Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method
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2007
Authors
Blanuša, MilanPerović, Iva
Popović, Milica
Polović, Natalija
Burazer, Lidija
Milovanović, Mina
Gavrović-Jankulović, Marija
Jankov, Ratko
Ćirković-Veličković, Tanja
Article (Published version)
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Show full item recordAbstract
A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study here theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use. (c) 2007 Elsevier B.V. All rights reserved.
Keywords:
actinidin / act c 1 / allergen / art v 1 / ion-exchange / HPLC / quantification / kiwi fruit / mugwort / pollenSource:
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2007, 857, 2, 188-194Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima (RS-MESTD-MPN2006-2010-142020)
DOI: 10.1016/j.jchromb.2007.07.015
ISSN: 1570-0232
PubMed: 17652037
WoS: 000250211500002
Scopus: 2-s2.0-34548823476
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TorlakTY - JOUR AU - Blanuša, Milan AU - Perović, Iva AU - Popović, Milica AU - Polović, Natalija AU - Burazer, Lidija AU - Milovanović, Mina AU - Gavrović-Jankulović, Marija AU - Jankov, Ratko AU - Ćirković-Veličković, Tanja PY - 2007 UR - http://intor.torlakinstitut.com/handle/123456789/224 AB - A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study here theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use. (c) 2007 Elsevier B.V. All rights reserved. PB - Elsevier Science Bv, Amsterdam T2 - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences T1 - Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method EP - 194 IS - 2 SP - 188 VL - 857 DO - 10.1016/j.jchromb.2007.07.015 ER -
@article{ author = "Blanuša, Milan and Perović, Iva and Popović, Milica and Polović, Natalija and Burazer, Lidija and Milovanović, Mina and Gavrović-Jankulović, Marija and Jankov, Ratko and Ćirković-Veličković, Tanja", year = "2007", abstract = "A simple ion-exchange HPLC-UV method was developed for determination of major allergens from mugwort pollen and kiwi fruit extracts in mass-units. The separation of Art v 1 and Act c 1 from other components in the extracts was achieved in one step. The extinction coefficients used in the study here theoretically determined and compared to the extinction coefficients determined by gravimetry. We also reported a close correlation of the major allergen contents with the overall allergenic potency of the extracts determined by inhibition ELISA. This method could be a useful tool for standardization of allergenic extracts for clinical use. (c) 2007 Elsevier B.V. All rights reserved.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences", title = "Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method", pages = "194-188", number = "2", volume = "857", doi = "10.1016/j.jchromb.2007.07.015" }
Blanuša, M., Perović, I., Popović, M., Polović, N., Burazer, L., Milovanović, M., Gavrović-Jankulović, M., Jankov, R.,& Ćirković-Veličković, T.. (2007). Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences Elsevier Science Bv, Amsterdam., 857(2), 188-194. https://doi.org/10.1016/j.jchromb.2007.07.015
Blanuša M, Perović I, Popović M, Polović N, Burazer L, Milovanović M, Gavrović-Jankulović M, Jankov R, Ćirković-Veličković T. Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2007;857(2):188-194. doi:10.1016/j.jchromb.2007.07.015 .
Blanuša, Milan, Perović, Iva, Popović, Milica, Polović, Natalija, Burazer, Lidija, Milovanović, Mina, Gavrović-Jankulović, Marija, Jankov, Ratko, Ćirković-Veličković, Tanja, "Quantification of Art v 1 and Act c 1 being major allergens of mugwort pollen and kiwi fruit extracts in mass-units by ion-exchange HPLC-UV method" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 857, no. 2 (2007):188-194, https://doi.org/10.1016/j.jchromb.2007.07.015 . .