TY  - JOUR
AU  - Radovanović, Anita M.
AU  - Mićić, Mileva
AU  - Gledić, Dušan S.
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/141
AB  - The aim of this study was to investigate the localization and distribution of sympathetic positive structures in the female gonads and oviducts of 15 30 and 60-day-oldAO rats. Histofluorescence and immunohistochemistry methods were used to determine their distribution in the ovary and oviduct, as well as for identification of dopamine and serotonin within sympathetic positive structures. Total monoamine positive structures (fibers and cells) were mainly localized under the surface epithelium, between follicles, especially surrounding groups of primary follicles and between secondary interstitial cells. The distribution of dopamine (DA) immunolabelled fibers and cells was in accordance with the distribution of total monoaminergic structures detected using histofluorescence. However, the density of structures labeled with DA antibodies was lower compared to total catecholamine positive structures detected by the fluorescence method. Delicate serotonin (5-HT) positive fibers were present mainly under the surface epithelium and in interstitial tissue between the cortex and medulla. In the hilum and medulla 5-HT positive cells were found perivascularly, while a dense population of 5-HT positive cells was found in the oviduct and mesovarium in 60-day-old animals. No corpus luteum cells were labelled using either of the two methods. Our findings corroborate observations that the monoamine supply of the ovary is not exclusively via extrinsic innervation and that intragonadal sources have an important role in their synthesis. Furthermore, the intraovarian localization of these elements suggests that monoamines exert direct or indirect effects on ovarian function.
AB  - Cilj našeg rada je bio ispitivanje lokalizacije i distribucije struktura pozitivnih na monoaminske neurotransmitere u jajnicima i jajovodima pacova. Za identifikaciju neurotransmitera u jajnicima i jajovodima 15, 30 i 60 dana starih AO pacova koristili smo histofluorescenciju (ukupni monoamini) i imunocitohemiju (dopamin i serotonin). Pozitivne monoaminergičke strukture prisutne su između folikula, naročito oko primarnih folikula, kao i između sekundarnih intersticijalnih ćelija. U mezoovarijumu i jajovodu 30 i 60 dana starih životinja zapažaju se fluorescentne ćelije i vlakna. Dopaminergične strukture su prisutne na istim mestima, ali njihova gustina je manja ako se uporede sa strukturama pozitivnim na ukupne monoamine. Retka serotonin-pozitivna vlakna zapažaju se uglavnom ispod klicinog epitela i u intersticijumu između kore i srži jajnika. Kod 30 i 60 dana starih ženki u hilusu i srži, pretežno oko krvnih sudova, nalaze se serotonin pozitivne ćelije, dok se u mezoovarijumu i jajovodu nalazi gusta populacija pozitivnih ćelija. Ni jednom od primenjenih metoda nije uočeno prisustvo monoamina u žutom telu. Naši rezultati potvrduju da neurotransmiteri u jajnicima i jajovodima ne potiču samo iz nervnih terminala, već da značajnu ulogu u njihovoj sintezi imaju intragonadalni izvori. Lokalizacija ovih elemenata pokazuje da monoaminski neurotransmiteri mogu ispoljavati direktan i!i indirektan uticaj na funkciju jajnika.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Localization of-monoamine positive structures in the ovary and oviduct of developing rats
T1  - Lokalizacija monoaminskih neurotransmitera u jajnicima i jajovodima pacova tokom polnog sazrevanja
EP  - 222
IS  - 4
SP  - 211
VL  - 52
DO  - 10.2298/AVB0204211R
ER  - 

@article{
author = "Radovanović, Anita M. and Mićić, Mileva and Gledić, Dušan S.",
year = "2002",
abstract = "The aim of this study was to investigate the localization and distribution of sympathetic positive structures in the female gonads and oviducts of 15 30 and 60-day-oldAO rats. Histofluorescence and immunohistochemistry methods were used to determine their distribution in the ovary and oviduct, as well as for identification of dopamine and serotonin within sympathetic positive structures. Total monoamine positive structures (fibers and cells) were mainly localized under the surface epithelium, between follicles, especially surrounding groups of primary follicles and between secondary interstitial cells. The distribution of dopamine (DA) immunolabelled fibers and cells was in accordance with the distribution of total monoaminergic structures detected using histofluorescence. However, the density of structures labeled with DA antibodies was lower compared to total catecholamine positive structures detected by the fluorescence method. Delicate serotonin (5-HT) positive fibers were present mainly under the surface epithelium and in interstitial tissue between the cortex and medulla. In the hilum and medulla 5-HT positive cells were found perivascularly, while a dense population of 5-HT positive cells was found in the oviduct and mesovarium in 60-day-old animals. No corpus luteum cells were labelled using either of the two methods. Our findings corroborate observations that the monoamine supply of the ovary is not exclusively via extrinsic innervation and that intragonadal sources have an important role in their synthesis. Furthermore, the intraovarian localization of these elements suggests that monoamines exert direct or indirect effects on ovarian function., Cilj našeg rada je bio ispitivanje lokalizacije i distribucije struktura pozitivnih na monoaminske neurotransmitere u jajnicima i jajovodima pacova. Za identifikaciju neurotransmitera u jajnicima i jajovodima 15, 30 i 60 dana starih AO pacova koristili smo histofluorescenciju (ukupni monoamini) i imunocitohemiju (dopamin i serotonin). Pozitivne monoaminergičke strukture prisutne su između folikula, naročito oko primarnih folikula, kao i između sekundarnih intersticijalnih ćelija. U mezoovarijumu i jajovodu 30 i 60 dana starih životinja zapažaju se fluorescentne ćelije i vlakna. Dopaminergične strukture su prisutne na istim mestima, ali njihova gustina je manja ako se uporede sa strukturama pozitivnim na ukupne monoamine. Retka serotonin-pozitivna vlakna zapažaju se uglavnom ispod klicinog epitela i u intersticijumu između kore i srži jajnika. Kod 30 i 60 dana starih ženki u hilusu i srži, pretežno oko krvnih sudova, nalaze se serotonin pozitivne ćelije, dok se u mezoovarijumu i jajovodu nalazi gusta populacija pozitivnih ćelija. Ni jednom od primenjenih metoda nije uočeno prisustvo monoamina u žutom telu. Naši rezultati potvrduju da neurotransmiteri u jajnicima i jajovodima ne potiču samo iz nervnih terminala, već da značajnu ulogu u njihovoj sintezi imaju intragonadalni izvori. Lokalizacija ovih elemenata pokazuje da monoaminski neurotransmiteri mogu ispoljavati direktan i!i indirektan uticaj na funkciju jajnika.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Localization of-monoamine positive structures in the ovary and oviduct of developing rats, Lokalizacija monoaminskih neurotransmitera u jajnicima i jajovodima pacova tokom polnog sazrevanja",
pages = "222-211",
number = "4",
volume = "52",
doi = "10.2298/AVB0204211R"
}

Radovanović, A. M., Mićić, M.,& Gledić, D. S.. (2002). Localization of-monoamine positive structures in the ovary and oviduct of developing rats. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 52(4), 211-222.
https://doi.org/10.2298/AVB0204211R

Radovanović AM, Mićić M, Gledić DS. Localization of-monoamine positive structures in the ovary and oviduct of developing rats. in Acta veterinaria - Beograd. 2002;52(4):211-222.
doi:10.2298/AVB0204211R .

Radovanović, Anita M., Mićić, Mileva, Gledić, Dušan S., "Localization of-monoamine positive structures in the ovary and oviduct of developing rats" in Acta veterinaria - Beograd, 52, no. 4 (2002):211-222,
https://doi.org/10.2298/AVB0204211R . .