Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia
Samo za registrovane korisnike
2015
Autori
Stanojević, MajaNikolić, V.
Stajković, Novica
Stamenković, Gorana
Božović, Bojana
Čekanac, Radovan
Marušić, Predrag
Gligić, Ana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution o...f DOBV in its reservoir hosts in Serbia.
Ključne reči:
Balkans / DOBV / Glis glisIzvor:
Epidemiology and Infection, 2015, 143, 2, 400-404Izdavač:
- Cambridge Univ Press, New York
Finansiranje / projekti:
- Filogenetski pristup analizi molekularne evolucije visoko varijabilnih virusa - koinfekcije, interakcija virusa i domaćina (RS-MESTD-Basic Research (BR or ON)-175024)
DOI: 10.1017/S0950268814001010
ISSN: 0950-2688
PubMed: 24762257
WoS: 000348642600021
Scopus: 2-s2.0-84925230419
Institucija/grupa
TorlakTY - JOUR AU - Stanojević, Maja AU - Nikolić, V. AU - Stajković, Novica AU - Stamenković, Gorana AU - Božović, Bojana AU - Čekanac, Radovan AU - Marušić, Predrag AU - Gligić, Ana PY - 2015 UR - http://intor.torlakinstitut.com/handle/123456789/439 AB - Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia. PB - Cambridge Univ Press, New York T2 - Epidemiology and Infection T1 - Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia EP - 404 IS - 2 SP - 400 VL - 143 DO - 10.1017/S0950268814001010 ER -
@article{ author = "Stanojević, Maja and Nikolić, V. and Stajković, Novica and Stamenković, Gorana and Božović, Bojana and Čekanac, Radovan and Marušić, Predrag and Gligić, Ana", year = "2015", abstract = "Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia.", publisher = "Cambridge Univ Press, New York", journal = "Epidemiology and Infection", title = "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia", pages = "404-400", number = "2", volume = "143", doi = "10.1017/S0950268814001010" }
Stanojević, M., Nikolić, V., Stajković, N., Stamenković, G., Božović, B., Čekanac, R., Marušić, P.,& Gligić, A.. (2015). Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia. in Epidemiology and Infection Cambridge Univ Press, New York., 143(2), 400-404. https://doi.org/10.1017/S0950268814001010
Stanojević M, Nikolić V, Stajković N, Stamenković G, Božović B, Čekanac R, Marušić P, Gligić A. Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia. in Epidemiology and Infection. 2015;143(2):400-404. doi:10.1017/S0950268814001010 .
Stanojević, Maja, Nikolić, V., Stajković, Novica, Stamenković, Gorana, Božović, Bojana, Čekanac, Radovan, Marušić, Predrag, Gligić, Ana, "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia" in Epidemiology and Infection, 143, no. 2 (2015):400-404, https://doi.org/10.1017/S0950268814001010 . .