Age-associated shift in rat dendritic cell T-helper polarizing capacity
Autori
Bufan, BiljanaStojić-Vukanić, Zorica
Arsenović-Ranin, Nevena
Kosec, Duško
Pilipović, Ivan
Perišić, Milica
Đikić, Jasmina
Leposavić, Gordana
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Almost all cellular components of innate and adaptive immunity undergo age-related remodeling. The findings on age-related
changes in human and mouse dendritic cells (DCs) are conflicting, whereas there is no data on the influence of aging on rat DCs. In
attempt to fill this gap, freshly isolated splenic conventional OX62+ DCs from 3- (young) and 26-month-old (aged) Albino Oxford rats
were examined for subset composition, cell surface expression of activation markers (CD80, CD86 and CD40 and MHC II molecules)
and endocytic capacity using flow cytometric analysis (FCA). In addition, splenic OX62+ DCs isolated from rats of both ages were
cultured in the presence or in the absence of LPS. These cells were examined for the activation marker and TNF-α, IL-6, IL-12, IL-23,
TGF-β1, IL-10 expression using FCA, and RT-PCR and ELISA, respectively. Moreover, the allostimulatory capacity of OX62+ DCs and
allogeneic CD4+ T cell cytokine (IFN-γ, IL-4 and IL-17) production in MLR was quantifie...d using FCA and ELISA, respectively. It was
found that aging: i) in OX62+ DCs population leads to a shift in CD4+:CD4- cell ratio towards CD4- cells and ii) influences OX62+
DCs maturation capacity (judging by activation marker expression and efficiency of endocytosis) by affecting action of intrinsic (TNF-
α and IL-10) and extrinsic regulatory factor expression. Furthermore, in LPS-matured OX62+ DCs from aged rats TNF-α, IL-12, IL-23
and IL-6 expression was increased, while IL-10 expression was diminished. Moreover, in MLR, OX62+ DCs from aged rats exhibited
enhanced Th1/Th17 driving force and diminished allostimulatory capacity.
Ključne reči:
rat / splenic conventional dendritic cells / Aging / Cytokine expression / Th polarizationIzvor:
Frontiers in Immunology, 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013Izdavač:
- Frontiers Media
Finansiranje / projekti:
- Plastičnost imunskog sistema tokom starenja: imunomodulatorni potencijal estrogena (RS-MESTD-Basic Research (BR or ON)-175050)
Institucija/grupa
TorlakTY - CONF AU - Bufan, Biljana AU - Stojić-Vukanić, Zorica AU - Arsenović-Ranin, Nevena AU - Kosec, Duško AU - Pilipović, Ivan AU - Perišić, Milica AU - Đikić, Jasmina AU - Leposavić, Gordana PY - 2013 UR - http://intor.torlakinstitut.com/handle/123456789/849 AB - Almost all cellular components of innate and adaptive immunity undergo age-related remodeling. The findings on age-related changes in human and mouse dendritic cells (DCs) are conflicting, whereas there is no data on the influence of aging on rat DCs. In attempt to fill this gap, freshly isolated splenic conventional OX62+ DCs from 3- (young) and 26-month-old (aged) Albino Oxford rats were examined for subset composition, cell surface expression of activation markers (CD80, CD86 and CD40 and MHC II molecules) and endocytic capacity using flow cytometric analysis (FCA). In addition, splenic OX62+ DCs isolated from rats of both ages were cultured in the presence or in the absence of LPS. These cells were examined for the activation marker and TNF-α, IL-6, IL-12, IL-23, TGF-β1, IL-10 expression using FCA, and RT-PCR and ELISA, respectively. Moreover, the allostimulatory capacity of OX62+ DCs and allogeneic CD4+ T cell cytokine (IFN-γ, IL-4 and IL-17) production in MLR was quantified using FCA and ELISA, respectively. It was found that aging: i) in OX62+ DCs population leads to a shift in CD4+:CD4- cell ratio towards CD4- cells and ii) influences OX62+ DCs maturation capacity (judging by activation marker expression and efficiency of endocytosis) by affecting action of intrinsic (TNF- α and IL-10) and extrinsic regulatory factor expression. Furthermore, in LPS-matured OX62+ DCs from aged rats TNF-α, IL-12, IL-23 and IL-6 expression was increased, while IL-10 expression was diminished. Moreover, in MLR, OX62+ DCs from aged rats exhibited enhanced Th1/Th17 driving force and diminished allostimulatory capacity. PB - Frontiers Media C3 - Frontiers in Immunology, 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug T1 - Age-associated shift in rat dendritic cell T-helper polarizing capacity DO - 10.3389/conf.fimmu.2013.02.00138 ER -
@conference{ author = "Bufan, Biljana and Stojić-Vukanić, Zorica and Arsenović-Ranin, Nevena and Kosec, Duško and Pilipović, Ivan and Perišić, Milica and Đikić, Jasmina and Leposavić, Gordana", year = "2013", abstract = "Almost all cellular components of innate and adaptive immunity undergo age-related remodeling. The findings on age-related changes in human and mouse dendritic cells (DCs) are conflicting, whereas there is no data on the influence of aging on rat DCs. In attempt to fill this gap, freshly isolated splenic conventional OX62+ DCs from 3- (young) and 26-month-old (aged) Albino Oxford rats were examined for subset composition, cell surface expression of activation markers (CD80, CD86 and CD40 and MHC II molecules) and endocytic capacity using flow cytometric analysis (FCA). In addition, splenic OX62+ DCs isolated from rats of both ages were cultured in the presence or in the absence of LPS. These cells were examined for the activation marker and TNF-α, IL-6, IL-12, IL-23, TGF-β1, IL-10 expression using FCA, and RT-PCR and ELISA, respectively. Moreover, the allostimulatory capacity of OX62+ DCs and allogeneic CD4+ T cell cytokine (IFN-γ, IL-4 and IL-17) production in MLR was quantified using FCA and ELISA, respectively. It was found that aging: i) in OX62+ DCs population leads to a shift in CD4+:CD4- cell ratio towards CD4- cells and ii) influences OX62+ DCs maturation capacity (judging by activation marker expression and efficiency of endocytosis) by affecting action of intrinsic (TNF- α and IL-10) and extrinsic regulatory factor expression. Furthermore, in LPS-matured OX62+ DCs from aged rats TNF-α, IL-12, IL-23 and IL-6 expression was increased, while IL-10 expression was diminished. Moreover, in MLR, OX62+ DCs from aged rats exhibited enhanced Th1/Th17 driving force and diminished allostimulatory capacity.", publisher = "Frontiers Media", journal = "Frontiers in Immunology, 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug", title = "Age-associated shift in rat dendritic cell T-helper polarizing capacity", doi = "10.3389/conf.fimmu.2013.02.00138" }
Bufan, B., Stojić-Vukanić, Z., Arsenović-Ranin, N., Kosec, D., Pilipović, I., Perišić, M., Đikić, J.,& Leposavić, G.. (2013). Age-associated shift in rat dendritic cell T-helper polarizing capacity. in Frontiers in Immunology, 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug Frontiers Media.. https://doi.org/10.3389/conf.fimmu.2013.02.00138
Bufan B, Stojić-Vukanić Z, Arsenović-Ranin N, Kosec D, Pilipović I, Perišić M, Đikić J, Leposavić G. Age-associated shift in rat dendritic cell T-helper polarizing capacity. in Frontiers in Immunology, 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug. 2013;. doi:10.3389/conf.fimmu.2013.02.00138 .
Bufan, Biljana, Stojić-Vukanić, Zorica, Arsenović-Ranin, Nevena, Kosec, Duško, Pilipović, Ivan, Perišić, Milica, Đikić, Jasmina, Leposavić, Gordana, "Age-associated shift in rat dendritic cell T-helper polarizing capacity" in Frontiers in Immunology, 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug (2013), https://doi.org/10.3389/conf.fimmu.2013.02.00138 . .