The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis
Samo za registrovane korisnike
2013
Autori
Filipić, BrankicaGolić, Nataša
Jovčić, Branko
Tolinački, Maja
Bay, Denice C.
Turner, Raymond J.
Antić-Stanković, Jelena
Kojić, Milan
Topisirović, Ljubiša
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this s...tudy, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism.
Ključne reči:
Sulfur metabolism / Multidrug resistance / Major facilitator superfamily / Lactococcus lactis / CmbT transporterIzvor:
Research in Microbiology, 2013, 164, 1, 46-54Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
- Natural Sciences and Engineering Research Council of Canada
DOI: 10.1016/j.resmic.2012.09.003
ISSN: 0923-2508
PubMed: 22985829
WoS: 000313542400008
Scopus: 2-s2.0-84871720792
URI
https://imagine.imgge.bg.ac.rs/handle/123456789/618http://intor.torlakinstitut.com/handle/123456789/831
Institucija/grupa
TorlakTY - JOUR AU - Filipić, Brankica AU - Golić, Nataša AU - Jovčić, Branko AU - Tolinački, Maja AU - Bay, Denice C. AU - Turner, Raymond J. AU - Antić-Stanković, Jelena AU - Kojić, Milan AU - Topisirović, Ljubiša PY - 2013 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/618 UR - http://intor.torlakinstitut.com/handle/123456789/831 AB - Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this study, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism. PB - Elsevier, Amsterdam T2 - Research in Microbiology T1 - The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis EP - 54 IS - 1 SP - 46 VL - 164 DO - 10.1016/j.resmic.2012.09.003 ER -
@article{ author = "Filipić, Brankica and Golić, Nataša and Jovčić, Branko and Tolinački, Maja and Bay, Denice C. and Turner, Raymond J. and Antić-Stanković, Jelena and Kojić, Milan and Topisirović, Ljubiša", year = "2013", abstract = "Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this study, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism.", publisher = "Elsevier, Amsterdam", journal = "Research in Microbiology", title = "The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis", pages = "54-46", number = "1", volume = "164", doi = "10.1016/j.resmic.2012.09.003" }
Filipić, B., Golić, N., Jovčić, B., Tolinački, M., Bay, D. C., Turner, R. J., Antić-Stanković, J., Kojić, M.,& Topisirović, L.. (2013). The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. in Research in Microbiology Elsevier, Amsterdam., 164(1), 46-54. https://doi.org/10.1016/j.resmic.2012.09.003
Filipić B, Golić N, Jovčić B, Tolinački M, Bay DC, Turner RJ, Antić-Stanković J, Kojić M, Topisirović L. The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. in Research in Microbiology. 2013;164(1):46-54. doi:10.1016/j.resmic.2012.09.003 .
Filipić, Brankica, Golić, Nataša, Jovčić, Branko, Tolinački, Maja, Bay, Denice C., Turner, Raymond J., Antić-Stanković, Jelena, Kojić, Milan, Topisirović, Ljubiša, "The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis" in Research in Microbiology, 164, no. 1 (2013):46-54, https://doi.org/10.1016/j.resmic.2012.09.003 . .