Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration
Само за регистроване кориснике
2004
Аутори
Šeatović, Saša S.Inić-Kanada, Aleksandra
Stojanović, Marijana
Živković, Irena
Jankov, Ratko
Dimitrijević, Ljiljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
According to the recommendation of the World Health Organization (WHO), the use of an in vivo test for measuring of the potency of tetanus toxoid vaccine (TTdV) is still unavoidable, but the establishment of a convenient in vitro test would significantly improve the work in this field. A sandwich enzyme-linked immunosorbent assay (sELISA) was developed for a rapid and sensitive quantification of tetanus toxoid (TTd). We produced four monoclonal antibodies (MAbs) designated 41, 51, 62, and 71 that reacted with TTd and recognized, different antigenic determinants on TTd. We also used two of these antibodies for developing a sELISA, with MoAb 71 as an immobilized and MoAb 51 as a capture antibody. The measurement range of this assay was from 31-1000 ng/mL and the minimum detection limit for TTd was 31 ng/mL. This high sensitivity of this sELISA and its good reproducibility suggest that the developed method could be reliably used to estimate the concentration of TTd, which could be easily ...extrapolated to the estimation of vaccine potency.
Кључне речи:
sandwich ELISA / tetanus toxoid / monoclonal antibodiesИзвор:
Journal of Immunoassay & Immunochemistry, 2004, 25, 1, 31-44Издавач:
- Taylor & Francis Inc, Philadelphia
DOI: 10.1081/IAS-120027224
ISSN: 1532-1819
PubMed: 15038615
WoS: 000220152400003
Scopus: 2-s2.0-18844466254
Институција/група
TorlakTY - JOUR AU - Šeatović, Saša S. AU - Inić-Kanada, Aleksandra AU - Stojanović, Marijana AU - Živković, Irena AU - Jankov, Ratko AU - Dimitrijević, Ljiljana PY - 2004 UR - http://intor.torlakinstitut.com/handle/123456789/181 AB - According to the recommendation of the World Health Organization (WHO), the use of an in vivo test for measuring of the potency of tetanus toxoid vaccine (TTdV) is still unavoidable, but the establishment of a convenient in vitro test would significantly improve the work in this field. A sandwich enzyme-linked immunosorbent assay (sELISA) was developed for a rapid and sensitive quantification of tetanus toxoid (TTd). We produced four monoclonal antibodies (MAbs) designated 41, 51, 62, and 71 that reacted with TTd and recognized, different antigenic determinants on TTd. We also used two of these antibodies for developing a sELISA, with MoAb 71 as an immobilized and MoAb 51 as a capture antibody. The measurement range of this assay was from 31-1000 ng/mL and the minimum detection limit for TTd was 31 ng/mL. This high sensitivity of this sELISA and its good reproducibility suggest that the developed method could be reliably used to estimate the concentration of TTd, which could be easily extrapolated to the estimation of vaccine potency. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Immunoassay & Immunochemistry T1 - Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration EP - 44 IS - 1 SP - 31 VL - 25 DO - 10.1081/IAS-120027224 ER -
@article{ author = "Šeatović, Saša S. and Inić-Kanada, Aleksandra and Stojanović, Marijana and Živković, Irena and Jankov, Ratko and Dimitrijević, Ljiljana", year = "2004", abstract = "According to the recommendation of the World Health Organization (WHO), the use of an in vivo test for measuring of the potency of tetanus toxoid vaccine (TTdV) is still unavoidable, but the establishment of a convenient in vitro test would significantly improve the work in this field. A sandwich enzyme-linked immunosorbent assay (sELISA) was developed for a rapid and sensitive quantification of tetanus toxoid (TTd). We produced four monoclonal antibodies (MAbs) designated 41, 51, 62, and 71 that reacted with TTd and recognized, different antigenic determinants on TTd. We also used two of these antibodies for developing a sELISA, with MoAb 71 as an immobilized and MoAb 51 as a capture antibody. The measurement range of this assay was from 31-1000 ng/mL and the minimum detection limit for TTd was 31 ng/mL. This high sensitivity of this sELISA and its good reproducibility suggest that the developed method could be reliably used to estimate the concentration of TTd, which could be easily extrapolated to the estimation of vaccine potency.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Immunoassay & Immunochemistry", title = "Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration", pages = "44-31", number = "1", volume = "25", doi = "10.1081/IAS-120027224" }
Šeatović, S. S., Inić-Kanada, A., Stojanović, M., Živković, I., Jankov, R.,& Dimitrijević, L.. (2004). Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration. in Journal of Immunoassay & Immunochemistry Taylor & Francis Inc, Philadelphia., 25(1), 31-44. https://doi.org/10.1081/IAS-120027224
Šeatović SS, Inić-Kanada A, Stojanović M, Živković I, Jankov R, Dimitrijević L. Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration. in Journal of Immunoassay & Immunochemistry. 2004;25(1):31-44. doi:10.1081/IAS-120027224 .
Šeatović, Saša S., Inić-Kanada, Aleksandra, Stojanović, Marijana, Živković, Irena, Jankov, Ratko, Dimitrijević, Ljiljana, "Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration" in Journal of Immunoassay & Immunochemistry, 25, no. 1 (2004):31-44, https://doi.org/10.1081/IAS-120027224 . .