The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production
Samo za registrovane korisnike
2013
Autori
Dimitrijević, MirjanaStanojević, Stanislava
Kuštrimović, Nataša
Mitić, Katarina
Vujić, Vesna
Aleksić, Iva
Radojević, Katarina
Leposavić, Gordana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations... of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.
Ključne reči:
Aging / Estradiol/progesterone balance / Macrophages / TNF-alpha / NO / RatIzvor:
Experimental Gerontology, 2013, 48, 11, 1243-1254Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Plastičnost imunskog sistema tokom starenja: imunomodulatorni potencijal estrogena (RS-MESTD-Basic Research (BR or ON)-175050)
DOI: 10.1016/j.exger.2013.07.001
ISSN: 0531-5565
PubMed: 23850866
WoS: 000325750900014
Scopus: 2-s2.0-84883649839
Institucija/grupa
TorlakTY - JOUR AU - Dimitrijević, Mirjana AU - Stanojević, Stanislava AU - Kuštrimović, Nataša AU - Mitić, Katarina AU - Vujić, Vesna AU - Aleksić, Iva AU - Radojević, Katarina AU - Leposavić, Gordana PY - 2013 UR - http://intor.torlakinstitut.com/handle/123456789/383 AB - The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Experimental Gerontology T1 - The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production EP - 1254 IS - 11 SP - 1243 VL - 48 DO - 10.1016/j.exger.2013.07.001 ER -
@article{ author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Vujić, Vesna and Aleksić, Iva and Radojević, Katarina and Leposavić, Gordana", year = "2013", abstract = "The phenotype and function of tissue macrophages substantially depend on the cellular milieu and biological effector molecules, such as steroid hormones, to which they are exposed. Furthermore, in female rats, aging is associated with the altered macrophage functioning and the increased estrogen level is followed by a decrease in that of progesterone. Therefore, the present study aimed to investigate the influence of estradiol/progesterone balance on rat macrophage function and phenotype throughout whole adult lifespan. We ovariectomized rats at the late prepubertal age or at the very end of reproductive lifespan, and examined the expression of ED2 (CD163, a marker of mature resident macrophages related to secretion of inflammatory mediators) on peritoneal macrophages and their ability to produce TNF-alpha and NO upon LPS-stimulation at different age points. In addition, to delineate direct and indirect effects of estrogen, we assessed the in vitro influence of different concentrations of 17 beta-estradiol on LPS-induced macrophage TNF-alpha and NO production. Results showed that: ( a) the low frequency of ED2(high) cells amongst peritoneal macrophages of aged rats was accompanied with the reduced TNF-alpha, but not NO production; (b) estradiol level gradually increased following ovariectomy; (c) macrophage ED2 expression and TNF-alpha production were dependent on estradiol/progesterone balance and they changed in the same direction; (d) changes in estradiol/progesterone balance differentially affected macrophages TNF-alpha and NO production; and (e) estradiol exerted pro-inflammatory and anti-inflammatory effects on macrophages in vivo and in vitro, respectively. Overall, our study discloses that estradiol/progesterone balance contributes to the fine-tuning of rat macrophage secretory capacity, and adds to a better understanding of the ovarian steroid hormone role in the regulation of macrophage function, and its significance for the age-associated changes in innate immunity. (C) 2013 Elsevier Inc. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Experimental Gerontology", title = "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production", pages = "1254-1243", number = "11", volume = "48", doi = "10.1016/j.exger.2013.07.001" }
Dimitrijević, M., Stanojević, S., Kuštrimović, N., Mitić, K., Vujić, V., Aleksić, I., Radojević, K.,& Leposavić, G.. (2013). The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology Pergamon-Elsevier Science Ltd, Oxford., 48(11), 1243-1254. https://doi.org/10.1016/j.exger.2013.07.001
Dimitrijević M, Stanojević S, Kuštrimović N, Mitić K, Vujić V, Aleksić I, Radojević K, Leposavić G. The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production. in Experimental Gerontology. 2013;48(11):1243-1254. doi:10.1016/j.exger.2013.07.001 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Vujić, Vesna, Aleksić, Iva, Radojević, Katarina, Leposavić, Gordana, "The influence of aging and estradiol to progesterone ratio on rat macrophage phenotypic profile and NO and TNF-alpha production" in Experimental Gerontology, 48, no. 11 (2013):1243-1254, https://doi.org/10.1016/j.exger.2013.07.001 . .