The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water
Само за регистроване кориснике
2022
Аутори
Ljubic, VericaMilosevic, Milena
Cvetkovic, Slobodan
Stojanović, Marijana
Novovic, Katarina
Dinic, Miroslav
Popovic, Mina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the r...emoval efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.
Кључне речи:
Exopolysaccharide / L. reuteri B2 / Biosorbent / Ni2+ ion sorption / Adsorption capacityИзвор:
Biomass Conversion and Biorefinery, 2022Издавач:
- Springer
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
Напомена:
- Supplementary information: https://hdl.handle.net/21.15107/rcub_intor_644
Повезане информације:
- Повезани садржај
https://hdl.handle.net/21.15107/rcub_intor_644
DOI: 10.1007/s13399-022-03292-5
ISSN: 2190-6823
WoS: 00085291810000
Scopus: 2-s2.0-85137864112
Институција/група
TorlakTY - JOUR AU - Ljubic, Verica AU - Milosevic, Milena AU - Cvetkovic, Slobodan AU - Stojanović, Marijana AU - Novovic, Katarina AU - Dinic, Miroslav AU - Popovic, Mina PY - 2022 UR - http://intor.torlakinstitut.com/handle/123456789/631 AB - As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments. PB - Springer T2 - Biomass Conversion and Biorefinery T1 - The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water DO - 10.1007/s13399-022-03292-5 ER -
@article{ author = "Ljubic, Verica and Milosevic, Milena and Cvetkovic, Slobodan and Stojanović, Marijana and Novovic, Katarina and Dinic, Miroslav and Popovic, Mina", year = "2022", abstract = "As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.", publisher = "Springer", journal = "Biomass Conversion and Biorefinery", title = "The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water", doi = "10.1007/s13399-022-03292-5" }
Ljubic, V., Milosevic, M., Cvetkovic, S., Stojanović, M., Novovic, K., Dinic, M.,& Popovic, M.. (2022). The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water. in Biomass Conversion and Biorefinery Springer.. https://doi.org/10.1007/s13399-022-03292-5
Ljubic V, Milosevic M, Cvetkovic S, Stojanović M, Novovic K, Dinic M, Popovic M. The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water. in Biomass Conversion and Biorefinery. 2022;. doi:10.1007/s13399-022-03292-5 .
Ljubic, Verica, Milosevic, Milena, Cvetkovic, Slobodan, Stojanović, Marijana, Novovic, Katarina, Dinic, Miroslav, Popovic, Mina, "The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water" in Biomass Conversion and Biorefinery (2022), https://doi.org/10.1007/s13399-022-03292-5 . .