoids with a similar extent of modification. No single biochemical parameter investigated in the study could predict the degree of reduced allergenicity in vivo. The most reduced allergenicity was seen in the succinyl derivative while the preservation of IgG binding epitopes was of the highest degree for the carbamyl derivative.
PB  - Wiley, Hoboken
T2  - Allergy
T1  - The influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding properties
EP  - 1020
IS  - 11
SP  - 1013
VL  - 57
DO  - 10.1034/j.1398-9995.2002.23739.x
ER  - 

@article{
author = "Ćirković, Tanja and Gavrović-Jankulović, Marija and Prisić, S. and Jankov, Ratko and Burazer, Lidija and Vučković, Olga and Šporčić, Zorica and Paranos, Svetlana",
year = "2002",
abstract = "Background: Reaction of epsilon-amino groups of lysine with potassium cyanate, maleic, or succinic anhydride leads to allergoids of low molecular weight. No study has been performed to compare their properties and investigate the influence of a residual group on allergenicity and human IgE- and IgG-binding of these derivatives. Methods: Allergoids of a pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, and succinic and maleic anhydride. Biochemical properties were investigated by determination of amino groups, enzyme activity, isoelectric focusing IEF and SDS-PAGE. IgE- and IgG-binding was determined using immunoblots and ELISA inhibition. Allergenicity was investigated by skin prick tests (SPT) on a group of 52 patients, of which 6 were control subjects, 30 were patients with no previous immunotherapy (IT), and 16 were patients undergoing immunotherapy. Results: The same degree of amino-group modification (more than 85%), residual enzyme activity (less then 15%), IEF, and SDS-PAGE pattern were noted. In the immunoblots of IgE-binding, there was more pronounced reduction in the succinyl and maleyl derivatives than in the carbamyl one. IgG-binding was less affected by carbamylation than by acid anhydride modification. The SPT showed that the succinylated derivative had the most reduced allergenicity (98% showed a reduced wheal diameter when tested with the succinyl derivative, 87% with the maleyl allergoid, and 83% with the carbamyl allergoid). The most significant difference among allergoids could be seen in the group of patients with high skin reactivity (83% of patients showed no reaction to the succinyl derivative when compared to the value of 28% for the carbamyl derivative or 22% for the maleyl derivative). Conclusions: According to our results, all three modification procedures yielded allergoids with a similar extent of modification. No single biochemical parameter investigated in the study could predict the degree of reduced allergenicity in vivo. The most reduced allergenicity was seen in the succinyl derivative while the preservation of IgG binding epitopes was of the highest degree for the carbamyl derivative.",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "The influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding properties",
pages = "1020-1013",
number = "11",
volume = "57",
doi = "10.1034/j.1398-9995.2002.23739.x"
}

Ćirković, T., Gavrović-Jankulović, M., Prisić, S., Jankov, R., Burazer, L., Vučković, O., Šporčić, Z.,& Paranos, S.. (2002). The influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding properties. in Allergy
Wiley, Hoboken., 57(11), 1013-1020.
https://doi.org/10.1034/j.1398-9995.2002.23739.x

Ćirković T, Gavrović-Jankulović M, Prisić S, Jankov R, Burazer L, Vučković O, Šporčić Z, Paranos S. The influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding properties. in Allergy. 2002;57(11):1013-1020.
doi:10.1034/j.1398-9995.2002.23739.x .

Ćirković, Tanja, Gavrović-Jankulović, Marija, Prisić, S., Jankov, Ratko, Burazer, Lidija, Vučković, Olga, Šporčić, Zorica, Paranos, Svetlana, "The influence of a residual group in low-molecular-weight allergoids of Artemisia vulgaris pollen on their allergenicity, IgE- and IgG-binding properties" in Allergy, 57, no. 11 (2002):1013-1020,
https://doi.org/10.1034/j.1398-9995.2002.23739.x . .