Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells
Authors
Obradović, AnaMatić, Miloš
Ognjanović, Branka
Đurđević, Predrag
Marinković, Emilija
Ušćumlić, Gordana
Božić, Bojan
Božić-Nedeljković, Biljana
Article (Published version)
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In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express... considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
Keywords:
Hydantoin derivatives / Breast cancer cell line MDA-MB-231 / Proliferation / Apoptosis / Cell motility / Nitric oxideSource:
Saudi Pharmaceutical Journal, 2020, 28, 3, 246-254Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Preclinical investigation of bioactive substances (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41010)
- Neuroendocrine control of growth hormone secretion in humans - new challenges. Control of energy homeostasis in humans in various pathological conditions. Genetics in familial pituitary tumorigenesis. Clinical-pathological correlations in atypical pituit (RS-MESTD-Basic Research (BR or ON)-175033)
- Molecular and physiological biomonitoring of aerobic organisms based on the determination of biochemical biomarkers of oxidative stress (RS-MESTD-Basic Research (BR or ON)-173041)
DOI: 10.1016/j.jsps.2020.01.003
ISSN: 1319-0164
PubMed: 32194325
WoS: 000520023600003
Scopus: 2-s2.0-85079047354
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TorlakTY - JOUR AU - Obradović, Ana AU - Matić, Miloš AU - Ognjanović, Branka AU - Đurđević, Predrag AU - Marinković, Emilija AU - Ušćumlić, Gordana AU - Božić, Bojan AU - Božić-Nedeljković, Biljana PY - 2020 UR - http://intor.torlakinstitut.com/handle/123456789/566 AB - In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. PB - Elsevier, Amsterdam T2 - Saudi Pharmaceutical Journal T1 - Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells EP - 254 IS - 3 SP - 246 VL - 28 DO - 10.1016/j.jsps.2020.01.003 ER -
@article{ author = "Obradović, Ana and Matić, Miloš and Ognjanović, Branka and Đurđević, Predrag and Marinković, Emilija and Ušćumlić, Gordana and Božić, Bojan and Božić-Nedeljković, Biljana", year = "2020", abstract = "In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.", publisher = "Elsevier, Amsterdam", journal = "Saudi Pharmaceutical Journal", title = "Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells", pages = "254-246", number = "3", volume = "28", doi = "10.1016/j.jsps.2020.01.003" }
Obradović, A., Matić, M., Ognjanović, B., Đurđević, P., Marinković, E., Ušćumlić, G., Božić, B.,& Božić-Nedeljković, B.. (2020). Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells. in Saudi Pharmaceutical Journal Elsevier, Amsterdam., 28(3), 246-254. https://doi.org/10.1016/j.jsps.2020.01.003
Obradović A, Matić M, Ognjanović B, Đurđević P, Marinković E, Ušćumlić G, Božić B, Božić-Nedeljković B. Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells. in Saudi Pharmaceutical Journal. 2020;28(3):246-254. doi:10.1016/j.jsps.2020.01.003 .
Obradović, Ana, Matić, Miloš, Ognjanović, Branka, Đurđević, Predrag, Marinković, Emilija, Ušćumlić, Gordana, Božić, Bojan, Božić-Nedeljković, Biljana, "Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells" in Saudi Pharmaceutical Journal, 28, no. 3 (2020):246-254, https://doi.org/10.1016/j.jsps.2020.01.003 . .