Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50
Abstract
Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 mu g(.)mL(-1)) and sublethal temperature (40 degrees C) resulted in a very low yield (0.17%) of Prt(-), Bac(-), Bac(s) derivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combination with restriction enzyme analysis and DNA-DNA hybridization revealed that S50 contains 2 additional large plasmids: pS140 (140 kb) and pS80 (80 kb). Conjugation experiments using strain S50 as a donor and various lactococcal recipients resulted in Prt(+), Bac(+), Bac(r) transconjugants. Analysis of these transconjugants strongly indicated that plasmid pS140 harbours the prt ...and bac genes encoding proteinase and bacteriocin production, and immunity to bacteriocin, since each Prt(+), Bac(+), Bac(r) tranconjugant contained pS140. Accordingly, none of the Prt-, Bac-, Bacs transconjugants contained this plasmid. pS140 was a self-transmissible conjugative plasmid regardless of the host lactococcal recipient used in the test. Frequency of conjugation of plasmid pS140 did not depend on either the donor or recipient strain.
Keywords:
proteinase / plasmids / Lactococcus / conjugation / bacteriocinSource:
Canadian Journal of Microbiology, 2005, 51, 4, 305-314Publisher:
- Canadian Science Publishing, Nrc Research Press, Ottawa
DOI: 10.1139/W05-009
ISSN: 0008-4166
PubMed: 15980892
WoS: 000230893600004
Scopus: 2-s2.0-23844517059
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Institution/Community
TorlakTY - JOUR AU - Kojić, Milan AU - Strahinić, Ivana AU - Topisirović, Ljubiša PY - 2005 UR - http://intor.torlakinstitut.com/handle/123456789/809 AB - Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 mu g(.)mL(-1)) and sublethal temperature (40 degrees C) resulted in a very low yield (0.17%) of Prt(-), Bac(-), Bac(s) derivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combination with restriction enzyme analysis and DNA-DNA hybridization revealed that S50 contains 2 additional large plasmids: pS140 (140 kb) and pS80 (80 kb). Conjugation experiments using strain S50 as a donor and various lactococcal recipients resulted in Prt(+), Bac(+), Bac(r) transconjugants. Analysis of these transconjugants strongly indicated that plasmid pS140 harbours the prt and bac genes encoding proteinase and bacteriocin production, and immunity to bacteriocin, since each Prt(+), Bac(+), Bac(r) tranconjugant contained pS140. Accordingly, none of the Prt-, Bac-, Bacs transconjugants contained this plasmid. pS140 was a self-transmissible conjugative plasmid regardless of the host lactococcal recipient used in the test. Frequency of conjugation of plasmid pS140 did not depend on either the donor or recipient strain. PB - Canadian Science Publishing, Nrc Research Press, Ottawa T2 - Canadian Journal of Microbiology T1 - Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50 EP - 314 IS - 4 SP - 305 VL - 51 DO - 10.1139/W05-009 ER -
@article{ author = "Kojić, Milan and Strahinić, Ivana and Topisirović, Ljubiša", year = "2005", abstract = "Lactococcus lactis subsp. lactis bv. diacetylactis S50 produces a lactococcin A-like bacteriocin named bacteriocin S50, and cell envelope-associated PI-type proteinase activity. This strain harbours 3 small size plasmids: pS6 (6.3 kb), pS7a (7.31 kb), and pS7b (7.27 kb). Plasmid curing using a combination of novobiocin treatment (10 mu g(.)mL(-1)) and sublethal temperature (40 degrees C) resulted in a very low yield (0.17%) of Prt(-), Bac(-), Bac(s) derivatives, which retained all 3 small size resident plasmids. Pulsed-field gel electrophoresis of DNA isolated from the strain S50 and cured derivatives in combination with restriction enzyme analysis and DNA-DNA hybridization revealed that S50 contains 2 additional large plasmids: pS140 (140 kb) and pS80 (80 kb). Conjugation experiments using strain S50 as a donor and various lactococcal recipients resulted in Prt(+), Bac(+), Bac(r) transconjugants. Analysis of these transconjugants strongly indicated that plasmid pS140 harbours the prt and bac genes encoding proteinase and bacteriocin production, and immunity to bacteriocin, since each Prt(+), Bac(+), Bac(r) tranconjugant contained pS140. Accordingly, none of the Prt-, Bac-, Bacs transconjugants contained this plasmid. pS140 was a self-transmissible conjugative plasmid regardless of the host lactococcal recipient used in the test. Frequency of conjugation of plasmid pS140 did not depend on either the donor or recipient strain.", publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa", journal = "Canadian Journal of Microbiology", title = "Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50", pages = "314-305", number = "4", volume = "51", doi = "10.1139/W05-009" }
Kojić, M., Strahinić, I.,& Topisirović, L.. (2005). Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology Canadian Science Publishing, Nrc Research Press, Ottawa., 51(4), 305-314. https://doi.org/10.1139/W05-009
Kojić M, Strahinić I, Topisirović L. Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50. in Canadian Journal of Microbiology. 2005;51(4):305-314. doi:10.1139/W05-009 .
Kojić, Milan, Strahinić, Ivana, Topisirović, Ljubiša, "Proteinase PI and lactococcin A genes are located on the largest plasmid in Lactococcus lactis subsp lactis bv. diacetylactis S50" in Canadian Journal of Microbiology, 51, no. 4 (2005):305-314, https://doi.org/10.1139/W05-009 . .