Ovarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasis
Аутори
Arsenović-Ranin, NevenaKosec, Duško
Nacka-Aleksić, Mirjana
Pilipović, Ivan
Stojić-Vukanić, Zorica
Đikić, Jasmina
Bufan, Biljana
Leposavić, Gordana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The study examined the putative role of ovarian hormones in shaping of rat peripheral T-cell compartment during post-reproductive period. In 20-month-old rats ovariectomized (Ox) at the very end of reproductive period, thymic output, cellularity and composition of major TCR alpha beta+peripheral blood lymphocyte and splenocyte subsets were analyzed. Ovariectomy led to the enlargement of CD8 + peripheral blood lymphocyte and splenocyte subpopulations. This reflected: (i) a more efficient thymic generation of CD8 + cells as indicated by increased number of CD4+CD8+double positive and the most mature CD4CD8+TCR alpha beta(high) thymocytes and CD8 + recent thymic emigrants (RTEs) in peripheral blood, but not in the spleen of Ox rats, and (ii) the expansion of CD8 + memory/activated peripheral blood lymphocytes and splenocytes. The latter was consistent with a greater frequency of proliferating cells among freshly isolated memory/activated CD8 + peripheral blood lymphocytes and splenocytes ...and increased proliferative response of CD8 + splenocytes to stimulation with plate-bound anti-CD3 antibody. The former could be related to the rise in splenic IL-7 and IL-15 mRNA expression. Although ovariectomy affected the overall number of CD4 + T cells in none of the examined compartments, it increased CD4+FoxP3 + peripheral blood lymphocyte and splenocyte counts by enhancing their generation in periphery. Collectively, the results suggest that ovariectomy-induced long-lasting disturbances in ovarian hormone levels (mirrored in diminished progesterone serum level in 20-month-old rats) affects both thymic CD8 + cell generation and peripheral homeostasis and leads to the expansion of CD4+FoxP3 + cells in the periphery, thereby enhancing autoreactive cell control on account of immune system efficacy to combat infections and tumors.
Кључне речи:
Ovarian gland hormones / mature naive T cells / memory/activated T cells / regulatory T cells T-cell proliferation/apoptosisИзвор:
Experimental Biology and Medicine, 2015, 240, 10, 1319-1332Издавач:
- Sage Publications Ltd, London
Финансирање / пројекти:
- Пластичност имунског система током старења: имуномодулаторни потенцијал естрогена (RS-MESTD-Basic Research (BR or ON)-175050)
DOI: 10.1177/1535370215570817
ISSN: 1535-3702
PubMed: 25716018
WoS: 000362190300007
Scopus: 2-s2.0-84942844167
Институција/група
TorlakTY - JOUR AU - Arsenović-Ranin, Nevena AU - Kosec, Duško AU - Nacka-Aleksić, Mirjana AU - Pilipović, Ivan AU - Stojić-Vukanić, Zorica AU - Đikić, Jasmina AU - Bufan, Biljana AU - Leposavić, Gordana PY - 2015 UR - http://intor.torlakinstitut.com/handle/123456789/429 AB - The study examined the putative role of ovarian hormones in shaping of rat peripheral T-cell compartment during post-reproductive period. In 20-month-old rats ovariectomized (Ox) at the very end of reproductive period, thymic output, cellularity and composition of major TCR alpha beta+peripheral blood lymphocyte and splenocyte subsets were analyzed. Ovariectomy led to the enlargement of CD8 + peripheral blood lymphocyte and splenocyte subpopulations. This reflected: (i) a more efficient thymic generation of CD8 + cells as indicated by increased number of CD4+CD8+double positive and the most mature CD4CD8+TCR alpha beta(high) thymocytes and CD8 + recent thymic emigrants (RTEs) in peripheral blood, but not in the spleen of Ox rats, and (ii) the expansion of CD8 + memory/activated peripheral blood lymphocytes and splenocytes. The latter was consistent with a greater frequency of proliferating cells among freshly isolated memory/activated CD8 + peripheral blood lymphocytes and splenocytes and increased proliferative response of CD8 + splenocytes to stimulation with plate-bound anti-CD3 antibody. The former could be related to the rise in splenic IL-7 and IL-15 mRNA expression. Although ovariectomy affected the overall number of CD4 + T cells in none of the examined compartments, it increased CD4+FoxP3 + peripheral blood lymphocyte and splenocyte counts by enhancing their generation in periphery. Collectively, the results suggest that ovariectomy-induced long-lasting disturbances in ovarian hormone levels (mirrored in diminished progesterone serum level in 20-month-old rats) affects both thymic CD8 + cell generation and peripheral homeostasis and leads to the expansion of CD4+FoxP3 + cells in the periphery, thereby enhancing autoreactive cell control on account of immune system efficacy to combat infections and tumors. PB - Sage Publications Ltd, London T2 - Experimental Biology and Medicine T1 - Ovarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasis EP - 1332 IS - 10 SP - 1319 VL - 240 DO - 10.1177/1535370215570817 ER -
@article{ author = "Arsenović-Ranin, Nevena and Kosec, Duško and Nacka-Aleksić, Mirjana and Pilipović, Ivan and Stojić-Vukanić, Zorica and Đikić, Jasmina and Bufan, Biljana and Leposavić, Gordana", year = "2015", abstract = "The study examined the putative role of ovarian hormones in shaping of rat peripheral T-cell compartment during post-reproductive period. In 20-month-old rats ovariectomized (Ox) at the very end of reproductive period, thymic output, cellularity and composition of major TCR alpha beta+peripheral blood lymphocyte and splenocyte subsets were analyzed. Ovariectomy led to the enlargement of CD8 + peripheral blood lymphocyte and splenocyte subpopulations. This reflected: (i) a more efficient thymic generation of CD8 + cells as indicated by increased number of CD4+CD8+double positive and the most mature CD4CD8+TCR alpha beta(high) thymocytes and CD8 + recent thymic emigrants (RTEs) in peripheral blood, but not in the spleen of Ox rats, and (ii) the expansion of CD8 + memory/activated peripheral blood lymphocytes and splenocytes. The latter was consistent with a greater frequency of proliferating cells among freshly isolated memory/activated CD8 + peripheral blood lymphocytes and splenocytes and increased proliferative response of CD8 + splenocytes to stimulation with plate-bound anti-CD3 antibody. The former could be related to the rise in splenic IL-7 and IL-15 mRNA expression. Although ovariectomy affected the overall number of CD4 + T cells in none of the examined compartments, it increased CD4+FoxP3 + peripheral blood lymphocyte and splenocyte counts by enhancing their generation in periphery. Collectively, the results suggest that ovariectomy-induced long-lasting disturbances in ovarian hormone levels (mirrored in diminished progesterone serum level in 20-month-old rats) affects both thymic CD8 + cell generation and peripheral homeostasis and leads to the expansion of CD4+FoxP3 + cells in the periphery, thereby enhancing autoreactive cell control on account of immune system efficacy to combat infections and tumors.", publisher = "Sage Publications Ltd, London", journal = "Experimental Biology and Medicine", title = "Ovarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasis", pages = "1332-1319", number = "10", volume = "240", doi = "10.1177/1535370215570817" }
Arsenović-Ranin, N., Kosec, D., Nacka-Aleksić, M., Pilipović, I., Stojić-Vukanić, Z., Đikić, J., Bufan, B.,& Leposavić, G.. (2015). Ovarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasis. in Experimental Biology and Medicine Sage Publications Ltd, London., 240(10), 1319-1332. https://doi.org/10.1177/1535370215570817
Arsenović-Ranin N, Kosec D, Nacka-Aleksić M, Pilipović I, Stojić-Vukanić Z, Đikić J, Bufan B, Leposavić G. Ovarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasis. in Experimental Biology and Medicine. 2015;240(10):1319-1332. doi:10.1177/1535370215570817 .
Arsenović-Ranin, Nevena, Kosec, Duško, Nacka-Aleksić, Mirjana, Pilipović, Ivan, Stojić-Vukanić, Zorica, Đikić, Jasmina, Bufan, Biljana, Leposavić, Gordana, "Ovarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasis" in Experimental Biology and Medicine, 240, no. 10 (2015):1319-1332, https://doi.org/10.1177/1535370215570817 . .