Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat
Само за регистроване кориснике
2008
Аутори
Erić-Jovičić, MilenaPopović, Miroljub
Jovanova-Nešić, Katica
Popović, Natalija
Jovičić-Pavlović, Svetlana
Rakić, Ljubisav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Several studies suggested that the activity of erythrocyte Na, K-ATPase declines with aging. Here, it is postulated that alterations in the substrate kinetics of the erythrocyte membrane Na, K-ATPase could be more aggravated in conditions of brain cholinergic dysfunction seen in Alzheimer's disease than in normal aging. To test this hypothesis, we compared the Na, K-ATPase activity (Vmax/Km parameters) in aged rats with those in young rats with brain cholinergic dysfunction induced by electrolytic-, kainic acid-lesioned nucleus basalis magnocellularis (NBM) or by intracerebroventricular AlCl3 administration. In the above mentioned groups, Vmax values were significantly lower in comparison to the control animals. Furthermore, Km values were significantly higher in animals with electrolytic-induced NBM lesions, AlCl3 treated rats and aged animals. However, Km was significantly lower in kainic acid-induced NBM lesions compared to the control group. The Na, K-ATPase catalytic efficiency, e...stimated by the ratio Vm/Km, decreased as followed: young animals gt aged animals gt kainic acid lesion gt electrolityc lesion gt AlCl3. Our data suggest that neurodegenerative processes similar to those seen in Alzheimer's disease affect the sodium/potassium pump functionality which might be detected in peripheral blood erythrocyte membranes.
Кључне речи:
aging / aluminium / Alzheimer's disease / K-ATPase / Na / nucleus basalis magnocellularis / ratsИзвор:
Journal of Alzheimer's Disease, 2008, 14, 1, 85-93Издавач:
- IOS Press, Amsterdam
DOI: 10.3233/JAD-2008-14108
ISSN: 1387-2877
PubMed: 18525130
WoS: 000256624300008
Scopus: 2-s2.0-47849119470
Институција/група
TorlakTY - JOUR AU - Erić-Jovičić, Milena AU - Popović, Miroljub AU - Jovanova-Nešić, Katica AU - Popović, Natalija AU - Jovičić-Pavlović, Svetlana AU - Rakić, Ljubisav PY - 2008 UR - http://intor.torlakinstitut.com/handle/123456789/247 AB - Several studies suggested that the activity of erythrocyte Na, K-ATPase declines with aging. Here, it is postulated that alterations in the substrate kinetics of the erythrocyte membrane Na, K-ATPase could be more aggravated in conditions of brain cholinergic dysfunction seen in Alzheimer's disease than in normal aging. To test this hypothesis, we compared the Na, K-ATPase activity (Vmax/Km parameters) in aged rats with those in young rats with brain cholinergic dysfunction induced by electrolytic-, kainic acid-lesioned nucleus basalis magnocellularis (NBM) or by intracerebroventricular AlCl3 administration. In the above mentioned groups, Vmax values were significantly lower in comparison to the control animals. Furthermore, Km values were significantly higher in animals with electrolytic-induced NBM lesions, AlCl3 treated rats and aged animals. However, Km was significantly lower in kainic acid-induced NBM lesions compared to the control group. The Na, K-ATPase catalytic efficiency, estimated by the ratio Vm/Km, decreased as followed: young animals gt aged animals gt kainic acid lesion gt electrolityc lesion gt AlCl3. Our data suggest that neurodegenerative processes similar to those seen in Alzheimer's disease affect the sodium/potassium pump functionality which might be detected in peripheral blood erythrocyte membranes. PB - IOS Press, Amsterdam T2 - Journal of Alzheimer's Disease T1 - Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat EP - 93 IS - 1 SP - 85 VL - 14 DO - 10.3233/JAD-2008-14108 ER -
@article{ author = "Erić-Jovičić, Milena and Popović, Miroljub and Jovanova-Nešić, Katica and Popović, Natalija and Jovičić-Pavlović, Svetlana and Rakić, Ljubisav", year = "2008", abstract = "Several studies suggested that the activity of erythrocyte Na, K-ATPase declines with aging. Here, it is postulated that alterations in the substrate kinetics of the erythrocyte membrane Na, K-ATPase could be more aggravated in conditions of brain cholinergic dysfunction seen in Alzheimer's disease than in normal aging. To test this hypothesis, we compared the Na, K-ATPase activity (Vmax/Km parameters) in aged rats with those in young rats with brain cholinergic dysfunction induced by electrolytic-, kainic acid-lesioned nucleus basalis magnocellularis (NBM) or by intracerebroventricular AlCl3 administration. In the above mentioned groups, Vmax values were significantly lower in comparison to the control animals. Furthermore, Km values were significantly higher in animals with electrolytic-induced NBM lesions, AlCl3 treated rats and aged animals. However, Km was significantly lower in kainic acid-induced NBM lesions compared to the control group. The Na, K-ATPase catalytic efficiency, estimated by the ratio Vm/Km, decreased as followed: young animals gt aged animals gt kainic acid lesion gt electrolityc lesion gt AlCl3. Our data suggest that neurodegenerative processes similar to those seen in Alzheimer's disease affect the sodium/potassium pump functionality which might be detected in peripheral blood erythrocyte membranes.", publisher = "IOS Press, Amsterdam", journal = "Journal of Alzheimer's Disease", title = "Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat", pages = "93-85", number = "1", volume = "14", doi = "10.3233/JAD-2008-14108" }
Erić-Jovičić, M., Popović, M., Jovanova-Nešić, K., Popović, N., Jovičić-Pavlović, S.,& Rakić, L.. (2008). Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat. in Journal of Alzheimer's Disease IOS Press, Amsterdam., 14(1), 85-93. https://doi.org/10.3233/JAD-2008-14108
Erić-Jovičić M, Popović M, Jovanova-Nešić K, Popović N, Jovičić-Pavlović S, Rakić L. Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat. in Journal of Alzheimer's Disease. 2008;14(1):85-93. doi:10.3233/JAD-2008-14108 .
Erić-Jovičić, Milena, Popović, Miroljub, Jovanova-Nešić, Katica, Popović, Natalija, Jovičić-Pavlović, Svetlana, Rakić, Ljubisav, "Aging, aluminium and basal forebrain lesions modify substrate kinetics of erythrocyte membrane Na, K-ATPase in the rat" in Journal of Alzheimer's Disease, 14, no. 1 (2008):85-93, https://doi.org/10.3233/JAD-2008-14108 . .