Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa
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The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/ PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (21) SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies h...ave indicated PsrA as a global regulator.
Keywords:
stationary phase / PsrA regulonSource:
FEMS Microbiology Letters, 2005, 246, 2, 175-181Publisher:
- Wiley-Blackwell, Hoboken
DOI: 10.1016/j.femsle.2005.04.003
ISSN: 0378-1097
PubMed: 15899403
WoS: 000229513800004
Scopus: 2-s2.0-19444366672
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TorlakTY - JOUR AU - Kojić, Milan AU - Jovčić, Branko AU - Vindigni, A AU - Odreman, F AU - Venturi, V PY - 2005 UR - http://intor.torlakinstitut.com/handle/123456789/802 AB - The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/ PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (21) SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator. PB - Wiley-Blackwell, Hoboken T2 - FEMS Microbiology Letters T1 - Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa EP - 181 IS - 2 SP - 175 VL - 246 DO - 10.1016/j.femsle.2005.04.003 ER -
@article{ author = "Kojić, Milan and Jovčić, Branko and Vindigni, A and Odreman, F and Venturi, V", year = "2005", abstract = "The PsrA transcriptional regulator is involved in stationary phase induced transcriptional regulation of rpoS and in negative auto-regulation in Pseudomonas aeruginosa. This study was designed to determine whether other loci were regulated by PsrA in P. aeruginosa. Computer search was performed of the PsrA binding motif (G/CAAAC N2-4 GTTTG/C) against the P. aeruginosa genome sequence. Four of 14 analysed promoters responded to and bound PsrA; (i) divergent promoters controlling PA2952/ PA2951 and PA2953, (ii) promoter of PA0506 and (iii) upstream region of PA3571. Promoters PA0506 and PA2952-PA2951 were regulated negatively whereas promoters of PA2953 and PA3571 were regulated positively by PsrA. Two dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (21) SDS-PAGE) analysis on total proteins from P. aeruginosa PAO1 and psrA knock-out derivative was also performed resulting in the identification of 11 protein spots which were differentially regulated. These studies have indicated PsrA as a global regulator.", publisher = "Wiley-Blackwell, Hoboken", journal = "FEMS Microbiology Letters", title = "Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa", pages = "181-175", number = "2", volume = "246", doi = "10.1016/j.femsle.2005.04.003" }
Kojić, M., Jovčić, B., Vindigni, A., Odreman, F.,& Venturi, V.. (2005). Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa. in FEMS Microbiology Letters Wiley-Blackwell, Hoboken., 246(2), 175-181. https://doi.org/10.1016/j.femsle.2005.04.003
Kojić M, Jovčić B, Vindigni A, Odreman F, Venturi V. Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa. in FEMS Microbiology Letters. 2005;246(2):175-181. doi:10.1016/j.femsle.2005.04.003 .
Kojić, Milan, Jovčić, Branko, Vindigni, A, Odreman, F, Venturi, V, "Novel target genes of PsrA transcriptional regulator of Pseudomonas aeruginosa" in FEMS Microbiology Letters, 246, no. 2 (2005):175-181, https://doi.org/10.1016/j.femsle.2005.04.003 . .