The effects of Lactobacillus rhamnosus lb64 on translocation of enteric bacteria and splenic no production during colitis
Само за регистроване кориснике
2017
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
INTRODUCTION: It has been described that loss of intestinal barrier integrity and consequent
translocation of gut microbiota toward extraintestinal sites contribute to colitis development. As
Lactobacillus rhamnosus LB64 (Liobif®, produced by Institute ,,Torlak", Belgrade) is widely used as
an antidiarrheal drug, we sought to determine if it has potential to affect translocation of gut
microbiota during colitis.
GOAL: We studied the influence of oral administration of LB64 on a) translocation of enteric bacteria
and b) its own potential to translocate from gut to lymphoid organs under physiological conditions
and during colitis. Besides, we have determined splenic nitric oxide (NO) production.
METHODS: Rats of DA strain were given a solution containing LB64 (3 x 10¢/ml/kg) during early
postnatal (3.-30. day of life, LB/H20), young adult period (31.-70. day of life, H20/LB) or were given
LB (LB/LB) or tap water (H20/H20) throughout (3.-70. day of life). Colitis was induced by ...intrarectal
administration of 40mg/kg TNBS in 50% ethanol, Seven days later, spleen and mesenteric lymph
nodes (MLN) were excized, macerated and plated on Mac Concey agar and MRS (37°C) for 24h and
48h, respectively. NO production was determined by Griess reaction in supernatants of homogenized
spleen tissue.
RESULT: No presence of LB64 was discovered in MLN or spleens of either healthy or colitic rats.
However, both E.coli and Enterococcus spp. were detected in MLN and spleens of rats with colitis but
not in lymphoid organs of healthy rats. Treatments with LB didn't affect colitis-induced enteric
bacteria traslocation, but prevented colitis-induced increase in splenic NO production.
CONCLUSION: Although LB did not affect bacterial translocation during colitis, it exerted antiinflammatory
effect on a systemic level without leaving the gut. Our results suggest
immunomodulatory activity of LB64 (Supported by Ministry of Education, Science and Technological
Development Republic of Serbia, Grant No 175050.).
Кључне речи:
Lactobacillus LB 64 / colitis / splenic NO productionИзвор:
Xl Kongres mikrobiologa Srbije Mikromed sa međunarodnim učešćem 11-13. maj 2017., 2017, 168-168Издавач:
- Udruženje mikrobiologa Srbije
Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
Институција/група
TorlakTY - CONF AU - Stanojević, Stanislava AU - Ćuruvija, Ivana AU - Cvetković, Vesna AU - Kovačević-Jovanović, Vesna PY - 2017 UR - http://intor.torlakinstitut.com/handle/123456789/895 AB - INTRODUCTION: It has been described that loss of intestinal barrier integrity and consequent translocation of gut microbiota toward extraintestinal sites contribute to colitis development. As Lactobacillus rhamnosus LB64 (Liobif®, produced by Institute ,,Torlak", Belgrade) is widely used as an antidiarrheal drug, we sought to determine if it has potential to affect translocation of gut microbiota during colitis. GOAL: We studied the influence of oral administration of LB64 on a) translocation of enteric bacteria and b) its own potential to translocate from gut to lymphoid organs under physiological conditions and during colitis. Besides, we have determined splenic nitric oxide (NO) production. METHODS: Rats of DA strain were given a solution containing LB64 (3 x 10¢/ml/kg) during early postnatal (3.-30. day of life, LB/H20), young adult period (31.-70. day of life, H20/LB) or were given LB (LB/LB) or tap water (H20/H20) throughout (3.-70. day of life). Colitis was induced by intrarectal administration of 40mg/kg TNBS in 50% ethanol, Seven days later, spleen and mesenteric lymph nodes (MLN) were excized, macerated and plated on Mac Concey agar and MRS (37°C) for 24h and 48h, respectively. NO production was determined by Griess reaction in supernatants of homogenized spleen tissue. RESULT: No presence of LB64 was discovered in MLN or spleens of either healthy or colitic rats. However, both E.coli and Enterococcus spp. were detected in MLN and spleens of rats with colitis but not in lymphoid organs of healthy rats. Treatments with LB didn't affect colitis-induced enteric bacteria traslocation, but prevented colitis-induced increase in splenic NO production. CONCLUSION: Although LB did not affect bacterial translocation during colitis, it exerted antiinflammatory effect on a systemic level without leaving the gut. Our results suggest immunomodulatory activity of LB64 (Supported by Ministry of Education, Science and Technological Development Republic of Serbia, Grant No 175050.). PB - Udruženje mikrobiologa Srbije C3 - Xl Kongres mikrobiologa Srbije Mikromed sa međunarodnim učešćem 11-13. maj 2017. T1 - The effects of Lactobacillus rhamnosus lb64 on translocation of enteric bacteria and splenic no production during colitis EP - 168 SP - 168 UR - https://hdl.handle.net/21.15107/rcub_intor_895 ER -
@conference{ author = "Stanojević, Stanislava and Ćuruvija, Ivana and Cvetković, Vesna and Kovačević-Jovanović, Vesna", year = "2017", abstract = "INTRODUCTION: It has been described that loss of intestinal barrier integrity and consequent translocation of gut microbiota toward extraintestinal sites contribute to colitis development. As Lactobacillus rhamnosus LB64 (Liobif®, produced by Institute ,,Torlak", Belgrade) is widely used as an antidiarrheal drug, we sought to determine if it has potential to affect translocation of gut microbiota during colitis. GOAL: We studied the influence of oral administration of LB64 on a) translocation of enteric bacteria and b) its own potential to translocate from gut to lymphoid organs under physiological conditions and during colitis. Besides, we have determined splenic nitric oxide (NO) production. METHODS: Rats of DA strain were given a solution containing LB64 (3 x 10¢/ml/kg) during early postnatal (3.-30. day of life, LB/H20), young adult period (31.-70. day of life, H20/LB) or were given LB (LB/LB) or tap water (H20/H20) throughout (3.-70. day of life). Colitis was induced by intrarectal administration of 40mg/kg TNBS in 50% ethanol, Seven days later, spleen and mesenteric lymph nodes (MLN) were excized, macerated and plated on Mac Concey agar and MRS (37°C) for 24h and 48h, respectively. NO production was determined by Griess reaction in supernatants of homogenized spleen tissue. RESULT: No presence of LB64 was discovered in MLN or spleens of either healthy or colitic rats. However, both E.coli and Enterococcus spp. were detected in MLN and spleens of rats with colitis but not in lymphoid organs of healthy rats. Treatments with LB didn't affect colitis-induced enteric bacteria traslocation, but prevented colitis-induced increase in splenic NO production. CONCLUSION: Although LB did not affect bacterial translocation during colitis, it exerted antiinflammatory effect on a systemic level without leaving the gut. Our results suggest immunomodulatory activity of LB64 (Supported by Ministry of Education, Science and Technological Development Republic of Serbia, Grant No 175050.).", publisher = "Udruženje mikrobiologa Srbije", journal = "Xl Kongres mikrobiologa Srbije Mikromed sa međunarodnim učešćem 11-13. maj 2017.", title = "The effects of Lactobacillus rhamnosus lb64 on translocation of enteric bacteria and splenic no production during colitis", pages = "168-168", url = "https://hdl.handle.net/21.15107/rcub_intor_895" }
Stanojević, S., Ćuruvija, I., Cvetković, V.,& Kovačević-Jovanović, V.. (2017). The effects of Lactobacillus rhamnosus lb64 on translocation of enteric bacteria and splenic no production during colitis. in Xl Kongres mikrobiologa Srbije Mikromed sa međunarodnim učešćem 11-13. maj 2017. Udruženje mikrobiologa Srbije., 168-168. https://hdl.handle.net/21.15107/rcub_intor_895
Stanojević S, Ćuruvija I, Cvetković V, Kovačević-Jovanović V. The effects of Lactobacillus rhamnosus lb64 on translocation of enteric bacteria and splenic no production during colitis. in Xl Kongres mikrobiologa Srbije Mikromed sa međunarodnim učešćem 11-13. maj 2017.. 2017;:168-168. https://hdl.handle.net/21.15107/rcub_intor_895 .
Stanojević, Stanislava, Ćuruvija, Ivana, Cvetković, Vesna, Kovačević-Jovanović, Vesna, "The effects of Lactobacillus rhamnosus lb64 on translocation of enteric bacteria and splenic no production during colitis" in Xl Kongres mikrobiologa Srbije Mikromed sa međunarodnim učešćem 11-13. maj 2017. (2017):168-168, https://hdl.handle.net/21.15107/rcub_intor_895 .