TY  - JOUR
AU  - Stojković, Aleksandra
AU  - Maslovarić, Irina
AU  - Kosanović, Dejana
AU  - Vučetić, Dušan
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/400
AB  - A small dose of the Bordetella pertussis vaccine is used as an adjuvant for the induction of experimental autoimmune encephalomyelitis (EAE) in mice. The effects of two doses of the Pertussis vaccine on clinical signs, antibody titers, and the expression of CD4 and MHC molecules in brain tissue sections of mice with EAE were examined. EAE was induced by spinal cord homogenate in Complete Freund adjuvant (CFA) in 30 of 40 C57BL/6 mice divided in groups: EAE mice with a small adjuvant dose of the Pertussis vaccine (EAE-1), EAE mice with a human dose of the Pertussis vaccine (EAE-2), EAE mice (EAE-3). None of the mice from the EAE groups progressed to severe EAE. Five mice from the EAE-2 group were found dead on the 13th day post-immunization. A significant increase of anti-MOG (myelin oligodendrocyte glycoprotein) antibodies was detected in mice with EAE compared to non-treated mice. Myelin loss and brain tissue lesions were observed in EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A high expression of MHC-II and a mild expression of MHC-I was detected in the brains of mice with EAE. No expressions were detected in intact brains. Scattered CD4-positive cells were detected in the brains of EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A small dose of the Bordetella pertussis vaccine could maintain the developed clinical signs and histological changes in mice with EAE, while higher doses led to additional adverse effects. The expression of CD4 and MHC class I and II molecules, as well as an increase in anti-MOG antibodies could be used as markers capable of monitoring the development and progression of EAE.
PB  - De Gruyter Poland Sp Zoo, Warsaw
T2  - Translational Neuroscience
T1  - Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice
EP  - 63
IS  - 1
SP  - 57
VL  - 5
DO  - 10.2478/s13380-014-0206-x
ER  - 

@article{
author = "Stojković, Aleksandra and Maslovarić, Irina and Kosanović, Dejana and Vučetić, Dušan",
year = "2014",
abstract = "A small dose of the Bordetella pertussis vaccine is used as an adjuvant for the induction of experimental autoimmune encephalomyelitis (EAE) in mice. The effects of two doses of the Pertussis vaccine on clinical signs, antibody titers, and the expression of CD4 and MHC molecules in brain tissue sections of mice with EAE were examined. EAE was induced by spinal cord homogenate in Complete Freund adjuvant (CFA) in 30 of 40 C57BL/6 mice divided in groups: EAE mice with a small adjuvant dose of the Pertussis vaccine (EAE-1), EAE mice with a human dose of the Pertussis vaccine (EAE-2), EAE mice (EAE-3). None of the mice from the EAE groups progressed to severe EAE. Five mice from the EAE-2 group were found dead on the 13th day post-immunization. A significant increase of anti-MOG (myelin oligodendrocyte glycoprotein) antibodies was detected in mice with EAE compared to non-treated mice. Myelin loss and brain tissue lesions were observed in EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A high expression of MHC-II and a mild expression of MHC-I was detected in the brains of mice with EAE. No expressions were detected in intact brains. Scattered CD4-positive cells were detected in the brains of EAE-1 and EAE-2 mice compared to EAE-3 and non-treated mice. A small dose of the Bordetella pertussis vaccine could maintain the developed clinical signs and histological changes in mice with EAE, while higher doses led to additional adverse effects. The expression of CD4 and MHC class I and II molecules, as well as an increase in anti-MOG antibodies could be used as markers capable of monitoring the development and progression of EAE.",
publisher = "De Gruyter Poland Sp Zoo, Warsaw",
journal = "Translational Neuroscience",
title = "Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice",
pages = "63-57",
number = "1",
volume = "5",
doi = "10.2478/s13380-014-0206-x"
}

Stojković, A., Maslovarić, I., Kosanović, D.,& Vučetić, D.. (2014). Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice. in Translational Neuroscience
De Gruyter Poland Sp Zoo, Warsaw., 5(1), 57-63.
https://doi.org/10.2478/s13380-014-0206-x

Stojković A, Maslovarić I, Kosanović D, Vučetić D. Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice. in Translational Neuroscience. 2014;5(1):57-63.
doi:10.2478/s13380-014-0206-x .

Stojković, Aleksandra, Maslovarić, Irina, Kosanović, Dejana, Vučetić, Dušan, "Pertussis vaccine-induced experimental autoimmune encephalomyelitis in mice" in Translational Neuroscience, 5, no. 1 (2014):57-63,
https://doi.org/10.2478/s13380-014-0206-x . .