Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa
Effects of l.rhamnosus la68 on the immune system of c57 bl/6 mice upon oral administration
Authorized Users Only
2013
Authors
Dimitrijević, RajnaIvanović, Nevena
Mathiesen, Geir
Petrušić, Vladimir
Živković, Irena
Đorđević, Brižita
Dimitrijević, Ljiljana
Conference object (Published version)
Metadata
Show full item recordAbstract
Uvod: Probiotici se koriste vekovima kao deo humane ishrane i u poslednje vreme pridaje im se sve vise značaja. Cilj ovog rada bio je ispitivanje imunoloških aspekata konzumiranja soja Lactobacillus rhamnosus LA68.
Metodologija: Soj LA68 davan je miševima oralno, gavažom četiri puta nedeljno tokom
mesec dana. Korišćena je sveža prekonoćna kultura i jedna doza sastojala se od 2x109
CFU. Nakon isteka eksperimentalnog perioda izolovani su mišji splenociti i analizirani
pomoću protocne citometrije. Meren je i odgovor splenocita u vidu sekrecije citokina
(IFN-y, IL-6, IL-10 and IL-17) kao i proliferacija nakon stimulacije lipopolisaharidom i peptidoglikanom. Analizirani su i citokini u serumu eksperimentalnih miševa. Zdravstveno
stanje životinja praćeno je merenjem težine i osnovnih biohemijskih parametara.
Rezultati: Oralna aplikacija soja LA68 dovodi do značajnog smanjenja zastupljenosti
CD3+, CD25+ i CD19+ celija i do povecanja CD11b+ and CD16/CD32+ ćelija u slezini
eksperimenta...lnih miševa. Detektovana je i povećana osetljivost na stimulaciju merenjem ćelijske proliferacije i sekrecije IL-6. U serumu eksperimentalnih miševa detektovan
je poviseni nivo IFN-y i smanjenje IL-10. Težina životinja i biohemijski parametri nisu se
razlikovali među grupama i ulazili su u opseg normalnih vrednosti za ovu vrstu miša.
Zaključak: Dobijeni rezultati ukazuju na povećanu osetljivost splenocita na stimulaciju,
aktivaciju Th1 tipa imunskog odgovora i pomeranje leukocitne populacije ka monocitno/granulocitnoj populaciji. Detektovane promene mogu pomoći organizmu u odbrani
protiv određenih vrsta infekcija i umanjiti simtome alergije.
Introduction: Probiotic bacteria have been used in human nutrition for centuries and
are attracting more attention in recent years. The aim of this study was to evaluate the
effects of Lactobacillus rhamnosus LA68 on the immune system of mice.
Methodology: In order to examine the immunological aspects of probiotic consumption LA68 was orally administrated via gavage to C57 BL/6 mice. Gavage was performed
four times per week for the duration of one month. Fresh overnight culture was used
and one dose consisted of 2x109 CFU. Upon completion mouse splenocytes were isolated and analyzed by flow cytometry. The magnitude of splenocyte proliferation upon
stimulation with LPS and peptidoglycan and cytokine levels (IFN-y, IL-6, IL-10 and IL-17)
were assessed. Cytokine levels in the serum were also analyzed. Mouse well being was
monitored with weight measurement and basic blood biochemisty.
Results: Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+
and CD19...+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations
in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6
secretion was detected. Increased serum IFN-y and decreased IL-10 levels were found.
Animal weight and biochemical parameters did not differ between the groups and were
within the normal values for this mouse strain.
Conclusion: Our results show increased responsiveness of splenocytes, activation of
the Th1 type of immune response, and a shift of leukocyte populations toward monocyte/granulocyte populations. Changes detected consist of a boost in defense mechanisms against certain types of bacteria and ameliorate allergy symptoms.
Source:
Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija, 2013, 12-Publisher:
- Udruženje za medicinu sporta Srbije
- Univerzitet u Beogradu, Farmaceutski fakultet - Institut za bromatologiju
Collections
Institution/Community
TorlakTY - CONF AU - Dimitrijević, Rajna AU - Ivanović, Nevena AU - Mathiesen, Geir AU - Petrušić, Vladimir AU - Živković, Irena AU - Đorđević, Brižita AU - Dimitrijević, Ljiljana PY - 2013 UR - http://intor.torlakinstitut.com/handle/123456789/712 AB - Uvod: Probiotici se koriste vekovima kao deo humane ishrane i u poslednje vreme pridaje im se sve vise značaja. Cilj ovog rada bio je ispitivanje imunoloških aspekata konzumiranja soja Lactobacillus rhamnosus LA68. Metodologija: Soj LA68 davan je miševima oralno, gavažom četiri puta nedeljno tokom mesec dana. Korišćena je sveža prekonoćna kultura i jedna doza sastojala se od 2x109 CFU. Nakon isteka eksperimentalnog perioda izolovani su mišji splenociti i analizirani pomoću protocne citometrije. Meren je i odgovor splenocita u vidu sekrecije citokina (IFN-y, IL-6, IL-10 and IL-17) kao i proliferacija nakon stimulacije lipopolisaharidom i peptidoglikanom. Analizirani su i citokini u serumu eksperimentalnih miševa. Zdravstveno stanje životinja praćeno je merenjem težine i osnovnih biohemijskih parametara. Rezultati: Oralna aplikacija soja LA68 dovodi do značajnog smanjenja zastupljenosti CD3+, CD25+ i CD19+ celija i do povecanja CD11b+ and CD16/CD32+ ćelija u slezini eksperimentalnih miševa. Detektovana je i povećana osetljivost na stimulaciju merenjem ćelijske proliferacije i sekrecije IL-6. U serumu eksperimentalnih miševa detektovan je poviseni nivo IFN-y i smanjenje IL-10. Težina životinja i biohemijski parametri nisu se razlikovali među grupama i ulazili su u opseg normalnih vrednosti za ovu vrstu miša. Zaključak: Dobijeni rezultati ukazuju na povećanu osetljivost splenocita na stimulaciju, aktivaciju Th1 tipa imunskog odgovora i pomeranje leukocitne populacije ka monocitno/granulocitnoj populaciji. Detektovane promene mogu pomoći organizmu u odbrani protiv određenih vrsta infekcija i umanjiti simtome alergije. AB - Introduction: Probiotic bacteria have been used in human nutrition for centuries and are attracting more attention in recent years. The aim of this study was to evaluate the effects of Lactobacillus rhamnosus LA68 on the immune system of mice. Methodology: In order to examine the immunological aspects of probiotic consumption LA68 was orally administrated via gavage to C57 BL/6 mice. Gavage was performed four times per week for the duration of one month. Fresh overnight culture was used and one dose consisted of 2x109 CFU. Upon completion mouse splenocytes were isolated and analyzed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with LPS and peptidoglycan and cytokine levels (IFN-y, IL-6, IL-10 and IL-17) were assessed. Cytokine levels in the serum were also analyzed. Mouse well being was monitored with weight measurement and basic blood biochemisty. Results: Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-y and decreased IL-10 levels were found. Animal weight and biochemical parameters did not differ between the groups and were within the normal values for this mouse strain. Conclusion: Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leukocyte populations toward monocyte/granulocyte populations. Changes detected consist of a boost in defense mechanisms against certain types of bacteria and ameliorate allergy symptoms. PB - Udruženje za medicinu sporta Srbije PB - Univerzitet u Beogradu, Farmaceutski fakultet - Institut za bromatologiju C3 - Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija T1 - Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa T1 - Effects of l.rhamnosus la68 on the immune system of c57 bl/6 mice upon oral administration SP - 12 UR - https://hdl.handle.net/21.15107/rcub_intor_712 ER -
@conference{ author = "Dimitrijević, Rajna and Ivanović, Nevena and Mathiesen, Geir and Petrušić, Vladimir and Živković, Irena and Đorđević, Brižita and Dimitrijević, Ljiljana", year = "2013", abstract = "Uvod: Probiotici se koriste vekovima kao deo humane ishrane i u poslednje vreme pridaje im se sve vise značaja. Cilj ovog rada bio je ispitivanje imunoloških aspekata konzumiranja soja Lactobacillus rhamnosus LA68. Metodologija: Soj LA68 davan je miševima oralno, gavažom četiri puta nedeljno tokom mesec dana. Korišćena je sveža prekonoćna kultura i jedna doza sastojala se od 2x109 CFU. Nakon isteka eksperimentalnog perioda izolovani su mišji splenociti i analizirani pomoću protocne citometrije. Meren je i odgovor splenocita u vidu sekrecije citokina (IFN-y, IL-6, IL-10 and IL-17) kao i proliferacija nakon stimulacije lipopolisaharidom i peptidoglikanom. Analizirani su i citokini u serumu eksperimentalnih miševa. Zdravstveno stanje životinja praćeno je merenjem težine i osnovnih biohemijskih parametara. Rezultati: Oralna aplikacija soja LA68 dovodi do značajnog smanjenja zastupljenosti CD3+, CD25+ i CD19+ celija i do povecanja CD11b+ and CD16/CD32+ ćelija u slezini eksperimentalnih miševa. Detektovana je i povećana osetljivost na stimulaciju merenjem ćelijske proliferacije i sekrecije IL-6. U serumu eksperimentalnih miševa detektovan je poviseni nivo IFN-y i smanjenje IL-10. Težina životinja i biohemijski parametri nisu se razlikovali među grupama i ulazili su u opseg normalnih vrednosti za ovu vrstu miša. Zaključak: Dobijeni rezultati ukazuju na povećanu osetljivost splenocita na stimulaciju, aktivaciju Th1 tipa imunskog odgovora i pomeranje leukocitne populacije ka monocitno/granulocitnoj populaciji. Detektovane promene mogu pomoći organizmu u odbrani protiv određenih vrsta infekcija i umanjiti simtome alergije., Introduction: Probiotic bacteria have been used in human nutrition for centuries and are attracting more attention in recent years. The aim of this study was to evaluate the effects of Lactobacillus rhamnosus LA68 on the immune system of mice. Methodology: In order to examine the immunological aspects of probiotic consumption LA68 was orally administrated via gavage to C57 BL/6 mice. Gavage was performed four times per week for the duration of one month. Fresh overnight culture was used and one dose consisted of 2x109 CFU. Upon completion mouse splenocytes were isolated and analyzed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with LPS and peptidoglycan and cytokine levels (IFN-y, IL-6, IL-10 and IL-17) were assessed. Cytokine levels in the serum were also analyzed. Mouse well being was monitored with weight measurement and basic blood biochemisty. Results: Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-y and decreased IL-10 levels were found. Animal weight and biochemical parameters did not differ between the groups and were within the normal values for this mouse strain. Conclusion: Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leukocyte populations toward monocyte/granulocyte populations. Changes detected consist of a boost in defense mechanisms against certain types of bacteria and ameliorate allergy symptoms.", publisher = "Udruženje za medicinu sporta Srbije, Univerzitet u Beogradu, Farmaceutski fakultet - Institut za bromatologiju", journal = "Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija", title = "Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa, Effects of l.rhamnosus la68 on the immune system of c57 bl/6 mice upon oral administration", pages = "12", url = "https://hdl.handle.net/21.15107/rcub_intor_712" }
Dimitrijević, R., Ivanović, N., Mathiesen, G., Petrušić, V., Živković, I., Đorđević, B.,& Dimitrijević, L.. (2013). Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa. in Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija Udruženje za medicinu sporta Srbije., 12. https://hdl.handle.net/21.15107/rcub_intor_712
Dimitrijević R, Ivanović N, Mathiesen G, Petrušić V, Živković I, Đorđević B, Dimitrijević L. Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa. in Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija. 2013;:12. https://hdl.handle.net/21.15107/rcub_intor_712 .
Dimitrijević, Rajna, Ivanović, Nevena, Mathiesen, Geir, Petrušić, Vladimir, Živković, Irena, Đorđević, Brižita, Dimitrijević, Ljiljana, "Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa" in Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija (2013):12, https://hdl.handle.net/21.15107/rcub_intor_712 .