TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/449
AB  - We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats
EP  - 19
IS  - 1
SP  - 1
VL  - 62
DO  - 10.1556/AMicr.62.2015.1.1
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2015",
abstract = "We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats",
pages = "19-1",
number = "1",
volume = "62",
doi = "10.1556/AMicr.62.2015.1.1"
}

Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2015). Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Zrt, Budapest., 62(1), 1-19.
https://doi.org/10.1556/AMicr.62.2015.1.1

Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica. 2015;62(1):1-19.
doi:10.1556/AMicr.62.2015.1.1 .

Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats" in Acta Microbiologica et Immunologica Hungarica, 62, no. 1 (2015):1-19,
https://doi.org/10.1556/AMicr.62.2015.1.1 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/386
AB  - We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Strain differences in the humoral immune response to commensal bacterial antigens in rats
EP  - 288
IS  - 3
SP  - 271
VL  - 60
DO  - 10.1556/AMicr.60.2013.3.4
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2013",
abstract = "We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Strain differences in the humoral immune response to commensal bacterial antigens in rats",
pages = "288-271",
number = "3",
volume = "60",
doi = "10.1556/AMicr.60.2013.3.4"
}

Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2013). Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 60(3), 271-288.
https://doi.org/10.1556/AMicr.60.2013.3.4

Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica. 2013;60(3):271-288.
doi:10.1556/AMicr.60.2013.3.4 .

Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences in the humoral immune response to commensal bacterial antigens in rats" in Acta Microbiologica et Immunologica Hungarica, 60, no. 3 (2013):271-288,
https://doi.org/10.1556/AMicr.60.2013.3.4 . .

TY  - JOUR
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/326
AB  - The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection.
AB  - Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors
T1  - Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora
EP  - 132
IS  - 2-3
SP  - 119
VL  - 61
DO  - 10.2298/AVB1103119K
ER  - 

@article{
author = "Kuštrimović, Nataša and Mitić, Katarina and Dimitrijević, Mirjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava",
year = "2011",
abstract = "The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection., Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors, Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora",
pages = "132-119",
number = "2-3",
volume = "61",
doi = "10.2298/AVB1103119K"
}

Kuštrimović, N., Mitić, K., Dimitrijević, M., Vujić, V., Kovačević-Jovanović, V., Miletić, T.,& Stanojević, S.. (2011). Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 119-132.
https://doi.org/10.2298/AVB1103119K

Kuštrimović N, Mitić K, Dimitrijević M, Vujić V, Kovačević-Jovanović V, Miletić T, Stanojević S. Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd. 2011;61(2-3):119-132.
doi:10.2298/AVB1103119K .

Kuštrimović, Nataša, Mitić, Katarina, Dimitrijević, Mirjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors" in Acta veterinaria - Beograd, 61, no. 2-3 (2011):119-132,
https://doi.org/10.2298/AVB1103119K . .

TY  - JOUR
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Kuštrimović, Nataša
AU  - Kosec, Duško
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/311
AB  - We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity.
AB  - Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction
T1  - Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).
EP  - 121
IS  - 2-3
SP  - 105
VL  - 60
DO  - 10.2298/AVB1003105M
ER  - 

@article{
author = "Mitić, Katarina and Miletić, Tatjana and Kovačević-Jovanović, Vesna and Kuštrimović, Nataša and Kosec, Duško and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2010",
abstract = "We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity., Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction, Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).",
pages = "121-105",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003105M"
}

Mitić, K., Miletić, T., Kovačević-Jovanović, V., Kuštrimović, N., Kosec, D., Dimitrijević, M.,& Stanojević, S.. (2010). Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 105-121.
https://doi.org/10.2298/AVB1003105M

Mitić K, Miletić T, Kovačević-Jovanović V, Kuštrimović N, Kosec D, Dimitrijević M, Stanojević S. Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd. 2010;60(2-3):105-121.
doi:10.2298/AVB1003105M .

Mitić, Katarina, Miletić, Tatjana, Kovačević-Jovanović, Vesna, Kuštrimović, Nataša, Kosec, Duško, Dimitrijević, Mirjana, Stanojević, Stanislava, "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction" in Acta veterinaria - Beograd, 60, no. 2-3 (2010):105-121,
https://doi.org/10.2298/AVB1003105M . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/298
AB  - It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Regulatory Peptides
T1  - Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity
EP  - 109
IS  - 1-3
SP  - 100
VL  - 159
DO  - 10.1016/j.regpep.2009.11.002
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2010",
abstract = "It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity",
pages = "109-100",
number = "1-3",
volume = "159",
doi = "10.1016/j.regpep.2009.11.002"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2010). Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 159(1-3), 100-109.
https://doi.org/10.1016/j.regpep.2009.11.002

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides. 2010;159(1-3):100-109.
doi:10.1016/j.regpep.2009.11.002 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity" in Regulatory Peptides, 159, no. 1-3 (2010):100-109,
https://doi.org/10.1016/j.regpep.2009.11.002 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Mitić, Katarina
AU  - Stanojević, Stanislava
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/290
AB  - The importance of commensal bacteria in the immune system development and its involvement in the etiopatogenetic mechanisms of complex multifactorial and multigenic diseases is well documented. The aim of the present study was to compare the levels of hydrogen peroxide (H2O2) and nitric oxide (NO) produced by resident peritoneal macrophages from the autoimmune disease susceptible Dark Agouti (DA) rats vs. resistant Albino Oxford (AO) rat strain, under basal conditions and subsequent to in vitro stimulation with gut commensals. Following the stimulation with phorbol myristil acetate (PMA), E. coli/PMA or P. mirabilis/PMA, AO rats macrophages have produced significantly higher levels of H2O2 compared to the cells from DA rats. Strain differences in NO production were not detected under basal conditions and after the stimulation with lipopolysaccharide and P. mirabilis. However, after the in vitro stimulation with E. coli, AO rats macrophages have produced higher levels of NO compared to DA rats macrophages. Our results demonstrated that macrophages from AO rats have higher potential to produce H2O2 and NO in response to specific commensal bacteria when compared to DA rats. A possible relationship between the macrophage activity in response to commensal bacteria and the susceptibility to induction of autoimmune/inflammatory diseases in AO and DA rat strains is suggested.
AB  - Poznato je da komensalna crevna flora ima značajnu ulogu u razvoju imunskog sistema kao i u etiopatogenezi kompleksnih multifaktorijalnih i multigenetskih bolesti. Cilj ovog rada bio je da se uporedi produkcija vodonik peroksida (H2O2) i azot monoksida (NO) peritonealnih makrofaga dva inbredna soja pacova, od kojih je jedan osetljiv (Dark Agouti, DA), a drugi rezistentan (Albino Oxford, AO) na indukciju autoimunskih bolesti, kako u bazalnim uslovima tako i nakon in vitro stimulacije makrofaga sa crevnim komensalima. Nakon stimulacije sa forbol miristil acetatom (PMA), E. coli/PMA and P. mirabilis/PMA makrofage AO pacova su produkovale značajno više H2O2 u poređenju sa makrofagama DA pacova. Nisu detektovane sojne razlike u produkciji NO u bazalnim uslovima, kao ni posle stimulacije sa lipopolisaharidom i P. mirabilis. Međutim, nakon in vitro stimulacije sa E. coli makrofage AO pacova su produkovale više NO u odnosu na makrofage DA pacova. Naši rezultati su ukazali da makrofage AO pacova imaju veći potencijal za produkciju H2O2 i NO u odgovoru na specifične komensalne bakterije. Ova različita aktivnost makrofaga može biti u vezi sa različitom osetljivošću na indukciju autoimunskih/inflamatornih bolesti kod DA i AO soja pacova.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria
T1  - Produkcija H2O2 i NO peritonealnih makrofaga pacova u odgovoru na crevne komensalne bakterije
EP  - 122
IS  - 2-3
SP  - 111
VL  - 59
DO  - 10.2298/AVB0903111K
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Mitić, Katarina and Stanojević, Stanislava and Miletić, Tatjana and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2009",
abstract = "The importance of commensal bacteria in the immune system development and its involvement in the etiopatogenetic mechanisms of complex multifactorial and multigenic diseases is well documented. The aim of the present study was to compare the levels of hydrogen peroxide (H2O2) and nitric oxide (NO) produced by resident peritoneal macrophages from the autoimmune disease susceptible Dark Agouti (DA) rats vs. resistant Albino Oxford (AO) rat strain, under basal conditions and subsequent to in vitro stimulation with gut commensals. Following the stimulation with phorbol myristil acetate (PMA), E. coli/PMA or P. mirabilis/PMA, AO rats macrophages have produced significantly higher levels of H2O2 compared to the cells from DA rats. Strain differences in NO production were not detected under basal conditions and after the stimulation with lipopolysaccharide and P. mirabilis. However, after the in vitro stimulation with E. coli, AO rats macrophages have produced higher levels of NO compared to DA rats macrophages. Our results demonstrated that macrophages from AO rats have higher potential to produce H2O2 and NO in response to specific commensal bacteria when compared to DA rats. A possible relationship between the macrophage activity in response to commensal bacteria and the susceptibility to induction of autoimmune/inflammatory diseases in AO and DA rat strains is suggested., Poznato je da komensalna crevna flora ima značajnu ulogu u razvoju imunskog sistema kao i u etiopatogenezi kompleksnih multifaktorijalnih i multigenetskih bolesti. Cilj ovog rada bio je da se uporedi produkcija vodonik peroksida (H2O2) i azot monoksida (NO) peritonealnih makrofaga dva inbredna soja pacova, od kojih je jedan osetljiv (Dark Agouti, DA), a drugi rezistentan (Albino Oxford, AO) na indukciju autoimunskih bolesti, kako u bazalnim uslovima tako i nakon in vitro stimulacije makrofaga sa crevnim komensalima. Nakon stimulacije sa forbol miristil acetatom (PMA), E. coli/PMA and P. mirabilis/PMA makrofage AO pacova su produkovale značajno više H2O2 u poređenju sa makrofagama DA pacova. Nisu detektovane sojne razlike u produkciji NO u bazalnim uslovima, kao ni posle stimulacije sa lipopolisaharidom i P. mirabilis. Međutim, nakon in vitro stimulacije sa E. coli makrofage AO pacova su produkovale više NO u odnosu na makrofage DA pacova. Naši rezultati su ukazali da makrofage AO pacova imaju veći potencijal za produkciju H2O2 i NO u odgovoru na specifične komensalne bakterije. Ova različita aktivnost makrofaga može biti u vezi sa različitom osetljivošću na indukciju autoimunskih/inflamatornih bolesti kod DA i AO soja pacova.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria, Produkcija H2O2 i NO peritonealnih makrofaga pacova u odgovoru na crevne komensalne bakterije",
pages = "122-111",
number = "2-3",
volume = "59",
doi = "10.2298/AVB0903111K"
}

Kovačević-Jovanović, V., Mitić, K., Stanojević, S., Miletić, T., Vujić, V.,& Dimitrijević, M.. (2009). Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 111-122.
https://doi.org/10.2298/AVB0903111K

Kovačević-Jovanović V, Mitić K, Stanojević S, Miletić T, Vujić V, Dimitrijević M. Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria. in Acta veterinaria - Beograd. 2009;59(2-3):111-122.
doi:10.2298/AVB0903111K .

Kovačević-Jovanović, Vesna, Mitić, Katarina, Stanojević, Stanislava, Miletić, Tatjana, Vujić, Vesna, Dimitrijević, Mirjana, "Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria" in Acta veterinaria - Beograd, 59, no. 2-3 (2009):111-122,
https://doi.org/10.2298/AVB0903111K . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/260
AB  - Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide beta-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or beta-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of beta-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H2O2 release, neither directly nor indirectly by changing macrophage response to corticosterone and beta-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H2O2 production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and beta-endorphin in vitro. Copyright (C) 2008 S. Karger AG, Basel.
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress
EP  - 116
IS  - 2
SP  - 108
VL  - 15
DO  - 10.1159/000148193
ER  - 

@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Miletić, Tatjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2008",
abstract = "Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide beta-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or beta-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of beta-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H2O2 release, neither directly nor indirectly by changing macrophage response to corticosterone and beta-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H2O2 production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and beta-endorphin in vitro. Copyright (C) 2008 S. Karger AG, Basel.",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress",
pages = "116-108",
number = "2",
volume = "15",
doi = "10.1159/000148193"
}

Stanojević, S., Kuštrimović, N., Mitić, K., Miletić, T., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2008). The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress. in Neuroimmunomodulation
Karger, Basel., 15(2), 108-116.
https://doi.org/10.1159/000148193

Stanojević S, Kuštrimović N, Mitić K, Miletić T, Vujić V, Kovačević-Jovanović V, Dimitrijević M. The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress. in Neuroimmunomodulation. 2008;15(2):108-116.
doi:10.1159/000148193 .

Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Miletić, Tatjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress" in Neuroimmunomodulation, 15, no. 2 (2008):108-116,
https://doi.org/10.1159/000148193 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/259
AB  - We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.
PB  - Churchill Livingstone, Edinburgh
T2  - Neuropeptides
T1  - Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors
EP  - 158
IS  - 2
SP  - 147
VL  - 42
DO  - 10.1016/j.npep.2007.12.004
ER  - 

@article{
author = "Stanojević, Stanislava and Vujić, Vesna and Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Dimitrijević, Mirjana",
year = "2008",
abstract = "We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Neuropeptides",
title = "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors",
pages = "158-147",
number = "2",
volume = "42",
doi = "10.1016/j.npep.2007.12.004"
}

Stanojević, S., Vujić, V., Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Miletić, T.,& Dimitrijević, M.. (2008). Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides
Churchill Livingstone, Edinburgh., 42(2), 147-158.
https://doi.org/10.1016/j.npep.2007.12.004

Stanojević S, Vujić V, Mitić K, Kuštrimović N, Kovačević-Jovanović V, Miletić T, Dimitrijević M. Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides. 2008;42(2):147-158.
doi:10.1016/j.npep.2007.12.004 .

Stanojević, Stanislava, Vujić, Vesna, Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Dimitrijević, Mirjana, "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors" in Neuropeptides, 42, no. 2 (2008):147-158,
https://doi.org/10.1016/j.npep.2007.12.004 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/243
AB  - Neuropeptide Y (NPY)-induced modulation of the immune and inflammatory responses is regulated by tissue-specific expression of different receptor subtypes (Y1-Y6) and the activity of the enzyme dipeptidyl peptidase 4 (DP4, CD26) which terminates the action of NPY on Y1 receptor subtype. The present study investigated the age-dependent effect of NPY on inflammatory paw edema and macrophage nitric oxide production in Dark Agouti rats exhibiting a high-plasma DP4 activity, as acknowledged earlier. The results showed that NPY suppressed paw edema in adult and aged, but not in young rats. Furthermore, plasma DP4 activity decreased, while macrophage DP4 activity, as well as macrophage CD26 expression increased with aging. The use of NPY-related peptides and Y receptor-specific antagonists revealed that anti-inflammatory effect of NPY is mediated via Y1 and Y5 receptors. NPY-induced suppression of paw edema in young rats following inhibition of DP4 additionally emphasized the role for Y1 receptor in the anti-inflammatory action of NPY. In contrast to the in vivo situation, NPY stimulated macrophage nitric oxide production in vitro only in young rats, and this effect was mediated via Y1 and Y2 receptors. It can be concluded that age-dependant modulation of inflammatory reactions by NPY is determined by plasma, but not macrophage DP4 activity at different ages. (c) 2008 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age
EP  - 2187
IS  - 12
SP  - 2179
VL  - 29
DO  - 10.1016/j.peptides.2008.08.017
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2008",
abstract = "Neuropeptide Y (NPY)-induced modulation of the immune and inflammatory responses is regulated by tissue-specific expression of different receptor subtypes (Y1-Y6) and the activity of the enzyme dipeptidyl peptidase 4 (DP4, CD26) which terminates the action of NPY on Y1 receptor subtype. The present study investigated the age-dependent effect of NPY on inflammatory paw edema and macrophage nitric oxide production in Dark Agouti rats exhibiting a high-plasma DP4 activity, as acknowledged earlier. The results showed that NPY suppressed paw edema in adult and aged, but not in young rats. Furthermore, plasma DP4 activity decreased, while macrophage DP4 activity, as well as macrophage CD26 expression increased with aging. The use of NPY-related peptides and Y receptor-specific antagonists revealed that anti-inflammatory effect of NPY is mediated via Y1 and Y5 receptors. NPY-induced suppression of paw edema in young rats following inhibition of DP4 additionally emphasized the role for Y1 receptor in the anti-inflammatory action of NPY. In contrast to the in vivo situation, NPY stimulated macrophage nitric oxide production in vitro only in young rats, and this effect was mediated via Y1 and Y2 receptors. It can be concluded that age-dependant modulation of inflammatory reactions by NPY is determined by plasma, but not macrophage DP4 activity at different ages. (c) 2008 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age",
pages = "2187-2179",
number = "12",
volume = "29",
doi = "10.1016/j.peptides.2008.08.017"
}

Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2008). The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age. in Peptides
Elsevier Science Inc, New York., 29(12), 2179-2187.
https://doi.org/10.1016/j.peptides.2008.08.017

Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age. in Peptides. 2008;29(12):2179-2187.
doi:10.1016/j.peptides.2008.08.017 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age" in Peptides, 29, no. 12 (2008):2179-2187,
https://doi.org/10.1016/j.peptides.2008.08.017 . .

TY  - JOUR
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Lazarević-Macanović, Miriana
AU  - Dimitrijević, Mirjana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/229
AB  - There is extensive evidence for the critical role of reactive oxygen species (ROS) and nitric oxide (NO) produced by phagocytes in development of inflammatory processes and pathogenesis of numerous diseases, including rheumatoid arthritis (RA). Apart from their function as mediators of inflammation and tissue damage, recent research supports their role as signaling and regulatory molecules. In the present study we have investigated the production of ROS and NO over the course of adjuvant arthritis (AA) and oil-induced arthritis (OIA), by resident peritoneal macrophages of two rat strains: Dark Agouti (DA), susceptible, and Albino Oxford (AO), resistant to induction of AA and OIA. We have compared levels of ROS and NO produced by susceptible vs. resistant rat strain, and investigated their relevancy for arthritis development and severity. In addition, we have stimulated macrophages in vitro with Mycobacterium bovis BCG, and two heat shock proteins (HSP): endogenous HSP47 and mycobacterial HSP71 (rnHSP71). Our results suggest a possible contribution of increased ROS production to arthritis resistance of AO rats. The ROS production in AO rats is potentiated by endogenous HSP47, but not with mycobacterial cell and mHSP71, suggesting HSP47 participates in AA control. We have found no fundamental relationship between the magnitude of NO production and AA and OIA susceptibility and severity, suggesting that NO has no effector role in AA and OIA. Our results advocate a regulatory type action of NO molecule aught be more significant in arthritis development. (c) 2006 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Munich
T2  - Immunobiology
T1  - Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats
EP  - 105
IS  - 2
SP  - 95
VL  - 212
DO  - 10.1016/j.imbio.2006.11.012
ER  - 

@article{
author = "Miletić, Tatjana and Kovačević-Jovanović, Vesna and Vujić, Vesna and Stanojević, Stanislava and Mitić, Katarina and Lazarević-Macanović, Miriana and Dimitrijević, Mirjana",
year = "2007",
abstract = "There is extensive evidence for the critical role of reactive oxygen species (ROS) and nitric oxide (NO) produced by phagocytes in development of inflammatory processes and pathogenesis of numerous diseases, including rheumatoid arthritis (RA). Apart from their function as mediators of inflammation and tissue damage, recent research supports their role as signaling and regulatory molecules. In the present study we have investigated the production of ROS and NO over the course of adjuvant arthritis (AA) and oil-induced arthritis (OIA), by resident peritoneal macrophages of two rat strains: Dark Agouti (DA), susceptible, and Albino Oxford (AO), resistant to induction of AA and OIA. We have compared levels of ROS and NO produced by susceptible vs. resistant rat strain, and investigated their relevancy for arthritis development and severity. In addition, we have stimulated macrophages in vitro with Mycobacterium bovis BCG, and two heat shock proteins (HSP): endogenous HSP47 and mycobacterial HSP71 (rnHSP71). Our results suggest a possible contribution of increased ROS production to arthritis resistance of AO rats. The ROS production in AO rats is potentiated by endogenous HSP47, but not with mycobacterial cell and mHSP71, suggesting HSP47 participates in AA control. We have found no fundamental relationship between the magnitude of NO production and AA and OIA susceptibility and severity, suggesting that NO has no effector role in AA and OIA. Our results advocate a regulatory type action of NO molecule aught be more significant in arthritis development. (c) 2006 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Munich",
journal = "Immunobiology",
title = "Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats",
pages = "105-95",
number = "2",
volume = "212",
doi = "10.1016/j.imbio.2006.11.012"
}

Miletić, T., Kovačević-Jovanović, V., Vujić, V., Stanojević, S., Mitić, K., Lazarević-Macanović, M.,& Dimitrijević, M.. (2007). Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats. in Immunobiology
Elsevier Gmbh, Munich., 212(2), 95-105.
https://doi.org/10.1016/j.imbio.2006.11.012

Miletić T, Kovačević-Jovanović V, Vujić V, Stanojević S, Mitić K, Lazarević-Macanović M, Dimitrijević M. Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats. in Immunobiology. 2007;212(2):95-105.
doi:10.1016/j.imbio.2006.11.012 .

Miletić, Tatjana, Kovačević-Jovanović, Vesna, Vujić, Vesna, Stanojević, Stanislava, Mitić, Katarina, Lazarević-Macanović, Miriana, Dimitrijević, Mirjana, "Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats" in Immunobiology, 212, no. 2 (2007):95-105,
https://doi.org/10.1016/j.imbio.2006.11.012 . .

TY  - JOUR
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Kosec, Duško
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/213
AB  - Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71.
PB  - Wiley, Hoboken
T2  - Scandinavian Journal of Immunology
T1  - Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats
EP  - 632
IS  - 6
SP  - 623
VL  - 64
DO  - 10.1111/j.1365-3083.2006.01852.x
ER  - 

@article{
author = "Miletić, Tatjana and Kovačević-Jovanović, Vesna and Stanojević, Stanislava and Vujić, Vesna and Kosec, Duško and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2006",
abstract = "Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71.",
publisher = "Wiley, Hoboken",
journal = "Scandinavian Journal of Immunology",
title = "Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats",
pages = "632-623",
number = "6",
volume = "64",
doi = "10.1111/j.1365-3083.2006.01852.x"
}

Miletić, T., Kovačević-Jovanović, V., Stanojević, S., Vujić, V., Kosec, D., Mitić, K.,& Dimitrijević, M.. (2006). Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats. in Scandinavian Journal of Immunology
Wiley, Hoboken., 64(6), 623-632.
https://doi.org/10.1111/j.1365-3083.2006.01852.x

Miletić T, Kovačević-Jovanović V, Stanojević S, Vujić V, Kosec D, Mitić K, Dimitrijević M. Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats. in Scandinavian Journal of Immunology. 2006;64(6):623-632.
doi:10.1111/j.1365-3083.2006.01852.x .

Miletić, Tatjana, Kovačević-Jovanović, Vesna, Stanojević, Stanislava, Vujić, Vesna, Kosec, Duško, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats" in Scandinavian Journal of Immunology, 64, no. 6 (2006):623-632,
https://doi.org/10.1111/j.1365-3083.2006.01852.x . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Radulović, Jelena
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/188
AB  - The objective of the present study was to explore whether the suppressive effect of electric stress (ES) on the immune response in rats was limited to the particular antigen given concomitantly with ES. Therefore, the influence of simultaneous exposure to stress and immunization with an unrelated antigen (keyhole limpet hemocyanin, KLH) on the humoral immune response to bovine serum albumin (BSA) was investigated. Specific anti-KLH antibody levels were also determined in rats exposed to ES and concomitantly immunized with BSA. Five daily sessions of ES or immunization with KLH 2 weeks prior to immunization with BSA did not influence the secondary humoral immune response to BSA, but concomitant exposure to ES and immunization with KLH significantly decreased it. Conversely, the primary humoral immune response to KLH was suppressed by exposure of the animals to ES at the time of immunization with KLH, as well as at the time of the immunization with BSA 2 weeks later. It is suggested that the suppressive effect of ES on the humoral immune response is not specific for a certain antigen. However, the chemical and immunological characteristics of the antigens shaped the profile of stress-induced immune changes with respect to the sensitivity of the primary and secondary immune response and the duration of the effect.
AB  - Cilj rada bio je da se utvrdi da li je supresivni efekat električnog stresa (ES) na imunski odgovor specifičan za antigen kojim su pacovi imunizovani u vreme izlaganja ES. Ispitivan je uticaj istovremene primene stresa i imunizacije sa nesrodnim antigenom (keyhole limpet hemocyanin, KLH) na humoralni imunski odgovor prema goveđem serum albuminu (GSA). Takođe su određivana i specifična anti-KLH antitela u serumima pacova koji su bili istovremeno izloženi stresu i imunizovani sa GSA. Rezultati su pokazali da ni petodnevni ES, ni imunizacija sa KLH dve nedelje pre imunizacije sa GSA nisu uticali na nivo anti- GSA antitela, za razliku od istovremenog izlaganja ES i imunizacije sa GSA koji su značajno suprimirali sekundarni humoralni imunski odgovor prema GSA. Nasuprot tome, primarni humoralni imunski odgovor prema KLH je bio suprimiran u pacova koji su bili izloženi stresu tokom imunizacije sa KLH, ali i kod onih koji su bili izloženi stresu dve nedelje kasnije odnosno tokom imunizacije sa GSA. Naši rezultati ukazuju da supresivni efekat stresa na imunski odgovor nije specifičan za određen antigen, kao i da hemijske i imunološke osobine antigena značajno utiču na kvalitet promena izazvanih stresom u pogledu osetljivosti primarnog i sekundarnog imunskog odgovora na stres i trajanja efekata stresa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Antigen characteristics strongly influenced the effects of stress on the humoral immune response in the rat
T1  - Karakteristike antigena utiču na efekte stresa na humoralni imunski odgovor u pacova
EP  - 94
IS  - 2-3
SP  - 85
VL  - 54
DO  - 10.2298/AVB0403085S
ER  - 

@article{
author = "Stanojević, Stanislava and Radulović, Jelena and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2004",
abstract = "The objective of the present study was to explore whether the suppressive effect of electric stress (ES) on the immune response in rats was limited to the particular antigen given concomitantly with ES. Therefore, the influence of simultaneous exposure to stress and immunization with an unrelated antigen (keyhole limpet hemocyanin, KLH) on the humoral immune response to bovine serum albumin (BSA) was investigated. Specific anti-KLH antibody levels were also determined in rats exposed to ES and concomitantly immunized with BSA. Five daily sessions of ES or immunization with KLH 2 weeks prior to immunization with BSA did not influence the secondary humoral immune response to BSA, but concomitant exposure to ES and immunization with KLH significantly decreased it. Conversely, the primary humoral immune response to KLH was suppressed by exposure of the animals to ES at the time of immunization with KLH, as well as at the time of the immunization with BSA 2 weeks later. It is suggested that the suppressive effect of ES on the humoral immune response is not specific for a certain antigen. However, the chemical and immunological characteristics of the antigens shaped the profile of stress-induced immune changes with respect to the sensitivity of the primary and secondary immune response and the duration of the effect., Cilj rada bio je da se utvrdi da li je supresivni efekat električnog stresa (ES) na imunski odgovor specifičan za antigen kojim su pacovi imunizovani u vreme izlaganja ES. Ispitivan je uticaj istovremene primene stresa i imunizacije sa nesrodnim antigenom (keyhole limpet hemocyanin, KLH) na humoralni imunski odgovor prema goveđem serum albuminu (GSA). Takođe su određivana i specifična anti-KLH antitela u serumima pacova koji su bili istovremeno izloženi stresu i imunizovani sa GSA. Rezultati su pokazali da ni petodnevni ES, ni imunizacija sa KLH dve nedelje pre imunizacije sa GSA nisu uticali na nivo anti- GSA antitela, za razliku od istovremenog izlaganja ES i imunizacije sa GSA koji su značajno suprimirali sekundarni humoralni imunski odgovor prema GSA. Nasuprot tome, primarni humoralni imunski odgovor prema KLH je bio suprimiran u pacova koji su bili izloženi stresu tokom imunizacije sa KLH, ali i kod onih koji su bili izloženi stresu dve nedelje kasnije odnosno tokom imunizacije sa GSA. Naši rezultati ukazuju da supresivni efekat stresa na imunski odgovor nije specifičan za određen antigen, kao i da hemijske i imunološke osobine antigena značajno utiču na kvalitet promena izazvanih stresom u pogledu osetljivosti primarnog i sekundarnog imunskog odgovora na stres i trajanja efekata stresa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Antigen characteristics strongly influenced the effects of stress on the humoral immune response in the rat, Karakteristike antigena utiču na efekte stresa na humoralni imunski odgovor u pacova",
pages = "94-85",
number = "2-3",
volume = "54",
doi = "10.2298/AVB0403085S"
}

Stanojević, S., Radulović, J., Kovačević-Jovanović, V., Miletić, T., Vujić, V.,& Dimitrijević, M.. (2004). Antigen characteristics strongly influenced the effects of stress on the humoral immune response in the rat. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(2-3), 85-94.
https://doi.org/10.2298/AVB0403085S

Stanojević S, Radulović J, Kovačević-Jovanović V, Miletić T, Vujić V, Dimitrijević M. Antigen characteristics strongly influenced the effects of stress on the humoral immune response in the rat. in Acta veterinaria - Beograd. 2004;54(2-3):85-94.
doi:10.2298/AVB0403085S .

Stanojević, Stanislava, Radulović, Jelena, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić, Vesna, Dimitrijević, Mirjana, "Antigen characteristics strongly influenced the effects of stress on the humoral immune response in the rat" in Acta veterinaria - Beograd, 54, no. 2-3 (2004):85-94,
https://doi.org/10.2298/AVB0403085S . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Dimitrijević, Mirjana
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Radulović, Jelena
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/165
AB  - The effect of unpredictable, inescapable and uncontrollable electric tail shocks (ES) on the humoral immune response to bovine serum albumin (BSA) was investigated in the rat. Contributions of the procedures that accompany shock delivery, such as witnessing the ES procedure (stress witnessing, SW) and exposure to the apparatus for shock delivery (apparatus control, AC) to the changes in specific immunity induced by ES were also tested. All procedures were applied during primary and/or secondary immunization. It was demonstrated that exposure to ES during primary immunization with BSA significantly suppressed specific anti-BSA antibody production after secondary and tertiary immunization with the same antigen. Exposure to the SW procedure during primary immunization with BSA enhanced the specific antibody level after secondary immunization, while exposure to the apparatus alone did not influence the development of either the primary or secondary humoral immune response to BSA. Both ES-induced suppression and SW-induced potentiation of the humoral immune response were partially inhibited by prior treatment with the opioid receptor antagonist naloxone. Additionally, treatments with the opioid peptides methionine- and leucine-enkephalin decreased anti-BSA antibody level, mimicking to some extent the effects of ES. It is suggested that ES and endogenous opioid peptides had long-term effects on humoral immunity through mechanisms involving immunologic memory.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Stress-The International Journal on the Biology of Stress
T1  - Stress applied during primary immunization affects the secondary humoral immune response in the rat: Involvement of opioid peptides
EP  - 258
IS  - 4
SP  - 247
VL  - 6
DO  - 10.1080/1025389032000114515
ER  - 

@article{
author = "Stanojević, Stanislava and Dimitrijević, Mirjana and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić, Vesna and Radulović, Jelena",
year = "2003",
abstract = "The effect of unpredictable, inescapable and uncontrollable electric tail shocks (ES) on the humoral immune response to bovine serum albumin (BSA) was investigated in the rat. Contributions of the procedures that accompany shock delivery, such as witnessing the ES procedure (stress witnessing, SW) and exposure to the apparatus for shock delivery (apparatus control, AC) to the changes in specific immunity induced by ES were also tested. All procedures were applied during primary and/or secondary immunization. It was demonstrated that exposure to ES during primary immunization with BSA significantly suppressed specific anti-BSA antibody production after secondary and tertiary immunization with the same antigen. Exposure to the SW procedure during primary immunization with BSA enhanced the specific antibody level after secondary immunization, while exposure to the apparatus alone did not influence the development of either the primary or secondary humoral immune response to BSA. Both ES-induced suppression and SW-induced potentiation of the humoral immune response were partially inhibited by prior treatment with the opioid receptor antagonist naloxone. Additionally, treatments with the opioid peptides methionine- and leucine-enkephalin decreased anti-BSA antibody level, mimicking to some extent the effects of ES. It is suggested that ES and endogenous opioid peptides had long-term effects on humoral immunity through mechanisms involving immunologic memory.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Stress-The International Journal on the Biology of Stress",
title = "Stress applied during primary immunization affects the secondary humoral immune response in the rat: Involvement of opioid peptides",
pages = "258-247",
number = "4",
volume = "6",
doi = "10.1080/1025389032000114515"
}

Stanojević, S., Dimitrijević, M., Kovačević-Jovanović, V., Miletić, T., Vujić, V.,& Radulović, J.. (2003). Stress applied during primary immunization affects the secondary humoral immune response in the rat: Involvement of opioid peptides. in Stress-The International Journal on the Biology of Stress
Taylor & Francis Ltd, Abingdon., 6(4), 247-258.
https://doi.org/10.1080/1025389032000114515

Stanojević S, Dimitrijević M, Kovačević-Jovanović V, Miletić T, Vujić V, Radulović J. Stress applied during primary immunization affects the secondary humoral immune response in the rat: Involvement of opioid peptides. in Stress-The International Journal on the Biology of Stress. 2003;6(4):247-258.
doi:10.1080/1025389032000114515 .

Stanojević, Stanislava, Dimitrijević, Mirjana, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić, Vesna, Radulović, Jelena, "Stress applied during primary immunization affects the secondary humoral immune response in the rat: Involvement of opioid peptides" in Stress-The International Journal on the Biology of Stress, 6, no. 4 (2003):247-258,
https://doi.org/10.1080/1025389032000114515 . .

TY  - JOUR
AU  - Todorović, C.
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Laban, Olgica
AU  - Radulović, Jelena
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/160
AB  - We investigated the relationship between immunological and behavioral changes during ageing in Dark Agouti female rats. Results showed that ageing was associated with decreased exploratory behavior and increased emotionality (open field test) and decreased pain perception (writhing assay), but not with altered depression-like behavior (forced swim test). The observed behavioral changes were paralleled with decreased innate immunity in middle-aged and old rats, as revealed by reduced peroxide production of peritoneal macrophages; and decreased specific immunity, measured by the plaque-forming cell response, in old rats in comparison with young rats. Correlation analyses between behavioral and immune parameters demonstrated a significant correlation between the lines crossed in the open field test and the plaque-forming cell response. Taken together, the demonstrated age-dependent association between exploratory behavior and specific immune response suggests a senescent decline of a common neuroimmune regulatory mechanism.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Correlation between age-related changes in open field behavior and plaque forming cell response in DA female rats
EP  - 1273
IS  - 9
SP  - 1259
VL  - 113
DO  - 10.1080/00207450390212492
ER  - 

@article{
author = "Todorović, C. and Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Laban, Olgica and Radulović, Jelena",
year = "2003",
abstract = "We investigated the relationship between immunological and behavioral changes during ageing in Dark Agouti female rats. Results showed that ageing was associated with decreased exploratory behavior and increased emotionality (open field test) and decreased pain perception (writhing assay), but not with altered depression-like behavior (forced swim test). The observed behavioral changes were paralleled with decreased innate immunity in middle-aged and old rats, as revealed by reduced peroxide production of peritoneal macrophages; and decreased specific immunity, measured by the plaque-forming cell response, in old rats in comparison with young rats. Correlation analyses between behavioral and immune parameters demonstrated a significant correlation between the lines crossed in the open field test and the plaque-forming cell response. Taken together, the demonstrated age-dependent association between exploratory behavior and specific immune response suggests a senescent decline of a common neuroimmune regulatory mechanism.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Correlation between age-related changes in open field behavior and plaque forming cell response in DA female rats",
pages = "1273-1259",
number = "9",
volume = "113",
doi = "10.1080/00207450390212492"
}

Todorović, C., Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T., Laban, O.,& Radulović, J.. (2003). Correlation between age-related changes in open field behavior and plaque forming cell response in DA female rats. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 113(9), 1259-1273.
https://doi.org/10.1080/00207450390212492

Todorović C, Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Laban O, Radulović J. Correlation between age-related changes in open field behavior and plaque forming cell response in DA female rats. in International Journal of Neuroscience. 2003;113(9):1259-1273.
doi:10.1080/00207450390212492 .

Todorović, C., Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Laban, Olgica, Radulović, Jelena, "Correlation between age-related changes in open field behavior and plaque forming cell response in DA female rats" in International Journal of Neuroscience, 113, no. 9 (2003):1259-1273,
https://doi.org/10.1080/00207450390212492 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Laban, Olgica
AU  - Đurić, V.J.
AU  - Stanojević, Stanislava
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Todorović, C.
AU  - Radulović, Jelena
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/128
AB  - Previous research has suggested that behavioral traits of the histocompatible Lewis and Fischer strains of rats could be related to the difference in their susceptibility to adjuvant arthritis (AA), In the present study, the predictive value of behavioral markers in susceptibility to AA was investigated in nonhistocompatible inbred DA, Lewis, Albino Oxford (AO), and outbred Wistar strain. Behavioral profiles (open filed test and forced swim test) were determined prior to immunization with a single intradermal injection of complete Freund's adjuvant. Animals were daily scored for clinical signs of AA. The occurrence of certain behaviors and clinical indices of AA was significantly associated with strain membership. Discriminant analysis identified strain-related behavioral and illness profiles with very few overlaps among the phenotypes. Discriminant classification significantly exceeded the proportion of cases, which could have been correctly classified on the basis of chance. Open field behavior, in particular, exploration and grooming, differentiated among AA-susceptible and AA-resistant strains. Multiple regression analysis indicated that severity of AA (maximum clinical sign) can be predicted by the latency time and grooming behavior in the open field independently of strain membership. No clear distinction between AA-susceptible and AA-resistant strains was found with respect to forced swim test immobility. It was concluded that (a) strain-related genetic predisposition is important for the expression of certain behavioral traits and for susceptibility to AA and (b) open field behaviors, particularly grooming and latency, predict susceptibility to AA across different rat strains. (C) 2001 Academic Press.
PB  - Academic Press Inc, San Diego
T2  - Brain Behavior and Immunity
T1  - Behavior and severity of adjuvant arthritis in four rat strains
EP  - 265
IS  - 3
SP  - 255
VL  - 15
DO  - 10.1006/brbi.2000.0599
ER  - 

@article{
author = "Dimitrijević, Mirjana and Laban, Olgica and Đurić, V.J. and Stanojević, Stanislava and Miletić, Tatjana and Kovačević-Jovanović, Vesna and Todorović, C. and Radulović, Jelena",
year = "2001",
abstract = "Previous research has suggested that behavioral traits of the histocompatible Lewis and Fischer strains of rats could be related to the difference in their susceptibility to adjuvant arthritis (AA), In the present study, the predictive value of behavioral markers in susceptibility to AA was investigated in nonhistocompatible inbred DA, Lewis, Albino Oxford (AO), and outbred Wistar strain. Behavioral profiles (open filed test and forced swim test) were determined prior to immunization with a single intradermal injection of complete Freund's adjuvant. Animals were daily scored for clinical signs of AA. The occurrence of certain behaviors and clinical indices of AA was significantly associated with strain membership. Discriminant analysis identified strain-related behavioral and illness profiles with very few overlaps among the phenotypes. Discriminant classification significantly exceeded the proportion of cases, which could have been correctly classified on the basis of chance. Open field behavior, in particular, exploration and grooming, differentiated among AA-susceptible and AA-resistant strains. Multiple regression analysis indicated that severity of AA (maximum clinical sign) can be predicted by the latency time and grooming behavior in the open field independently of strain membership. No clear distinction between AA-susceptible and AA-resistant strains was found with respect to forced swim test immobility. It was concluded that (a) strain-related genetic predisposition is important for the expression of certain behavioral traits and for susceptibility to AA and (b) open field behaviors, particularly grooming and latency, predict susceptibility to AA across different rat strains. (C) 2001 Academic Press.",
publisher = "Academic Press Inc, San Diego",
journal = "Brain Behavior and Immunity",
title = "Behavior and severity of adjuvant arthritis in four rat strains",
pages = "265-255",
number = "3",
volume = "15",
doi = "10.1006/brbi.2000.0599"
}

Dimitrijević, M., Laban, O., Đurić, V.J., Stanojević, S., Miletić, T., Kovačević-Jovanović, V., Todorović, C.,& Radulović, J.. (2001). Behavior and severity of adjuvant arthritis in four rat strains. in Brain Behavior and Immunity
Academic Press Inc, San Diego., 15(3), 255-265.
https://doi.org/10.1006/brbi.2000.0599

Dimitrijević M, Laban O, Đurić V, Stanojević S, Miletić T, Kovačević-Jovanović V, Todorović C, Radulović J. Behavior and severity of adjuvant arthritis in four rat strains. in Brain Behavior and Immunity. 2001;15(3):255-265.
doi:10.1006/brbi.2000.0599 .

Dimitrijević, Mirjana, Laban, Olgica, Đurić, V.J., Stanojević, Stanislava, Miletić, Tatjana, Kovačević-Jovanović, Vesna, Todorović, C., Radulović, Jelena, "Behavior and severity of adjuvant arthritis in four rat strains" in Brain Behavior and Immunity, 15, no. 3 (2001):255-265,
https://doi.org/10.1006/brbi.2000.0599 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Vujić-Redžić, V.
AU  - Radulović, Jelena
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/124
AB  - We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Immunopharmacology
T1  - Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors
EP  - 262
IS  - 3
SP  - 255
VL  - 49
DO  - 10.1016/S0162-3109(00)00213-7
ER  - 

@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Vujić-Redžić, V. and Radulović, Jelena",
year = "2000",
abstract = "We have previously demonstrated that central application of leucine-enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (delta) and OP2 (kappa) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (mu) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met-Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor-selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor-binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist beta-funaltrexamine (beta-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by beta-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met-Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Immunopharmacology",
title = "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors",
pages = "262-255",
number = "3",
volume = "49",
doi = "10.1016/S0162-3109(00)00213-7"
}

Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T., Vujić-Redžić, V.,& Radulović, J.. (2000). Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology
Elsevier, Amsterdam., 49(3), 255-262.
https://doi.org/10.1016/S0162-3109(00)00213-7

Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Vujić-Redžić V, Radulović J. Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors. in Immunopharmacology. 2000;49(3):255-262.
doi:10.1016/S0162-3109(00)00213-7 .

Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Vujić-Redžić, V., Radulović, Jelena, "Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists: functional interactions of brain OP1, OP2 and OP3 receptors" in Immunopharmacology, 49, no. 3 (2000):255-262,
https://doi.org/10.1016/S0162-3109(00)00213-7 . .

TY  - JOUR
AU  - Vujić-Redžić, V.
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Radulović, Jelena
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/115
AB  - Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat
EP  - 77
IS  - 2
SP  - 70
VL  - 8
DO  - 10.1159/000026455
ER  - 

@article{
author = "Vujić-Redžić, V. and Dimitrijević, Mirjana and Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Radulović, Jelena",
year = "2000",
abstract = "Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central nervous system functions. The present study was conducted in order to compare peripheral and central effects of Met-Enk on nonspecific immunity, open field behavior and pain perception in the rat. The results showed that 0.2 mg/kg of Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated H2O2 production of peritoneal macrophages. Met-Enk-induced immunopotentiation was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and vertical locomotor activity in the open field that was reversed by i.p. administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk applied i.p. did not affect the number of writhes in the test of analgesia. Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was previously shown to be immunostimulatory, enhanced only basal H2O2 production of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H2O2 production of peritoneal macrophages under basal and stimulated conditions, respectively, Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was sufficient to modulate immune functions did not influence behavior. It may be concluded that Met-Enk modulated nonspecific immune responses and open field behavior by peripheral mechanisms. Copyright (C) 2000 S. Karger AG, Basel.",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat",
pages = "77-70",
number = "2",
volume = "8",
doi = "10.1159/000026455"
}

Vujić-Redžić, V., Dimitrijević, M., Stanojević, S., Kovačević-Jovanović, V., Miletić, T.,& Radulović, J.. (2000). Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat. in Neuroimmunomodulation
Karger, Basel., 8(2), 70-77.
https://doi.org/10.1159/000026455

Vujić-Redžić V, Dimitrijević M, Stanojević S, Kovačević-Jovanović V, Miletić T, Radulović J. Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat. in Neuroimmunomodulation. 2000;8(2):70-77.
doi:10.1159/000026455 .

Vujić-Redžić, V., Dimitrijević, Mirjana, Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Radulović, Jelena, "Peripheral effects of methionine-enkephalin on inflammatory reactions and behavior in the rat" in Neuroimmunomodulation, 8, no. 2 (2000):70-77,
https://doi.org/10.1159/000026455 . .

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Laban, Olgica
AU  - Stanojević, Stanislava
AU  - Miletić, Tatjana
AU  - Dimitrijević, Mirjana
AU  - Radulović, Jelena
PY  - 1997
UR  - http://intor.torlakinstitut.com/handle/123456789/72
AB  - The serum antibody response to ovalbumin (OA) has been investigated following intracerebroventricular (i.c.v.) and intravenous administration of antigen in the rat, under altered neuronal and immunological conditions. I.c.v. administration of antigen was far more potent in eliciting humoral immune response. Central nervous system (CNS) immunization under the conditions of disrupted blood-brain barrier decreased anti-GA antibody production. Peripheral polyclonal stimulation with Bordetella per tussis increased production of specific antibodies to i.c.v. injected antigen, while complete Freund's adjuvant had no effect on the immune response. These results suggest that CNS compartmentalization of antigen may be critical for mounting strong antibody production, and that peripheral polyclonal stimulation of the immune system may markedly contribute to the overall intensity of the immune response.
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - Changes in immunological and neuronal conditions markedly altered antibody response to intracerebroventricularly injected ovalbumin in the rat
EP  - 187
IS  - 4
SP  - 181
VL  - 4
DO  - 10.1159/000097336
ER  - 

@article{
author = "Kovačević-Jovanović, Vesna and Laban, Olgica and Stanojević, Stanislava and Miletić, Tatjana and Dimitrijević, Mirjana and Radulović, Jelena",
year = "1997",
abstract = "The serum antibody response to ovalbumin (OA) has been investigated following intracerebroventricular (i.c.v.) and intravenous administration of antigen in the rat, under altered neuronal and immunological conditions. I.c.v. administration of antigen was far more potent in eliciting humoral immune response. Central nervous system (CNS) immunization under the conditions of disrupted blood-brain barrier decreased anti-GA antibody production. Peripheral polyclonal stimulation with Bordetella per tussis increased production of specific antibodies to i.c.v. injected antigen, while complete Freund's adjuvant had no effect on the immune response. These results suggest that CNS compartmentalization of antigen may be critical for mounting strong antibody production, and that peripheral polyclonal stimulation of the immune system may markedly contribute to the overall intensity of the immune response.",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "Changes in immunological and neuronal conditions markedly altered antibody response to intracerebroventricularly injected ovalbumin in the rat",
pages = "187-181",
number = "4",
volume = "4",
doi = "10.1159/000097336"
}

Kovačević-Jovanović, V., Laban, O., Stanojević, S., Miletić, T., Dimitrijević, M.,& Radulović, J.. (1997). Changes in immunological and neuronal conditions markedly altered antibody response to intracerebroventricularly injected ovalbumin in the rat. in Neuroimmunomodulation
Karger, Basel., 4(4), 181-187.
https://doi.org/10.1159/000097336

Kovačević-Jovanović V, Laban O, Stanojević S, Miletić T, Dimitrijević M, Radulović J. Changes in immunological and neuronal conditions markedly altered antibody response to intracerebroventricularly injected ovalbumin in the rat. in Neuroimmunomodulation. 1997;4(4):181-187.
doi:10.1159/000097336 .

Kovačević-Jovanović, Vesna, Laban, Olgica, Stanojević, Stanislava, Miletić, Tatjana, Dimitrijević, Mirjana, Radulović, Jelena, "Changes in immunological and neuronal conditions markedly altered antibody response to intracerebroventricularly injected ovalbumin in the rat" in Neuroimmunomodulation, 4, no. 4 (1997):181-187,
https://doi.org/10.1159/000097336 . .