TY  - JOUR
AU  - Petrović-Đergović, Danica M.
AU  - Živković, Irena
AU  - Rakin, Ana
AU  - Kosec, Duško
AU  - Dimitrijević, Ljiljana
AU  - Starčević, V.P.
AU  - Severs, W.B.
AU  - Mićić, Mileva
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/171
AB  - It is well known that somatostatin exerts a wide range of effects in the body, and acts as an antocrine or paracrine factor in the thymus. However, it has not been investigated yet whether somatostatin alters the thymus size and relation among the thymocyte subpopulations in the peripubertal rats. For this purpose, the peripubertal AO male rats were cannulated intracerebroventriculary and treated with repeated, low doses of somatostatin-14 (experimental group) or saline (control group). Twenty-four hours after the last treatment, we removed and prepared the thymuses for determination of thymocyte subpopulations by flow cytometry. After five days, animals were sacrificed and their thymuses taken for morphometrical analysis by stereological methods. We noticed that somatostatin-14 decreased volumes of thymus cortex and medulla, total number of thymocytes, number of thymocytes in the cortex and medulla and numerical density of thymocytes in deeper cortex. As a consequence of these changes, thymus size was also diminished. The phenotypic analysis of thymocyte subpopulations showed that somatostatin-14 decreased the percentage of CD4(+)CD8(+) cells with low level of TCRalphabeta expression, positively selected CD4(+)CD8(+)TCRalphabeta(high) cells and the most mature CD4(-)CD8(+)TCRalphabeta(high) cells, while the percentage of CD4(+)CD8(-)TCRalphabeta(high) thymocytes was slightly increased. Somatostatin-14 increased the relative proportion of the least mature CD4(-)CD8(-)TCRalphabeta(-/low), CD4(+)CD8(+)TCRalphabeta(-) cells and both of TCRalphabeta(-/low) single positive subpopulations. These results show that centrally applied somatostatin-14, induces hypotrophy of the thymus in peripubertal rats by changing the volumes and cellularities of the thymic compartments. Additionally, increased number of the least mature thymocytes and a deficiency of double positive cells indicate the involvement of somatostatin in the modulation of T cells maturation. (C) 2003 Elsevier Ltd. All rights reserved.
PB  - Churchill Livingstone, Edinburgh
T2  - Neuropeptides
T1  - Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats
EP  - 34
IS  - 1
SP  - 25
VL  - 38
DO  - 10.1016/j.npep.2003.11.003
ER  - 

@article{
author = "Petrović-Đergović, Danica M. and Živković, Irena and Rakin, Ana and Kosec, Duško and Dimitrijević, Ljiljana and Starčević, V.P. and Severs, W.B. and Mićić, Mileva",
year = "2004",
abstract = "It is well known that somatostatin exerts a wide range of effects in the body, and acts as an antocrine or paracrine factor in the thymus. However, it has not been investigated yet whether somatostatin alters the thymus size and relation among the thymocyte subpopulations in the peripubertal rats. For this purpose, the peripubertal AO male rats were cannulated intracerebroventriculary and treated with repeated, low doses of somatostatin-14 (experimental group) or saline (control group). Twenty-four hours after the last treatment, we removed and prepared the thymuses for determination of thymocyte subpopulations by flow cytometry. After five days, animals were sacrificed and their thymuses taken for morphometrical analysis by stereological methods. We noticed that somatostatin-14 decreased volumes of thymus cortex and medulla, total number of thymocytes, number of thymocytes in the cortex and medulla and numerical density of thymocytes in deeper cortex. As a consequence of these changes, thymus size was also diminished. The phenotypic analysis of thymocyte subpopulations showed that somatostatin-14 decreased the percentage of CD4(+)CD8(+) cells with low level of TCRalphabeta expression, positively selected CD4(+)CD8(+)TCRalphabeta(high) cells and the most mature CD4(-)CD8(+)TCRalphabeta(high) cells, while the percentage of CD4(+)CD8(-)TCRalphabeta(high) thymocytes was slightly increased. Somatostatin-14 increased the relative proportion of the least mature CD4(-)CD8(-)TCRalphabeta(-/low), CD4(+)CD8(+)TCRalphabeta(-) cells and both of TCRalphabeta(-/low) single positive subpopulations. These results show that centrally applied somatostatin-14, induces hypotrophy of the thymus in peripubertal rats by changing the volumes and cellularities of the thymic compartments. Additionally, increased number of the least mature thymocytes and a deficiency of double positive cells indicate the involvement of somatostatin in the modulation of T cells maturation. (C) 2003 Elsevier Ltd. All rights reserved.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Neuropeptides",
title = "Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats",
pages = "34-25",
number = "1",
volume = "38",
doi = "10.1016/j.npep.2003.11.003"
}

Petrović-Đergović, D. M., Živković, I., Rakin, A., Kosec, D., Dimitrijević, L., Starčević, V.P., Severs, W.B.,& Mićić, M.. (2004). Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats. in Neuropeptides
Churchill Livingstone, Edinburgh., 38(1), 25-34.
https://doi.org/10.1016/j.npep.2003.11.003

Petrović-Đergović DM, Živković I, Rakin A, Kosec D, Dimitrijević L, Starčević V, Severs W, Mićić M. Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats. in Neuropeptides. 2004;38(1):25-34.
doi:10.1016/j.npep.2003.11.003 .

Petrović-Đergović, Danica M., Živković, Irena, Rakin, Ana, Kosec, Duško, Dimitrijević, Ljiljana, Starčević, V.P., Severs, W.B., Mićić, Mileva, "Somatostatin-14 alters the thymus size and relation among the thymocyte subpopulations in peripubertal rats" in Neuropeptides, 38, no. 1 (2004):25-34,
https://doi.org/10.1016/j.npep.2003.11.003 . .