Optimization, Validation and Standardization of ELISA
Апстракт
The enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical immunochemistry assay based on the specific bond between the antigen and the antibody. The application of this test has significantly changed the practice of medical laboratories in which it is used for detection and quantification of molecules such as hormones, peptides, antibodies, and proteins. Various technical variants of this test can detect antigen (native or foreign) or antibody, determine the intensity of the immune response whether pathological or not; the type of induced immune response as well as the innate immunity potential; and much more. These capabilities, as well as the high sensitivity and robustness of the test and a small price, make it possible to quickly and reliably diagnose diseases in most laboratories. Besides, ELISA is a test that is also used in veterinary medicine, toxicology, allergology, food industry, etc. Despite the fact that it has existed for almost 50 years, different ELISA... tests with different technical solutions are still being developed, which improves and expands the application of the this exceptional test. The aim of this chapter is to empower the rider to optimize, standardize and validate an enzyme linked immunosorbent assay.
Кључне речи:
ELISA / optimization / standardization / validation / accuracy / precisionИзвор:
ELISA Test-Perspectives and Applications, 2020Издавач:
- IntechOpen
Институција/група
TorlakTY - CHAP AU - Živković, Irena AU - Minić, Rajna PY - 2020 UR - http://intor.torlakinstitut.com/handle/123456789/548 AB - The enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical immunochemistry assay based on the specific bond between the antigen and the antibody. The application of this test has significantly changed the practice of medical laboratories in which it is used for detection and quantification of molecules such as hormones, peptides, antibodies, and proteins. Various technical variants of this test can detect antigen (native or foreign) or antibody, determine the intensity of the immune response whether pathological or not; the type of induced immune response as well as the innate immunity potential; and much more. These capabilities, as well as the high sensitivity and robustness of the test and a small price, make it possible to quickly and reliably diagnose diseases in most laboratories. Besides, ELISA is a test that is also used in veterinary medicine, toxicology, allergology, food industry, etc. Despite the fact that it has existed for almost 50 years, different ELISA tests with different technical solutions are still being developed, which improves and expands the application of the this exceptional test. The aim of this chapter is to empower the rider to optimize, standardize and validate an enzyme linked immunosorbent assay. PB - IntechOpen T2 - ELISA Test-Perspectives and Applications T1 - Optimization, Validation and Standardization of ELISA DO - 10.5772/intechopen.94338 ER -
@inbook{ author = "Živković, Irena and Minić, Rajna", year = "2020", abstract = "The enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical immunochemistry assay based on the specific bond between the antigen and the antibody. The application of this test has significantly changed the practice of medical laboratories in which it is used for detection and quantification of molecules such as hormones, peptides, antibodies, and proteins. Various technical variants of this test can detect antigen (native or foreign) or antibody, determine the intensity of the immune response whether pathological or not; the type of induced immune response as well as the innate immunity potential; and much more. These capabilities, as well as the high sensitivity and robustness of the test and a small price, make it possible to quickly and reliably diagnose diseases in most laboratories. Besides, ELISA is a test that is also used in veterinary medicine, toxicology, allergology, food industry, etc. Despite the fact that it has existed for almost 50 years, different ELISA tests with different technical solutions are still being developed, which improves and expands the application of the this exceptional test. The aim of this chapter is to empower the rider to optimize, standardize and validate an enzyme linked immunosorbent assay.", publisher = "IntechOpen", journal = "ELISA Test-Perspectives and Applications", booktitle = "Optimization, Validation and Standardization of ELISA", doi = "10.5772/intechopen.94338" }
Živković, I.,& Minić, R.. (2020). Optimization, Validation and Standardization of ELISA. in ELISA Test-Perspectives and Applications IntechOpen.. https://doi.org/10.5772/intechopen.94338
Živković I, Minić R. Optimization, Validation and Standardization of ELISA. in ELISA Test-Perspectives and Applications. 2020;. doi:10.5772/intechopen.94338 .
Živković, Irena, Minić, Rajna, "Optimization, Validation and Standardization of ELISA" in ELISA Test-Perspectives and Applications (2020), https://doi.org/10.5772/intechopen.94338 . .