Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia
Authors
Jovčić, BrankoNovović, Katarina
Filipić, Brankica
Velhner, Maja
Todorović, Dalibor
Matović, Kazimir
Rasić, Zoran
Nikolić, Sonja
Kiskarolj, Ferenc
Kojić, Milan
![](/themes/MirageInTOR/images/orcid.png)
Article (Published version)
Metadata
Show full item recordAbstract
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single... nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
Keywords:
WGS / Salmonella / resistance / quinolones / pmrB / colistinSource:
Antibiotics-Basel, 2020, 9, 12Publisher:
- MDPI, Basel
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200031 (Scientific Institute of Veterinary Medicine 'Novi Sad', Novi Sad) (RS-MESTD-inst-2020-200031)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
DOI: 10.3390/antibiotics9120886
ISSN: 2079-6382
WoS: 000601714000001
Scopus: 2-s2.0-85097565355
URI
https://imagine.imgge.bg.ac.rs/handle/123456789/1349http://intor.torlakinstitut.com/handle/123456789/744
Collections
Institution/Community
TorlakTY - JOUR AU - Jovčić, Branko AU - Novović, Katarina AU - Filipić, Brankica AU - Velhner, Maja AU - Todorović, Dalibor AU - Matović, Kazimir AU - Rasić, Zoran AU - Nikolić, Sonja AU - Kiskarolj, Ferenc AU - Kojić, Milan PY - 2020 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/1349 UR - http://intor.torlakinstitut.com/handle/123456789/744 AB - The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia. PB - MDPI, Basel T2 - Antibiotics-Basel T1 - Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia IS - 12 VL - 9 DO - 10.3390/antibiotics9120886 ER -
@article{ author = "Jovčić, Branko and Novović, Katarina and Filipić, Brankica and Velhner, Maja and Todorović, Dalibor and Matović, Kazimir and Rasić, Zoran and Nikolić, Sonja and Kiskarolj, Ferenc and Kojić, Milan", year = "2020", abstract = "The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.", publisher = "MDPI, Basel", journal = "Antibiotics-Basel", title = "Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia", number = "12", volume = "9", doi = "10.3390/antibiotics9120886" }
Jovčić, B., Novović, K., Filipić, B., Velhner, M., Todorović, D., Matović, K., Rasić, Z., Nikolić, S., Kiskarolj, F.,& Kojić, M.. (2020). Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia. in Antibiotics-Basel MDPI, Basel., 9(12). https://doi.org/10.3390/antibiotics9120886
Jovčić B, Novović K, Filipić B, Velhner M, Todorović D, Matović K, Rasić Z, Nikolić S, Kiskarolj F, Kojić M. Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia. in Antibiotics-Basel. 2020;9(12). doi:10.3390/antibiotics9120886 .
Jovčić, Branko, Novović, Katarina, Filipić, Brankica, Velhner, Maja, Todorović, Dalibor, Matović, Kazimir, Rasić, Zoran, Nikolić, Sonja, Kiskarolj, Ferenc, Kojić, Milan, "Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia" in Antibiotics-Basel, 9, no. 12 (2020), https://doi.org/10.3390/antibiotics9120886 . .