The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response
Аутори
Stojanović, MarijanaŽivković, Irena
Inić-Kanada, Aleksandra
Petrušić, Vladimir
Mićić, Mileva
Dimitrijević, Ljiljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Results are presented concerning our attempts to create a suitable model system for studying the connection between microbial antigen (micAg), autoimmunity and autoimmune disease on the basis of hyper-immunization and application of micAg in different contexts. Our research was focused on tetanus toxoid (TTd) as a model micAg. Non-pretreated and complete Freund's adjuvant pretreated BALB/c mice were immunized with high doses of TTd mixed with glycerol or aluminum hydroxide as adjuvants. The main aims of the experiments were to evaluate the properties of induced humoral immune responses, evaluate the pathological potential of induced immune responses and determine possible correlations between the properties of a humoral immune response and its pathological potential. The production of TTd-specific and self-reactive beta(2)-glycoprotein I (beta(2)-GP I)-specific antibodies (Abs) was detected in all groups but with specific, context-related properties. Analysis of pregnancy-related patho...logy (anti-beta(2)-GP I Abs-associated) showed differences in the pathological potential of the induced immune response. It was demonstrated that severity of pathology is positively correlated to the abundance of IgG that recognizes beta(2)-GP I adsorbed onto phosphatidylserine, and to IgG affinity. Furthermore, it was demonstrated that molecular mimicry, which results in generation of anti-beta(2)-GP I Abs upon TTd immunization, is necessary but not sufficient for the development of pregnancy-related pathology.
Кључне речи:
adjuvant / anti-beta(2)-glycoprotein I antibody / tetanus toxoidИзвор:
Microbiology and Immunology, 2009, 53, 2, 89-100Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Испитивање структуре и функције биолошки важних макромолекула у физиолошким и патолошким стањима (RS-142020)
DOI: 10.1111/j.1348-0421.2008.00094.x
ISSN: 0385-5600
PubMed: 19291092
WoS: 000263853200005
Scopus: 2-s2.0-64049108541
Институција/група
TorlakTY - JOUR AU - Stojanović, Marijana AU - Živković, Irena AU - Inić-Kanada, Aleksandra AU - Petrušić, Vladimir AU - Mićić, Mileva AU - Dimitrijević, Ljiljana PY - 2009 UR - http://intor.torlakinstitut.com/handle/123456789/284 AB - Results are presented concerning our attempts to create a suitable model system for studying the connection between microbial antigen (micAg), autoimmunity and autoimmune disease on the basis of hyper-immunization and application of micAg in different contexts. Our research was focused on tetanus toxoid (TTd) as a model micAg. Non-pretreated and complete Freund's adjuvant pretreated BALB/c mice were immunized with high doses of TTd mixed with glycerol or aluminum hydroxide as adjuvants. The main aims of the experiments were to evaluate the properties of induced humoral immune responses, evaluate the pathological potential of induced immune responses and determine possible correlations between the properties of a humoral immune response and its pathological potential. The production of TTd-specific and self-reactive beta(2)-glycoprotein I (beta(2)-GP I)-specific antibodies (Abs) was detected in all groups but with specific, context-related properties. Analysis of pregnancy-related pathology (anti-beta(2)-GP I Abs-associated) showed differences in the pathological potential of the induced immune response. It was demonstrated that severity of pathology is positively correlated to the abundance of IgG that recognizes beta(2)-GP I adsorbed onto phosphatidylserine, and to IgG affinity. Furthermore, it was demonstrated that molecular mimicry, which results in generation of anti-beta(2)-GP I Abs upon TTd immunization, is necessary but not sufficient for the development of pregnancy-related pathology. PB - Wiley, Hoboken T2 - Microbiology and Immunology T1 - The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response EP - 100 IS - 2 SP - 89 VL - 53 DO - 10.1111/j.1348-0421.2008.00094.x ER -
@article{ author = "Stojanović, Marijana and Živković, Irena and Inić-Kanada, Aleksandra and Petrušić, Vladimir and Mićić, Mileva and Dimitrijević, Ljiljana", year = "2009", abstract = "Results are presented concerning our attempts to create a suitable model system for studying the connection between microbial antigen (micAg), autoimmunity and autoimmune disease on the basis of hyper-immunization and application of micAg in different contexts. Our research was focused on tetanus toxoid (TTd) as a model micAg. Non-pretreated and complete Freund's adjuvant pretreated BALB/c mice were immunized with high doses of TTd mixed with glycerol or aluminum hydroxide as adjuvants. The main aims of the experiments were to evaluate the properties of induced humoral immune responses, evaluate the pathological potential of induced immune responses and determine possible correlations between the properties of a humoral immune response and its pathological potential. The production of TTd-specific and self-reactive beta(2)-glycoprotein I (beta(2)-GP I)-specific antibodies (Abs) was detected in all groups but with specific, context-related properties. Analysis of pregnancy-related pathology (anti-beta(2)-GP I Abs-associated) showed differences in the pathological potential of the induced immune response. It was demonstrated that severity of pathology is positively correlated to the abundance of IgG that recognizes beta(2)-GP I adsorbed onto phosphatidylserine, and to IgG affinity. Furthermore, it was demonstrated that molecular mimicry, which results in generation of anti-beta(2)-GP I Abs upon TTd immunization, is necessary but not sufficient for the development of pregnancy-related pathology.", publisher = "Wiley, Hoboken", journal = "Microbiology and Immunology", title = "The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response", pages = "100-89", number = "2", volume = "53", doi = "10.1111/j.1348-0421.2008.00094.x" }
Stojanović, M., Živković, I., Inić-Kanada, A., Petrušić, V., Mićić, M.,& Dimitrijević, L.. (2009). The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response. in Microbiology and Immunology Wiley, Hoboken., 53(2), 89-100. https://doi.org/10.1111/j.1348-0421.2008.00094.x
Stojanović M, Živković I, Inić-Kanada A, Petrušić V, Mićić M, Dimitrijević L. The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response. in Microbiology and Immunology. 2009;53(2):89-100. doi:10.1111/j.1348-0421.2008.00094.x .
Stojanović, Marijana, Živković, Irena, Inić-Kanada, Aleksandra, Petrušić, Vladimir, Mićić, Mileva, Dimitrijević, Ljiljana, "The context of tetanus toxoid application influences the outcome of antigen-specific and self-directed humoral immune response" in Microbiology and Immunology, 53, no. 2 (2009):89-100, https://doi.org/10.1111/j.1348-0421.2008.00094.x . .