TY  - JOUR
AU  - Tasić, S.
AU  - Kojić, Milan
AU  - Stanković, Slaviša
AU  - Obradović, D.
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/569
UR  - http://intor.torlakinstitut.com/handle/123456789/752
AB  - The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia
EP  - 883
IS  - 3
SP  - 877
VL  - 64
DO  - 10.2298/ABS1203877T
ER  - 

@article{
author = "Tasić, S. and Kojić, Milan and Stanković, Slaviša and Obradović, D.",
year = "2012",
abstract = "The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia",
pages = "883-877",
number = "3",
volume = "64",
doi = "10.2298/ABS1203877T"
}

Tasić, S., Kojić, M., Stanković, S.,& Obradović, D.. (2012). Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(3), 877-883.
https://doi.org/10.2298/ABS1203877T

Tasić S, Kojić M, Stanković S, Obradović D. Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia. in Archives of Biological Sciences. 2012;64(3):877-883.
doi:10.2298/ABS1203877T .

Tasić, S., Kojić, Milan, Stanković, Slaviša, Obradović, D., "Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia" in Archives of Biological Sciences, 64, no. 3 (2012):877-883,
https://doi.org/10.2298/ABS1203877T . .