Development of RAST assay for determination of anti-Populus canadensis IgE antibodies
Samo za registrovane korisnike
1996
Autori
Trtić, TatjanaVuksanović, L.
Paranos, Svetlana
Petrović, S.
Vujčić, Zoran
Plećaš, Ilija B.
Jankov, Ratko
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.
Izvor:
Journal of Radioanalytical and Nuclear Chemistry-Articles, 1996, 206, 1, 145-149Izdavač:
- Akademiai Kiado Zrt, Budapest
DOI: 10.1007/BF02040051
ISSN: 0236-5731
WoS: A1996VD12500016
Scopus: 2-s2.0-0029834625
Institucija/grupa
TorlakTY - JOUR AU - Trtić, Tatjana AU - Vuksanović, L. AU - Paranos, Svetlana AU - Petrović, S. AU - Vujčić, Zoran AU - Plećaš, Ilija B. AU - Jankov, Ratko PY - 1996 UR - http://intor.torlakinstitut.com/handle/123456789/64 AB - One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides. PB - Akademiai Kiado Zrt, Budapest T2 - Journal of Radioanalytical and Nuclear Chemistry-Articles T1 - Development of RAST assay for determination of anti-Populus canadensis IgE antibodies EP - 149 IS - 1 SP - 145 VL - 206 DO - 10.1007/BF02040051 ER -
@article{ author = "Trtić, Tatjana and Vuksanović, L. and Paranos, Svetlana and Petrović, S. and Vujčić, Zoran and Plećaš, Ilija B. and Jankov, Ratko", year = "1996", abstract = "One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Journal of Radioanalytical and Nuclear Chemistry-Articles", title = "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies", pages = "149-145", number = "1", volume = "206", doi = "10.1007/BF02040051" }
Trtić, T., Vuksanović, L., Paranos, S., Petrović, S., Vujčić, Z., Plećaš, I. B.,& Jankov, R.. (1996). Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry-Articles Akademiai Kiado Zrt, Budapest., 206(1), 145-149. https://doi.org/10.1007/BF02040051
Trtić T, Vuksanović L, Paranos S, Petrović S, Vujčić Z, Plećaš IB, Jankov R. Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry-Articles. 1996;206(1):145-149. doi:10.1007/BF02040051 .
Trtić, Tatjana, Vuksanović, L., Paranos, Svetlana, Petrović, S., Vujčić, Zoran, Plećaš, Ilija B., Jankov, Ratko, "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies" in Journal of Radioanalytical and Nuclear Chemistry-Articles, 206, no. 1 (1996):145-149, https://doi.org/10.1007/BF02040051 . .