Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization
Authors
Živković, IrenaStojanović, Marijana
Petrušić, Vladimir
Inić-Kanada, Aleksandra
Mićić, Mileva
Dimitrijević, Ljiljana
Article (Published version)
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We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable to anti-phospholipid antibodies; we explain that this absence results from idiotypic connectivity. Six groups of C57BL/6 mice were hyperimmunized with TTd in aluminum hydroxide or glycerol, with or without pretreatments. Pretreated mice had been injected with polyclonal or nonspecific immune stimulators, such as complete Freund's adjuvant (CFA) or glycerol. The epitope specificity of induced antibodies was tested by indirect ELISA using a tetanus toxoid immunogen and these autoantigens: phospholipids, gangliosides, laminin. Idiotypic connectivity was tested by competitive ELISA and gauged from the degree to which the interaction of idiotypic/anti-idiotypic complementary antibodies was inhibit...ed in the presence of immunized sera antibodies. Higher idiotypic connectivity was noted amongst pretreated mice. There was a positive correlation between higher connectivity and autoantibody levels that acted to favor the participation of natural autoantibodies in the inhibitory process. We conclude that idiotypic connectivity plays a protective role in immunization-induced autoimmunity.
Keywords:
Autoantibodies / C57BL/6 mice / hyperimmunization / networkSource:
Biological Research, 2010, 43, 4, 393-402Publisher:
- Soc Biolgia Chile, Santiago
Funding / projects:
- Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima (RS-MESTD-MPN2006-2010-142020)
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.4067/S0716-97602010000400003
ISSN: 0716-9760
PubMed: 21526265
WoS: 000286413300003
Scopus: 2-s2.0-79851505176
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TorlakTY - JOUR AU - Živković, Irena AU - Stojanović, Marijana AU - Petrušić, Vladimir AU - Inić-Kanada, Aleksandra AU - Mićić, Mileva AU - Dimitrijević, Ljiljana PY - 2010 UR - http://intor.torlakinstitut.com/handle/123456789/306 AB - We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable to anti-phospholipid antibodies; we explain that this absence results from idiotypic connectivity. Six groups of C57BL/6 mice were hyperimmunized with TTd in aluminum hydroxide or glycerol, with or without pretreatments. Pretreated mice had been injected with polyclonal or nonspecific immune stimulators, such as complete Freund's adjuvant (CFA) or glycerol. The epitope specificity of induced antibodies was tested by indirect ELISA using a tetanus toxoid immunogen and these autoantigens: phospholipids, gangliosides, laminin. Idiotypic connectivity was tested by competitive ELISA and gauged from the degree to which the interaction of idiotypic/anti-idiotypic complementary antibodies was inhibited in the presence of immunized sera antibodies. Higher idiotypic connectivity was noted amongst pretreated mice. There was a positive correlation between higher connectivity and autoantibody levels that acted to favor the participation of natural autoantibodies in the inhibitory process. We conclude that idiotypic connectivity plays a protective role in immunization-induced autoimmunity. PB - Soc Biolgia Chile, Santiago T2 - Biological Research T1 - Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization EP - 402 IS - 4 SP - 393 VL - 43 DO - 10.4067/S0716-97602010000400003 ER -
@article{ author = "Živković, Irena and Stojanović, Marijana and Petrušić, Vladimir and Inić-Kanada, Aleksandra and Mićić, Mileva and Dimitrijević, Ljiljana", year = "2010", abstract = "We have already demonstrated (Stojanovic et al., 2009) a connection between tetanus toxoid (TTd) hyperimmunization and the induction of anti-phospholipid syndrome (APS) in BALB/c mice. Here we show that C57BL/6 mice subjected to an identical procedure do not exhibit any like pathology attributable to anti-phospholipid antibodies; we explain that this absence results from idiotypic connectivity. Six groups of C57BL/6 mice were hyperimmunized with TTd in aluminum hydroxide or glycerol, with or without pretreatments. Pretreated mice had been injected with polyclonal or nonspecific immune stimulators, such as complete Freund's adjuvant (CFA) or glycerol. The epitope specificity of induced antibodies was tested by indirect ELISA using a tetanus toxoid immunogen and these autoantigens: phospholipids, gangliosides, laminin. Idiotypic connectivity was tested by competitive ELISA and gauged from the degree to which the interaction of idiotypic/anti-idiotypic complementary antibodies was inhibited in the presence of immunized sera antibodies. Higher idiotypic connectivity was noted amongst pretreated mice. There was a positive correlation between higher connectivity and autoantibody levels that acted to favor the participation of natural autoantibodies in the inhibitory process. We conclude that idiotypic connectivity plays a protective role in immunization-induced autoimmunity.", publisher = "Soc Biolgia Chile, Santiago", journal = "Biological Research", title = "Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization", pages = "402-393", number = "4", volume = "43", doi = "10.4067/S0716-97602010000400003" }
Živković, I., Stojanović, M., Petrušić, V., Inić-Kanada, A., Mićić, M.,& Dimitrijević, L.. (2010). Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization. in Biological Research Soc Biolgia Chile, Santiago., 43(4), 393-402. https://doi.org/10.4067/S0716-97602010000400003
Živković I, Stojanović M, Petrušić V, Inić-Kanada A, Mićić M, Dimitrijević L. Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization. in Biological Research. 2010;43(4):393-402. doi:10.4067/S0716-97602010000400003 .
Živković, Irena, Stojanović, Marijana, Petrušić, Vladimir, Inić-Kanada, Aleksandra, Mićić, Mileva, Dimitrijević, Ljiljana, "Network connectivity is shown to change in C57BL/6 mice during a continuing immune response subsequent to tetanus toxoid hyperimmunization" in Biological Research, 43, no. 4 (2010):393-402, https://doi.org/10.4067/S0716-97602010000400003 . .