Pseudomonas aeruginosa and reserve drugs

Abstract

This investigation tested 312 strains of pseudomonas aeruginosa. Out of them 70% were urine isolates, and 30% were taken from different pathogenic materials. Sensitivity of P. aeruginosa was tested to: penicillin, amikacin ceftazidime, ciprofloxacin, ofloxacin and fleroxacin. In order to test sensitivity of P. aeruginosa to reserve drugs, agar-dilution method was used, and its sensitivity was expressed with minimal inhibitory concentration (MIC). On the basis of our results 93.6% of strains were susceptible to piperacillin, and only 6.4% were resistant, with MIC50 6,3 μg/ml. High susceptibility was also established to ceftazidime (82%, with 10.6% resistant strains and MIC50 3,1 μg/ml) so this drug can also be used as a drug of choice in for P. aeruginosa. Good susceptibility was established to amikacin (60.2%, and 33.6% resistant strains with MIC50 12,5 μg/ml). Moderate susceptibility was established to ciprofloxacin (45.8% susceptible strains and 49.3% resistant strains with MIC50 1,56 μg/ml). Poor susceptibility was established to ofloxacin and fleroxacin with 57.4% resistant strains to ofloxacin and 59% to fleroxacin; moderate susceptibility to ofloxacin was established for 25.6% of strains and to fleroxacin for 30.4%; only 17% of strains were ofloxacin susceptible and 10.6% fleroxacin susceptible, with MIC50 6,3 μg/ml, discrediting both drugs as antipseudomonas drugs.

U radu je testirano 312 sojeva Ps. aeruginosa. Od toga 70 % izolata potiče iz urina, a preostalih 30% potiče iz različitog patološkog materijala. Osetljivost Ps. aeruginosa ispitivana je prema: Piperacilinu, Amikacinu Ceftazidimu, Ciprofloksacinu, Ofloksacinu i Fleroksacinu. Za ispitivanje osetljivosti Ps. aeruginosa na rezervne lekove, korišćen je agar-dilucioni metod, pri čemu je osetljivost ove bakterije izražena minimalnom inhibitornom koncentracijom (MIC). Na osnovu našeg rada vodeći među testiranim antibioticima je antipseudomonasni lek Piperacilin sa 93,6% piperacilin osetljivih sojeva i svega 6,4% piperacilin rezistentnih sojeva i MIC50 6,3mg/ml. Visoku osetljivost ispitivani sojevi Ps. aeruginosa pokazali su prema Ceftazidimu sa 82% Ceftazidim osetljivih i svega 10,6% Ceftazidim rezistentnih sojeva i MIC50 3,1mg/ml, te se i on može koristiti kao lek izbora za Ps. aeruginosa. Dobru osetljivost pokazali su ispitivani sojevi prema Amikacinu 60,2% je Amikacin osetljivih sojeva i 33,6% Amikacin rezistentnih sojeva sa MIC50 12,5mg/ml. Umerenu osetljivost pokazali su ispitivani sojevi prema Ciprofloksacinu sa 45,8% osetljivih i 49,3% rezistentnih sojeva i MIC50 1,56mg/ml. Slabu osetljivost pokazali su ispitivani sojevi prema Ofloksacinu i Fleroksacinu, pri čemu je Ofloksacin rezistentnih sojeva 57,4%, odnosno Fleroksacin rezistentnih 59%; umereno Ofloksacin osetljivih 25,6%, odnosno umereno Fleroksacin osetljivih 30,4% i svega Ofloksacin osetljivih 17%, odnosno Fleroksacin osetljivih 10,6% a MIC50 Ofloksacina i Fleroksacina je 6,3mg/ml, što ova dva leka i diskredituje iz grupe antipseudomonasnih lekova.