Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50
Само за регистроване кориснике
2005
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 mu g(.)mL(-1)) and sublethal temperature (40 degrees C) resulted in a very low yield (0.17%) of Prt(-), Bac(-), Bac(s) derivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combination with restriction enzyme analysis and DNA-DNA hybridization revealed that S50 contains 2 additional large plasmids: pS140 (140 kb) and pS80 (80 kb). Conjugation experiments using strain S50 as a donor and various lactococcal recipients resulted in Prt(+), Bac(+), Bac(r) transconjugants. Analysis of these transconjugants strongly indicated that plasmid pS140 harbours the prt ...and bac genes encoding proteinase and bacteriocin production, and immunity to bacteriocin, since each Prt(+), Bac(+), Bac(r) tranconjugant contained pS140. Accordingly, none of the Prt-, Bac-, Bacs transconjugants contained this plasmid. pS140 was a self-transmissible conjugative plasmid regardless of the host lactococcal recipient used in the test. Frequency of conjugation of plasmid pS140 did not depend on either the donor or recipient strain.
Кључне речи:
proteinase / plasmids / Lactococcus / conjugation / bacteriocinИзвор:
Canadian Journal of Microbiology, 2005, 51, 4, 305-314Издавач:
- Canadian Science Publishing, Nrc Research Press, Ottawa
DOI: 10.1139/W05-009
ISSN: 0008-4166
PubMed: 15980892
WoS: 000230893600004
Scopus: 2-s2.0-23844517059
Институција/група
TorlakTY - JOUR AU - Kojić, Milan AU - Strahinić, Ivana AU - Topisirović, Ljubiša PY - 2005 UR - http://intor.torlakinstitut.com/handle/123456789/809 AB - Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 mu g(.)mL(-1)) and sublethal temperature (40 degrees C) resulted in a very low yield (0.17%) of Prt(-), Bac(-), Bac(s) derivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combination with restriction enzyme analysis and DNA-DNA hybridization revealed that S50 contains 2 additional large plasmids: pS140 (140 kb) and pS80 (80 kb). Conjugation experiments using strain S50 as a donor and various lactococcal recipients resulted in Prt(+), Bac(+), Bac(r) transconjugants. Analysis of these transconjugants strongly indicated that plasmid pS140 harbours the prt and bac genes encoding proteinase and bacteriocin production, and immunity to bacteriocin, since each Prt(+), Bac(+), Bac(r) tranconjugant contained pS140. Accordingly, none of the Prt-, Bac-, Bacs transconjugants contained this plasmid. pS140 was a self-transmissible conjugative plasmid regardless of the host lactococcal recipient used in the test. Frequency of conjugation of plasmid pS140 did not depend on either the donor or recipient strain. PB - Canadian Science Publishing, Nrc Research Press, Ottawa T2 - Canadian Journal of Microbiology T1 - Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50 EP - 314 IS - 4 SP - 305 VL - 51 DO - 10.1139/W05-009 ER -
@article{ author = "Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša", year = "2005", abstract = "Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 mu g(.)mL(-1)) and sublethal temperature (40 degrees C) resulted in a very low yield (0.17%) of Prt(-), Bac(-), Bac(s) derivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combination with restriction enzyme analysis and DNA-DNA hybridization revealed that S50 contains 2 additional large plasmids: pS140 (140 kb) and pS80 (80 kb). Conjugation experiments using strain S50 as a donor and various lactococcal recipients resulted in Prt(+), Bac(+), Bac(r) transconjugants. Analysis of these transconjugants strongly indicated that plasmid pS140 harbours the prt and bac genes encoding proteinase and bacteriocin production, and immunity to bacteriocin, since each Prt(+), Bac(+), Bac(r) tranconjugant contained pS140. Accordingly, none of the Prt-, Bac-, Bacs transconjugants contained this plasmid. pS140 was a self-transmissible conjugative plasmid regardless of the host lactococcal recipient used in the test. Frequency of conjugation of plasmid pS140 did not depend on either the donor or recipient strain.", publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa", journal = "Canadian Journal of Microbiology", title = "Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50", pages = "314-305", number = "4", volume = "51", doi = "10.1139/W05-009" }
Kojić, M., Strahinić, I.,& Topisirović, L.. (2005). Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology Canadian Science Publishing, Nrc Research Press, Ottawa., 51(4), 305-314. https://doi.org/10.1139/W05-009
Kojić M, Strahinić I, Topisirović L. Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology. 2005;51(4):305-314. doi:10.1139/W05-009 .
Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50" in Canadian Journal of Microbiology, 51, no. 4 (2005):305-314, https://doi.org/10.1139/W05-009 . .