5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation
Само за регистроване кориснике
2008
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.
Кључне речи:
translational regulation / rpoS / PseudomonasИзвор:
Journal of Microbiology, 2008, 46, 1, 56-61Издавач:
- Microbiology Soc Korea, Seoul
Финансирање / пројекти:
- Изучавање регулације експресије гена одабраних индустријских микроорганизама (RS-MESTD-MPN2006-2010-143036)
DOI: 10.1007/s12275-007-0127-2
ISSN: 1225-8873
PubMed: 18337694
WoS: 000253545600010
Scopus: 2-s2.0-43249128368
URI
https://imagine.imgge.bg.ac.rs/handle/123456789/333http://intor.torlakinstitut.com/handle/123456789/679
Институција/група
TorlakTY - JOUR AU - Jovčić, Branko AU - Bertani, Iris AU - Venturi, Vittorio AU - Topisirović, Ljubiša AU - Kojić, Milan PY - 2008 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/333 UR - http://intor.torlakinstitut.com/handle/123456789/679 AB - The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene. PB - Microbiology Soc Korea, Seoul T2 - Journal of Microbiology T1 - 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation EP - 61 IS - 1 SP - 56 VL - 46 DO - 10.1007/s12275-007-0127-2 ER -
@article{ author = "Jovčić, Branko and Bertani, Iris and Venturi, Vittorio and Topisirović, Ljubiša and Kojić, Milan", year = "2008", abstract = "The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.", publisher = "Microbiology Soc Korea, Seoul", journal = "Journal of Microbiology", title = "5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation", pages = "61-56", number = "1", volume = "46", doi = "10.1007/s12275-007-0127-2" }
Jovčić, B., Bertani, I., Venturi, V., Topisirović, L.,& Kojić, M.. (2008). 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. in Journal of Microbiology Microbiology Soc Korea, Seoul., 46(1), 56-61. https://doi.org/10.1007/s12275-007-0127-2
Jovčić B, Bertani I, Venturi V, Topisirović L, Kojić M. 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. in Journal of Microbiology. 2008;46(1):56-61. doi:10.1007/s12275-007-0127-2 .
Jovčić, Branko, Bertani, Iris, Venturi, Vittorio, Topisirović, Ljubiša, Kojić, Milan, "5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation" in Journal of Microbiology, 46, no. 1 (2008):56-61, https://doi.org/10.1007/s12275-007-0127-2 . .