TY  - JOUR
AU  - Petrović-Đergović, Danica M.
AU  - Rakin, Ana
AU  - Dimitrijević, Ljiljana
AU  - Ristovski, Jasmina S.
AU  - Kuštrimović, Nataša
AU  - Mićić, Mileva
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/241
AB  - The role of somatostatin on inhibition of both normal and tumor cell cycle, secretion of endocrine and exocrine cells, as well as induction apoptosis is well documented. However, its effect on T cell development and thymic structure is not fully clarified. In order to investigate the influence of somatostatin in vivo on the thymus structure and T cell development, the young adult Albino Oxford male rats were intracerebroventriculary treated with somatostatin-14. We examined the thymus compartments and its cellularity, through assessment of morphometric parameters by stereological method, and the relation between thymocytes subpopulations, over expression of CD4, CD8 and T-cell receptor (TCR) alpha beta by flow cytometry. Additionally, we also determined the body and thymus weight of the rats, during the first three months of life, to define the time of SRIH-14 application. A decrease of relative thymus weight from the fourth weeks of postnatal life, and an unchanged relative thymus weight obtained in treated group indicates that SRIH-14 in young adult rats inhibits growth of whole organism, not only thymus. The changes in the absolute number and numerical density of cortical thymocytes indicate that SRIH-14 alters the true lymphoid tissue. SRIH-14 changes relation between thymocyte subsets, increase number of CD4(-)CD8(-)TCR alpha beta(-) and CD4(-)CD8(+)TCR alpha beta(hi) phi thymocyte subsets as well as the CD4(-)CD8(-)TCR alpha beta(low/hi) thymocytes, while decrease number of CD4+CD8+ TCR alpha beta(low/hi) thymocyte subsets. These results indicate that somatostatin-14 is Dot involved in the control of the physiologic involution of the thymus, although induces thymic weight loss through the reduction of true lymphoid tissue. In addition, changes in frequency of thymocyte subpopulations, especially immature cells, indicate that SRIH-14 modulates thymocytes development and maturation. (C) 2007 Elsevier Ltd. All rights reserved.
PB  - Churchill Livingstone, Edinburgh
T2  - Neuropeptides
T1  - Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14
EP  - 493
IS  - 6
SP  - 485
VL  - 41
DO  - 10.1016/j.npep.2007.06.003
ER  - 

@article{
author = "Petrović-Đergović, Danica M. and Rakin, Ana and Dimitrijević, Ljiljana and Ristovski, Jasmina S. and Kuštrimović, Nataša and Mićić, Mileva",
year = "2007",
abstract = "The role of somatostatin on inhibition of both normal and tumor cell cycle, secretion of endocrine and exocrine cells, as well as induction apoptosis is well documented. However, its effect on T cell development and thymic structure is not fully clarified. In order to investigate the influence of somatostatin in vivo on the thymus structure and T cell development, the young adult Albino Oxford male rats were intracerebroventriculary treated with somatostatin-14. We examined the thymus compartments and its cellularity, through assessment of morphometric parameters by stereological method, and the relation between thymocytes subpopulations, over expression of CD4, CD8 and T-cell receptor (TCR) alpha beta by flow cytometry. Additionally, we also determined the body and thymus weight of the rats, during the first three months of life, to define the time of SRIH-14 application. A decrease of relative thymus weight from the fourth weeks of postnatal life, and an unchanged relative thymus weight obtained in treated group indicates that SRIH-14 in young adult rats inhibits growth of whole organism, not only thymus. The changes in the absolute number and numerical density of cortical thymocytes indicate that SRIH-14 alters the true lymphoid tissue. SRIH-14 changes relation between thymocyte subsets, increase number of CD4(-)CD8(-)TCR alpha beta(-) and CD4(-)CD8(+)TCR alpha beta(hi) phi thymocyte subsets as well as the CD4(-)CD8(-)TCR alpha beta(low/hi) thymocytes, while decrease number of CD4+CD8+ TCR alpha beta(low/hi) thymocyte subsets. These results indicate that somatostatin-14 is Dot involved in the control of the physiologic involution of the thymus, although induces thymic weight loss through the reduction of true lymphoid tissue. In addition, changes in frequency of thymocyte subpopulations, especially immature cells, indicate that SRIH-14 modulates thymocytes development and maturation. (C) 2007 Elsevier Ltd. All rights reserved.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Neuropeptides",
title = "Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14",
pages = "493-485",
number = "6",
volume = "41",
doi = "10.1016/j.npep.2007.06.003"
}

Petrović-Đergović, D. M., Rakin, A., Dimitrijević, L., Ristovski, J. S., Kuštrimović, N.,& Mićić, M.. (2007). Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14. in Neuropeptides
Churchill Livingstone, Edinburgh., 41(6), 485-493.
https://doi.org/10.1016/j.npep.2007.06.003

Petrović-Đergović DM, Rakin A, Dimitrijević L, Ristovski JS, Kuštrimović N, Mićić M. Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14. in Neuropeptides. 2007;41(6):485-493.
doi:10.1016/j.npep.2007.06.003 .

Petrović-Đergović, Danica M., Rakin, Ana, Dimitrijević, Ljiljana, Ristovski, Jasmina S., Kuštrimović, Nataša, Mićić, Mileva, "Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by Somatostatin-14" in Neuropeptides, 41, no. 6 (2007):485-493,
https://doi.org/10.1016/j.npep.2007.06.003 . .

TY  - JOUR
AU  - Petrović-Đergović, Danica M.
AU  - Rakin, Ana
AU  - Kuštrimović, Nataša
AU  - Ristovski, Jasmina S.
AU  - Dimitrijević, Ljiljana
AU  - Mićić, Mileva
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/233
AB  - It is well known that somatostatin modulates thymic functions, such as binding to receptors. In order to elucidate the influence of somatostatin on the thymus architecture and the T cells maturation, young adult male rats were treated with somatostatin-28. The results showed that somatostatin-28 decreased thymus weight and cellularity, probably due to alterations in the thymic morphometric parameters. Our results also demonstrated that SRIH treatment reduces number of cells with undetectable alpha beta TCR and cells with low expression of alpha beta TCR, while the number of TCR alpha beta(hi) cells remains approximately the same as the values obtained from the control rats. Besides, in the least mature thymocytes (DNTCR TCR alpha beta(-)) and among the most mature the SPCD4 TCR alpha beta(hi) subset remained unaltered, while SPCD8 TCR alpha beta(hi) decreased. At last, it should be noted that SRIH treatment increases DN thymocytes subsets expressing TCR alpha beta(low/hi) (TCR alpha beta(+)). These results suggest that somatostatin-28 induces reshaping of T cells maturation and, at least partly, contributes to thymic weight loss, through the modulation of the complex neuroendocrine-immune network. (C) 2007 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Regulatory Peptides
T1  - Somatostatin modulates T cells development in adult rat thymus
EP  - 110
IS  - 3
SP  - 101
VL  - 142
DO  - 10.1016/j.regpep.2007.02.003
ER  - 

@article{
author = "Petrović-Đergović, Danica M. and Rakin, Ana and Kuštrimović, Nataša and Ristovski, Jasmina S. and Dimitrijević, Ljiljana and Mićić, Mileva",
year = "2007",
abstract = "It is well known that somatostatin modulates thymic functions, such as binding to receptors. In order to elucidate the influence of somatostatin on the thymus architecture and the T cells maturation, young adult male rats were treated with somatostatin-28. The results showed that somatostatin-28 decreased thymus weight and cellularity, probably due to alterations in the thymic morphometric parameters. Our results also demonstrated that SRIH treatment reduces number of cells with undetectable alpha beta TCR and cells with low expression of alpha beta TCR, while the number of TCR alpha beta(hi) cells remains approximately the same as the values obtained from the control rats. Besides, in the least mature thymocytes (DNTCR TCR alpha beta(-)) and among the most mature the SPCD4 TCR alpha beta(hi) subset remained unaltered, while SPCD8 TCR alpha beta(hi) decreased. At last, it should be noted that SRIH treatment increases DN thymocytes subsets expressing TCR alpha beta(low/hi) (TCR alpha beta(+)). These results suggest that somatostatin-28 induces reshaping of T cells maturation and, at least partly, contributes to thymic weight loss, through the modulation of the complex neuroendocrine-immune network. (C) 2007 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Somatostatin modulates T cells development in adult rat thymus",
pages = "110-101",
number = "3",
volume = "142",
doi = "10.1016/j.regpep.2007.02.003"
}

Petrović-Đergović, D. M., Rakin, A., Kuštrimović, N., Ristovski, J. S., Dimitrijević, L.,& Mićić, M.. (2007). Somatostatin modulates T cells development in adult rat thymus. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 142(3), 101-110.
https://doi.org/10.1016/j.regpep.2007.02.003

Petrović-Đergović DM, Rakin A, Kuštrimović N, Ristovski JS, Dimitrijević L, Mićić M. Somatostatin modulates T cells development in adult rat thymus. in Regulatory Peptides. 2007;142(3):101-110.
doi:10.1016/j.regpep.2007.02.003 .

Petrović-Đergović, Danica M., Rakin, Ana, Kuštrimović, Nataša, Ristovski, Jasmina S., Dimitrijević, Ljiljana, Mićić, Mileva, "Somatostatin modulates T cells development in adult rat thymus" in Regulatory Peptides, 142, no. 3 (2007):101-110,
https://doi.org/10.1016/j.regpep.2007.02.003 . .